novel aspects of carnitine function and metabolism
... remained elusive. We show that lauric acid, a MCFA, also depends on the carnitine shuttle to be fully oxidized in the mitochondria. Furthermore, we observed that when the carnitine shuttle is impaired, this MCFA is able to undergo one or two cycles of peroxisomal β-oxidation. This shows that peroxis ...
... remained elusive. We show that lauric acid, a MCFA, also depends on the carnitine shuttle to be fully oxidized in the mitochondria. Furthermore, we observed that when the carnitine shuttle is impaired, this MCFA is able to undergo one or two cycles of peroxisomal β-oxidation. This shows that peroxis ...
Protein translation in Plasmodium parasites
... tRNAs, ribosomal proteins, and the translation elongation factor Tu (EF-Tu), and also a handful of other poorly characterized proteins unrelated to translation (Figure 1). Most proteins resident in the apicoplast are encoded by the nuclear genome, translated in the cytoplasm, and transported into th ...
... tRNAs, ribosomal proteins, and the translation elongation factor Tu (EF-Tu), and also a handful of other poorly characterized proteins unrelated to translation (Figure 1). Most proteins resident in the apicoplast are encoded by the nuclear genome, translated in the cytoplasm, and transported into th ...
lipids and thyroid hormones - Deep Blue
... proteins bind 96% of hepatocyte T3; the minor fraction of T3 in hepatic nuclei plays a major role in promoting gene expression of lipogenic enzymes. In a rat pituitary tumor line, cytosol proteins compete less and nuclei contain 44% of cell T3.156 Recent evidence shows that another energy-dependent, ...
... proteins bind 96% of hepatocyte T3; the minor fraction of T3 in hepatic nuclei plays a major role in promoting gene expression of lipogenic enzymes. In a rat pituitary tumor line, cytosol proteins compete less and nuclei contain 44% of cell T3.156 Recent evidence shows that another energy-dependent, ...
OXIDATIVE PHOSPHORYLATION AND PHOTOPHOSPHORYLATION
... The mitochondrial respiratory chain consists of a series of sequentially acting electron carriers, most of which are integral proteins with prosthetic groups capable of accepting and donating either one or two electrons. Three types of electron transfers occur in oxidative phosphorylation: (1) direc ...
... The mitochondrial respiratory chain consists of a series of sequentially acting electron carriers, most of which are integral proteins with prosthetic groups capable of accepting and donating either one or two electrons. Three types of electron transfers occur in oxidative phosphorylation: (1) direc ...
Cardiac mitochondrial matrix and respiratory complex - AJP
... catalyzes the phosphorylation of many proteins in cell extracts, consistent with a low absolute specificity. Indeed, as stated by the authors of KESTREL, a significant challenge in this type of study is deciphering the kinase phosphorylations that impact function versus neutral sites. Taking this co ...
... catalyzes the phosphorylation of many proteins in cell extracts, consistent with a low absolute specificity. Indeed, as stated by the authors of KESTREL, a significant challenge in this type of study is deciphering the kinase phosphorylations that impact function versus neutral sites. Taking this co ...
Expression and activity of hexokinase in the early mouse embryo
... The maximal activity and Michaelis constant. KM, of hexokinase have been measured in the peri-implantation mouse embryo using an ultramicrofluorescence technique. In addition, transcript detection of the predominant isoenzyme hexokinase I has been determined in single preimplantation mouse embryos a ...
... The maximal activity and Michaelis constant. KM, of hexokinase have been measured in the peri-implantation mouse embryo using an ultramicrofluorescence technique. In addition, transcript detection of the predominant isoenzyme hexokinase I has been determined in single preimplantation mouse embryos a ...
Oxidation of fatty acids in eukaryotes
... Several suspected fatty acid transport proteins have been identified [5]. However, their specific function(s) in fatty acid uptake and their molecular mechanisms remain to be elucidated. Once long-chain fatty acids have crossed the plasma membrane, they either diffuse or are transported to mitochond ...
... Several suspected fatty acid transport proteins have been identified [5]. However, their specific function(s) in fatty acid uptake and their molecular mechanisms remain to be elucidated. Once long-chain fatty acids have crossed the plasma membrane, they either diffuse or are transported to mitochond ...
Planta
... from the condensation of oxaloacetate and glyoxylate by the enzyme oxalomalate lyase (Sekizawa et al. 1966). In this article, the effects of citramalate and oxalomalate on aconitase expression and activity were investigated. These inhibitors reduced aconitase activity and induced citrate accumulatio ...
... from the condensation of oxaloacetate and glyoxylate by the enzyme oxalomalate lyase (Sekizawa et al. 1966). In this article, the effects of citramalate and oxalomalate on aconitase expression and activity were investigated. These inhibitors reduced aconitase activity and induced citrate accumulatio ...
Mitochondrial trans-2-Enoyl-CoA Reductase of Wax Ester
... gracilis is a facultatively anaerobic organelle that produces ATP under aerobic and anaerobic conditions. In the presence of oxygen, pyruvate from glycolysis enters the mitochondrion and ...
... gracilis is a facultatively anaerobic organelle that produces ATP under aerobic and anaerobic conditions. In the presence of oxygen, pyruvate from glycolysis enters the mitochondrion and ...
lecture 6 ppt
... For glycolysis, coenzyme junction, and the citric acid cycle, give inputs, outputs, ATP made/used, e- carriers loaded, water and carbon dioxide molecules produced ...
... For glycolysis, coenzyme junction, and the citric acid cycle, give inputs, outputs, ATP made/used, e- carriers loaded, water and carbon dioxide molecules produced ...
http://doc.rero.ch
... Figure 1. Model peptide for ET studies. The C-terminal non-natural aromatic amino acid residue contains a photoexcitable 2-(2,2dimethyl-propionyl)-3-(diphenoxyphosphoryloxy)tetrahydrofuran-2ylmethyl group in the 4-position that forms a tetrahydrofuran radical cation 4 upon UV exposure. If X can be o ...
... Figure 1. Model peptide for ET studies. The C-terminal non-natural aromatic amino acid residue contains a photoexcitable 2-(2,2dimethyl-propionyl)-3-(diphenoxyphosphoryloxy)tetrahydrofuran-2ylmethyl group in the 4-position that forms a tetrahydrofuran radical cation 4 upon UV exposure. If X can be o ...
THESIS VITRIFICATION OF IN VITRO- AND IN VIVO
... are reduced. Use of this media should therefore have less chance of sodium and calcium toxicity, and could deter apoptosis. The BLK vitrification method is both easier to use and more consistent. In Experiment 2, we sought to identify the most efficacious cryopreservation method for in vitro-produce ...
... are reduced. Use of this media should therefore have less chance of sodium and calcium toxicity, and could deter apoptosis. The BLK vitrification method is both easier to use and more consistent. In Experiment 2, we sought to identify the most efficacious cryopreservation method for in vitro-produce ...
A new simple fluorimetric method to assay cytosolic ATP content
... seedlings (Petrussa et al. 2001; Chiandussi et al. 2002; Casolo et al. 2003; Petrussa et al. 2004), soybean cell cultures (Casolo et al. 2005), embryogenic cultures of Picea abies (L.) Karst., Abies cephalonica Loud (Petrussa et al. 2008a), Abies alba Mill. (Petrussa et al. 2009), and Arum spadix an ...
... seedlings (Petrussa et al. 2001; Chiandussi et al. 2002; Casolo et al. 2003; Petrussa et al. 2004), soybean cell cultures (Casolo et al. 2005), embryogenic cultures of Picea abies (L.) Karst., Abies cephalonica Loud (Petrussa et al. 2008a), Abies alba Mill. (Petrussa et al. 2009), and Arum spadix an ...
Fungal denitrification and nitric oxide reductase cytochrome P450nor
... P450nor. The fungal system seems to lack Nos and thus the final product is N2O. Both systems were shown to function as the mitochondrial anaerobic respiration [14]. Fusarium oxysporum MT811 also contains dNar that resembles the bacterial counterpart, NarGHI. The fungal system of F. oxysporum MT811 i ...
... P450nor. The fungal system seems to lack Nos and thus the final product is N2O. Both systems were shown to function as the mitochondrial anaerobic respiration [14]. Fusarium oxysporum MT811 also contains dNar that resembles the bacterial counterpart, NarGHI. The fungal system of F. oxysporum MT811 i ...
Beta-ketothiolase deficiency
... severe hypoglycemia, had elevated lactate level, mild hyperammonemia, and ketonuria on the second day of life. No further acidotic episode occurred. The patient was severely retarded with marked restless, choreathetoid movements, marked hypotonia. In these 2 years, several patients with this disorde ...
... severe hypoglycemia, had elevated lactate level, mild hyperammonemia, and ketonuria on the second day of life. No further acidotic episode occurred. The patient was severely retarded with marked restless, choreathetoid movements, marked hypotonia. In these 2 years, several patients with this disorde ...
Sugar Metabolism in Yeasts: an Overview of Aerobic and Anaerobic
... literature one should stress that the Crabtree effect has received special attention from several research teams and, even today, a lack of consensus in terms of its definition is observed. De Deken (1966) described the Crabtree effect as “...the phenotypic expression of a regulatory system involved ...
... literature one should stress that the Crabtree effect has received special attention from several research teams and, even today, a lack of consensus in terms of its definition is observed. De Deken (1966) described the Crabtree effect as “...the phenotypic expression of a regulatory system involved ...
Polynucleotide Phosphorylase and Mitochondrial
... utilizes Pi for cleaving the substrate (termed phosphorolysis) into two compounds, one of which is a phosphorylated metabolite. When AsV substitutes for Pi, arsenolysis is carried out and an arsenylated metabolite is formed. We have recently provided evidence that these phosphorolytic-arsenolytic en ...
... utilizes Pi for cleaving the substrate (termed phosphorolysis) into two compounds, one of which is a phosphorylated metabolite. When AsV substitutes for Pi, arsenolysis is carried out and an arsenylated metabolite is formed. We have recently provided evidence that these phosphorolytic-arsenolytic en ...
1 Causality, Transfer Entropy and Allosteric
... performs its activity. Protein-protein and protein-DNA interactions, drug action and all processes that depend on signal transduction involve allosteric activity for the system to carry out its normal function. Most known cancer causing mutations lead to the disruption of normal allosteric communica ...
... performs its activity. Protein-protein and protein-DNA interactions, drug action and all processes that depend on signal transduction involve allosteric activity for the system to carry out its normal function. Most known cancer causing mutations lead to the disruption of normal allosteric communica ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... a protein of 1,803 aa. The N-terminal 1,239 aa had extensive similarity with PFO. The C-terminal 564 aa had extensive similarity with NADPH-cytochrome P450 reductases (CPR). Since pEgPNO12 and pEgPNO3 are independent cDNAs for the same mRNA, the PFO-CPR fusion was not a cloning artifact. Furthermore ...
... a protein of 1,803 aa. The N-terminal 1,239 aa had extensive similarity with PFO. The C-terminal 564 aa had extensive similarity with NADPH-cytochrome P450 reductases (CPR). Since pEgPNO12 and pEgPNO3 are independent cDNAs for the same mRNA, the PFO-CPR fusion was not a cloning artifact. Furthermore ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... a protein of 1,803 aa. The N-terminal 1,239 aa had extensive similarity with PFO. The C-terminal 564 aa had extensive similarity with NADPH-cytochrome P450 reductases (CPR). Since pEgPNO12 and pEgPNO3 are independent cDNAs for the same mRNA, the PFO-CPR fusion was not a cloning artifact. Furthermore ...
... a protein of 1,803 aa. The N-terminal 1,239 aa had extensive similarity with PFO. The C-terminal 564 aa had extensive similarity with NADPH-cytochrome P450 reductases (CPR). Since pEgPNO12 and pEgPNO3 are independent cDNAs for the same mRNA, the PFO-CPR fusion was not a cloning artifact. Furthermore ...
The malonyl-CoA insensitive fraction of muscle CPT1 is greater in
... investigators have attributed this non-suppressible fraction to CPT2, the inner mitochondrial membrane enzyme that can be expressed if membranes are damaged during mitochondrial preparation (7;15). We considered an alternative view that muscle might contain a malonyl-CoA insensitive CPT1 fraction, a ...
... investigators have attributed this non-suppressible fraction to CPT2, the inner mitochondrial membrane enzyme that can be expressed if membranes are damaged during mitochondrial preparation (7;15). We considered an alternative view that muscle might contain a malonyl-CoA insensitive CPT1 fraction, a ...
Purification and Characterization of a Novel Pumpkin Short
... maize (Olsen and Huang, 1988), and employing both crude tissue extracts and peroxisomes isolated from normal, carbohydrate-starved, and carbohydrate-fed maize root tips (Hooks et al., 1995). A short-chain ACOX was identified and purified to apparent homogeneity from maize plantlets. It was reported ...
... maize (Olsen and Huang, 1988), and employing both crude tissue extracts and peroxisomes isolated from normal, carbohydrate-starved, and carbohydrate-fed maize root tips (Hooks et al., 1995). A short-chain ACOX was identified and purified to apparent homogeneity from maize plantlets. It was reported ...
Biosketch - NC State University
... to examine the impact of local adipocytes on mitochondrial function and insulin action in neighboring myocytes. We also seek to understand how exercise combats age-related metabolic decline. To this end, we are collaborating with Dr. William Kraus to perform metabolic and transcriptional profiling a ...
... to examine the impact of local adipocytes on mitochondrial function and insulin action in neighboring myocytes. We also seek to understand how exercise combats age-related metabolic decline. To this end, we are collaborating with Dr. William Kraus to perform metabolic and transcriptional profiling a ...
Fatty Acid Oxid
... the plasma membrane fatty acid transporter CD36 Carnitine Palmitoyltransferases I & II (required for transfer of fatty acids into mitochondria) Acyl-CoA Dehydrogenases for various chain lengths of fatty acids Hydroxyacyl-CoA Dehydrogenases for medium & short chain length fatty acids Medium ...
... the plasma membrane fatty acid transporter CD36 Carnitine Palmitoyltransferases I & II (required for transfer of fatty acids into mitochondria) Acyl-CoA Dehydrogenases for various chain lengths of fatty acids Hydroxyacyl-CoA Dehydrogenases for medium & short chain length fatty acids Medium ...
Acute nutritional ketosis: implications for exercise performance and metabolism Open Access
... to reinstate ‘Randle cycle’ competition between substrates for oxidative respiration [60,61]. Current literature on dietary fuel selection mechanisms has not considered the role of ketone bodies as a major fuel source in great depth, although the latter are well known to be metabolised by skeletal m ...
... to reinstate ‘Randle cycle’ competition between substrates for oxidative respiration [60,61]. Current literature on dietary fuel selection mechanisms has not considered the role of ketone bodies as a major fuel source in great depth, although the latter are well known to be metabolised by skeletal m ...