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REGULATION OF GENE EXPRESSION IN EUKARYOTES
REGULATION OF GENE EXPRESSION IN EUKARYOTES

... • GTFs only produce a basal level of transcription i.e. very low • Gene-specific factors (activators) are further required to regulate the activity of gene expression ...
Lecture 1
Lecture 1

... Transfer RNA (tRNA) is basically cloverleaf-shaped. tRNA carries the proper amino acid to the ribosome when the codons call for them. At the top of the large loop are three bases, the anticodon, which is the complement of the codon. There are 61 different tRNAs, each having a different binding site ...
Protein Synthesis Lab
Protein Synthesis Lab

... Click here to continue ...
Exploratorium Presentation
Exploratorium Presentation

... What is bacterial transformation? Transformation is the alteration of cells by the incorporation of foreign DNA into the cell ...
Molecular Genetics
Molecular Genetics

... – Promotor – Where RNA polymerase first binds. It is the region where transcription can occur so that proteins can be produced that allow the transport and break down of lactose. But these proteins are not needed unless lactose is present. – Operator – Like a light switch that turns transcription on ...
Making RNA in other ways
Making RNA in other ways

... • However some forms of retrotransposition may be responsible for the existence of introns • Introns may become copied into DNA or directly inserted into DNA as RNA – The insertion is sequence specific – So if cells fuse (as during fertilization) a retrotransposon can copy an intron from a gene on o ...
Lecture Three: Genes and Inheritance
Lecture Three: Genes and Inheritance

... The DNA Code: Every three “letters” on the DNA represents one amino acid. In the DNA double helix, A always binds to T and G always binds to C. PROBLEM: DNA is permanently stuck inside the nucleus. The ribosomes are outside the nucleus in the cytoplasm. How does the ribosome get the code? We need a ...
Homework 1 - Berkeley MCB
Homework 1 - Berkeley MCB

... average mRNA compare to the nuclear cycle times in the initial stages of fly development? For example, nuclear cycles 9 through 11 last no more than 6 minutes, while nuclear cycle 12 lasts about 10 minutes and cycle 13 on the order of 12 minutes. How do the genes that are actually expressed in this ...
STUDY GUIDE for MICROBIAL GENETICS 1. Define the following
STUDY GUIDE for MICROBIAL GENETICS 1. Define the following

... What is the Rolling Circle Model of bacterial DNA replication? In other words, describe the bidirectionality of bacterial DNA replication. Describe transcription and translation. a. What is the function of RNA Polymerase? b. In prokaryotic cells, where does transcription occur? In eukaryotic cells, ...
The abundance and cell cycle dependent expression of the mRNA
The abundance and cell cycle dependent expression of the mRNA

... obtained from the human samples was very strong (fig 2C). The relative content of HMG-17 mRNA in the two human samples was determined by scanning the autoradiograms and integrating the area under each peak. The data indicates that HeLa RNA contained approximately 6 times more HMG-17 mRNA than the RN ...
DNA - KK College of Nursing
DNA - KK College of Nursing

... DNA is also used as a long term storage device to store the genetic instructions ...
Transcription-Mediated Amplification
Transcription-Mediated Amplification

... Second level of specificity: An isothermal amplification utilizing specific oligonucleotides further increases specificity and assay sensitivity. Transcription-Mediated Amplification (TMA) is an isothermal molecular amplification process utilizing two enzymes, reverse transcriptase (RT) and RNA poly ...
Document
Document

... • Operon: a set of genes that are transcribed from the same promoter and controlled by the same operator site and regulatory proteins. • Regulon: a set of genes (and/or operons) expressed from separate promoter sites, but controlled by the same regulatory molecule. Global regulons may coordinate exp ...
Chapters 25-26 V2
Chapters 25-26 V2

... Figure 26.0 A painting of early Earth showing volcanic activity and photosynthetic prokaryotes ...
RNA Structure and the Versatility of RNA
RNA Structure and the Versatility of RNA

... • Large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence of the protein products of gene expression. Following transcription of primary transcript mRNA (known as pre-mRNA) by RNA polymerase, processed, mature mRNA is transla ...
DNA webquest
DNA webquest

... Click several more times, slowly, and study what happens. Repeat the animation and match the functions below. 5. Make a key of shapes for yourself using the colored pencils. Match the functions as you understand them from the animation. ____ Ligase ____ DNA Binding Proteins ____ ____ ____ ____ ...
If you have a the following genotypes as babies, what must the
If you have a the following genotypes as babies, what must the

... • Transcription happens first (how / where?) • Translation happens second (how / where?) • What is a codon? - a three nucleotide sequence that codes for a specific amino acid. ...
Thao_Molecular cell
Thao_Molecular cell

... other when present in the same cell. • Recessive  The one pair of allele that is masked by the other when present in the same cell and capable of producing its characteristics phenotype in the organism only when two alleles is present and identical. ...
Medical Genetics, Lecture 3
Medical Genetics, Lecture 3

... Synthesis of an RNA Transcript - Elongation • RNA polymerase synthesizes a single strand of RNA against the DNA template strand (anti-sense strand), adding nucleotides to the 3’ end of the RNA chain • As RNA polymerase moves along the DNA it continues to untwist the double helix, exposing about 10 ...
DNA paper 1 - DavidHein-CESRC-page
DNA paper 1 - DavidHein-CESRC-page

... information. It carries the information from the DNA is the nucleus to the cytosol. tRNA is transfer RNA. It is about 80 RNA nucleotides. It folds into a hairpin shape and binds to an amino acid to deliver to the ribosome. rRNA combines amino acids to form proteins. Transcription is the process wher ...
1. The non-living synthesis of simple organic molecules. 2. The
1. The non-living synthesis of simple organic molecules. 2. The

...  DNA and RNA can act as templates for copying  RNA self-replication does not require proteins/enzymes  Self-replicating molecules are subject to evolution by natural ...
Protein Metabolism - Orange Coast College
Protein Metabolism - Orange Coast College

... Hydrolysis of terminal peptidyl-tRNA bond Release of protein and last tRNA Dissociation of ribosome ...
Exam 2 review - Iowa State University
Exam 2 review - Iowa State University

... 37. A person’s STR may vary from another person’s STR because: A. The order of nucleotides B. The bases that are present C. The chromosomal location of the STR D. The number of times a sequence is repeated E. The number of coding regions 38. What is the function of the coding sequence? A. Determines ...
Analyzing Acclimation Response of
Analyzing Acclimation Response of

... • Analyzing common group of regulated genes between nutrient-limited cultures establishes a ‘core’ set of context-independent, regulated genes • This ‘core’ set of genes was analyzed and compared to previous studies to provide further data for cold temperature response of S. cerevisiae ...
1 Chapter 13: DNA, RNA, and Proteins Section 1: The Structure of
1 Chapter 13: DNA, RNA, and Proteins Section 1: The Structure of

... 3) mRNA carries instructions for making a protein from a gene and delivers them to the site of translation b. 1) during translation tRNA “reads” the mRNA sequence 2) translates the mRNA sequence into a specific sequence of protein subunits or amino acids 3) have amino acids attached to them 4) act a ...
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