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1. ELONGATION
1. ELONGATION

... added to the 5’ end of the transcript, linked by a triphosphate bond. Then an AAUAAA sequence near the 3’ end is recognized by an enzyme that cuts off the end of the RNA approximately 20 bases farther down. At this time a stretch of 150 to 200 adenine nucleotides called a poly(A) tail is added at th ...
TUTORIAL FIGURES: Basic Molecular Biology
TUTORIAL FIGURES: Basic Molecular Biology

... regions called exons and these regions are interrupted with intervening non-coding regions called introns (blue). During transcription the whole segment of DNA corresponding to the gene is copied to RNA. An RNA processing removes the introns and the exons are joined at the intron-exon junctions (bot ...
DNA Biology
DNA Biology

... where protein synthesis occurs Has sites to bind both mRNA and tRNA ...
Chapter 10 Nucleic Acids and Protein synthesis
Chapter 10 Nucleic Acids and Protein synthesis

... transcription by binding to “PROMOTER REGIONS” ( these regions have lots of A-T base pairs) on the DNA. Promoter Region – marks the beginning of the DNA portion that will be transcribed RNA polymerase attaches to 1 strand of unzipped DNA and begins pasting together complementary RNA nucleotides to f ...
Pre – AP Biology
Pre – AP Biology

File
File

... DNA is transcribed and mRNA is translated; transcription produces RNA and translation produces polypeptides / protein; RNA polymerase for transcription and ribosomes for translation / ribosomes in translation only; transcription in the nucleus (of eukaryotes) and translation in the cytoplasm / at ER ...
Question How does DNA control a cell?By controlling Protein
Question How does DNA control a cell?By controlling Protein

... in changes in phenotype (17.5). ...
LECT35 trans1
LECT35 trans1

... Q: What is meant by Charging A: Charging means placing an amino acid on the 3’ (acceptor) end of the tRNA ...
RNA and Central Dogma
RNA and Central Dogma

SB2a Build DNA using the Nucleotides Then Print
SB2a Build DNA using the Nucleotides Then Print

... The scissors below represent the enzyme called DNA helicase. It is responsible for cutting the DNA molecule in half by breaking the hydrogen bonds between the bases so that transcription can occur. Copy and paste the nucleotides from a previous slide to show how transcription occurs. RNA nucleotide ...
Airgas template
Airgas template

... the cytoplasm, carrying the genetic code? a. Messenger RNA (mRNA) b. Transfer RNA (tRNA) c. Ribosomal RNA (rRNA) d. All of the above can move between the nucleus and the cytoplasm. ...
Targeted knock-up of endogenous genes using a
Targeted knock-up of endogenous genes using a

... increase the amount of protein made by a targeted endogenous gene. This technology was first demonstrated in an elegant study by Carrieri et al (Nature 491:454). This paper describes a particular lncRNA containing a SINEB2 repeat that increases the efficiency of protein translation for a target gene ...
Gene Activity - Haiku Learning
Gene Activity - Haiku Learning

... The Genetic Code The unit of a code consists of codons, each of which is a unique arrangement of symbols Each of the 20 amino acids found in proteins is uniquely specified by one or more codons  The symbols used by the genetic code are the mRNA bases - Function as “letters” of the genetic alphabet ...
Plant Molecular Biology
Plant Molecular Biology

... gene expression in living cells, found in bacteria and invertebrates CAT – chloramphenicol acetyltransferase, used as an early reporter in plants, assay with radioactive substrate, bacterial gene 26. (10 pts) What is the general phenotype of the Det/COP/Fus mutants of Arabidopsis? What is the role o ...
Mutations Website Assignment - Mercer Island School District
Mutations Website Assignment - Mercer Island School District

... 8. Being a carrier of the sickle cell anemia allele has a positive effect (which explains why this genetic disorder is most common among people who come from tropical areas have certain strains of mosquitoes.) Explain what this positive effect is. ...
Translation I
Translation I

... 5’UTR sometimes contains long GC-rich regions that tend to form secondary structure and inhibit ribosome scanning. This is found particularly in mRNAs for growth-promoting proteins (growthfactors, oncogene products) and is thought to be a regulatory element to prevent uncontrolled cell growth. Some ...
RNA:Structure, Function, Transcription, Translation
RNA:Structure, Function, Transcription, Translation

... sugar b. What shape does it form? ring c. How is ribose different from deoxyribose? one more oxygen ...
CHAPTER 7 From DNA to Protein
CHAPTER 7 From DNA to Protein

... Signals in DNA Tell RNA Polymerase Where to Start and Finish To begin transcription, RNA polymerase must be able to recognize the start of a gene and bind firmly to the DNA. The enzyme latches tightly onto the DNA once it encounters a region called PROMOTOR, which contains a sequence of nucleotides ...
Protein Synthesis
Protein Synthesis

... Ribosomal RNA (rRNA) • rRNA is a single strand 100 to 3000 nucleotides long • Globular in shape • Made inside the nucleus of a cell • Associates with proteins to form ribosomes • Site of protein Synthesis ...
Translation Tjian lec 26
Translation Tjian lec 26

... 1. Alternative RNA splicing: one mechanism evolved to expand diversity of gene products without increasing gene number 2. Control of alternative splicing by positive and negative splicing factors ( analogous to transcriptional activators and repressors) 3. Complexity of organisms reflected by exon n ...
AP BIO Unit 6 Review Ch. 14,15,16,18,19 Westbrook Gene
AP BIO Unit 6 Review Ch. 14,15,16,18,19 Westbrook Gene

... What must happen for transcription to be initiated? (many steps) Eukaryotes have regulatory proteins which have two distinct binding domains that allows for “control from a distance.” What are those binding domains called? What is the sequence of three tRNA nucleotides that is complementary to and b ...
Chapter 19 Nucleic Acids
Chapter 19 Nucleic Acids

... • In prokaryotes the primary mRNA transcript is translated directly • In eukaryotes transcription occurs in the nucleus, translation in the cytoplasm • Eukaryotic mRNA is processed in the nucleus without interfering with translation • In some mRNA, pieces are removed from the middle and the ends joi ...
Eukaryotic Gene Regulation
Eukaryotic Gene Regulation

... 1. For each of the following, is the statement true for prokaryotes or eukaryotes? a. Each gene is regulated separately by its own promoter b. DNA is not packed very extensively c. Regulatory proteins are made in the nucleus but act in the cytoplasm d. Less complex promoter structure with less regul ...
FINAL- CLICKER REVIEW
FINAL- CLICKER REVIEW

... E. Anaphase I ...
VII. Some methods for studying gene expression
VII. Some methods for studying gene expression

... the expression of a gene in a polycistronic mRNA can have secondary effects on the expression of downstream gene. i. The insertion of an transcription terminator prevents the transcription of downstream gene. ii. The mutation changing a codon to a nonsense codon will dissociate the ribosome from mRN ...
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