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Full-Text PDF
Full-Text PDF

... suspected to be involved in Parkinson’s [12] and Huntington's diseases [13]. Because of these numerous pathways and interconnected cellular functions, many genes are involved, and mutations in these genes may lead to the various diseases mentioned. Almost all these genes are encoded by the nuclear g ...
Characterization of microbial activity in the surface layers of a
Characterization of microbial activity in the surface layers of a

... therefore, was virtually the same in all cases. The relative heterotrophic activity therefore, should b e accurate since the ratio of bottom water to sediment was always approximately 100:1, effectively negating any small differences in the chemistry of the pore waters. The actual uptake rates for g ...
emboj7601705-sup
emboj7601705-sup

... Supplementary Figure 2 Removal of Gsc re-specifies dorsal mesodermal fates in a dose-dependent manner. Embryos were injected four times at 2- to 4-cell stage with increasing amounts of Gsc MO (as indicated on the abscissa). Dorsal marginal zone (DMZ) explants were excised at stage 10 and cultured u ...
The bovine papillomavirus type 4 long control region contains an
The bovine papillomavirus type 4 long control region contains an

Active Site of Ribonuclease A - Department of Biochemistry | UW
Active Site of Ribonuclease A - Department of Biochemistry | UW

... cleavage of dinucleotide substrates and the hydrolysis of nucleoside 2¢,3¢cyclic phosphate substrates (Herries et al. 1962; Witzel 1963; del Rosario and Hammes 1969). This profile is consistent with a mechanism that involves two titratable residues, one of which is protonated and the other unprotona ...
Internal expression of Yarrowia NDH2
Internal expression of Yarrowia NDH2

... 1×10–5 (Barth and Gaillardin, 1996). In our experiment, 5′FOA resistant colonies appeared with approximately 10 times lower frequency. Southern blot analysis of ten colonies demonstrated that, although the URA3 marker had been lost or damaged by spontaneous mutations in all of these strains, the pla ...
File
File

... the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most significant advance in the history of biology, with profound consequences for the future of our, an ...
Analysis of acid-induced asr gene promoter of Enterobacteriaceae
Analysis of acid-induced asr gene promoter of Enterobacteriaceae

The Anaerobic (Class III) Ribonucleotide Reductase from Lactococcus lactis
The Anaerobic (Class III) Ribonucleotide Reductase from Lactococcus lactis

... were designed to generate NdeI and XhoI restriction sites, respectively, at the start and the end of the PCR fragment. The amplified product (630 bp) was purified and cloned into plasmid pGEM-T (Promega), giving plasmid pIG021. After digestion with NdeI and XhoI, the nrdG fragment was ligated into p ...
Classification of pseudo pairs between nucleotide bases and amino
Classification of pseudo pairs between nucleotide bases and amino

... can geometrically make pseudo pairs through N1–H(G) . . . O(Asn/Gln) and O6(G) . . . H–N(Asn/Gln) as proposed by Cheng et al. (26), these pairs were not observed either by them or by us. Both uracil and thymine pair with Asn and Gln in two different geometries at their Watson–Crick edges. In both ps ...
Late events of translation initiation in bacteria: a kinetic analysis
Late events of translation initiation in bacteria: a kinetic analysis

... ¯uorescence changes to particular steps was taken from the poly(U) system (Pape et al., 1998, and references cited therein). The increase in pro¯avin ¯uorescence re¯ects the steps of initial codon-independent binding of the ternary complex to the ribosome as well as the codon recognition (Scheme 1), ...
Review Antibody flexibility observed in antigen binding and its
Review Antibody flexibility observed in antigen binding and its

... ceiling affinity does not seem to be responsible for the limitation of the antibody architecture because the yeast-surface-displayed antibody has been shown to have 2.1 × 1013 M–1 monovalent affinity [6]. Based on these results, the ceiling affinity or avidity in vivo maturation is considered to be ...
e Study of RNA Polymerase Pausing by Optical Traps
e Study of RNA Polymerase Pausing by Optical Traps

... RNAP repeatedly transcribing the pause sequences, thus slowing transcriptional motion and thereby increasing the probability of an RNAP pause at subsequent sites. ...
The Johns Hopkins University - American University of Beirut
The Johns Hopkins University - American University of Beirut

... • Servers have the records you want • You need to understand the data they have, and how it is organized • There are often many ways to get to an answer. • Route to get there is not always obvious, but you need to think of alternatives and traps. • Use some query language – each system has its own. ...
Slide 1
Slide 1

... Substrate-level phosphorylation ...
PDF File
PDF File

... Tetrahymena group I RNA enzyme described herein directly demonstrate the catalytic contributions of binding interactions. With wild-type ribozyme, specific functional groups at a distance from the site of chemical transformation facilitate substrate binding without accelerating reaction of bound sub ...
Slide 1
Slide 1

... + H+ and 1 acetyl CoA for each pyruvate molecule, and TCA cycle produces 3 NADH + H+ and 1 FADH2 for each pyruvate molecule for a total of 9 NADH + H+ (x 2.5) to produce 22.5 ATP, 3 FADH2 (x 1.5) to produce 4.5 ATP, and a net gain of 2 ATP in glycolysis and ~1.5 ATP (GTP) in TCA cycle for a grand to ...
Iron-sulfur Clusters in RNA Polymerase: A New Role for an Ancient
Iron-sulfur Clusters in RNA Polymerase: A New Role for an Ancient

... prosthetic groups that serve essential catalytic, structural, and regulatory functions in a wide variety of proteins in modern organisms. The utilization of Fe-S clusters within cells likely originated very early when the Earth was largely oxygen-free, since Fe-S clusters are typically oxygen-labile ...
Structure of the FHA1 Domain of Yeast Rad53 and Identification of
Structure of the FHA1 Domain of Yeast Rad53 and Identification of

Computational Definition of
Computational Definition of

... splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechanisms in the latter. The strategy we used to overcome the confounding presence of protein coding information was to restrict our search to non-proteincoding exons. In more t ...
Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... on DNA sequence. The latter is in the form of cytosine methylation at CG, CHG and CHH elements (where H = A, T or C) and a variety of histone modifications in nucleosomes. The epialleles arising from cytosine methylation marks on the nuclear genomic loci have better heritability than the epiallelic v ...
Alternatively Spliced Genes
Alternatively Spliced Genes

Score A_c5_17022012
Score A_c5_17022012

File - myrnafoxsciencespot
File - myrnafoxsciencespot

... 3. Energy yields: - occur in two ways: a. substrate level phosphorylation - ATP production results directly from breakdown of substrates (fuel molecules). b. oxidative phosphorylation – ATP production due to transfer of electrons from glucose to O2, via the electron transport chain and chemiosmosis ...
Arctigenin Efficiently Enhanced Sedentary Mice Treadmill
Arctigenin Efficiently Enhanced Sedentary Mice Treadmill

... 29]. Since AMPK activation by actual exercise or pharmacological treatment could lead to up-regulation of PGC-1a gene expression [30,31], we investigated the potential effects of arctigenin on PGC1a mRNA level in both H9C2 and C2C12 cells. Compared with DMSO-treated group, arctigenin incubation coul ...
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