TNFα signals through specialized factories where responsive coding
... the large number of p65 foci shown in the supplemental figure. Exactly where do these genes colocalize? Are these colocalization sites actual tx factories, are they a subset of p65 foci which also colocalize with tx factories, are they in fact at other types of well defined nuclear bodies such as sp ...
... the large number of p65 foci shown in the supplemental figure. Exactly where do these genes colocalize? Are these colocalization sites actual tx factories, are they a subset of p65 foci which also colocalize with tx factories, are they in fact at other types of well defined nuclear bodies such as sp ...
Review Molecular Biology in Arteriosclerosis Research
... The topics discussed In this article illustrate how molecular biology will have a dramatic Impact on arteriosclerosis research. DNA clones for a small number of relevant proteins have been isolated, and studies are underway in numerous laboratories to extend these initial studies. The techniques of ...
... The topics discussed In this article illustrate how molecular biology will have a dramatic Impact on arteriosclerosis research. DNA clones for a small number of relevant proteins have been isolated, and studies are underway in numerous laboratories to extend these initial studies. The techniques of ...
miRNA pptx - NUS School of Computing
... MicroRNA (miRNA) is a class of RNA which is believed to play important roles in gene regulation. It’s a short (21- to 23-nt) RNAs that bind to the 3′ untranslated regions (3′ UTRs) of target genes. ...
... MicroRNA (miRNA) is a class of RNA which is believed to play important roles in gene regulation. It’s a short (21- to 23-nt) RNAs that bind to the 3′ untranslated regions (3′ UTRs) of target genes. ...
DNA MUTATION, REPAIR, AND TRANSPOSITION
... Therefore, DNA molecule I is the least sensitive, while molecule III is the most sensitive. 24. Frameshift mutations are caused by insertions or deletions of bases (that are not multiples of 3). These will shift the reading frame for all codons downstream from the mutation. Single base-substitutions ...
... Therefore, DNA molecule I is the least sensitive, while molecule III is the most sensitive. 24. Frameshift mutations are caused by insertions or deletions of bases (that are not multiples of 3). These will shift the reading frame for all codons downstream from the mutation. Single base-substitutions ...
sequence
... Each of the above can have '[start : end]' or '[start : end : r]' appended to them. The 'file' and 'dbname' forms of USA can have 'format::' in front of them (although a database knows which format it is and so this is redundant and error-prone) ...
... Each of the above can have '[start : end]' or '[start : end : r]' appended to them. The 'file' and 'dbname' forms of USA can have 'format::' in front of them (although a database knows which format it is and so this is redundant and error-prone) ...
167 renal and small intestinal sodium
... around 40–70 mmol l21. At neutral pH, interaction with sodium is sigmoidal, with a Hill coefficient of approximately 2, suggesting a stoichiometry of 2Na+/HPO422 (for a review, see Murer et al. 1991). With respect to the interactions of Pi and Na+, apical Na+/Pi symport in small intestine (duodenum) ...
... around 40–70 mmol l21. At neutral pH, interaction with sodium is sigmoidal, with a Hill coefficient of approximately 2, suggesting a stoichiometry of 2Na+/HPO422 (for a review, see Murer et al. 1991). With respect to the interactions of Pi and Na+, apical Na+/Pi symport in small intestine (duodenum) ...
Slide 1
... predictions fails to improve for “stringent” thresholds • For most predictive models this ratio would increase ...
... predictions fails to improve for “stringent” thresholds • For most predictive models this ratio would increase ...
Associated with Interstitial Lung Disease Asparaginyl
... syndrome of inflammatory myopathy with interstitial lung disease (ILD) and arthritis. Serum KS, from a patient with ILD and inflammatory arthritis without evidence of myositis, immunoprecipitated a tRNA that was distinct from that precipitated by any described anti-synthetase or other reported tRNA- ...
... syndrome of inflammatory myopathy with interstitial lung disease (ILD) and arthritis. Serum KS, from a patient with ILD and inflammatory arthritis without evidence of myositis, immunoprecipitated a tRNA that was distinct from that precipitated by any described anti-synthetase or other reported tRNA- ...
Effects of rare codon clusters on high-level expression
... The human R N A polymerase II associating protein KAP74 is another example where arginine (and other rare) codons affect expression [11]. RAP74 was expressed using the E. coli B strain BL21(DE3) T7 polymerase system. The ml:I.NA for this protein contains 10 A G G / A G A codons out of 512, or -2% of ...
... The human R N A polymerase II associating protein KAP74 is another example where arginine (and other rare) codons affect expression [11]. RAP74 was expressed using the E. coli B strain BL21(DE3) T7 polymerase system. The ml:I.NA for this protein contains 10 A G G / A G A codons out of 512, or -2% of ...
The ECF sigma factors of Streptomyces coelicolor A3(2)
... years later using a reverse genetics approach revealed that it belonged, together with E. coli E, to a new subfamily of the 70 family (Lonetto et al., 1994). Members of this new subfamily were sufficiently different from the previously known factors that in many cases they were not identified as ...
... years later using a reverse genetics approach revealed that it belonged, together with E. coli E, to a new subfamily of the 70 family (Lonetto et al., 1994). Members of this new subfamily were sufficiently different from the previously known factors that in many cases they were not identified as ...
Crystal structure of yeast hexokinase Pl in complex
... named DG stat, the discrepancy between amino acid frequencies in a given position and the amino acid frequencies observed in nature, which shows how strongly this position is conserved in the protein family.27 A second parameter, termed DDG stat, quantifies how much the amino acid distribution of a ...
... named DG stat, the discrepancy between amino acid frequencies in a given position and the amino acid frequencies observed in nature, which shows how strongly this position is conserved in the protein family.27 A second parameter, termed DDG stat, quantifies how much the amino acid distribution of a ...
The RAGNYA fold: a novel fold with multiple topological variants
... in order to attain stability. In particular, such a pathway has been invoked to explain origins of some b-barrel folds, like the 6-stranded double c-beta barrel (DPBB) and the EI-barrel folds, which are found in several ancient domains with major roles in nucleic acid binding and metabolism (9,10,17 ...
... in order to attain stability. In particular, such a pathway has been invoked to explain origins of some b-barrel folds, like the 6-stranded double c-beta barrel (DPBB) and the EI-barrel folds, which are found in several ancient domains with major roles in nucleic acid binding and metabolism (9,10,17 ...
Evolution of Protein Structure - Theoretical and Computational
... and deletions and has been shown to produce accurate structure-based phylogenetic trees. The STAMP structural alignment algorithm, kindly provided by our colleagues Russell and Barton, is included in our alpha release 2 . We plan to offer biomedical researchers a tool to examine the changes in prote ...
... and deletions and has been shown to produce accurate structure-based phylogenetic trees. The STAMP structural alignment algorithm, kindly provided by our colleagues Russell and Barton, is included in our alpha release 2 . We plan to offer biomedical researchers a tool to examine the changes in prote ...
2 Identifying Conserved Elements in the Toxin Sensor and
... Institute of Health (NIH). GenBank is the annotated collection of publicly available gene sequences. By the end of 2013 there was 169,331,407 sequences stored in the GenBank, which represents a 17-fold increase in data since the sequencing of the human genome in 2000 and an about 280,000-fold increa ...
... Institute of Health (NIH). GenBank is the annotated collection of publicly available gene sequences. By the end of 2013 there was 169,331,407 sequences stored in the GenBank, which represents a 17-fold increase in data since the sequencing of the human genome in 2000 and an about 280,000-fold increa ...
Early frameshift alleles of zebrafish tbx5a that fail to
... To generate putative tbx5a null alleles in zebrafish, we employed Cas9 ribonucleoprotein complex (RNP)-mediated mutagenesis using our established sgRNA[tbx5ccA] that targets the first coding exon (Fig. 1A)11. This sgRNA targets the coding sequence in the first coding exon downstream of the conserved ...
... To generate putative tbx5a null alleles in zebrafish, we employed Cas9 ribonucleoprotein complex (RNP)-mediated mutagenesis using our established sgRNA[tbx5ccA] that targets the first coding exon (Fig. 1A)11. This sgRNA targets the coding sequence in the first coding exon downstream of the conserved ...
Identification of a C-terminal Poly(A)-binding Protein (PABP)
... sufficient to confer viability to cells depleted of the normal PABP gene (11). In Xenopus oocytes, RRMs can stimulate translation when tethered to reporter mRNA (18). As to the C terminus, although it does not bind RNA, it enables PABP to multimerize on poly(A) (12). Recently it was shown to include ...
... sufficient to confer viability to cells depleted of the normal PABP gene (11). In Xenopus oocytes, RRMs can stimulate translation when tethered to reporter mRNA (18). As to the C terminus, although it does not bind RNA, it enables PABP to multimerize on poly(A) (12). Recently it was shown to include ...
The Influence of Anticodon–Codon Interactions and Modified Bases
... concentration resulting from a single tRNA gene. As discussed in the introduction, this is a reasonable approximation in species where concentrations have been measured. It has been shown that some tRNAs may be charged less efficiently than others with their appropriate amino acid (Elf et al. 2003). ...
... concentration resulting from a single tRNA gene. As discussed in the introduction, this is a reasonable approximation in species where concentrations have been measured. It has been shown that some tRNAs may be charged less efficiently than others with their appropriate amino acid (Elf et al. 2003). ...
Energy and cellular metabolism
... Energy Can Be Converted from One Form to Another Recall that a general definition of energy is the capacity to do work. Work always involves movement and therefore is associated with kinetic energy. Potential energy can also be used to perform work, but the potential energy must first be converted t ...
... Energy Can Be Converted from One Form to Another Recall that a general definition of energy is the capacity to do work. Work always involves movement and therefore is associated with kinetic energy. Potential energy can also be used to perform work, but the potential energy must first be converted t ...
Supplementary Information (doc 1628K)
... The Affymetrix Expression Console Program was used to examine the Affymetrix Gene Array quality control factors for all samples in a project. Two separate independent algorithms were used to normalize the data for all samples within a project. Data is normalized using the Robust Multi-Array Analysis ...
... The Affymetrix Expression Console Program was used to examine the Affymetrix Gene Array quality control factors for all samples in a project. Two separate independent algorithms were used to normalize the data for all samples within a project. Data is normalized using the Robust Multi-Array Analysis ...
TRIzol Reagent
... After homogenization (before addition of chloroform) samples can be stored at -70°C for at least 1 year. The homogenated samples can also sit at room temperature for several hours before chloroform is added. Stability of RNA in TRIzol for field samples: If the biological sample is efficiently lysed ...
... After homogenization (before addition of chloroform) samples can be stored at -70°C for at least 1 year. The homogenated samples can also sit at room temperature for several hours before chloroform is added. Stability of RNA in TRIzol for field samples: If the biological sample is efficiently lysed ...
Regulation of transcript encoding the 43K
... activity. Denervation causes a 10- to 100-fold increase in AChR mRNA and AChR protein (for review, see Anderson, 1987). These nonsynaptic AChRs are not clustered at high density, but rather are present at 20to 100-fold lower concentration than at the synapse. Although fluorescence and histochemical ...
... activity. Denervation causes a 10- to 100-fold increase in AChR mRNA and AChR protein (for review, see Anderson, 1987). These nonsynaptic AChRs are not clustered at high density, but rather are present at 20to 100-fold lower concentration than at the synapse. Although fluorescence and histochemical ...
Integrative Genome-wide Analysis of the Determinants of RNA
... Based on the splicing graph constructed with SplAdder, we extracted 184, 941 introns located in 15, 387 genes that were part of alternative splicing events. Of these introns, 160, 208 were confirmed by at least 10 spliced alignments in at least one of the samples from the KIRC, GEUVADIS or ENCODE se ...
... Based on the splicing graph constructed with SplAdder, we extracted 184, 941 introns located in 15, 387 genes that were part of alternative splicing events. Of these introns, 160, 208 were confirmed by at least 10 spliced alignments in at least one of the samples from the KIRC, GEUVADIS or ENCODE se ...
Full report
... These plots are drawn for all protein, RNA and DNA chains in the entry. The first graphic for a chain summarises the proportions of the various outlier classes displayed in the second graphic. The second graphic shows the sequence view annotated by issues in geometry and electron density. Residues a ...
... These plots are drawn for all protein, RNA and DNA chains in the entry. The first graphic for a chain summarises the proportions of the various outlier classes displayed in the second graphic. The second graphic shows the sequence view annotated by issues in geometry and electron density. Residues a ...
Chapter 8
... On the assembled ribosome, a tRNA carrying the first amino acid is paired with the start codon on the mRNA. The place where this first tRNA sits is called the P site. A tRNA carrying the second amino acid approaches. ...
... On the assembled ribosome, a tRNA carrying the first amino acid is paired with the start codon on the mRNA. The place where this first tRNA sits is called the P site. A tRNA carrying the second amino acid approaches. ...