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polioslidesnarrative
polioslidesnarrative

... It was created in 1953. Later an oral vaccination became available which is still used today. It is a weakened virus. There is no cure for polio so the only way to prevent disease is via vaccination. It currently costs 8 cents/dose. • The World Health Organization plans to eradicate polio in the nex ...
Viruses - Sign In
Viruses - Sign In

... even when the juice is passed through a filter with pores so fine that not even cells can pass through, the juice still causes the disease. When you look at a small amount of the filtered juice under the light microscope, you see no evidence of cells, juice, which is capable of transferring the dise ...
The Effects of selected toothpaste on the microbial flora of the mouth
The Effects of selected toothpaste on the microbial flora of the mouth

... Sample D and Sample E and their efficacy against different bacteria species. The population from which the data was drawn involved 30 students from different departments in Godfrey Okoye University. The students were selected using a random sampling techniques. A confirmatory experiment was also car ...
Feline Leukemia
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... veterinarian. FeLV vaccines are widely available, but since not all vaccinated cats will be protected, preventing exposure remains important even for vaccinated pets. FeLV vaccines will not cause cats to receive false positive results on ELISA, IFA, or any other available FeLV tests. ...
Replication Patterns of Specific Viruses
Replication Patterns of Specific Viruses

... strand serves as a template for synthesis of the strand of RNA or DNA in question. While the sequence of a strand of RNA is in a sense equivalent to its complement, the actual “sense” of the information encoded in the virion RNA is important for understanding how the virus replicates. As noted in Ch ...
Characteristics Of Living Organisms
Characteristics Of Living Organisms

... the genes of ancient cells (the Escape Hypothesis), while others propose that viruses could have evolved from complex molecules of protein and nucleic acid at the same time as cells first appeared on Earth (the Virus-First Hypothesis). Do some research and argue for or against each. ...
Unit 1
Unit 1

... - Selective medium contain one or more agents that inhibit the growth of a certain microbe or microbes, this encourages, or selects, a certain microbe to grow - Selective media are very important in primary isolation of a specific type of microorganism from samples that contain dozens of different s ...
Study Guide Final 244 Lecture Exam
Study Guide Final 244 Lecture Exam

... 37. Know  the  biomedical  significance  of  plasmids  and  some  of  the  capabilities  they  code  for   38. Be  able  to  describe  the  different  types  of  mutations  and  their  effects  (i.e.  missense   mutation,  nonsense  mutatio ...
Acellular and Procaryotic Microbes
Acellular and Procaryotic Microbes

... Some viruses (called enveloped viruses) have an outer envelope composed of lipids and polysaccharides (Fig. 4-3). Bacterial viruses may also have a tail, sheath, and tail fibers. There are no ribosomes for protein synthesis or sites of energy production; hence, the virus must invade and take over a ...
Hemorrhagic Fever With Renal Syndrome and Its History in Iran
Hemorrhagic Fever With Renal Syndrome and Its History in Iran

... is caused by different viruses from diverse zoonotic RNA viruses families including Flaviviridae, Bunyaviridae, Arenaviridae, and Filoviridae. 1 They are transmitted by arthropods. Rodent-associated viruses transmit directly to vertebrates by infectious excreta or secretions. There are 4 clinical sy ...
Fomites and Infection Control Presentation
Fomites and Infection Control Presentation

... hands was reduced 80 to 50%.  The occurrence of viruses in communal work areas (fomites) was reduced by more than 80% after four hours and by 70%100% after seven hours ...
II. PENGGOLONGAN MIKROBA Taksonomi Mikroba
II. PENGGOLONGAN MIKROBA Taksonomi Mikroba

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Chapter Web Links
Chapter Web Links

... 1. Planctomycetes - phylum terdiri dari bakteri dengan sifat tidak biasa, termasuk dinding sel yang tidak mempunyai peptidoglika dan sel dengan nukleoid yang diselimuti membran; membelah dengan bertunas dan membentuk tonjolan (appendages) yang disebut stalks 2. Chlamydiae - phylum terdiri dari patog ...
Indicator Microorganisms
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... Bacteroides spp. and Bifidobacteria spp.: ...
Anti-‐Microbial Resistance
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Recommended Procedures for the Extraction of RNA

... with wooden shafts (may contain PCR inhibitors) Small birds make it difficult to collect enough material for efficient extraction Cloacal samples typically recommended for waterfowl TR/OP swabs are acceptable for the surveillance of Asian H5N1 in waterfowl and wild birds RRT-PCR not recommended for ...
GENERAL BACTERIOLOGY 1. Bacterial cell
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... Motility is clearly important to many bacteria and probably serves mainly to place the cell in environments favourable to growth and free from noxious influences. In some cases possession of flagella is thought to contribute to the pathogenesis of disease. Fimbriae and pili Many bacteria possess fil ...
Henry Potosnak
Henry Potosnak

... consist of an outer protein1 encasing genetic material. Their four genetic structure configurations are: ssRNA, dsRNA, ssDNA, or dsDNA. The “ss” or “ds” indicates whether the DNA/RNA is single-stranded or double-stranded. Bacteriophages have some of the largest populations of organisms on Earth. It ...
Pathogen recognition in the innate immune response
Pathogen recognition in the innate immune response

... through somatic rearrangement of antigen receptor genes. Bcells produce pathogen-specific antibodies to neutralize toxins produced by pathogens, whereas T-cells provide the cytokine milieu to clear pathogen-infected cells through their cytotoxic effects or via signals to B-cells [5]. The mechanisms ...
disinfectants - Ark Veterinary Centre
disinfectants - Ark Veterinary Centre

... What disinfectants are safe to use for cats? Particular care must be taken in the choice of a disinfectant for use in a home with cats. Cats are generally fastidious creatures and their washing habits will leave them open to ingesting anything they get on their bodies. Cats have low levels of the li ...
Biowarfare - Anil Aggrawal`s Websites
Biowarfare - Anil Aggrawal`s Websites

... Mortality rate of V. major was about 30-35% whereas in case of V. minor it was about 1% [4].In about 65-85% of survivors, V. major lead to prolonged impediments such as scar on face. There were other complications also but was seen in only 2-5% cases which included blindness due to corneal ulceratio ...
Chapter 13
Chapter 13

... • Viruses must be grown in living cells • Bacteriophages are grown in bacteria • Bacteriophages form plaques, which are clearings on a lawn of bacteria on the surface of agar • Each plaque corresponds to a single virus; can be ...
Federal Agency for Social Development
Federal Agency for Social Development

... In microbiology the binominal system of nomenclature is accepted where each species has a generic and a specific name. The generic name is written with a capital letter, and the specific name — with a small letter. For example, the golden pus coccus is called Staphylococcus aureus; the anthrax bacil ...
Indicator Microorganisms
Indicator Microorganisms

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Catalogue Code: BA0046 Pack Size: 100 assays
Catalogue Code: BA0046 Pack Size: 100 assays

... bacteria to classify them on their ability to break down tryptophan to indole. The Assay Genie ChromaDazzle Indole assay kit is based on a modified version of Ehlrich’s and Kovac’s reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is d ...
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Virus quantification



Virus quantification involves counting the number of viruses in a specific volume to determine the virus concentration. It is utilized in both research and development (R&D) in commercial and academic laboratories as well as production situations where the quantity of virus at various steps is an important variable. For example, the production of viral vaccines, recombinant proteins using viral vectors and viral antigens all require virus quantification to continually adapt and monitor the process in order to optimize production yields and respond to ever changing demands and applications. Examples of specific instances where known viruses need to be quantified include clone screening, multiplicity of infection (MOI) optimization and adaptation of methods to cell culture. This page discusses various techniques currently used to quantify viruses in liquid samples. These methods are separated into two categories, traditional vs. modern methods. Traditional methods are industry-standard methods that have been used for decades but are generally slow and labor-intensive. Modern methods are relatively new commercially available products and kits that greatly reduce quantification time. This is not meant to be an exhaustive review of all potential methods, but rather a representative cross-section of traditional methods and new, commercially available methods. While other published methods may exist for virus quantification, non-commercial methods are not discussed here.
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