Application of Molecular Biotechnologies to Remediation
... Different G-C contents render different mobility in DNA-denaturing gel which is prepared to have a concentration gradient of denaturant. Probably most widely applied method for community characterization. Limitation ...
... Different G-C contents render different mobility in DNA-denaturing gel which is prepared to have a concentration gradient of denaturant. Probably most widely applied method for community characterization. Limitation ...
9/19/14 Notes on Macromolecules (powerpoint)
... • The RNA world hypothesis proposes that selfreplicating ribonucleic acid (RNA) molecules were precursors to current life, which is based on deoxyribonucleic acid (DNA), RNA and proteins. It is generally accepted that current life on Earth descends from an RNA world, although RNA-based life may not ...
... • The RNA world hypothesis proposes that selfreplicating ribonucleic acid (RNA) molecules were precursors to current life, which is based on deoxyribonucleic acid (DNA), RNA and proteins. It is generally accepted that current life on Earth descends from an RNA world, although RNA-based life may not ...
Plant collection protocol
... It is compulsory that leaf material must be collected from a SINGLE plant specimen. Labelling must contain the same exact voucher name use for the herbarium specimens. For each collection enough but not too much plant material must be collected to perform at least one DNA extraction (0.3 g of dry le ...
... It is compulsory that leaf material must be collected from a SINGLE plant specimen. Labelling must contain the same exact voucher name use for the herbarium specimens. For each collection enough but not too much plant material must be collected to perform at least one DNA extraction (0.3 g of dry le ...
Protein expression, purification, and molecular cloning
... technique used to separate proteins based upon molecular weight. Polyacrylamide is a polymer that is used to separate proteins and small DNA and RNA fragments (<100bp). While polyacrylamide is nontoxic, acrylamide is very toxic, and the process by which acrylamide forms polyacrylamide is dangerous t ...
... technique used to separate proteins based upon molecular weight. Polyacrylamide is a polymer that is used to separate proteins and small DNA and RNA fragments (<100bp). While polyacrylamide is nontoxic, acrylamide is very toxic, and the process by which acrylamide forms polyacrylamide is dangerous t ...
DNA Quantification
... DNA fragments produced through amplification or through restriction enzyme digestion are separated by using electrophoresis. Either agarose and polyacrylamide gels are used which act as a selective filter so that DNA molecules having different molecular sizes are separated into specific bands as the ...
... DNA fragments produced through amplification or through restriction enzyme digestion are separated by using electrophoresis. Either agarose and polyacrylamide gels are used which act as a selective filter so that DNA molecules having different molecular sizes are separated into specific bands as the ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.