EPICENTRE Enzyme Catalog
... are resistant to RNase A-type RNases. T7 & SP6 R&DNA™ Polymerases, & DuraScribe™ T7 & SP6 Transcription Kits to synthesize nucleic acids with non-canonical bases or for partial ribosubstitution are covered by U.S. Patents 5,849,546; 6,107,037; or 6,596,494, and other patents issued or pending. These ...
... are resistant to RNase A-type RNases. T7 & SP6 R&DNA™ Polymerases, & DuraScribe™ T7 & SP6 Transcription Kits to synthesize nucleic acids with non-canonical bases or for partial ribosubstitution are covered by U.S. Patents 5,849,546; 6,107,037; or 6,596,494, and other patents issued or pending. These ...
WEREWOLF, a Regulator of Root Hair Pattern
... quantitative (q) RT-PCR showed that only FT expression was reduced in wer mutants under long days, whereas the levels of CO, FD, and SOC1 were similar to the wild type. In short days, there was no difference between the wild type and wer in the transcript level of all four genes. Such a result is co ...
... quantitative (q) RT-PCR showed that only FT expression was reduced in wer mutants under long days, whereas the levels of CO, FD, and SOC1 were similar to the wild type. In short days, there was no difference between the wild type and wer in the transcript level of all four genes. Such a result is co ...
Tetrahydrobiopterin and its functions
... Tetrahydrobiopterin (BH4) is essential for diverse processes and is ubiquitously present in all tissues of higher organisms. It is well established as essential cofactor for various enzyme activities to activate dioxygen, and for less defined functions at the cellular level. The latter function of B ...
... Tetrahydrobiopterin (BH4) is essential for diverse processes and is ubiquitously present in all tissues of higher organisms. It is well established as essential cofactor for various enzyme activities to activate dioxygen, and for less defined functions at the cellular level. The latter function of B ...
Kreb`s Cycle
... – Impt to both catabolism (breakdown) and anabolism (build-up) of cell’s mol’s – Catabolism of carbohydrates, FA’s, aa’s through pyruvate, acetylCoA Kreb’s ATP ...
... – Impt to both catabolism (breakdown) and anabolism (build-up) of cell’s mol’s – Catabolism of carbohydrates, FA’s, aa’s through pyruvate, acetylCoA Kreb’s ATP ...
Epigenetic Regulation ofbdnfGene Transcription in the
... learning induced differential regulation of exon-specific bdnf mRNAs (I, IV, VI, IX) that was associated with changes in bdnf DNA methylation and altered local chromatin structure. Infusions of zebularine (a DNA methyltransferase inhibitor) significantly altered bdnf DNA methylation and triggered ch ...
... learning induced differential regulation of exon-specific bdnf mRNAs (I, IV, VI, IX) that was associated with changes in bdnf DNA methylation and altered local chromatin structure. Infusions of zebularine (a DNA methyltransferase inhibitor) significantly altered bdnf DNA methylation and triggered ch ...
A Molecular Basis for Multiple Herbicide Resistance in Black
... 1. C120V mutant (incapable of being alkylated in this position by NBD-Cl) is dramatically less inhibited by NBD-Cl than AmGSTF1. 2. C120V mutant has a 15 % higher specific activity toward CDNB than AmGSTF1 (see DMSO control). ...
... 1. C120V mutant (incapable of being alkylated in this position by NBD-Cl) is dramatically less inhibited by NBD-Cl than AmGSTF1. 2. C120V mutant has a 15 % higher specific activity toward CDNB than AmGSTF1 (see DMSO control). ...
Molecular Design of Expression Systems
... have been cloned and utilized to regulate transcription of cloned genes!,25 In each of these systems, the transcription activity of the promoter-operator depends upon interaction with a corresponding specific repressor protein. This interaction may be influenced by adjusting the temperature (ts regu ...
... have been cloned and utilized to regulate transcription of cloned genes!,25 In each of these systems, the transcription activity of the promoter-operator depends upon interaction with a corresponding specific repressor protein. This interaction may be influenced by adjusting the temperature (ts regu ...
Development of the genetic code: Insights from a fungal
... plays a major role in the evolution of genetic code alterations via its biased effects on codon usage [44,46] (Fig. 3A). The theory posits that codons can disappear from genomes due to strong G + C or A + T replication pressure, and is supported by the unassignment of the AGA, AUA codons in Microcco ...
... plays a major role in the evolution of genetic code alterations via its biased effects on codon usage [44,46] (Fig. 3A). The theory posits that codons can disappear from genomes due to strong G + C or A + T replication pressure, and is supported by the unassignment of the AGA, AUA codons in Microcco ...
Genes for two multicopper proteins required for Fe(III) oxide
... multicopper protein OmpB for Fe(III) oxide reduction. A homologue of OmpB, designated OmpC, which is 36 % similar to OmpB, has been discovered in the G. sulfurreducens genome. Deletion of ompC inhibited reduction of insoluble, but not soluble Fe(III). Analysis of multiple Geobacter and Pelobacter ge ...
... multicopper protein OmpB for Fe(III) oxide reduction. A homologue of OmpB, designated OmpC, which is 36 % similar to OmpB, has been discovered in the G. sulfurreducens genome. Deletion of ompC inhibited reduction of insoluble, but not soluble Fe(III). Analysis of multiple Geobacter and Pelobacter ge ...
FS406-22
... and 95°C (Fig. 1). Agitation of the culture was nec(12). Cluster 2 includes molybdenum dinitrogenase reductases from methanogens, as well as essary for growth on N2, and clusters of two or more alternative vanadium and iron-only dinitrogenase reductases (VnfH, AnfH) from Methanosarcinales cocci were ...
... and 95°C (Fig. 1). Agitation of the culture was nec(12). Cluster 2 includes molybdenum dinitrogenase reductases from methanogens, as well as essary for growth on N2, and clusters of two or more alternative vanadium and iron-only dinitrogenase reductases (VnfH, AnfH) from Methanosarcinales cocci were ...
The biased nucleotide composition of the HIV genome: a constant
... composition has implications for the amino acids that are encoded by the open reading frames (ORFs), both in the requirement of specific tRNA species and in the preference for amino acids encoded by e.g. A-rich codons. Nucleotide composition does obviously affect the secondary and tertiary structure ...
... composition has implications for the amino acids that are encoded by the open reading frames (ORFs), both in the requirement of specific tRNA species and in the preference for amino acids encoded by e.g. A-rich codons. Nucleotide composition does obviously affect the secondary and tertiary structure ...
Biochemistry Lecture 16
... • 2 C’s leave as CO2 (not same 2 C’s that entered) • 4 redox rxn’s – 3 NAD+ 3 NADH; 1 FAD FADH2 • Where will these go? ...
... • 2 C’s leave as CO2 (not same 2 C’s that entered) • 4 redox rxn’s – 3 NAD+ 3 NADH; 1 FAD FADH2 • Where will these go? ...
1 - u.arizona.edu
... - PDH allosterically inhibited by acetyl CoA (E2 component) and NADH (E3 component) - NADH provides electrons for respiratory (electron transport) chain (oxidative phosphorylation) - enough ATP NADH accumulates in mitochondrial matrix shuts off E3 enzyme to prevent mitochondria from unnecessaril ...
... - PDH allosterically inhibited by acetyl CoA (E2 component) and NADH (E3 component) - NADH provides electrons for respiratory (electron transport) chain (oxidative phosphorylation) - enough ATP NADH accumulates in mitochondrial matrix shuts off E3 enzyme to prevent mitochondria from unnecessaril ...
- Philsci-Archive
... relationship between DNA sequence and the proteins for which it “codes” is “specific”. My concern is with what this claim means—with the empirical features that biologists believe this relationship to possess which leads them to think of it as specific -- and only secondarily with the complicated an ...
... relationship between DNA sequence and the proteins for which it “codes” is “specific”. My concern is with what this claim means—with the empirical features that biologists believe this relationship to possess which leads them to think of it as specific -- and only secondarily with the complicated an ...
... The principal reason is that the buried Thr residue does not have an H-bond donor/acceptor since it is in the hydrophobic core. Since this Thr would form an H-bond to water in the unfolded state, it will cost about 20 kJ/mole of instability since a hydrogen bond to water is broken during folding, bu ...
Inhibition of the Particle-associated RNA
... various compounds were tested in parallel in the same experiment using a single virus preparation. Unless otherwise stated, inhibitory compounds at a range of different concentrations from o-I to o.ooi mM were added last to the RNA polymerase reaction mixture at 4 °C, immediately before warming to 3 ...
... various compounds were tested in parallel in the same experiment using a single virus preparation. Unless otherwise stated, inhibitory compounds at a range of different concentrations from o-I to o.ooi mM were added last to the RNA polymerase reaction mixture at 4 °C, immediately before warming to 3 ...
network - bioinf leipzig
... Regulatory interactions can also be inferred directly from data = reverse engineering of biological pathways/networks from data. In the example above time-series expression data61is used to infer a directed and signed graph based on delayed correlations. ...
... Regulatory interactions can also be inferred directly from data = reverse engineering of biological pathways/networks from data. In the example above time-series expression data61is used to infer a directed and signed graph based on delayed correlations. ...
P-Element Transformation with period Locus DNA Restores
... the array of behavioral phenotypes expressed by our transformants is not due merely to the fact that the various transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We ...
... the array of behavioral phenotypes expressed by our transformants is not due merely to the fact that the various transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We ...
Vmax Regulation through Domain and Subunit Changes. The Active
... volumes was used to improve the MAD phases. These modified phases resulted in a FOM of 0.58 to a resolution of 2.24 Å. Automated chain tracing was carried out in RESOLVE, providing an initial model for 78% of the overall structure (19). Only a few errors in the automatic tracing needed correction us ...
... volumes was used to improve the MAD phases. These modified phases resulted in a FOM of 0.58 to a resolution of 2.24 Å. Automated chain tracing was carried out in RESOLVE, providing an initial model for 78% of the overall structure (19). Only a few errors in the automatic tracing needed correction us ...
Gibberellin Regulates Mitochondrial Pyruvate Dehydrogenase
... treated with 5 µM GA3 for 24 h, and enriched mitochondrial fractions were extracted from shoot tissues collected from 2week-old controls and transgenic plants, treated or not with GA3. In the case of the wild type, plants treated with GA3 had reduced PDH activity when normalized for total mitochondr ...
... treated with 5 µM GA3 for 24 h, and enriched mitochondrial fractions were extracted from shoot tissues collected from 2week-old controls and transgenic plants, treated or not with GA3. In the case of the wild type, plants treated with GA3 had reduced PDH activity when normalized for total mitochondr ...
Novel mutations in the folliculin gene associated with spontaneous pneumothorax ´tya
... The nonsense mutation in exon 7 (c.779G.A, p.W260X) may lead to NMD [21]. In order to investigate the effect of the nonsense mutation on RNA expression, the amounts of FLCN transcript in patient III.5 were compared with an unaffected family member (III.1) of comparable age. Indeed, this analysis rev ...
... The nonsense mutation in exon 7 (c.779G.A, p.W260X) may lead to NMD [21]. In order to investigate the effect of the nonsense mutation on RNA expression, the amounts of FLCN transcript in patient III.5 were compared with an unaffected family member (III.1) of comparable age. Indeed, this analysis rev ...