Alkaline Phosphatase
... phosphate esters, including those present in nucleic acids and nucleotides. Description: > More thermal stable than Calf Intestine Alkaline Phosphatase (CIAP, CIP). > Optimal incubation temperature is approximately 60oC, however the enzyme remains active from 20oC to 80oC. > Resistant to chemical ch ...
... phosphate esters, including those present in nucleic acids and nucleotides. Description: > More thermal stable than Calf Intestine Alkaline Phosphatase (CIAP, CIP). > Optimal incubation temperature is approximately 60oC, however the enzyme remains active from 20oC to 80oC. > Resistant to chemical ch ...
GeneticEnginStudentNotes
... DNA Extraction DNA can be extracted from most cells by a simple chemical procedure. The cells are _______________ and the DNA is ______________ from the other cell parts. Cutting DNA Most DNA molecules are too ____________ to be analyzed, so biologists cut them into smaller fragments using _________ ...
... DNA Extraction DNA can be extracted from most cells by a simple chemical procedure. The cells are _______________ and the DNA is ______________ from the other cell parts. Cutting DNA Most DNA molecules are too ____________ to be analyzed, so biologists cut them into smaller fragments using _________ ...
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... In 2003, the Peltre –Thormann report commissioned by the World Anti-Doping Agency identified specific issues to address in order to improve the one-dimensional isoelectric focusing electrophoresis method currently being used for identifying drug doping with recombinant erythropoietin (rHuEPO). The n ...
... In 2003, the Peltre –Thormann report commissioned by the World Anti-Doping Agency identified specific issues to address in order to improve the one-dimensional isoelectric focusing electrophoresis method currently being used for identifying drug doping with recombinant erythropoietin (rHuEPO). The n ...
Chapter 20 Amino acids and proteins
... 2. Briefly, summarize the process of electrophoresis. 3. Given three or four amino acids, their pIs, and the pH of the buffer, determine the movement of the amino acids on an electrophoresis gel. 20.4 formation of peptides 1. Draw the structure of a dipetide from the zwitterions of two or more amino ...
... 2. Briefly, summarize the process of electrophoresis. 3. Given three or four amino acids, their pIs, and the pH of the buffer, determine the movement of the amino acids on an electrophoresis gel. 20.4 formation of peptides 1. Draw the structure of a dipetide from the zwitterions of two or more amino ...
Macromolecule Reading Guide, Part 2
... You will be reading pages 56-62 and 65-66 in the Living World and completing questions in your notebook to serve as the backbone for your notes. 3.7 Proteins ...
... You will be reading pages 56-62 and 65-66 in the Living World and completing questions in your notebook to serve as the backbone for your notes. 3.7 Proteins ...
DNA Technology and its Applications
... to change the information it contains. By changing this information, genetic engineering changes the type or amount of proteins an organism is capable of producing, thus enabling it to make new substances or perform new functions. ...
... to change the information it contains. By changing this information, genetic engineering changes the type or amount of proteins an organism is capable of producing, thus enabling it to make new substances or perform new functions. ...
Sample Preparation Guidelines for 2
... These reagents include thiols used as reducing agents and polyamines used as IPG buffers (ampholytes). Samples must also be free of ionic contaminants that hamper effective isoelectric focusing of the proteins during the first dimension. Acceptable lysis buffers and sample contaminants to avoid are ...
... These reagents include thiols used as reducing agents and polyamines used as IPG buffers (ampholytes). Samples must also be free of ionic contaminants that hamper effective isoelectric focusing of the proteins during the first dimension. Acceptable lysis buffers and sample contaminants to avoid are ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.