Bacterial and fungal infections
... pneumonia) can render negative blood cultures 60. The occurrence of these fastidious and slow-growing pathogens will cause a serious delay in the identification process, which can lead to prolonged and inadequate empirical antimicrobial therapy. Another potential interfering factor resulting in nega ...
... pneumonia) can render negative blood cultures 60. The occurrence of these fastidious and slow-growing pathogens will cause a serious delay in the identification process, which can lead to prolonged and inadequate empirical antimicrobial therapy. Another potential interfering factor resulting in nega ...
The characterization of inner core protein VP6 of African Horsesickness Virus
... In 1898 researchers identified infectious agents that were smaller than the smallest known bacteria. Viruses are obligate intracellular parasites that use the machinery of the host cell to replicate. They consist of a DNA or RNA genome surrounded by a protein capsid, which may be surrounded by an en ...
... In 1898 researchers identified infectious agents that were smaller than the smallest known bacteria. Viruses are obligate intracellular parasites that use the machinery of the host cell to replicate. They consist of a DNA or RNA genome surrounded by a protein capsid, which may be surrounded by an en ...
A plAtform for Anti-biofilm AssAys
... gratitude towards the many people who have helped me one way or another over the years. First of all I would like to thank professor Pia Vuorela for taking me on-board as a graduate student, for your endless encouragement and support along the way. Then an enormous gratitude goes to the best supervi ...
... gratitude towards the many people who have helped me one way or another over the years. First of all I would like to thank professor Pia Vuorela for taking me on-board as a graduate student, for your endless encouragement and support along the way. Then an enormous gratitude goes to the best supervi ...
RESPIRATORY PATHOGENS IN THOROUGHBRED FOALS UP TO ONE YEAR OF AGE
... Only EHV-4 was cultured from the nasopharyngeal swabs of nine foals when they were 5 to 6 months of age and from one foal two months later. A wide variety of bacteria and fungi were cultured and it was established that coagulase-negative staphylococci, viridans streptococci, Moraxella spp. and Flavo ...
... Only EHV-4 was cultured from the nasopharyngeal swabs of nine foals when they were 5 to 6 months of age and from one foal two months later. A wide variety of bacteria and fungi were cultured and it was established that coagulase-negative staphylococci, viridans streptococci, Moraxella spp. and Flavo ...
Vinnitsa National Pirogov Memorial Medical University Biological
... After I had finished to study chemistry ______________ years/months passed. The students who studied chemistry at their countries we ask to read attentively the question given below. While answering the questions you should be attentive and don not be in a hurry. First of all try to remember all you ...
... After I had finished to study chemistry ______________ years/months passed. The students who studied chemistry at their countries we ask to read attentively the question given below. While answering the questions you should be attentive and don not be in a hurry. First of all try to remember all you ...
MOLECULAR CHARACTERIZATION OF A PORCINE PICOBIRNAVIRUS RNA- DEPENDENT RNA POLYMERASE. by
... Picobirnavirus is an unclassified dsRNA virus, which is associated with viral gastroenteritis in humans and animals. Picobirnavirus dsRNA has been detected in many cases when diagnostic PAGE screening for rotavirus dsRNA is performed. During this routine diagnosis, picobirnavirus dsRNA has been dete ...
... Picobirnavirus is an unclassified dsRNA virus, which is associated with viral gastroenteritis in humans and animals. Picobirnavirus dsRNA has been detected in many cases when diagnostic PAGE screening for rotavirus dsRNA is performed. During this routine diagnosis, picobirnavirus dsRNA has been dete ...
Culture-based Methods for Detection of Antibiotic Resistance in
... Pseudomonas, and Salmonella (CHROMagar). Many of the available selective media can have high (50–75%) “false-positive” rates (i.e., isolates that present characteristics of the target species but actually are not that species) when used to identify environmental bacteria (Peplow et al., 1999; Robert ...
... Pseudomonas, and Salmonella (CHROMagar). Many of the available selective media can have high (50–75%) “false-positive” rates (i.e., isolates that present characteristics of the target species but actually are not that species) when used to identify environmental bacteria (Peplow et al., 1999; Robert ...
Annual Conference 2017
... variants constructed using error-prone PCR. The cytotoxicity of N variants was tested by MTT assay. Expressed N variants showed a range of effects ranging from the expected 10-fold increase in virus yield to 10-fold inhibition of virus growth. One particular N variant, mutant38, was non-toxic, but r ...
... variants constructed using error-prone PCR. The cytotoxicity of N variants was tested by MTT assay. Expressed N variants showed a range of effects ranging from the expected 10-fold increase in virus yield to 10-fold inhibition of virus growth. One particular N variant, mutant38, was non-toxic, but r ...
PDF
... progressive methods of teaching and changes in the curriculum over the past century have led to a variety of approaches.13 In general, didactic lectures and paper examinations have been replaced in favor of problembased learning14, team-based learning15, e-based small group, simulation-based learnin ...
... progressive methods of teaching and changes in the curriculum over the past century have led to a variety of approaches.13 In general, didactic lectures and paper examinations have been replaced in favor of problembased learning14, team-based learning15, e-based small group, simulation-based learnin ...
id telt
... • xTAG® GPP is the first diagnostic to offer detection of 15 major gastrointestinal pathogens in a single test • Results within 5 hours for timely, well-informed treatment decisions and better patient care • Fast turn-around time and multiplex testing mean better use of time and human resources ...
... • xTAG® GPP is the first diagnostic to offer detection of 15 major gastrointestinal pathogens in a single test • Results within 5 hours for timely, well-informed treatment decisions and better patient care • Fast turn-around time and multiplex testing mean better use of time and human resources ...
Simple Organisms, Fungi, and Plants
... 1. physical science as the study of energy and matter (Section 1) 2. the role of physical science in the world around them (Section 1) 3. careers that rely on physical science (Section 1) 4. the steps used in the scientific method (Section 2) 5. examples of technology (Section 2) ...
... 1. physical science as the study of energy and matter (Section 1) 2. the role of physical science in the world around them (Section 1) 3. careers that rely on physical science (Section 1) 4. the steps used in the scientific method (Section 2) 5. examples of technology (Section 2) ...
DEVELOPMENT OF FLUORESCENT IN SITU HYBRIDIZATION FOR
... environments. It is known as a facultative intracellular parasite of protozoa in its natural habitat, and can also infect macrophages in humans when contaminated aerosols are inhaled. The current detection procedure for L. pneumophila is a culture based method, which is slow and may not detect viabl ...
... environments. It is known as a facultative intracellular parasite of protozoa in its natural habitat, and can also infect macrophages in humans when contaminated aerosols are inhaled. The current detection procedure for L. pneumophila is a culture based method, which is slow and may not detect viabl ...
DEVELOPMENT AND CHARACTERIZATION OF A TRANSGENIC
... strain. As a result of vaccine-associated disease, there is some impetus to construct, by genetic engineering, vaccine strains which do not revert to neurovirulence. A significant obstacle to the development of new poliovirus vaccines is the cost and availability of the large number of monkeys that ...
... strain. As a result of vaccine-associated disease, there is some impetus to construct, by genetic engineering, vaccine strains which do not revert to neurovirulence. A significant obstacle to the development of new poliovirus vaccines is the cost and availability of the large number of monkeys that ...
Supplemental information
... TEM samples were prepared by applying drops of stabilized nanoparticle suspension to a carbon-coated copper grid and then drying the grid, which was then measured in a JEM-2010 TEM (JEOL Ltd., Tokyo, Japan). For particle size analysis, nanoparticle size in the TEM images was compared with a nanomete ...
... TEM samples were prepared by applying drops of stabilized nanoparticle suspension to a carbon-coated copper grid and then drying the grid, which was then measured in a JEM-2010 TEM (JEOL Ltd., Tokyo, Japan). For particle size analysis, nanoparticle size in the TEM images was compared with a nanomete ...
Introduction to Modern Virology
... bear in order to elucidate their subject and thus pick up some of the excitement of working in this field. Virology is a vibrant area and its study, far from being constraining, opens up a vista in which virus infections can be understood in the context of the biology of their hosts. ...
... bear in order to elucidate their subject and thus pick up some of the excitement of working in this field. Virology is a vibrant area and its study, far from being constraining, opens up a vista in which virus infections can be understood in the context of the biology of their hosts. ...
lecture26.pps
... Cholera Toxin Mechanism of Action (explanation for handout) Cholera toxin is a protein molecule comprised of a beta subunit (consisting of 5 noncovalently linked molecules) and an alpha subunit (containing 2 peptides, alpha 1 and 2) and having a molecular weight of ~84,000. The 5 beta subunit prote ...
... Cholera Toxin Mechanism of Action (explanation for handout) Cholera toxin is a protein molecule comprised of a beta subunit (consisting of 5 noncovalently linked molecules) and an alpha subunit (containing 2 peptides, alpha 1 and 2) and having a molecular weight of ~84,000. The 5 beta subunit prote ...
E self-assembled monolayers Jen-Jr Gau , Esther H. Lan
... conditions. The need for rapid detection as well as portability has led to the development of systems that couple pathogen recognition with signal transduction. Both optical and electrochemical detection of bacteria have been reported, although electrochemical methods have an advantage in that they ...
... conditions. The need for rapid detection as well as portability has led to the development of systems that couple pathogen recognition with signal transduction. Both optical and electrochemical detection of bacteria have been reported, although electrochemical methods have an advantage in that they ...
Fate and Transport of Zoonotic Bacterial, Viral, and Parasitic
... (2) the survival properties of different viruses are very variable, preventing extrapolation of findings from one virus strain to another. Sobsey and colleagues (2005) evaluated microbial reductions achieved by several swine manure management technologies. In this study, single nonaerated lagoon sys ...
... (2) the survival properties of different viruses are very variable, preventing extrapolation of findings from one virus strain to another. Sobsey and colleagues (2005) evaluated microbial reductions achieved by several swine manure management technologies. In this study, single nonaerated lagoon sys ...
Evolution of parasitism and mutualism between filamentous
... when a symbiont enters a new host either through direct contact with an unrelated, infected host, or through contact with a free symbiont in the host’s environment. Both parasites and mutualists can be horizontally transmitted. In contrast, vertical transmission is the direct intergenerational trans ...
... when a symbiont enters a new host either through direct contact with an unrelated, infected host, or through contact with a free symbiont in the host’s environment. Both parasites and mutualists can be horizontally transmitted. In contrast, vertical transmission is the direct intergenerational trans ...
UG Microbiology QP Jan 2008 Dec 2013
... QP Code: 1084 Your answers should be specific to the questions asked. Draw neat labeled diagrams wherever necessary. LONG ESSAY 2 X 10 = 20 Marks ...
... QP Code: 1084 Your answers should be specific to the questions asked. Draw neat labeled diagrams wherever necessary. LONG ESSAY 2 X 10 = 20 Marks ...
MEDICAL MICROBIOLOGY
... diversity, and the genetic determination of both the pathogenicity and virulence of microorganisms and resistance to antimicrobial substances. • To learn about the natural groups and taxonomic categories of the microbial world, as well as the methods used in classification. • To acquire a basic unde ...
... diversity, and the genetic determination of both the pathogenicity and virulence of microorganisms and resistance to antimicrobial substances. • To learn about the natural groups and taxonomic categories of the microbial world, as well as the methods used in classification. • To acquire a basic unde ...
Chapter 3 (Biology and Diseases of Mice)
... The predominant inflammatory infiltrate is compromised of _________ _____, but some ___________ are present. How is PVM diagnosed? True or False. PVM virus can be detected in tissue by RT-PCR. In immunodeficient mice, PVM must be differentiated from other pneumonias, especially those due to ________ ...
... The predominant inflammatory infiltrate is compromised of _________ _____, but some ___________ are present. How is PVM diagnosed? True or False. PVM virus can be detected in tissue by RT-PCR. In immunodeficient mice, PVM must be differentiated from other pneumonias, especially those due to ________ ...
RPI Biosafety Plan - Rensselaer Polytechnic Institute
... equipment, and facilities required to safely conduct research on infectious agents. There are four biosafety levels; the level recommended for work with a particular infectious agent depends upon many factors including its virulence and means of infection. Descriptions of each biosafety level are li ...
... equipment, and facilities required to safely conduct research on infectious agents. There are four biosafety levels; the level recommended for work with a particular infectious agent depends upon many factors including its virulence and means of infection. Descriptions of each biosafety level are li ...
biosafety guidelines risk assessment of genetically
... It is important to appreciate that the genetic modification of a microorganism can affect its ability to cause harm to human health, animal health and the environment. Therefore, the Act requires risk assessment, risk management and emergency response plans, endorsed by the Institutional Biosafety C ...
... It is important to appreciate that the genetic modification of a microorganism can affect its ability to cause harm to human health, animal health and the environment. Therefore, the Act requires risk assessment, risk management and emergency response plans, endorsed by the Institutional Biosafety C ...
Virus quantification
Virus quantification involves counting the number of viruses in a specific volume to determine the virus concentration. It is utilized in both research and development (R&D) in commercial and academic laboratories as well as production situations where the quantity of virus at various steps is an important variable. For example, the production of viral vaccines, recombinant proteins using viral vectors and viral antigens all require virus quantification to continually adapt and monitor the process in order to optimize production yields and respond to ever changing demands and applications. Examples of specific instances where known viruses need to be quantified include clone screening, multiplicity of infection (MOI) optimization and adaptation of methods to cell culture. This page discusses various techniques currently used to quantify viruses in liquid samples. These methods are separated into two categories, traditional vs. modern methods. Traditional methods are industry-standard methods that have been used for decades but are generally slow and labor-intensive. Modern methods are relatively new commercially available products and kits that greatly reduce quantification time. This is not meant to be an exhaustive review of all potential methods, but rather a representative cross-section of traditional methods and new, commercially available methods. While other published methods may exist for virus quantification, non-commercial methods are not discussed here.