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JCA 2007 (vol 1159, pp 51-57)
JCA 2007 (vol 1159, pp 51-57)

h-Barrel membrane protein folding and structure viewed through the
h-Barrel membrane protein folding and structure viewed through the

video slide - Plattsburgh State Faculty and Research Web Sites
video slide - Plattsburgh State Faculty and Research Web Sites

... • The net flow of K+ ions will continue and the negative charge will increase until the difference in charge between the inside and outside of the cell (which attracts K+ ions back into the cell) balances the effect of the concentration gradient for K+, which is causing K+ ions to flow out. • If it ...
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... Refs. 12–15). It has been suggested that YidC mediates the transfer of TMs from the Sec translocon into the lipid bilayer (1). Indeed, YidC could be co-purified with the Sec translocon, suggesting a physical connection (14). A recent in vitro study using lactose permease (LacY) reveals a novel funct ...
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... membranes is their complexity, as these membranes are typically composed of lipids with many different types of headgroups and acyl chains (Dowhan 1997; Epand 1998). This lipid diversity is likely to be important to ensure the dynamic functioning of membranes under changing environmental conditions, ...
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... the membrane is more permeable to Na+ B. ATP is used to pump Na+ and K+ (further charge separation) ...
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Concentration Dependent Different Action of Tamoxifen on
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... of drug-lipid interactions in biological systems. Therefore, studies are firstly concentrated on drug-model membrane interactions (Toyran and Severcan 2003; Severcan et al. 2005; Gagoś et al. 2004). Tamoxifen (TAM) is a non-steroidal antiestrogen drug, which is widely used to prevent and treat brea ...
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... selective manner, typical reactions of the cortical field potentials as well as of the membrane potential and the postsynaptic potentials of individual neurons are found. These findings are shown in a summarized schematic view in Fig. 2.14. The animal experiments on which Fig. 2.14 is based were car ...
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... with passive electrical structure, four voltage-dependent currents: fast Na+ , IN a ; fast K+ , IKv ; slow non-inactivating K+ , IKm ; and high-voltage activated Ca2+ -dependent current, ICa and one Ca2+ -dependent current, IKCa . All currents were calculated using conventional Hodgkin-Huxleystyle k ...
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... attached to vesicles that contain a neurotransmitter chemical. 5. These proteins change shape, causing the membranes of some "docked" vesicles to fuse with the membrane of the presynaptic cell, thereby opening the vesicles and dumping their neurotransmitter contents into the synaptic cleft, the narr ...
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... tetraethylammonium bromide (TEA) was used, it replaced NaCI on an equimolar basis. All experiments were conducted at room temperature (22-24°C). Cells were visualized by means of a Newvicon-tube TV camera (model 1550; Panasonic) attached to a side port of an ICM-405 inverted microscope (Carl Zeiss, ...
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Membrane potential



Membrane potential (also transmembrane potential or membrane voltage) is the difference in electric potential between the interior and the exterior of a biological cell. With respect to the exterior of the cell, typical values of membrane potential range from –40 mV to –80 mV.All animal cells are surrounded by a membrane composed of a lipid bilayer with proteins embedded in it. The membrane serves as both an insulator and a diffusion barrier to the movement of ions. Ion transporter/pump proteins actively push ions across the membrane and establish concentration gradients across the membrane, and ion channels allow ions to move across the membrane down those concentration gradients. Ion pumps and ion channels are electrically equivalent to a set of batteries and resistors inserted in the membrane, and therefore create a voltage difference between the two sides of the membrane.Virtually all eukaryotic cells (including cells from animals, plants, and fungi) maintain a non-zero transmembrane potential, usually with a negative voltage in the cell interior as compared to the cell exterior ranging from –40 mV to –80 mV. The membrane potential has two basic functions. First, it allows a cell to function as a battery, providing power to operate a variety of ""molecular devices"" embedded in the membrane. Second, in electrically excitable cells such as neurons and muscle cells, it is used for transmitting signals between different parts of a cell. Signals are generated by opening or closing of ion channels at one point in the membrane, producing a local change in the membrane potential. This change in the electric field can be quickly affected by either adjacent or more distant ion channels in the membrane. Those ion channels can then open or close as a result of the potential change, reproducing the signal.In non-excitable cells, and in excitable cells in their baseline states, the membrane potential is held at a relatively stable value, called the resting potential. For neurons, typical values of the resting potential range from –70 to –80 millivolts; that is, the interior of a cell has a negative baseline voltage of a bit less than one-tenth of a volt. The opening and closing of ion channels can induce a departure from the resting potential. This is called a depolarization if the interior voltage becomes less negative (say from –70 mV to –60 mV), or a hyperpolarization if the interior voltage becomes more negative (say from –70 mV to –80 mV). In excitable cells, a sufficiently large depolarization can evoke an action potential, in which the membrane potential changes rapidly and significantly for a short time (on the order of 1 to 100 milliseconds), often reversing its polarity. Action potentials are generated by the activation of certain voltage-gated ion channels.In neurons, the factors that influence the membrane potential are diverse. They include numerous types of ion channels, some of which are chemically gated and some of which are voltage-gated. Because voltage-gated ion channels are controlled by the membrane potential, while the membrane potential itself is influenced by these same ion channels, feedback loops that allow for complex temporal dynamics arise, including oscillations and regenerative events such as action potentials.
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