Exam questions to microbiology, virology and immunology course
... Medical microbiology and subject of its studying. Simple and complex methods of staining. Microscopy of native and stained smears. Discovery of L.Pasteur and its role in development of medicine. I.I.Mechnicov and his theory about resistance to infectious disease. D.I.Ivanovsky’s research as an impor ...
... Medical microbiology and subject of its studying. Simple and complex methods of staining. Microscopy of native and stained smears. Discovery of L.Pasteur and its role in development of medicine. I.I.Mechnicov and his theory about resistance to infectious disease. D.I.Ivanovsky’s research as an impor ...
Microbiology Demystified
... Each chapter follows a time-tested formula that explains topics in an easyto-read style. You can then compare your knowledge with what you’re expected to know by taking chapter tests and the final exam. There is little room for you to go adrift. ...
... Each chapter follows a time-tested formula that explains topics in an easyto-read style. You can then compare your knowledge with what you’re expected to know by taking chapter tests and the final exam. There is little room for you to go adrift. ...
Microbiology Demystified
... Bacteria are one of the most common microbes that you encounter. Some bacteria cause disease and other bacteria help you live by aiding in digestion. There are many different kinds of bacteria; however, all bacteria can be grouped into four divisions based on the characteristics of their cell walls. ...
... Bacteria are one of the most common microbes that you encounter. Some bacteria cause disease and other bacteria help you live by aiding in digestion. There are many different kinds of bacteria; however, all bacteria can be grouped into four divisions based on the characteristics of their cell walls. ...
chapter 1 - UTMB Health SHARED Home
... albopictus. Analysis of the effect of the mutations at E2-211 on CHIKV replication in cell culture and on CHIKV binding to the brush border membrane proteins of Ae.albopictus midgut cells, indicated that different residues at E2-211 might differentially affect the ability of CHIKV to interact with s ...
... albopictus. Analysis of the effect of the mutations at E2-211 on CHIKV replication in cell culture and on CHIKV binding to the brush border membrane proteins of Ae.albopictus midgut cells, indicated that different residues at E2-211 might differentially affect the ability of CHIKV to interact with s ...
The viral killer system in yeast: from molecular biology to application
... remain isolated in their host and might explain why ScVM killer virions remained distinct and have not been found as recombinants or hybrids [19]. L-A itself is an autonomously replicating mycovirus neither conferring a detectable phenotype upon its host (yeast) cell, nor requiring the presence of a ...
... remain isolated in their host and might explain why ScVM killer virions remained distinct and have not been found as recombinants or hybrids [19]. L-A itself is an autonomously replicating mycovirus neither conferring a detectable phenotype upon its host (yeast) cell, nor requiring the presence of a ...
18.1 Studying Viruses and Prokaryotes
... shingles later in adulthood • Fever is higher • Immune system weakens • Pneumonia may occur ...
... shingles later in adulthood • Fever is higher • Immune system weakens • Pneumonia may occur ...
Laboratory diagnosis of sexually transmitted infections, including
... partners, and emphasis on the importance of same-visit HIV testing. The flowcharts should be based on local etiological and antimicrobial susceptibility data provided through periodic laboratory-based surveys. In general, laboratory tests are not undertaken for most STI patients who receive syndromi ...
... partners, and emphasis on the importance of same-visit HIV testing. The flowcharts should be based on local etiological and antimicrobial susceptibility data provided through periodic laboratory-based surveys. In general, laboratory tests are not undertaken for most STI patients who receive syndromi ...
univERsity oF copEnhAGEn
... Oxidative/Fermentative activity: Semi-solid Hugh-Leifson (H-L) agar medium contains glucose as a C-source for oxidative or fermentative activity. Aerobic tubes without vaseline: Oxidative activity can occur thoughout the tube, supported by unlimited O2 diffusion from the agar surface. Anaerobic tube ...
... Oxidative/Fermentative activity: Semi-solid Hugh-Leifson (H-L) agar medium contains glucose as a C-source for oxidative or fermentative activity. Aerobic tubes without vaseline: Oxidative activity can occur thoughout the tube, supported by unlimited O2 diffusion from the agar surface. Anaerobic tube ...
Output Interpretation - UCSF Viral Diagnostics and Discovery Center
... aligned using SNAP to all nucleotide sequences in the NCBI nt collection, enabling identification of bacteria, fungi, parasites, and viruses. Unclassified reads and contigs generated from de novo assembly are then aligned to a viral protein database using RAPSearch for pathogen discovery of divergen ...
... aligned using SNAP to all nucleotide sequences in the NCBI nt collection, enabling identification of bacteria, fungi, parasites, and viruses. Unclassified reads and contigs generated from de novo assembly are then aligned to a viral protein database using RAPSearch for pathogen discovery of divergen ...
Medical Microbiology Core Competencies and Knowledge
... 1. Compare and contrast prokaryotic and eukaryotic cells, particularly with respect to nuclear membranes, DNA structure, ribosomes, and cell walls. 2. Describe the morphology and arrangement of bacterial cells using acceptable scientific terms (cocci, bacilli, etc). 3. Explain the use of the Gram an ...
... 1. Compare and contrast prokaryotic and eukaryotic cells, particularly with respect to nuclear membranes, DNA structure, ribosomes, and cell walls. 2. Describe the morphology and arrangement of bacterial cells using acceptable scientific terms (cocci, bacilli, etc). 3. Explain the use of the Gram an ...
AMMI Canada – CACMID Annual Conference
... are tested to see if they will ‘seed’ the conversion process. Cycles of shaking and incubation will cause infected CSF, containing PrPd, to generate greatly increased amounts of rPrPd facilitating subsequent detection by traditional methods. RESULTS: In a retrospective study, CSF samples from 43 pat ...
... are tested to see if they will ‘seed’ the conversion process. Cycles of shaking and incubation will cause infected CSF, containing PrPd, to generate greatly increased amounts of rPrPd facilitating subsequent detection by traditional methods. RESULTS: In a retrospective study, CSF samples from 43 pat ...
Bacteriophage
... bacteria infected that is not part of a usual phage cycle. Changes can often involve the external membrane of the cell by making it impervious to other phages or even by increasing the pathogenic capability of the bacteria for a host. ...
... bacteria infected that is not part of a usual phage cycle. Changes can often involve the external membrane of the cell by making it impervious to other phages or even by increasing the pathogenic capability of the bacteria for a host. ...
Ammonia as an In Situ Sanitizer: Influence of Virus Genome Type on
... virus stocks for the experiments. New stocks were produced before each set of experiments. Virus titers were determined by most probable number analysis from 5 ⫻ 100 l of samples on 96-well plates (Greiner BioOne, Frickenhausen, Germany), as described by Bosshard et al. (43). Briefly, the DMEM/MEM ...
... virus stocks for the experiments. New stocks were produced before each set of experiments. Virus titers were determined by most probable number analysis from 5 ⫻ 100 l of samples on 96-well plates (Greiner BioOne, Frickenhausen, Germany), as described by Bosshard et al. (43). Briefly, the DMEM/MEM ...
CASE 1 INTRODUCTION A 53-year-old male farmer presents for
... The differential diagnosis of a patient (farmer) with fever, adenopathy, and black eschar include other cutaneous lesions such as furuncles (staphylococci), ecthyma gangrenosum (Pseudomonas aeruginosa), and spider bites. However, none of these etiologies are known to cause eschar formation with surr ...
... The differential diagnosis of a patient (farmer) with fever, adenopathy, and black eschar include other cutaneous lesions such as furuncles (staphylococci), ecthyma gangrenosum (Pseudomonas aeruginosa), and spider bites. However, none of these etiologies are known to cause eschar formation with surr ...
Features of biofilms
... approaching a surface, the signal molecule concentration increases in the area between the micro-organism and the surface, due to limited diffusion. The ...
... approaching a surface, the signal molecule concentration increases in the area between the micro-organism and the surface, due to limited diffusion. The ...
Dialysis Guide_scienova
... higher concentration to lower concentration. Selectivity of dialysis is determined by pore size of semipermeable membranes. The end point of dialysis is the concentration equilibrium. Dialysis is ...
... higher concentration to lower concentration. Selectivity of dialysis is determined by pore size of semipermeable membranes. The end point of dialysis is the concentration equilibrium. Dialysis is ...
Advances in Rapid Detection Methods for Foodborne Pathogens
... methods have been developed to amplify nucleic acids under isothermal conditions. These methods include loopmediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASBA), rolling circle amplification (RCA), and strand displacement amplification (SDA). Isothermal amplifi ...
... methods have been developed to amplify nucleic acids under isothermal conditions. These methods include loopmediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASBA), rolling circle amplification (RCA), and strand displacement amplification (SDA). Isothermal amplifi ...
Dissertation zur Erlangung des Doktorgrades der Fakultät für Chemie und Pharmazie
... sequence homology, have been identified. Although the other serotypes have attracted increasing attention during recent years, AAV type 2 is the most prominent serotype for gene therapy, being the first isolated, cloned, and best characterized. Since all following descriptions will refer to AAV type ...
... sequence homology, have been identified. Although the other serotypes have attracted increasing attention during recent years, AAV type 2 is the most prominent serotype for gene therapy, being the first isolated, cloned, and best characterized. Since all following descriptions will refer to AAV type ...
Isolation and expression of recombinant antibody
... et al., 2000). Antibody fragments can be readily expressed in Escherichia coli allowing low cost production and purification, an important advantage for many applications including proteomics (Hayhurst and Georgiou, 2001). Phage display is routinely used for the isolation of antibody fragments to a ...
... et al., 2000). Antibody fragments can be readily expressed in Escherichia coli allowing low cost production and purification, an important advantage for many applications including proteomics (Hayhurst and Georgiou, 2001). Phage display is routinely used for the isolation of antibody fragments to a ...
ABSTRACT Title of Document:
... adaptation. The generation of an influenza pandemic requires interspecies transmission of a novel strain, which can adapt to its new host through either reassortment or point mutations. Given that two previous pandemics were the result of reassortment between low pathogenic avian viruses and human s ...
... adaptation. The generation of an influenza pandemic requires interspecies transmission of a novel strain, which can adapt to its new host through either reassortment or point mutations. Given that two previous pandemics were the result of reassortment between low pathogenic avian viruses and human s ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... decarded to concentred protein in the solution obtained by using precipitation in cold absolute ethanol. Hemagglutination test method Hemagglutination assay was done according to the instructions of Hanne and Finkelstein’s [16]. Sample dilutions were made on ½ concentration in microplate V where eac ...
... decarded to concentred protein in the solution obtained by using precipitation in cold absolute ethanol. Hemagglutination test method Hemagglutination assay was done according to the instructions of Hanne and Finkelstein’s [16]. Sample dilutions were made on ½ concentration in microplate V where eac ...
18.1 Studying Viruses and Prokaryotes
... shingles later in adulthood • Fever is higher • Immune system weakens • Pneumonia may occur ...
... shingles later in adulthood • Fever is higher • Immune system weakens • Pneumonia may occur ...
SYNOPSIS
... 1. Microscopic observation of bacteria in unstained conditions. 2. Microscopic observation of bacteria in stained preparations: Loeffler's methylene blue and Gram stain. 3. Microscopic observation of bacteria in stained preparations: Acid-fast stain (Ziehl-Neelsen stain) and staining procedures for ...
... 1. Microscopic observation of bacteria in unstained conditions. 2. Microscopic observation of bacteria in stained preparations: Loeffler's methylene blue and Gram stain. 3. Microscopic observation of bacteria in stained preparations: Acid-fast stain (Ziehl-Neelsen stain) and staining procedures for ...
Light Forge: A Microfluidic High Throughput Platform for Rapid and
... Recent breakthroughs in the field of cryo-electron microscopy provide new prospects for determination of the structures of a variety of macromolecular assemblies and small dynamic protein complexes that are not amenable to analysis by X-ray crystallography or NMR spectroscopy. In addition, advances ...
... Recent breakthroughs in the field of cryo-electron microscopy provide new prospects for determination of the structures of a variety of macromolecular assemblies and small dynamic protein complexes that are not amenable to analysis by X-ray crystallography or NMR spectroscopy. In addition, advances ...
Sampling feasibility study
... Although the EU regulatory framework only covers genetically modified micro-organisms (GMMs), the scope of the Belgian regional legislations has been extended to genetically modified organisms and pathogenic organisms for humans, animal and plants. The three Regions (Flemish Region, Walloon Region a ...
... Although the EU regulatory framework only covers genetically modified micro-organisms (GMMs), the scope of the Belgian regional legislations has been extended to genetically modified organisms and pathogenic organisms for humans, animal and plants. The three Regions (Flemish Region, Walloon Region a ...
Virus quantification
Virus quantification involves counting the number of viruses in a specific volume to determine the virus concentration. It is utilized in both research and development (R&D) in commercial and academic laboratories as well as production situations where the quantity of virus at various steps is an important variable. For example, the production of viral vaccines, recombinant proteins using viral vectors and viral antigens all require virus quantification to continually adapt and monitor the process in order to optimize production yields and respond to ever changing demands and applications. Examples of specific instances where known viruses need to be quantified include clone screening, multiplicity of infection (MOI) optimization and adaptation of methods to cell culture. This page discusses various techniques currently used to quantify viruses in liquid samples. These methods are separated into two categories, traditional vs. modern methods. Traditional methods are industry-standard methods that have been used for decades but are generally slow and labor-intensive. Modern methods are relatively new commercially available products and kits that greatly reduce quantification time. This is not meant to be an exhaustive review of all potential methods, but rather a representative cross-section of traditional methods and new, commercially available methods. While other published methods may exist for virus quantification, non-commercial methods are not discussed here.