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Manipulating DNA extracting and studying DNA
Manipulating DNA extracting and studying DNA

... used to make many copies of a desired gene.  Steps to PCR 1. DNA strands are separated by heating. 2. Short pieces of DNA, called primers, are attached to the DNA strands to prepare a place for DNA polymerase to start copying. 3. These copies serve as templates for more copies. ...
DNA damage and repair
DNA damage and repair

... •DNA damage refers to a chemical alteration of the DNA (e.g. G-C bp to methyl-G-C is DNA damage) •Mutation refers to a change in a base-pair (e.g. G-C bp to A-T bp is a mutation) •There are long term (inhertided) implications when DNA damage is converted to mutation ...
Answers-pg-294 - WordPress.com
Answers-pg-294 - WordPress.com

... in eukaryotic cells, efficiency as compared to bacteria, packing the process would efficiencysimply take too long in eukaryotic cells -if there were only one replication site.telomeres An alternative hypothesis is that free floating DNA - contains to the small size of the circular chromosomes ofprot ...
Biotechnological Tools and Techniques
Biotechnological Tools and Techniques

... AKA – Restriction Enzymes These enzymes cut DNA at specific sequences called recognition sites. The cuts at the recognition sites can be in the form of either blunt ends or sticky ends (with sticky ends being the more useful of the two). Restriction enzymes naturally occur in bacteria as a defense a ...
DNA PROFILING
DNA PROFILING

... A technique used by scientists to distinguish between individuals of the same species using only samples of their DNA ...
DNA consists of two strands, each of which is a linear arrangement
DNA consists of two strands, each of which is a linear arrangement

... DNA consists of two strands, each of which is a linear arrangement of nucleotides. All nucleotides of DNA contain an identical pentose sugar molecule (deoxyribose) and an identical phosphate group. Their third component, a nitrogenous base, exists in four different forms (adenine: A; guanine: G; thy ...
protein synthesis - Ms. Dooley`s Science Class
protein synthesis - Ms. Dooley`s Science Class

... PROTEIN SYNTHESIS This activity will help you become more familiar with the process of protein synthesis and will help distinguish between transcription and translation. Use your book to help review any problems. PART 1 - Transcription During transcription, the DNA double helix “unzips”. As the hydr ...
DNA Review Sheet Answers
DNA Review Sheet Answers

... DNA Polymerase then adds new complementary bases to each strand of DNA. This process is said to be semi- conservative because one half of the original DNA double helix is conserved in each of the new strands. ...
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... •Mutation refers to a change in a base-pair (e.g. G-C bp to A-T bp is a mutation) •Problems arise when DNA damage is converted to mutation ...
Biology I Formative Assessment #7
Biology I Formative Assessment #7

... C. DNA replication is important for ensuring that organisms have common ancestry. D. DNA replication is important for transmitting and conserving genetic information. SC.912.L.16.3 2. As a cell prepares to divide during the cell cycle, DNA replication must take place. During the S-phase, DNA strands ...
DNA Replication
DNA Replication

... Just before the first nuclear division, homologous chromosomes pair along their lengths, so that now for each chromosomal type there are two pairs of sister chromatids juxtaposed, making a bundle of four also called a tetrad. At the tetrad stage a remarkable process occurs: paired nonsister chromati ...
A-DNA
A-DNA

... The 2 strands are twisted about each other, coiled around a common axis, forming a righthanded double helix. The hydrophilic sugar- phosphate backbone of each chain lies on the outside of the molecule. The hydrophobic nitrogenous bases project inwards from the outer sugar-phosphate framework, perpen ...
DNA Technology
DNA Technology

... to inherited breast cancer ...
DNA - Fort Bend ISD
DNA - Fort Bend ISD

... 1. Fredrick Griffith (microbiology research with nucleic acids) 2. Oswald Avery (colleague that completed/supported Griffith’s work) 3. Alfred Hershey and Martha Chase (proved that DNA and not protein is the genetic material of life) 4. Erwin Chargaff (A-T, G-C) 5. Rosalind Franklin (x-ray pics-DNA ...
QCM2 - GIGA
QCM2 - GIGA

... A.  remove amino acids from the carboxyl end of a polypeptide chain.   B.  connect two DNA fragments together.   C.  remove terminal nucleotides from the 3'‐end of a polynucleotide chain.   D.  connect two oligopeptides together.   E.  break internal phosphodiester bonds in a single‐ or double‐stran ...
DNA fingerprint - cloudfront.net
DNA fingerprint - cloudfront.net

...  The greek letter “mu” is used to represent micro (μ)  Let’s PRACTICE   Work through the second page of the packet “DNA Fingerprinting – Practice Worksheet” ...
Name: ____________ Pd.: ______ Date: Cells cannot make
Name: ____________ Pd.: ______ Date: Cells cannot make

... of amino acids which make up proteins) 4. The double helix structure explains how DNA can be replicated, or copied, but it does not explain how a gene works. Genes are coded DNA instructions that control the production of proteins within the cell. The first step in decoding these genetic messages is ...
Name - WordPress.com
Name - WordPress.com

... In class we’ve been talking about how offspring inherit traits from their parents. We know that they inherit alleles, which are copies of genes, by receiving DNA from their parents. We also know that the DNA is located within a chromosome inside the nucleus of a gamete, or sex cell. We can actually ...
Model question Paper- Gene Technology MLAB 475
Model question Paper- Gene Technology MLAB 475

... J. degrades RNA into smaller components. K. the enzyme in humans that can start an RNA chain from scratch. ...
DNA Fingerprinting Lab
DNA Fingerprinting Lab

... ✓You would be given a sequence of DNA and told of a restriction enzyme that cuts at a particular sequence of base pairs. ✓You would then make a cut in the DNA (draw a line) to make the DNA fragments (you will be the restriction enzyme). ...
Biotechnology Powerpoint
Biotechnology Powerpoint

... That the parental rights held by the Twiggs compelled that they be granted custody of 14year-old Kimberley Mays who was switched at birth with another newborn. Chief Lawyer for Plaintiff ...
Name AP EXAM REVIEW SESSION II ASSESSMENT QUIZ Use the
Name AP EXAM REVIEW SESSION II ASSESSMENT QUIZ Use the

... 16. The discontinuous strand during DNA replication is known as the a. leading strand b. lagging strand c. promoter d. operator e. operon 17. Which of the following statements is true about the leading strand during DNA synthesis? a. Primase reads the DNA and adds DNA in short segments. b. DNA repli ...
1. True or False? A typical chromosome can contain
1. True or False? A typical chromosome can contain

... A.  remove amino acids from the carboxyl end of a polypeptide chain.   B.  connect two DNA fragments together.   C.  remove terminal nucleotides from the 3'‐end of a polynucleotide chain.   D.  connect two oligopeptides together.   E.  break internal phosphodiester bonds in a single‐ or double‐stran ...
Tilting and tiling
Tilting and tiling

... incoherent hops through low-potential regions of the stacked base pairs, for example guanines — the most easily oxidized bases — or transient delocalized domains extending over several bases. The domains are defined by the base sequence. If low-potential wellstacked sites replace the high potential ...
DNA - The Double Helix Read and HIGHLIGHT what you consider is
DNA - The Double Helix Read and HIGHLIGHT what you consider is

... the nitrogen bases pair up in a specific way. He found that n human DNA, for example, the four bases are present in these percentages: A=30.9% and T=29.4%; G=19.9% and C=19.8%. This strongly hinted towards the base pair makeup of the DNA. The rungs of the DNA ‘ladder’ are pairs of 4 types of nitroge ...
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DNA



Deoxyribonucleic acid (/diˌɒksiˌraɪbɵ.njuːˌkleɪ.ɨk ˈæsɪd/; DNA) is a molecule that carries most of the genetic instructions used in the development, functioning and reproduction of all known living organisms and many viruses. DNA is a nucleic acid; alongside proteins and carbohydrates, nucleic acids compose the three major macromolecules essential for all known forms of life. Most DNA molecules consist of two biopolymer strands coiled around each other to form a double helix. The two DNA strands are known as polynucleotides since they are composed of simpler units called nucleotides. Each nucleotide is composed of a nitrogen-containing nucleobase—either cytosine (C), guanine (G), adenine (A), or thymine (T)—as well as a monosaccharide sugar called deoxyribose and a phosphate group. The nucleotides are joined to one another in a chain by covalent bonds between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone. According to base pairing rules (A with T, and C with G), hydrogen bonds bind the nitrogenous bases of the two separate polynucleotide strands to make double-stranded DNA. The total amount of related DNA base pairs on Earth is estimated at 5.0 x 1037, and weighs 50 billion tonnes. In comparison, the total mass of the biosphere has been estimated to be as much as 4 TtC (trillion tons of carbon).DNA stores biological information. The DNA backbone is resistant to cleavage, and both strands of the double-stranded structure store the same biological information. Biological information is replicated as the two strands are separated. A significant portion of DNA (more than 98% for humans) is non-coding, meaning that these sections do not serve as patterns for protein sequences.The two strands of DNA run in opposite directions to each other and are therefore anti-parallel. Attached to each sugar is one of four types of nucleobases (informally, bases). It is the sequence of these four nucleobases along the backbone that encodes biological information. Under the genetic code, RNA strands are translated to specify the sequence of amino acids within proteins. These RNA strands are initially created using DNA strands as a template in a process called transcription.Within cells, DNA is organized into long structures called chromosomes. During cell division these chromosomes are duplicated in the process of DNA replication, providing each cell its own complete set of chromosomes. Eukaryotic organisms (animals, plants, fungi, and protists) store most of their DNA inside the cell nucleus and some of their DNA in organelles, such as mitochondria or chloroplasts. In contrast, prokaryotes (bacteria and archaea) store their DNA only in the cytoplasm. Within the chromosomes, chromatin proteins such as histones compact and organize DNA. These compact structures guide the interactions between DNA and other proteins, helping control which parts of the DNA are transcribed.First isolated by Friedrich Miescher in 1869 and with its molecular structure first identified by James Watson and Francis Crick in 1953, DNA is used by researchers as a molecular tool to explore physical laws and theories, such as the ergodic theorem and the theory of elasticity. The unique material properties of DNA have made it an attractive molecule for material scientists and engineers interested in micro- and nano-fabrication. Among notable advances in this field are DNA origami and DNA-based hybrid materials.The obsolete synonym ""desoxyribonucleic acid"" may occasionally be encountered, for example, in pre-1953 genetics.
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