Simplified global gene expression profiling
... Chef-Ready Kit (Cat. No. A31446) for UHRR and HBRR samples. The kit enables users to generate 8 equalized Ion AmpliSeq™ libraries utilizing the robust liquid handling capabilities of the Ion Chef System. The Ion S5 XL System combined with automated library and template preparation on the Ion Chef Sy ...
... Chef-Ready Kit (Cat. No. A31446) for UHRR and HBRR samples. The kit enables users to generate 8 equalized Ion AmpliSeq™ libraries utilizing the robust liquid handling capabilities of the Ion Chef System. The Ion S5 XL System combined with automated library and template preparation on the Ion Chef Sy ...
FOR IBC USE ONLY - University Research Compliance
... If adding personnel, describe in narrative form their qualifications by documenting the relevant experience and training each individual has. Include the number of years of experience and the capacity in which this experience was gained. The PI should include a statement that he/she will bear direct ...
... If adding personnel, describe in narrative form their qualifications by documenting the relevant experience and training each individual has. Include the number of years of experience and the capacity in which this experience was gained. The PI should include a statement that he/she will bear direct ...
Pharmacophore screening of the Protein Data Bank for specific
... required residue types, but also the relative geometry. The number of unique sites selected using various radius (Qm/n) and direction (Qv) b-factors is shown in Fig. 3 (results from the individual pharmacophore queries were merged and redundant hits were clustered). Ultimately, the objective is not ...
... required residue types, but also the relative geometry. The number of unique sites selected using various radius (Qm/n) and direction (Qv) b-factors is shown in Fig. 3 (results from the individual pharmacophore queries were merged and redundant hits were clustered). Ultimately, the objective is not ...
Exam 4 key fall 2010
... Electrons from NADH and FADH2 are transferred to the electron transport chain and move through those molecules ultimately reducing oxygen to water. During this process, protons are transferred out of the mitochondrial matrix into the intermembrane space creating a proton gradient. This gradient is u ...
... Electrons from NADH and FADH2 are transferred to the electron transport chain and move through those molecules ultimately reducing oxygen to water. During this process, protons are transferred out of the mitochondrial matrix into the intermembrane space creating a proton gradient. This gradient is u ...
The relative rates of synthesis of DNA, sRNA and rRNA in the
... F. Determination of relative rates of synthesis Determination of absolute rates of synthesis using incorporation of labelled precursor demands a knowledge of the specific activity of the immediate precursor pool. We have not been able to estimate this in parts of embryos because it would be hard to ...
... F. Determination of relative rates of synthesis Determination of absolute rates of synthesis using incorporation of labelled precursor demands a knowledge of the specific activity of the immediate precursor pool. We have not been able to estimate this in parts of embryos because it would be hard to ...
Structure of mitochondrial ADP/ATP carrier in complex with
... membranes, reveals a deep depression. At its bottom, a hexapeptide carrying the signature of nucleotide carriers (RRRMMM) is located. Our structure, together with earlier biochemical results, suggests that transport substrates bind to the bottom of the cavity and that translocation results from a tr ...
... membranes, reveals a deep depression. At its bottom, a hexapeptide carrying the signature of nucleotide carriers (RRRMMM) is located. Our structure, together with earlier biochemical results, suggests that transport substrates bind to the bottom of the cavity and that translocation results from a tr ...
Ch18.doc
... dehydrogenase (1 NADH) and one turn of the CAC: yielding 3NADH, 1FADH2 and 1 GTP. Converting NADH and FADH2 to ATPs we use 1 NADH = 2.5 ATP and 1 FADH2 = 1.5 ATP. So for one alanine: (3+1)(2.5 ATP) + 1.5 ATP + 1 ATP = 12.5 ATP. But now it will cost some ATP to get rid of the amino group: so it would ...
... dehydrogenase (1 NADH) and one turn of the CAC: yielding 3NADH, 1FADH2 and 1 GTP. Converting NADH and FADH2 to ATPs we use 1 NADH = 2.5 ATP and 1 FADH2 = 1.5 ATP. So for one alanine: (3+1)(2.5 ATP) + 1.5 ATP + 1 ATP = 12.5 ATP. But now it will cost some ATP to get rid of the amino group: so it would ...
Structure of ATP-Bound Human ATP:Cobalamin
... Loss of AdoCbl results in methylmalonic aciduria (MMA), a buildup of methylmalonic acid in body fluids, including blood and urine (12, 16). Mutations in three different genetic loci, cblA, cblB, and mut, specifically result in AdoCbl deficiency. Methylmalonyl CoA-mutase is encoded at the mut locus ( ...
... Loss of AdoCbl results in methylmalonic aciduria (MMA), a buildup of methylmalonic acid in body fluids, including blood and urine (12, 16). Mutations in three different genetic loci, cblA, cblB, and mut, specifically result in AdoCbl deficiency. Methylmalonyl CoA-mutase is encoded at the mut locus ( ...
Gene Finding and Sequence Annotation - Lectures For UG-5
... Sequence homology is a powerful method to detect genes in a genome. However, it assumes that an mRNA sequence is present, which could have been obtained in other (transcriptomics) experiments. An mRNA is an expressed gene. Thus, if we are able to align the mRNA to the genome, then we know the locati ...
... Sequence homology is a powerful method to detect genes in a genome. However, it assumes that an mRNA sequence is present, which could have been obtained in other (transcriptomics) experiments. An mRNA is an expressed gene. Thus, if we are able to align the mRNA to the genome, then we know the locati ...
Respiration: Occurs in two places in the cell Cytoplasm and
... The RXNs of Respiration: Electron Transport Chain Chemiosmosis and the proton (H+) motive force Linking e- transport and H+ shuttling to ATP synthesis NADH + H+ ...
... The RXNs of Respiration: Electron Transport Chain Chemiosmosis and the proton (H+) motive force Linking e- transport and H+ shuttling to ATP synthesis NADH + H+ ...
Molecular Mechanisms of Plant and Microbe Coexistence
... reporter bacteria responding specifically to individual amino acids. The induction of a lysine-responsive P. putida reporter was demonstrated in rhizosphere of corn, but not in the bulk soil (Espinosa-Urgel and Ramos 2001) and a tryptophan-reporter strain showed significant induction in older root s ...
... reporter bacteria responding specifically to individual amino acids. The induction of a lysine-responsive P. putida reporter was demonstrated in rhizosphere of corn, but not in the bulk soil (Espinosa-Urgel and Ramos 2001) and a tryptophan-reporter strain showed significant induction in older root s ...
PURINE Lacture
... sufhydryl groups at the catalytic site of Ribonucleotide Reductase. 2. Active site of RR contains thioredoxin, a 12 kD proteinwith two exposed cysteines, which become oxidized. 3. This ultimately allows for the reduction of ribose. REGULATION 1. Based on the response to cellular need for dATPs. ...
... sufhydryl groups at the catalytic site of Ribonucleotide Reductase. 2. Active site of RR contains thioredoxin, a 12 kD proteinwith two exposed cysteines, which become oxidized. 3. This ultimately allows for the reduction of ribose. REGULATION 1. Based on the response to cellular need for dATPs. ...
Testis-specific TAF homologs collaborate to control a
... raising the possibility that cell-type- or stage-specific forms of what was previously thought of as the general transcription machinery may play an important role in selective activation of certain PolII promoters (Verrijzer, 2001). To date, however, only a few tissue-specific TAFIIs have been inve ...
... raising the possibility that cell-type- or stage-specific forms of what was previously thought of as the general transcription machinery may play an important role in selective activation of certain PolII promoters (Verrijzer, 2001). To date, however, only a few tissue-specific TAFIIs have been inve ...
PPT
... Genomics & Systems Biology • How do patterns of gene expression determine phenotype? • Which genes and proteins are required for differentiation during during development? • How do proteins interact in biological networks? • Which genes and pathways have been most highly conserved during evolution? ...
... Genomics & Systems Biology • How do patterns of gene expression determine phenotype? • Which genes and proteins are required for differentiation during during development? • How do proteins interact in biological networks? • Which genes and pathways have been most highly conserved during evolution? ...
PureLink® RNA Mini Kit - Thermo Fisher Scientific
... RNA yield is dependent on sample type, size, and quality. Depending on your expected RNA yield, and your sample source and starting amount, use between 30 μL–3 × 100 µL RNase Free Water (or Tris-Buffer) for each elution. Example yields for various sample types and amounts are provided on page 59. Ge ...
... RNA yield is dependent on sample type, size, and quality. Depending on your expected RNA yield, and your sample source and starting amount, use between 30 μL–3 × 100 µL RNase Free Water (or Tris-Buffer) for each elution. Example yields for various sample types and amounts are provided on page 59. Ge ...
msb145697-sup-0001-Supp_Info
... fraction for various E. coli strains follows similar linear relation with growth rate when growth is limited by nutrients (Scott et al, 2010). The red dots were estimated from R/P measurements (Supplemental Materials and Methods) of NCM3722 growing on various carbon and nitrogen sources, with the fo ...
... fraction for various E. coli strains follows similar linear relation with growth rate when growth is limited by nutrients (Scott et al, 2010). The red dots were estimated from R/P measurements (Supplemental Materials and Methods) of NCM3722 growing on various carbon and nitrogen sources, with the fo ...
The complete nucleotide sequence of the RNA coding for the
... FIGURE 1 Organisation of the FMDV genome. The positions of the gene products is based on previously published work (1). The naming of the polypeptides has been done using both existing names and the recommendations agreed at the 3rd European Study Group on moleculear biology of picornaviruses, Urbin ...
... FIGURE 1 Organisation of the FMDV genome. The positions of the gene products is based on previously published work (1). The naming of the polypeptides has been done using both existing names and the recommendations agreed at the 3rd European Study Group on moleculear biology of picornaviruses, Urbin ...
Get PDF - Wiley Online Library
... those of the original eIF4GI were detected in the immunoprecipitates. [Figure 2A, lower panel; compare lanes 2 and 3; the expression of HA-extended eIF4GI in the whole cell is one-fifth of that of the original eIF4GI, for unknown reasons. In addition, the extended eIF4GI could not be extracted with ...
... those of the original eIF4GI were detected in the immunoprecipitates. [Figure 2A, lower panel; compare lanes 2 and 3; the expression of HA-extended eIF4GI in the whole cell is one-fifth of that of the original eIF4GI, for unknown reasons. In addition, the extended eIF4GI could not be extracted with ...
mbe.oxfordjournals.org - Oxford Academic
... biogenesis, nucleolar assembly, and regulation of vital cellular processes. However, this nucleolar inventory has not unveiled a specific consensus motif necessary for nucleolar binding. The ribosomal protein family characterized by their basic nature should exhibit distinct binding sequences that e ...
... biogenesis, nucleolar assembly, and regulation of vital cellular processes. However, this nucleolar inventory has not unveiled a specific consensus motif necessary for nucleolar binding. The ribosomal protein family characterized by their basic nature should exhibit distinct binding sequences that e ...
The microRNAs of Caenorhabditis elegans
... Components of regulatory networks: target recognition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Functions of miRNAs III: other miRNAs of C. elegans . . . . . . . . . . . . . ...
... Components of regulatory networks: target recognition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Functions of miRNAs III: other miRNAs of C. elegans . . . . . . . . . . . . . ...
Gene Section NQO1 (NAD(P)H dehydrogenase, quinone 1) Atlas of Genetics and Cytogenetics
... as an activating enzyme. Because of the high levels of NQO1 in certain tumors, this has led to an interest in designing compounds which can be efficiently bioactivated by NQO1 as antitumor agents. ...
... as an activating enzyme. Because of the high levels of NQO1 in certain tumors, this has led to an interest in designing compounds which can be efficiently bioactivated by NQO1 as antitumor agents. ...