
Vaccine Handout - Shoreland, Inc
... Document is provided for sample purposes only. Content is not updated and should not be used for traveler counseling. ...
... Document is provided for sample purposes only. Content is not updated and should not be used for traveler counseling. ...
Phase1Prac-Microbio
... Molecular Tests: PCR [no antibiotic sensitivity testing] Chlamydia Trachomatis – Appearance: No Gram Stain [structurally Gram negative]; Obligate Intracellular bacterium Cultures: Gold standard for diagnosis but requires special techniques and a cell culture [as it is intracellular] Sensitivity is a ...
... Molecular Tests: PCR [no antibiotic sensitivity testing] Chlamydia Trachomatis – Appearance: No Gram Stain [structurally Gram negative]; Obligate Intracellular bacterium Cultures: Gold standard for diagnosis but requires special techniques and a cell culture [as it is intracellular] Sensitivity is a ...
Virology Questions DIRECTIONS: Each question below contains five
... 15. The most sensitive test for the diagnosis of herpes simplex (HSV) meningitis in a newborn infant is a. HSV IgG antibody b. HSV polymerase chain reaction (PCR) c. HSV culture d. Tzanck smear e. Cerebrospinal fluid (CSF) protein analysis 16. Acute hemorrhagic conjunctivitis (AHC) is a contagious o ...
... 15. The most sensitive test for the diagnosis of herpes simplex (HSV) meningitis in a newborn infant is a. HSV IgG antibody b. HSV polymerase chain reaction (PCR) c. HSV culture d. Tzanck smear e. Cerebrospinal fluid (CSF) protein analysis 16. Acute hemorrhagic conjunctivitis (AHC) is a contagious o ...
B1 1 Keeping Healthy Questions and Answers
... Autism is a brain disorder that can result in behavioural problems. In 1998, Dr Andrew Wakefield published a report in a medical journal. Dr Wakefield and his colleagues had carried out tests on 12 autistic children. Dr Wakefield and his colleagues claimed to have found a possible link between the M ...
... Autism is a brain disorder that can result in behavioural problems. In 1998, Dr Andrew Wakefield published a report in a medical journal. Dr Wakefield and his colleagues had carried out tests on 12 autistic children. Dr Wakefield and his colleagues claimed to have found a possible link between the M ...
Synergic effect of the use of anthrax and dental microbial plaque
... objective of obtaining the synergetic effect. - Is possible to rise the effects of anthrax toxins with simple addiction of cytokines (IL-1, TNF…), chemocynes (MIP, IP-10, MCP-1...) and growth factors ( ERGF…), LPS at concentration greater than 100 ng/mL, ON, PGs etc… - In my experience with dental p ...
... objective of obtaining the synergetic effect. - Is possible to rise the effects of anthrax toxins with simple addiction of cytokines (IL-1, TNF…), chemocynes (MIP, IP-10, MCP-1...) and growth factors ( ERGF…), LPS at concentration greater than 100 ng/mL, ON, PGs etc… - In my experience with dental p ...
Fowlpox virus vaccines for HIV and SHIV clinical and pre
... (lanes marked 1) is larger than the product from FPV with no insertion at that site (lanes marked M3) demonstrating the presence of additional material of the predicted size at each location. No evidence of non-recombinant FPV (as would be the case in the event of incomplete plaque purification) or ...
... (lanes marked 1) is larger than the product from FPV with no insertion at that site (lanes marked M3) demonstrating the presence of additional material of the predicted size at each location. No evidence of non-recombinant FPV (as would be the case in the event of incomplete plaque purification) or ...
Germs and Disease - International Scientific Forum on Home Hygiene
... • Numerous fungi have been found to produce volatile organic compounds (VOC) and it has been suggested that these affect the health of persons living in mouldy houses. Symptoms include headache, eye, nose and throat irritation and fatigue • VOC’s have been implicated in health issues relating to sic ...
... • Numerous fungi have been found to produce volatile organic compounds (VOC) and it has been suggested that these affect the health of persons living in mouldy houses. Symptoms include headache, eye, nose and throat irritation and fatigue • VOC’s have been implicated in health issues relating to sic ...
CDC-and-friends-going-for-the-Polio
... bowel commensal. Dr Loyd Aycock, in 1942 said “ “…frank disease among those exposed to the virus is not only greatly limited but exhibits selectivities which indicate that some added circumstance enters into the determination of whether clinical or subclinical disease results upon exposure to the vi ...
... bowel commensal. Dr Loyd Aycock, in 1942 said “ “…frank disease among those exposed to the virus is not only greatly limited but exhibits selectivities which indicate that some added circumstance enters into the determination of whether clinical or subclinical disease results upon exposure to the vi ...
Viruses and Prokaryotes Chapter 18
... likely to be living. These are just proteins that cause other proteins to act incorrectly. ...
... likely to be living. These are just proteins that cause other proteins to act incorrectly. ...
Monitoring Reactions by TLC The fastest and most commonly used
... Monitoring Reactions by TLC The fastest and most commonly used method to follow the course of an organic reaction is by thin layer chromatography (TLC). If performed properly, one can use this simple technique to (1) determine the presence of starting material in the reaction (2) detect and monitor ...
... Monitoring Reactions by TLC The fastest and most commonly used method to follow the course of an organic reaction is by thin layer chromatography (TLC). If performed properly, one can use this simple technique to (1) determine the presence of starting material in the reaction (2) detect and monitor ...
Laboratory 1
... achieved by diluting a sample containing the organism until single cells are separated from each other. The dilution can be done by serial dilution in liquid media, by spreading the sample very thinly over the surface of an agar plate (streaking) or a combination of both. Once the cell is isolated f ...
... achieved by diluting a sample containing the organism until single cells are separated from each other. The dilution can be done by serial dilution in liquid media, by spreading the sample very thinly over the surface of an agar plate (streaking) or a combination of both. Once the cell is isolated f ...
Agrobacterium rhizogenes GALLS Protein and Crown Galls
... Agrobacterium rhizogenes Causes Hairy Root Disease; A. tumefaciens Causes Unorganized Tumors (Crown Galls) ...
... Agrobacterium rhizogenes Causes Hairy Root Disease; A. tumefaciens Causes Unorganized Tumors (Crown Galls) ...
Immunoglobulin Reactivity to Pneumococcal Serotypes
... The seven types: 4, 6B, 9V, 14, 18C, 19F and 23F account for 86% of bacteremia, 83% of meningitis, and 65% of acute otitis media among children less than six years of age in the United States. These seven serotypes are responsible for 83% of invasive pneumococcal disease in children <4 years old ...
... The seven types: 4, 6B, 9V, 14, 18C, 19F and 23F account for 86% of bacteremia, 83% of meningitis, and 65% of acute otitis media among children less than six years of age in the United States. These seven serotypes are responsible for 83% of invasive pneumococcal disease in children <4 years old ...
Nitrogen Cycle - Oregon State University
... Acetylene Reduction Results Insignificant ethylene detected in all samples * Nitrogenase activity could not be confirmed in Shore Pine root samples ...
... Acetylene Reduction Results Insignificant ethylene detected in all samples * Nitrogenase activity could not be confirmed in Shore Pine root samples ...
Rabies*what is it???
... Domesticated animals make up less than 10% of reported rabies cases. Rabies virus is present not in the blood stream but in the nervous tissue . Hawaii, England, Australia, Sweden and Japan are rabies free. ...
... Domesticated animals make up less than 10% of reported rabies cases. Rabies virus is present not in the blood stream but in the nervous tissue . Hawaii, England, Australia, Sweden and Japan are rabies free. ...
Fundamental principles of immunization
... A single injection of toxoid or one of its derivatives which may sometimes be used, is seldom sufficient to produce measurable immunity, but will educate the body to respond quickly and efficiently to a subsequent stimulus. The antitoxin produced appears in the blood serum and is measurable. In this ...
... A single injection of toxoid or one of its derivatives which may sometimes be used, is seldom sufficient to produce measurable immunity, but will educate the body to respond quickly and efficiently to a subsequent stimulus. The antitoxin produced appears in the blood serum and is measurable. In this ...
Early Microbiology
... of Salmonella typhimurium can carry DNA from one bacterium to another and reported on transduction (transfer of genetic information by viruses) in 1952. Alfred Hershey and Martha Chase suggested in 1952 that only DNA is required for viral replication, after using radioactive isotopes to track protei ...
... of Salmonella typhimurium can carry DNA from one bacterium to another and reported on transduction (transfer of genetic information by viruses) in 1952. Alfred Hershey and Martha Chase suggested in 1952 that only DNA is required for viral replication, after using radioactive isotopes to track protei ...
Exam # 4 Pharmacy Tech Program
... • ______ is a noncontagious, itchy rash that often occurs in the creases of the arms, legs and neck. It is often associated with allergies. a. hay fever b. allergic rhinitis c. allergic dermatitis d. urticaria answer: c ...
... • ______ is a noncontagious, itchy rash that often occurs in the creases of the arms, legs and neck. It is often associated with allergies. a. hay fever b. allergic rhinitis c. allergic dermatitis d. urticaria answer: c ...
Viral evolution and the emergence of SARS
... populations of DNA viruses are highly variable, which hints at higher mutation rates; Sanz et al. 1999). On average, RNA polymerases produce almost one error in each replication cycle (Drake et al. 1998; Malpica et al. 2002), thus when populations of RNA viruses are large, they will produce a myriad ...
... populations of DNA viruses are highly variable, which hints at higher mutation rates; Sanz et al. 1999). On average, RNA polymerases produce almost one error in each replication cycle (Drake et al. 1998; Malpica et al. 2002), thus when populations of RNA viruses are large, they will produce a myriad ...
Interpretation of Mastitis Milk Culture Results
... numerous levels and there is no predominant organism and no obvious pathogen, the milk sample is likely contaminated upon sampling and will be reported as so. If a pathogen has been identified in addition to three or more dissimilar colony types, the reported results will include the pathogen in add ...
... numerous levels and there is no predominant organism and no obvious pathogen, the milk sample is likely contaminated upon sampling and will be reported as so. If a pathogen has been identified in addition to three or more dissimilar colony types, the reported results will include the pathogen in add ...
Medical Microbiology short answer questions
... 2. Differentiation: wash with acid-ethanol 3. Counterstaining with methylene-blue (wash with tap water after each step) 69. Describe the principle of acid fast staining procedures! Mycobacteria contain a high amount of special lipids called mycolic acids. They can be stained by hot carbol-fuchsin, b ...
... 2. Differentiation: wash with acid-ethanol 3. Counterstaining with methylene-blue (wash with tap water after each step) 69. Describe the principle of acid fast staining procedures! Mycobacteria contain a high amount of special lipids called mycolic acids. They can be stained by hot carbol-fuchsin, b ...
Hostparasite coevolution: genetic variation in a virus
... crossed all virus isolates into the same fly genetic background. Using the double balancer line SM2 ⁄ Pm; TM3 ⁄ Sb; spaPol, which has an isogenic IS4 X chromosome, we substituted the 2nd and 3rd wildtype chromosomes with the isogenic line w1118 (described by Parks et al., 2004). For the first cross, ...
... crossed all virus isolates into the same fly genetic background. Using the double balancer line SM2 ⁄ Pm; TM3 ⁄ Sb; spaPol, which has an isogenic IS4 X chromosome, we substituted the 2nd and 3rd wildtype chromosomes with the isogenic line w1118 (described by Parks et al., 2004). For the first cross, ...
Abstract book - Belgian Society for Microbiology
... into a versatile cloning system. In this setting, a single in vitro recombination reaction is sufficient to transfer any viral ORF from the collection into any expression vector and perform functional analysis of viral proteins using standardized methods. Then, large-scale mapping of virus-host prot ...
... into a versatile cloning system. In this setting, a single in vitro recombination reaction is sufficient to transfer any viral ORF from the collection into any expression vector and perform functional analysis of viral proteins using standardized methods. Then, large-scale mapping of virus-host prot ...
Virus quantification

Virus quantification involves counting the number of viruses in a specific volume to determine the virus concentration. It is utilized in both research and development (R&D) in commercial and academic laboratories as well as production situations where the quantity of virus at various steps is an important variable. For example, the production of viral vaccines, recombinant proteins using viral vectors and viral antigens all require virus quantification to continually adapt and monitor the process in order to optimize production yields and respond to ever changing demands and applications. Examples of specific instances where known viruses need to be quantified include clone screening, multiplicity of infection (MOI) optimization and adaptation of methods to cell culture. This page discusses various techniques currently used to quantify viruses in liquid samples. These methods are separated into two categories, traditional vs. modern methods. Traditional methods are industry-standard methods that have been used for decades but are generally slow and labor-intensive. Modern methods are relatively new commercially available products and kits that greatly reduce quantification time. This is not meant to be an exhaustive review of all potential methods, but rather a representative cross-section of traditional methods and new, commercially available methods. While other published methods may exist for virus quantification, non-commercial methods are not discussed here.