AMP-activated/SNF1 protein kinases: conserved guardians of
... know almost nothing about the properties of the enzyme from S. pombe, but this structure does define the core interactions between the three subunits, which are likely to be conserved between different eukaryotes. The α-subunits: catalytic subunits. Two α-subunit iso forms (α1/α2) are encoded by di ...
... know almost nothing about the properties of the enzyme from S. pombe, but this structure does define the core interactions between the three subunits, which are likely to be conserved between different eukaryotes. The α-subunits: catalytic subunits. Two α-subunit iso forms (α1/α2) are encoded by di ...
Chapter 4 - Neurasync
... temporal selectivity of CMA relative to other autophagic pathways. Only cytosolic substrates with the loose pentapeptide motif KFERQ or a biochemically related sequence are recognized and degraded through this pathway (1). Furthermore, due to the fact that the pathway depends on discrete protein int ...
... temporal selectivity of CMA relative to other autophagic pathways. Only cytosolic substrates with the loose pentapeptide motif KFERQ or a biochemically related sequence are recognized and degraded through this pathway (1). Furthermore, due to the fact that the pathway depends on discrete protein int ...
Solid-Phase Synthesis, Characterization, and Cellular Activities of
... Current methods of peptide and protein attachment to NDs primarily rely on adsorption over variable time courses.2 These methods introduce variability on the amount of peptide/protein adsorbed to the NDs, as well as pose concerns on the desorption rate prior to the site of intended delivery.11 Coval ...
... Current methods of peptide and protein attachment to NDs primarily rely on adsorption over variable time courses.2 These methods introduce variability on the amount of peptide/protein adsorbed to the NDs, as well as pose concerns on the desorption rate prior to the site of intended delivery.11 Coval ...
pdf file - John Innes Centre
... an excellent model system in which to explore, at the molecular level, the way that effector ligands control the interaction of a PII protein with its target. Deuridylylation of GlnK, as a consequence of ammonium shock, coincides with its membrane sequestration by AmtB (14). The E. coli AmtB-GlnK co ...
... an excellent model system in which to explore, at the molecular level, the way that effector ligands control the interaction of a PII protein with its target. Deuridylylation of GlnK, as a consequence of ammonium shock, coincides with its membrane sequestration by AmtB (14). The E. coli AmtB-GlnK co ...
Ageing and the aggregating proteoglycans of
... appears to be at the N-terminus of the protein, and the smallest of the link proteins appears to be derived from either of the larger by proteolytic cleavage within this region [42]. Irrespective of the heterogeneity, all the link proteins are able to stabilize the proteoglycan aggregate towards dis ...
... appears to be at the N-terminus of the protein, and the smallest of the link proteins appears to be derived from either of the larger by proteolytic cleavage within this region [42]. Irrespective of the heterogeneity, all the link proteins are able to stabilize the proteoglycan aggregate towards dis ...
THE RDOA-DEPENDENT PHOSPHOPROTEOME PROFILE OF Salmonella enterica
... This work also identified changes in the phosphoproteome that were dependent upon the presence or absence of RdoA. Phosphoproteins that showed a significant change in phosphorylation were identified by mass spectroscopy using peptide mass fingerprinting. Proteins identified included protein foldases ...
... This work also identified changes in the phosphoproteome that were dependent upon the presence or absence of RdoA. Phosphoproteins that showed a significant change in phosphorylation were identified by mass spectroscopy using peptide mass fingerprinting. Proteins identified included protein foldases ...
Phosphorylation of Beclin 1 by DAP
... The interaction between BH3-only proteins and the anti-apoptotic Bcl-2 members is mediated by hydrophobic and electrostatic interactions between the amphipathic α-helix of the BH3 domain, and a hydrophobic pocket formed by the BH1-3 domains of the anti-apoptotic Bcl-2 proteins. T119 is a residue uni ...
... The interaction between BH3-only proteins and the anti-apoptotic Bcl-2 members is mediated by hydrophobic and electrostatic interactions between the amphipathic α-helix of the BH3 domain, and a hydrophobic pocket formed by the BH1-3 domains of the anti-apoptotic Bcl-2 proteins. T119 is a residue uni ...
ANALYSIS OF A BACTERIAL SERINE/THREONINE KINASE
... regulatory networks, posttranslational modification of proteins by phosphorylation is a major regulatory mechanism for both eukaryotic and prokaryotic organisms (Johnson and Lewis, 2001; Kennelly, 2003). ...
... regulatory networks, posttranslational modification of proteins by phosphorylation is a major regulatory mechanism for both eukaryotic and prokaryotic organisms (Johnson and Lewis, 2001; Kennelly, 2003). ...
The AMP-activated protein kinase pathway – new
... under cellular conditions. This represents a store of energy that can be used to drive energy-requiring processes. Catabolism (and photosynthesis when available) ‘charges up the battery’ by converting ADP to ATP, whereas most other cellular processes require ATP hydrolysis and tend to discharge the ...
... under cellular conditions. This represents a store of energy that can be used to drive energy-requiring processes. Catabolism (and photosynthesis when available) ‘charges up the battery’ by converting ADP to ATP, whereas most other cellular processes require ATP hydrolysis and tend to discharge the ...
Sequencing Grade Modified Trypsin, Frozen, Product Information
... Description: Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC peptide analysis. In addition, auto-proteolysis can result in th ...
... Description: Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC peptide analysis. In addition, auto-proteolysis can result in th ...
Protein expression by a Beijing strain differs from that of another
... Quantification of ICD in culture supernatants showed no significant difference in the extent of autolysis in the three strains (data not shown). The protein expression profiles were highly similar between the strains, enabling visual comparison. Spot differences consistent between quadruplicate gels ...
... Quantification of ICD in culture supernatants showed no significant difference in the extent of autolysis in the three strains (data not shown). The protein expression profiles were highly similar between the strains, enabling visual comparison. Spot differences consistent between quadruplicate gels ...
Do asparagine-linked carbohydrate chains in glycoproteins have a
... However, there may be a preference of carbohydrate for a localization at external bends of protein structure. Carbohydrate attachment has been observed to occur at positions 21, 22, 34, 62, 76 and 88 in ribonucleases (1, 2, 3, 4, 10). All these positions are localized in or close to fl-turns or to b ...
... However, there may be a preference of carbohydrate for a localization at external bends of protein structure. Carbohydrate attachment has been observed to occur at positions 21, 22, 34, 62, 76 and 88 in ribonucleases (1, 2, 3, 4, 10). All these positions are localized in or close to fl-turns or to b ...
PDF
... not acquired by mammalian oocytes until the completion of a final phase of differentiation immediately before ovulation. During this phase, referred to as the maturation period, many intracellular changes occur, including a reprogramming of protein synthesis and the progression of the cell cycle fro ...
... not acquired by mammalian oocytes until the completion of a final phase of differentiation immediately before ovulation. During this phase, referred to as the maturation period, many intracellular changes occur, including a reprogramming of protein synthesis and the progression of the cell cycle fro ...
The Multiple Personalities of the Regulatory Subunit of Protein
... As the regulatory subunit of a protein kinase that has functions associated with cell cycle progression, CK2β is reminiscent of cyclins that are the regulatory subunits of cyclin-dependent kinases. In a related respect, it is particularly intriguing that CK2β has motifs that have been previously cha ...
... As the regulatory subunit of a protein kinase that has functions associated with cell cycle progression, CK2β is reminiscent of cyclins that are the regulatory subunits of cyclin-dependent kinases. In a related respect, it is particularly intriguing that CK2β has motifs that have been previously cha ...
Degradation of Organelles or Specific Organelle Components via
... Autophagy has been originally considered to be a non-selective process, mediating the bulk degradation of cytosolic components in response to acute stresses. Yet, in recent years, autophagy has also been shown to operate as a selective process, termed selective-autophagy. Selective autophagy operate ...
... Autophagy has been originally considered to be a non-selective process, mediating the bulk degradation of cytosolic components in response to acute stresses. Yet, in recent years, autophagy has also been shown to operate as a selective process, termed selective-autophagy. Selective autophagy operate ...
vts_6580_8973.
... Hematological malignancies are classified according to the cell lineage they originate from. Thus, lymphoblastic or lymphocytic malignancies occur in progenitor or stem cells that will give rise to lymphocytes. In contrast, myeloid or myelogeneous neoplasms derive from progenitor or stem cells which ...
... Hematological malignancies are classified according to the cell lineage they originate from. Thus, lymphoblastic or lymphocytic malignancies occur in progenitor or stem cells that will give rise to lymphocytes. In contrast, myeloid or myelogeneous neoplasms derive from progenitor or stem cells which ...
The dynamics of plant plasma membrane proteins
... localization in root epidermis cells (left). Environmental and intrinsic stimuli, such as incubation in darkness, promote its relocation to the vacuole (right, arrowheads). (B) PIN1, by contrast, accumulates at the basal (rootward) plasma membrane domain of stele cells in root meristems (left), faci ...
... localization in root epidermis cells (left). Environmental and intrinsic stimuli, such as incubation in darkness, promote its relocation to the vacuole (right, arrowheads). (B) PIN1, by contrast, accumulates at the basal (rootward) plasma membrane domain of stele cells in root meristems (left), faci ...
effects of insulin and anchorage on hepatocytic protein metabolism
... furthermore does not seem to be required, since a significant insulin effect can be seen in the presence of the protein synthesis inhibitor, cycloheximide. The issue is, however, complicated by the fact that cycloheximide itself inhibits protein degradation to approximately the same extent as does i ...
... furthermore does not seem to be required, since a significant insulin effect can be seen in the presence of the protein synthesis inhibitor, cycloheximide. The issue is, however, complicated by the fact that cycloheximide itself inhibits protein degradation to approximately the same extent as does i ...
Calcium binding to chromaffin vesicle matrix proteins
... nucleotides, and other low molecular weight components. Memurement of C P Binding. Prior to the analysis of Caz+ binding properties, isolated matrix proteins were dialyzed overnight in a large volume of 20 mM MOPS/KOH or NaOH at pH 7.0 either alone or also containing additional K+, Na+, or Mg”. It s ...
... nucleotides, and other low molecular weight components. Memurement of C P Binding. Prior to the analysis of Caz+ binding properties, isolated matrix proteins were dialyzed overnight in a large volume of 20 mM MOPS/KOH or NaOH at pH 7.0 either alone or also containing additional K+, Na+, or Mg”. It s ...
Evolution of hard proteins in the sauropsid integument in relation to
... Fig. 2 Drawing illustrating the embryonic layers formed on the scale of embryonic crocodilians (A) in comparison with those of bird embryos (B). The arrowed square illustrates the sequence of layers in crocodilian scale (A1) vs. avian beak (C), downfeather (D), claw (E), and scutate scales (F). The ...
... Fig. 2 Drawing illustrating the embryonic layers formed on the scale of embryonic crocodilians (A) in comparison with those of bird embryos (B). The arrowed square illustrates the sequence of layers in crocodilian scale (A1) vs. avian beak (C), downfeather (D), claw (E), and scutate scales (F). The ...
Aminoacyl-tRNA synthetase complexes: beyond translation
... Although aminoacyl-tRNA synthetases (ARSs) are housekeeping enzymes essential for protein synthesis, they can play non-catalytic roles in diverse biological processes. Some ARSs are capable of forming complexes with each other and additional proteins. This characteristic is most pronounced in mammal ...
... Although aminoacyl-tRNA synthetases (ARSs) are housekeeping enzymes essential for protein synthesis, they can play non-catalytic roles in diverse biological processes. Some ARSs are capable of forming complexes with each other and additional proteins. This characteristic is most pronounced in mammal ...
Communication - Department of Molecular Physiology and Biophysics
... binds laminin and dystrophin binds to actin filaments, indicating that one function of the dystrophin-glycoprotein complex is to provide a link between the extracellular matrix and the actin cytoskeleton (5, 6). 59-DAP (now named syntrophin) is a 59kDa cytoplasmic protein triplet in the purified dys ...
... binds laminin and dystrophin binds to actin filaments, indicating that one function of the dystrophin-glycoprotein complex is to provide a link between the extracellular matrix and the actin cytoskeleton (5, 6). 59-DAP (now named syntrophin) is a 59kDa cytoplasmic protein triplet in the purified dys ...
Overview for Electrophoresis and Western Blotting
... We have found that the pH discontinuity of the SDS system plays a much smaller role in stacking proteins than does the ion species discontinuity of the system. In the Laemmli system, the stacking gel pH of 6.8 may serve as an extra boost for the stacking system but is not actually required to stack ...
... We have found that the pH discontinuity of the SDS system plays a much smaller role in stacking proteins than does the ion species discontinuity of the system. In the Laemmli system, the stacking gel pH of 6.8 may serve as an extra boost for the stacking system but is not actually required to stack ...
A CBS domain-containing pyrophosphatase of Moorella
... and its concentration affects the equilibria of these reactions [1]. In addition, PPi regulates many other cellular processes, including calcification, cell proliferation and iron transport [2]. Consequently, disruption of PPi metabolism can lead to a variety of pathological conditions [2]. PPi is m ...
... and its concentration affects the equilibria of these reactions [1]. In addition, PPi regulates many other cellular processes, including calcification, cell proliferation and iron transport [2]. Consequently, disruption of PPi metabolism can lead to a variety of pathological conditions [2]. PPi is m ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.