Rolling circle transcription on smallest size double stranded DNA
... other molecules or as programmable assembly chains. I will here describe the principles of using DNA as a structural material; the functions of a few noteworthy DNA nano machines as well as a DNA nanostructure incorporating the concept of a mechanical bond: the DNA rotaxane developed by the Famulok ...
... other molecules or as programmable assembly chains. I will here describe the principles of using DNA as a structural material; the functions of a few noteworthy DNA nano machines as well as a DNA nanostructure incorporating the concept of a mechanical bond: the DNA rotaxane developed by the Famulok ...
Jigsaw handout - the Biology Scholars Program Wiki
... repressor. Lac repressor is a DNA binding protein that binds to a site on the DNA called the operator. The operator partially overlaps with the promoter, which is the site where RNA polymerase binds to initiate transcription. When lac repressor binds to the operator site, RNA polymerase cannot bind ...
... repressor. Lac repressor is a DNA binding protein that binds to a site on the DNA called the operator. The operator partially overlaps with the promoter, which is the site where RNA polymerase binds to initiate transcription. When lac repressor binds to the operator site, RNA polymerase cannot bind ...
NanoString™: User Guide | nCounter® Expression Data Analysis
... hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA targets are pre-mixed with the Reporter CodeSet during manufacturing. Six different transcripts are provided, each at one of the fo ...
... hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA targets are pre-mixed with the Reporter CodeSet during manufacturing. Six different transcripts are provided, each at one of the fo ...
Evolutionary dynamics of RNA-like replicator systems
... that proteins can also catalyze the synthesis of proteins; in fact, proteins are the common catalysts of various chemical ...
... that proteins can also catalyze the synthesis of proteins; in fact, proteins are the common catalysts of various chemical ...
DNA Methylation Analysis
... a convenient methylation kit from Zymo Research converts unmethylated cytosines to uracils, whereas methylated cytosines are protected and remain cytosine (Figure 1). After this conversion step, determining whether the base at a given locus was converted or not converted provides information on its ...
... a convenient methylation kit from Zymo Research converts unmethylated cytosines to uracils, whereas methylated cytosines are protected and remain cytosine (Figure 1). After this conversion step, determining whether the base at a given locus was converted or not converted provides information on its ...
An SMN-Dependent U12 Splicing Event Essential for Motor
... 2009). Consistent with snRNP dysfunction in SMA, widespread splicing changes have been found in tissues of SMA mice (Zhang et al., 2008). However, as this analysis was performed from late disease stages, it is difficult to discriminate direct effects of SMN deficiency from secondary consequences of ...
... 2009). Consistent with snRNP dysfunction in SMA, widespread splicing changes have been found in tissues of SMA mice (Zhang et al., 2008). However, as this analysis was performed from late disease stages, it is difficult to discriminate direct effects of SMN deficiency from secondary consequences of ...
The Structural, Biochemical, and Genetic Characterization of a New
... contain defective chloroplasts, in which the thylakoid membranes are presented as long, parallel structures with little or no overlap. No starch grains have been detected in the mutant chloroplasts. Small vesicles and plastoglobuli can be found within the defective chloroplasts. Genetic studies reve ...
... contain defective chloroplasts, in which the thylakoid membranes are presented as long, parallel structures with little or no overlap. No starch grains have been detected in the mutant chloroplasts. Small vesicles and plastoglobuli can be found within the defective chloroplasts. Genetic studies reve ...
Stress Induction of Mitochondrial Formate Dehydrogenase in Potato
... which was subsequently identified in our laboratory as FDH. In this paper we describe the expression of potato mitochondrial FDH in various tissues and under several stresses. The mRNA levels of the mitochondrial SHMT isoform were studied in parallel because this enzyme was previously described to b ...
... which was subsequently identified in our laboratory as FDH. In this paper we describe the expression of potato mitochondrial FDH in various tissues and under several stresses. The mRNA levels of the mitochondrial SHMT isoform were studied in parallel because this enzyme was previously described to b ...
Acyl Carrier Protein (ACP) lmport into Chloroplasts Does not
... smaller form of pre-ACP import is apo-ACP. Two important points emerged from this mutational analysis. First, the larger import product arises from a modification that depends on the presence of serine 38. Second, the phosphopantetheine prosthetic group, which cannot be added to pre-ACPASer, is not ...
... smaller form of pre-ACP import is apo-ACP. Two important points emerged from this mutational analysis. First, the larger import product arises from a modification that depends on the presence of serine 38. Second, the phosphopantetheine prosthetic group, which cannot be added to pre-ACPASer, is not ...
Lesson 15a Components of DNA #1 PPT
... all cells in all living organisms. DNA controls all the chemical changes which take place in cells. The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA. The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
... all cells in all living organisms. DNA controls all the chemical changes which take place in cells. The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA. The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
Ribosomes of Mouse Liver following
... membrane-associated polysomes would be more severely affected by reactive metabolites than their free counter parts. This possibility was not substantiated by the finding that the sedimentation profiles of both free and membranebound ribosomes contained greatly increased peaks of monomers following ...
... membrane-associated polysomes would be more severely affected by reactive metabolites than their free counter parts. This possibility was not substantiated by the finding that the sedimentation profiles of both free and membranebound ribosomes contained greatly increased peaks of monomers following ...
Novel regulatio pendage transformation
... of the ‘trunk’ genes Ubx and abd-A in a homeotic-like transformation of the anterior thoracic legs into maxillipeds independently in several crustacean lineages. In particular, the absence of UBX and ABD-A from anterior segments of certain derived malacostracan crustaceans correlates with the appear ...
... of the ‘trunk’ genes Ubx and abd-A in a homeotic-like transformation of the anterior thoracic legs into maxillipeds independently in several crustacean lineages. In particular, the absence of UBX and ABD-A from anterior segments of certain derived malacostracan crustaceans correlates with the appear ...
Pseudomon-1 motif
... decision in the present work to group bacteria at the level of order, rather than the more-broad phylum or class, also might help to reduce spurious sequences in clusters. 3. The use of environmental sequences helped to find RNAs that are not well represented in organisms whose genomes have been fu ...
... decision in the present work to group bacteria at the level of order, rather than the more-broad phylum or class, also might help to reduce spurious sequences in clusters. 3. The use of environmental sequences helped to find RNAs that are not well represented in organisms whose genomes have been fu ...
Transvection and pairing of a Drosophila Hox long noncoding RNA
... observed, but almost always on chromosomes where lincX was also expressed. Further, we found that the transvection at lincX was associated with chromosomal pairing at or near the lincX locus. Transvection in the Hox complex is common, associated with lncRNAs, and may be a consequence of a gene regul ...
... observed, but almost always on chromosomes where lincX was also expressed. Further, we found that the transvection at lincX was associated with chromosomal pairing at or near the lincX locus. Transvection in the Hox complex is common, associated with lncRNAs, and may be a consequence of a gene regul ...
... iii) What assumption did you make about the type of binding when you sketched these curves? (2 pts). The hyperbolic curves in the binding curve, and the slope of 1 in the Hill plot indicate noncooperative binding iv) Which kinetic parameter, the on-rate or the off-rate, would you expect to change as ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... including a gap of 63 amino acids and an additional 16 amino acids at the carboxyl terminus, which may play some role in modifying the U-DNA interaction, such that a promoter sequence unique to C. trachomatis is recognized. Monoclonal antibodies specific for E. coli U” were used to probe for homolog ...
... including a gap of 63 amino acids and an additional 16 amino acids at the carboxyl terminus, which may play some role in modifying the U-DNA interaction, such that a promoter sequence unique to C. trachomatis is recognized. Monoclonal antibodies specific for E. coli U” were used to probe for homolog ...
purine
... • Rate of AMP production increases with increasing concentrations of GTP; rate of GMP production increases with increasing concentrations of ATP ...
... • Rate of AMP production increases with increasing concentrations of GTP; rate of GMP production increases with increasing concentrations of ATP ...
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... conserved in almost every KH domain of different proteins (57,58). This further suggested that the RNA-binding property of FMRP is critical for its functions. Also two autosomal homologs of the FMR1 gene, FXR1 and FXR2, have been identified and the overall structures of the corresponding proteins ar ...
... conserved in almost every KH domain of different proteins (57,58). This further suggested that the RNA-binding property of FMRP is critical for its functions. Also two autosomal homologs of the FMR1 gene, FXR1 and FXR2, have been identified and the overall structures of the corresponding proteins ar ...
Epigenetic and genetic factors affect transgene
... controlled by both an epigenetic factor and a genetic factor. The epigenetic factor reflects the methylation phenotype of the sire; those sires with the low somatic phenotype giving rise to offspring with either the low or intermediate phenotype; and those sires with the high somatic phenotype givin ...
... controlled by both an epigenetic factor and a genetic factor. The epigenetic factor reflects the methylation phenotype of the sire; those sires with the low somatic phenotype giving rise to offspring with either the low or intermediate phenotype; and those sires with the high somatic phenotype givin ...
with Non-Insulin-dependent Diabetes Mellitus
... in muscle from patients with NIDDM have been reported. In some ( 17-19) but not all (20-22) studies a reduced GS activity ("total" GS activity) in the presence of a saturating concentration of G6P has been found. Other investigations have shown an impaired GS activity in the presence of physiologica ...
... in muscle from patients with NIDDM have been reported. In some ( 17-19) but not all (20-22) studies a reduced GS activity ("total" GS activity) in the presence of a saturating concentration of G6P has been found. Other investigations have shown an impaired GS activity in the presence of physiologica ...