Life on Mars
... electric current is passed through the gel, and because the DNA fragments are negatively charged, they are drawn towards the positive electrode. The smaller the DNA fragment, the faster it travels along the gel. This results in DNA separation by size, with the smaller fragments migrating further tha ...
... electric current is passed through the gel, and because the DNA fragments are negatively charged, they are drawn towards the positive electrode. The smaller the DNA fragment, the faster it travels along the gel. This results in DNA separation by size, with the smaller fragments migrating further tha ...
catalytically active (used for assay)
... loaded on the column at 3 mL/minute on an AKTA-express system (GE/Amersham). The column was then washed with 20 column volumes of lysis buffer, and 10 volumes of wash buffer and then eluted with elution buffer. The eluted peak of A280 was automatically collected. Column 2: Gel Filtration, Hiload 16/ ...
... loaded on the column at 3 mL/minute on an AKTA-express system (GE/Amersham). The column was then washed with 20 column volumes of lysis buffer, and 10 volumes of wash buffer and then eluted with elution buffer. The eluted peak of A280 was automatically collected. Column 2: Gel Filtration, Hiload 16/ ...
Manipulating DNA Notes
... electrophoresis is used to separate DNA pieces – GEL ELECTROPHORESIS: procedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel ...
... electrophoresis is used to separate DNA pieces – GEL ELECTROPHORESIS: procedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel ...
Restriction fragment length polymorphism
... Characterization of transformants, Study of genome Organization and phylogenic analysis. Paternity testing and the investigation of crimes. Measure the genomic response to selection in ...
... Characterization of transformants, Study of genome Organization and phylogenic analysis. Paternity testing and the investigation of crimes. Measure the genomic response to selection in ...
DNA
... molecules of DNA and RNA. [When an electric current is applied to the gel, negatively charged molecules move toward the positive end, and positively charged molecules move toward the negative end.] The charge, size, and shape of a particular molecule all affect the rate at which a molecule moves thr ...
... molecules of DNA and RNA. [When an electric current is applied to the gel, negatively charged molecules move toward the positive end, and positively charged molecules move toward the negative end.] The charge, size, and shape of a particular molecule all affect the rate at which a molecule moves thr ...
Detailed description of PA
... The released DNA was end repaired by 3 units T4 DNA polymerase (NEB) in 1x NEB buffer 2 and 300 µM dNTP (Bioline). The reaction was incubated at 15 °C for 15 min, followed by purification with ZYMO clean & concentrator-5 kit. Eluted DNA was A-tailed by Klenow (exo-) DNA polymerase (Epicentre) with 2 ...
... The released DNA was end repaired by 3 units T4 DNA polymerase (NEB) in 1x NEB buffer 2 and 300 µM dNTP (Bioline). The reaction was incubated at 15 °C for 15 min, followed by purification with ZYMO clean & concentrator-5 kit. Eluted DNA was A-tailed by Klenow (exo-) DNA polymerase (Epicentre) with 2 ...
Bio100-Bio lab outline
... Will tell them about the blue white screening that they will be doing with the transformed cells. 5. Spreading of transformed bacteria & incubating the plates at 37ºC over night. (15min) Week 3: 1. Plasmid Isolation (Mini Prep) from the day 4’s inoculums. (1.30hr) TA’s will inoculate the white c ...
... Will tell them about the blue white screening that they will be doing with the transformed cells. 5. Spreading of transformed bacteria & incubating the plates at 37ºC over night. (15min) Week 3: 1. Plasmid Isolation (Mini Prep) from the day 4’s inoculums. (1.30hr) TA’s will inoculate the white c ...
Biochem BIG IDEAS - Canvas by Instructure
... (A-T or A-U )and cytosine pairs with guanine (C-G). i. Purines (G and A) have a double ring structure. ii. Pyrimidines (C, T and U) have a single ring structure. 4. The sequence of the RNA bases, together with the structure of the RNA molecule, determines RNA function (more in DNA unit) i. mRNA carr ...
... (A-T or A-U )and cytosine pairs with guanine (C-G). i. Purines (G and A) have a double ring structure. ii. Pyrimidines (C, T and U) have a single ring structure. 4. The sequence of the RNA bases, together with the structure of the RNA molecule, determines RNA function (more in DNA unit) i. mRNA carr ...
plasmid isolation from bacteria
... In virtually all bacterial species plasmids exist. These accessory genetic elements typically account for only a small fraction of a bacterial genome corresponding roughly to a range between 1 and 200 kb. Extremely large plasmids with sizes far beyond 200 kb are also known. Plasmids of more than 5 ...
... In virtually all bacterial species plasmids exist. These accessory genetic elements typically account for only a small fraction of a bacterial genome corresponding roughly to a range between 1 and 200 kb. Extremely large plasmids with sizes far beyond 200 kb are also known. Plasmids of more than 5 ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.