lab- where`s the CAT palffy 2010-1
... Olsons had babies at the same time at the same hospital. The Jacobsons think that the babies were switched. Electrophoresis can be used to help settle the matter. DNA profiling works in the following manner. 1.Cells are taken from an individual. The cells are broken open and the DNA is extracted. 2. ...
... Olsons had babies at the same time at the same hospital. The Jacobsons think that the babies were switched. Electrophoresis can be used to help settle the matter. DNA profiling works in the following manner. 1.Cells are taken from an individual. The cells are broken open and the DNA is extracted. 2. ...
Document
... How is DNA cut at known sites? Restriction endonucleases are enzymes bacteria make to cut foreign DNA (like that from an infecting virus). Each species of bacteria has a “restriction enzyme” that cuts DNA at a unique “palondromic” sequence of 4 to 8 base pairs, called recognition sites. Cutting of ...
... How is DNA cut at known sites? Restriction endonucleases are enzymes bacteria make to cut foreign DNA (like that from an infecting virus). Each species of bacteria has a “restriction enzyme” that cuts DNA at a unique “palondromic” sequence of 4 to 8 base pairs, called recognition sites. Cutting of ...
Modern methods in Molecular Pathology
... simultaneously and visualize co-localization within a single specimen. Using spectrally distinct fluorophore labels for each different hybridization probe, this approach gives you the power to resolve several genetic elements or multiple gene expression patterns in a single specimen, with multicolor ...
... simultaneously and visualize co-localization within a single specimen. Using spectrally distinct fluorophore labels for each different hybridization probe, this approach gives you the power to resolve several genetic elements or multiple gene expression patterns in a single specimen, with multicolor ...
recombinant dna technology
... • FIRST, THE PLASMID IS TREATED WITH THE SAME RESTRICTION ENZYME AS WAS USED TO CREATE THE DNA FRAGMENT • THE RESTRICTION ENZYME WILL CUT THE PLASMID AT THE SAME RECOGNITION SEQUENCES, PRODUCING THE SAME STICKY ENDS CARRIED BY THE FRAGMENTS • MIXING THE FRAGMENTS WITH THE CUT PLASMIDS ALLOWS BASE-PA ...
... • FIRST, THE PLASMID IS TREATED WITH THE SAME RESTRICTION ENZYME AS WAS USED TO CREATE THE DNA FRAGMENT • THE RESTRICTION ENZYME WILL CUT THE PLASMID AT THE SAME RECOGNITION SEQUENCES, PRODUCING THE SAME STICKY ENDS CARRIED BY THE FRAGMENTS • MIXING THE FRAGMENTS WITH THE CUT PLASMIDS ALLOWS BASE-PA ...
71370_Forensic_DNA_Analysis
... • DNA Polymerase = enzyme that builds new DNA strand one base pair at a time ...
... • DNA Polymerase = enzyme that builds new DNA strand one base pair at a time ...
notes
... B: The Structure of DNA 1. Rosiland Frankiln (& Maurice Wilkins) (early 1950’s)– produced photographs (using X-ray diffraction) showing DNA is twisted into a spiral or HELIX with the bases perpendicular to the length of the molecule. Picture also showed that DNA must be composed of more than one str ...
... B: The Structure of DNA 1. Rosiland Frankiln (& Maurice Wilkins) (early 1950’s)– produced photographs (using X-ray diffraction) showing DNA is twisted into a spiral or HELIX with the bases perpendicular to the length of the molecule. Picture also showed that DNA must be composed of more than one str ...
Answers to Problem Set 3A
... Note that the enzymes (numbers) are placed in locations they are likely to be functioning with respect to the way the DNA molecules are presented (ie, the topology of the DNA). This is a simplified view. For example, you could have placed DNA polymerase III (the circled number 6) at either of the t ...
... Note that the enzymes (numbers) are placed in locations they are likely to be functioning with respect to the way the DNA molecules are presented (ie, the topology of the DNA). This is a simplified view. For example, you could have placed DNA polymerase III (the circled number 6) at either of the t ...
lecture5
... Excision Repair, in which the damaged base or bases are removed and then replaced with the correct ones in a localized burst of DNA synthesis. There are three modes of excision repair, each of which employs specialized sets of enzymes. Base Excision Repair (BER) Nucleotide Excision Repair (NER) Mism ...
... Excision Repair, in which the damaged base or bases are removed and then replaced with the correct ones in a localized burst of DNA synthesis. There are three modes of excision repair, each of which employs specialized sets of enzymes. Base Excision Repair (BER) Nucleotide Excision Repair (NER) Mism ...
Nükleik Asitler - mustafaaltinisik.org.uk
... • Nucleotide monomers are joined by 3’-5’ phosphodiester linkages to form nucleic acid (polynucleotide) polymers ...
... • Nucleotide monomers are joined by 3’-5’ phosphodiester linkages to form nucleic acid (polynucleotide) polymers ...
DNA Packaging and Ch..
... Broad course objective: a.) explain the molecular structure of chromosomes as it relates to DNA packaging, chromosome function and gene expression Necessary for future material on: Chromosome Variation, Regulation of Gene Expression DNA Packaging—Why and How • If the DNA in a typical human cell were ...
... Broad course objective: a.) explain the molecular structure of chromosomes as it relates to DNA packaging, chromosome function and gene expression Necessary for future material on: Chromosome Variation, Regulation of Gene Expression DNA Packaging—Why and How • If the DNA in a typical human cell were ...
Chapter 12: Genetic Engineering
... Genetic engineering could not have come about without the development of a ______________________________ to support the process o A way to carefully _________________________ containing the gene away from the genes surrounding it o Find a way to ________________________________ with a piece of DNA ...
... Genetic engineering could not have come about without the development of a ______________________________ to support the process o A way to carefully _________________________ containing the gene away from the genes surrounding it o Find a way to ________________________________ with a piece of DNA ...
Microsoft Word
... amount of nuclear DNA content per cell in plants plays an important role in determining the mode of arrangement of repetitive and single copy DNA sequences. In a study of 16 different plant species, plants with nuclear DNA content less than 4.5 pg are found to have either long period or mixed type o ...
... amount of nuclear DNA content per cell in plants plays an important role in determining the mode of arrangement of repetitive and single copy DNA sequences. In a study of 16 different plant species, plants with nuclear DNA content less than 4.5 pg are found to have either long period or mixed type o ...
Inquiry into Life Twelfth Edition
... monomer nestle into the DNA major grooves in the 2 half-sites – Helices approach each other to hold the two monomers together in the repressor dimer – DNA is similar in shape to B-form DNA – Bending of DNA at the two ends of the DNA fragment as it curves around the repressor ...
... monomer nestle into the DNA major grooves in the 2 half-sites – Helices approach each other to hold the two monomers together in the repressor dimer – DNA is similar in shape to B-form DNA – Bending of DNA at the two ends of the DNA fragment as it curves around the repressor ...
DNA REVIEW Name
... What does tRNA do when its job is finished? Leaves and goes to find another molecule of the same amino acid 23 Uracil is the complement to what other base? Thymine 24 The proteins are made by which cell organelle? The ribosomes This organelle is made of proteins along with which type of RNA? Ribosom ...
... What does tRNA do when its job is finished? Leaves and goes to find another molecule of the same amino acid 23 Uracil is the complement to what other base? Thymine 24 The proteins are made by which cell organelle? The ribosomes This organelle is made of proteins along with which type of RNA? Ribosom ...
BioSc 231 Exam 5 2003
... Multiple Choice. (2 points each) _____ For gene cloning, a geneticist digests DNA with ___ an enzyme that cleaves DNA at sequence-specific sites. A. DNA polymerase B. ligase C. restriction endonuclease D. sticky ends E. cDNA _____ Certain endonucleases cut DNA and leave DNA termini without overhangs ...
... Multiple Choice. (2 points each) _____ For gene cloning, a geneticist digests DNA with ___ an enzyme that cleaves DNA at sequence-specific sites. A. DNA polymerase B. ligase C. restriction endonuclease D. sticky ends E. cDNA _____ Certain endonucleases cut DNA and leave DNA termini without overhangs ...
Introduction and review Lecture 1: Jan. 18, 2006
... showed that the transforming material was sensitive to DNAdegrading enzyme but not to RNA or protein-degrading enzymes. ...
... showed that the transforming material was sensitive to DNAdegrading enzyme but not to RNA or protein-degrading enzymes. ...
Lecture3 (1/22/08) "Nucleic Acids, RNA, and Proteins"
... acids of protein interrogating DNA. Major groove: each base pair forms unique pattern, can be recognized [G-C (AADH); C-G (HDAA)] A-T, T-A (AHA=AHA) and G-C, C-G all look similar (ADA=ADA) ...
... acids of protein interrogating DNA. Major groove: each base pair forms unique pattern, can be recognized [G-C (AADH); C-G (HDAA)] A-T, T-A (AHA=AHA) and G-C, C-G all look similar (ADA=ADA) ...
Slides - nanoHUB
... acids of protein interrogating DNA. Major groove: each base pair forms unique pattern, can be recognized [G-C (AADH); C-G (HDAA)] A-T, T-A (AHA=AHA) and G-C, C-G all look similar (ADA=ADA) ...
... acids of protein interrogating DNA. Major groove: each base pair forms unique pattern, can be recognized [G-C (AADH); C-G (HDAA)] A-T, T-A (AHA=AHA) and G-C, C-G all look similar (ADA=ADA) ...
Learning objectives
... Read pages 336-359 of “Biology” Miller & Levine (Chapter 12) and the photocopied supplements from Biology Campbell & Reece (Chapter 16) to make your Cornell notes and understand the following learning objectives. Remember these are NOT questions but guidelines for your note taking. Reading for compr ...
... Read pages 336-359 of “Biology” Miller & Levine (Chapter 12) and the photocopied supplements from Biology Campbell & Reece (Chapter 16) to make your Cornell notes and understand the following learning objectives. Remember these are NOT questions but guidelines for your note taking. Reading for compr ...
Reg Bio DNA tech 2013 ppt
... purposes (use DNA technology) - identify genes for specific traits - transfer genes from one organism to another ...
... purposes (use DNA technology) - identify genes for specific traits - transfer genes from one organism to another ...
Using DNA to Classify Life
... Until the mid-1970s, taxonomists usually classified life by morphology (shape). For example, a biologist might compare the structure of forelimbs of mammals. In recent years, biologists have also been able to compare the DNA and thus proteins in different organisms. A hypothesis known as the molecul ...
... Until the mid-1970s, taxonomists usually classified life by morphology (shape). For example, a biologist might compare the structure of forelimbs of mammals. In recent years, biologists have also been able to compare the DNA and thus proteins in different organisms. A hypothesis known as the molecul ...
Recombinant DNA Technology
... segment of DNA in a form that can be duplicated in another cell • Requires a – Vector = carrier of DNA segment – Host = cell in which foreign DNA can be duplicated and its gene product may be synthesized ...
... segment of DNA in a form that can be duplicated in another cell • Requires a – Vector = carrier of DNA segment – Host = cell in which foreign DNA can be duplicated and its gene product may be synthesized ...
DNA
Deoxyribonucleic acid (/diˌɒksiˌraɪbɵ.njuːˌkleɪ.ɨk ˈæsɪd/; DNA) is a molecule that carries most of the genetic instructions used in the development, functioning and reproduction of all known living organisms and many viruses. DNA is a nucleic acid; alongside proteins and carbohydrates, nucleic acids compose the three major macromolecules essential for all known forms of life. Most DNA molecules consist of two biopolymer strands coiled around each other to form a double helix. The two DNA strands are known as polynucleotides since they are composed of simpler units called nucleotides. Each nucleotide is composed of a nitrogen-containing nucleobase—either cytosine (C), guanine (G), adenine (A), or thymine (T)—as well as a monosaccharide sugar called deoxyribose and a phosphate group. The nucleotides are joined to one another in a chain by covalent bonds between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone. According to base pairing rules (A with T, and C with G), hydrogen bonds bind the nitrogenous bases of the two separate polynucleotide strands to make double-stranded DNA. The total amount of related DNA base pairs on Earth is estimated at 5.0 x 1037, and weighs 50 billion tonnes. In comparison, the total mass of the biosphere has been estimated to be as much as 4 TtC (trillion tons of carbon).DNA stores biological information. The DNA backbone is resistant to cleavage, and both strands of the double-stranded structure store the same biological information. Biological information is replicated as the two strands are separated. A significant portion of DNA (more than 98% for humans) is non-coding, meaning that these sections do not serve as patterns for protein sequences.The two strands of DNA run in opposite directions to each other and are therefore anti-parallel. Attached to each sugar is one of four types of nucleobases (informally, bases). It is the sequence of these four nucleobases along the backbone that encodes biological information. Under the genetic code, RNA strands are translated to specify the sequence of amino acids within proteins. These RNA strands are initially created using DNA strands as a template in a process called transcription.Within cells, DNA is organized into long structures called chromosomes. During cell division these chromosomes are duplicated in the process of DNA replication, providing each cell its own complete set of chromosomes. Eukaryotic organisms (animals, plants, fungi, and protists) store most of their DNA inside the cell nucleus and some of their DNA in organelles, such as mitochondria or chloroplasts. In contrast, prokaryotes (bacteria and archaea) store their DNA only in the cytoplasm. Within the chromosomes, chromatin proteins such as histones compact and organize DNA. These compact structures guide the interactions between DNA and other proteins, helping control which parts of the DNA are transcribed.First isolated by Friedrich Miescher in 1869 and with its molecular structure first identified by James Watson and Francis Crick in 1953, DNA is used by researchers as a molecular tool to explore physical laws and theories, such as the ergodic theorem and the theory of elasticity. The unique material properties of DNA have made it an attractive molecule for material scientists and engineers interested in micro- and nano-fabrication. Among notable advances in this field are DNA origami and DNA-based hybrid materials.The obsolete synonym ""desoxyribonucleic acid"" may occasionally be encountered, for example, in pre-1953 genetics.