High Resolution Two-Dimensional Electrophoresis of Proteins*
... them W-labeled E. coli. The labeling period was followed by a chase period of 30 min during which time the nematodes were fed unlabeled E. coli. After this time no labeled E. coli proteins could be detected. Sample ...
... them W-labeled E. coli. The labeling period was followed by a chase period of 30 min during which time the nematodes were fed unlabeled E. coli. After this time no labeled E. coli proteins could be detected. Sample ...
Life 9e - Garvness
... a. Electrons are received from NADH and FADH2. b. Electrons are passed from donor to recipient carrier molecules in a series of oxidation– reduction reactions. c. Usually the terminal electron acceptor is oxygen. d. Most of the enzymes are part of the inner mitochondrial membrane. e. All of the abov ...
... a. Electrons are received from NADH and FADH2. b. Electrons are passed from donor to recipient carrier molecules in a series of oxidation– reduction reactions. c. Usually the terminal electron acceptor is oxygen. d. Most of the enzymes are part of the inner mitochondrial membrane. e. All of the abov ...
Cholesterol Synthesis
... superfamily, with tissue-specific expression and intracellular localization highly regulated. ...
... superfamily, with tissue-specific expression and intracellular localization highly regulated. ...
Details of the scope analysis for each organism
... ATP + H+ + pantetheine 4'-phosphate diphosphate + dephospho-CoA The reaction catalyzed by the nicotinic acid mononucleotide adenylytransferase (EC 2.7.7.18) is proved to be reversible in a biochemical assay in E. coli [53]. However, as the E. coli reconstruction considers this reaction as irrevers ...
... ATP + H+ + pantetheine 4'-phosphate diphosphate + dephospho-CoA The reaction catalyzed by the nicotinic acid mononucleotide adenylytransferase (EC 2.7.7.18) is proved to be reversible in a biochemical assay in E. coli [53]. However, as the E. coli reconstruction considers this reaction as irrevers ...
Glycolytic strategy as a tradeoff between energy yield and protein cost
... enzymes and a pyruvate kinase (pyk) (Materials and Methods, SI Text, and Tables S2 and S3). Z. mobilis, for example, is not genetically capable of the EMP pathway because it has no pfk, whereas Bacillus subtilis is not capable of the ED pathway because it lacks a functional edd enzyme (9). Fig. 2 sh ...
... enzymes and a pyruvate kinase (pyk) (Materials and Methods, SI Text, and Tables S2 and S3). Z. mobilis, for example, is not genetically capable of the EMP pathway because it has no pfk, whereas Bacillus subtilis is not capable of the ED pathway because it lacks a functional edd enzyme (9). Fig. 2 sh ...
Protection by chlorpromazine, albumin and bivalent cations against
... other inhibitors, such as chlorpromazine, albumin and plasma proteins, to interfere with peptide–membrane interactions. Inhibitory analysis is useful for understanding the molecular mechanism of haemolysis because inhibitors can specifically or non-specifically interact with constituents of melittin ...
... other inhibitors, such as chlorpromazine, albumin and plasma proteins, to interfere with peptide–membrane interactions. Inhibitory analysis is useful for understanding the molecular mechanism of haemolysis because inhibitors can specifically or non-specifically interact with constituents of melittin ...
Proton transfer pathways and mechanism in bacterial reaction centers Minireview
... transfer s 100-fold. This suggests that at least two parallel routes are operational in the pathway regions near the proton entry points (see Fig. 1). In addition, there are other possible proton transfer routes in the interior of the protein that may include other protonatable residues (e.g. Glu-H1 ...
... transfer s 100-fold. This suggests that at least two parallel routes are operational in the pathway regions near the proton entry points (see Fig. 1). In addition, there are other possible proton transfer routes in the interior of the protein that may include other protonatable residues (e.g. Glu-H1 ...
A Personal Account of Chaperonin History
... reconstitution of enzymatic activity occurred in the absence of the complete system (GroEL, GroES, and MgATP). Second, there was a fourth component needed that we luckily included, a monovalent cation K⫹ or NH4⫹. Our preparations of GroEL and GroES were stored as ammonium sulfate precipitates, and e ...
... reconstitution of enzymatic activity occurred in the absence of the complete system (GroEL, GroES, and MgATP). Second, there was a fourth component needed that we luckily included, a monovalent cation K⫹ or NH4⫹. Our preparations of GroEL and GroES were stored as ammonium sulfate precipitates, and e ...
Intestinal peptidases form functional complexes with the neutral
... members belong to the gluzincin metalloprotease family, with two consensus zinc-binding sequences, HEXXH and BXLXE (zincbinding residues are indicated in bold, B indicates a bulky sidechain residue and X denotes any residue) [15]. In addition, a third consensus site GXMEN is an exopeptidase substrat ...
... members belong to the gluzincin metalloprotease family, with two consensus zinc-binding sequences, HEXXH and BXLXE (zincbinding residues are indicated in bold, B indicates a bulky sidechain residue and X denotes any residue) [15]. In addition, a third consensus site GXMEN is an exopeptidase substrat ...
COMPLEX FORMATION AND PROTEIN INTERACTION IN THE
... Arabidopsis CesA N-terminal domains. Previously, heterologously-expressed cotton N-terminal domains have been shown to dimerize, in a redox dependent manner, through their zinc binding domains (Kurek et al. 2002). However, assembly of a functional CSC requires interaction between three unique CesA i ...
... Arabidopsis CesA N-terminal domains. Previously, heterologously-expressed cotton N-terminal domains have been shown to dimerize, in a redox dependent manner, through their zinc binding domains (Kurek et al. 2002). However, assembly of a functional CSC requires interaction between three unique CesA i ...
CELLULAR RESPIRATION
... CELLULAR RESPIRATION Energy-Releasing Pathways Anaerobic Definition Energy exchange occurring in the cell cytoplasm that does not use oxygen as the final electron acceptor. Aerobic Definition Energy exchange occurring in the mitochondria using oxygen as the final electron acceptor. ...
... CELLULAR RESPIRATION Energy-Releasing Pathways Anaerobic Definition Energy exchange occurring in the cell cytoplasm that does not use oxygen as the final electron acceptor. Aerobic Definition Energy exchange occurring in the mitochondria using oxygen as the final electron acceptor. ...
Glycolysis
... NADH is oxidized to NAD+. Lactate, in addition to being an end-product of fermentation, serves as a mobile form of nutrient energy, & possibly as a signal molecule in mammalian organisms. Cell membranes contain carrier proteins that facilitate transport of lactate. ...
... NADH is oxidized to NAD+. Lactate, in addition to being an end-product of fermentation, serves as a mobile form of nutrient energy, & possibly as a signal molecule in mammalian organisms. Cell membranes contain carrier proteins that facilitate transport of lactate. ...
Fatty acid
... Overview: Carbon: The Backbone of Life • Although cells are 70–95% water, the rest consists mostly of carbon-based compounds ...
... Overview: Carbon: The Backbone of Life • Although cells are 70–95% water, the rest consists mostly of carbon-based compounds ...
Metabolite transport across the peroxisomal membrane
... for β-oxidation into the peroxisomal matrix comes from studies of the yeast S. cerevisiae. The peroxisomal membrane in S. cerevisiae contains two members of the superfamily of ABC transporters, Pxa1p and Pxa2p [17,18]. Hettema et al. [19] found that the β-oxidation of oleic acid (C18:1 ) is impaired ...
... for β-oxidation into the peroxisomal matrix comes from studies of the yeast S. cerevisiae. The peroxisomal membrane in S. cerevisiae contains two members of the superfamily of ABC transporters, Pxa1p and Pxa2p [17,18]. Hettema et al. [19] found that the β-oxidation of oleic acid (C18:1 ) is impaired ...
Audiometry–Anatomy of the ear – 3064-1/HLSP
... auditory meatus also contains hair follicles. These hairs in conjunction with the wax help to keep foreign bodies and dirt from reaching the inner two thirds of the external auditory meatus. Many people think wax is dirty but as you can see it serves a very important function. As our ears never stop ...
... auditory meatus also contains hair follicles. These hairs in conjunction with the wax help to keep foreign bodies and dirt from reaching the inner two thirds of the external auditory meatus. Many people think wax is dirty but as you can see it serves a very important function. As our ears never stop ...
Systematic Characterisation of Cellular Localisation and
... class I ligand-containing proteins being localised to the cytoplasm, may be found in the size of the studies: The present study includes more than five times as many MHC class I ligand-containing proteins as the earlier study. Another difference between the two studies is that the present study is n ...
... class I ligand-containing proteins being localised to the cytoplasm, may be found in the size of the studies: The present study includes more than five times as many MHC class I ligand-containing proteins as the earlier study. Another difference between the two studies is that the present study is n ...
Gluconeogenesis
... starvation is mainly amino acid catabolism. Some amino acids are catabolized to pyruvate, oxaloacetate, or precursors of these. Muscle proteins may break down to supply amino acids. These are transported to liver where they are deaminated and converted to gluconeogenesis inputs. Glycerol, derived fr ...
... starvation is mainly amino acid catabolism. Some amino acids are catabolized to pyruvate, oxaloacetate, or precursors of these. Muscle proteins may break down to supply amino acids. These are transported to liver where they are deaminated and converted to gluconeogenesis inputs. Glycerol, derived fr ...
Multiple Sequence Motifs in the Rubisco Small
... E1a-tp. TP cleavage sites were predicted by TargetP (Emanuelsson et al., 2000) and indicated by arrowheads. B, Images of RbcStp:GFP and E1a-tp:GFP. Protoplasts from wild-type plants were transformed with RbcS-tp:GFP and E1a-tp:GFP and images were taken at 8 h after transformation. C, Western-blot an ...
... E1a-tp. TP cleavage sites were predicted by TargetP (Emanuelsson et al., 2000) and indicated by arrowheads. B, Images of RbcStp:GFP and E1a-tp:GFP. Protoplasts from wild-type plants were transformed with RbcS-tp:GFP and E1a-tp:GFP and images were taken at 8 h after transformation. C, Western-blot an ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
... directly by a MALDI-TOF mass spectrometer in order to determine their cleavage sites. Since whether a peptide fragment can be detected by the MALDI-TOF mass spectrometer depends on the matrix employed and the molecular mass of that fragment, two different matrices were used. One was a mixture of sin ...
... directly by a MALDI-TOF mass spectrometer in order to determine their cleavage sites. Since whether a peptide fragment can be detected by the MALDI-TOF mass spectrometer depends on the matrix employed and the molecular mass of that fragment, two different matrices were used. One was a mixture of sin ...
Yeast lipid metabolism at a glance
... of organelles (Simons & Sampaio, 2011) whose lipid composition varies. As examples, the plasma membrane is enriched in PS, whereas cardiolipin (CL) and phosphatidylglycerol (PG) are predominantly found in mitochondria (Zinser et al., 1991). Besides PL, sphingolipids (SL) and sterols also contribute ...
... of organelles (Simons & Sampaio, 2011) whose lipid composition varies. As examples, the plasma membrane is enriched in PS, whereas cardiolipin (CL) and phosphatidylglycerol (PG) are predominantly found in mitochondria (Zinser et al., 1991). Besides PL, sphingolipids (SL) and sterols also contribute ...
Functional Domains within Fusion Proteins: Prospectives for
... specific interactions mediated by N-terminal fusion peptides are required to form higher order oligomers necessary for membrane fusion (Kliger et al., 1997). Attempts to define the oligomeric state of the HIV-1 envelope glycoprotein have yielded conflicting results. Several reports indicated that th ...
... specific interactions mediated by N-terminal fusion peptides are required to form higher order oligomers necessary for membrane fusion (Kliger et al., 1997). Attempts to define the oligomeric state of the HIV-1 envelope glycoprotein have yielded conflicting results. Several reports indicated that th ...
Thylakoid
A thylakoid is a membrane-bound compartment inside chloroplasts and cyanobacteria. They are the site of the light-dependent reactions of photosynthesis. Thylakoids consist of a thylakoid membrane surrounding a thylakoid lumen. Chloroplast thylakoids frequently form stacks of disks referred to as grana (singular: granum). Grana are connected by intergranal or stroma thylakoids, which join granum stacks together as a single functional compartment.