Comparison of DNA Sequences with Protein Sequences
... to join some clearly related superfamilies (W.R.P, manuscript in preparation; the PIR39b database is available for downloading from ftp. virginia.edu:/pub/fasta). Two sequences from each of 46 families of proteins were used for these tests. The cDNA sequences, and their corresponding open reading fr ...
... to join some clearly related superfamilies (W.R.P, manuscript in preparation; the PIR39b database is available for downloading from ftp. virginia.edu:/pub/fasta). Two sequences from each of 46 families of proteins were used for these tests. The cDNA sequences, and their corresponding open reading fr ...
Real-Time PCR Probe Design
... How to Prepare Replicates •Pipet enough template into one tube for the number of replicates you plan to ...
... How to Prepare Replicates •Pipet enough template into one tube for the number of replicates you plan to ...
Electrophoresis may be defined as the movement of the particles of
... particular buffer medium, a particle’s mobility is determined by the net charge of the particle, the size and shape of the particle, and various other physical conditions. If enough time is allowed, the different components of materials are separated in the medium and can be discerned and analyzed. ...
... particular buffer medium, a particle’s mobility is determined by the net charge of the particle, the size and shape of the particle, and various other physical conditions. If enough time is allowed, the different components of materials are separated in the medium and can be discerned and analyzed. ...
The Play is the thing… - Biology Learning Center
... ribosome: giant machine (>200 proteins, 4 RNAs (2 > 1000 nucleotides) that oversees the reading of the mRNA and the creation of polypeptide aminoacyl tRNA synthetase: protein machine adds amino acid to tRNAs Termination factor: ‘reads’ UAA etc., => ribosome looses the peptide & ...
... ribosome: giant machine (>200 proteins, 4 RNAs (2 > 1000 nucleotides) that oversees the reading of the mRNA and the creation of polypeptide aminoacyl tRNA synthetase: protein machine adds amino acid to tRNAs Termination factor: ‘reads’ UAA etc., => ribosome looses the peptide & ...
Chapter 11 Regulation of Gene Expression
... from chromatin proteins during interphase, and histones remain associated with the DNA at many positions along the molecule even during transcription. The rate of transcription is therefore also controlled by the accessibility of DNA to RNApol and regulatory proteins. If the chromatin that contains ...
... from chromatin proteins during interphase, and histones remain associated with the DNA at many positions along the molecule even during transcription. The rate of transcription is therefore also controlled by the accessibility of DNA to RNApol and regulatory proteins. If the chromatin that contains ...
Analysis and nucleotide sequence of an origin of DNA replication in
... in the pBR322 vector portion, pWHI266 was partially digested with $au3A, ligated with the BamHl cleaved insertion sequence and transformed to E. coil RRI. Triangles indicate insertion sites of the BamHl insertion element and brackets the deleted Sau3A fragments.The insertion element is a 4.7-kb DNA ...
... in the pBR322 vector portion, pWHI266 was partially digested with $au3A, ligated with the BamHl cleaved insertion sequence and transformed to E. coil RRI. Triangles indicate insertion sites of the BamHl insertion element and brackets the deleted Sau3A fragments.The insertion element is a 4.7-kb DNA ...
Artificial White Blood Cell
... • Our engineered cells will “explode” when they detect a “bad” cell, killing both itself and the nearby bad cell. • The “explosion” is a sudden burst of H2O2, similar to a neutrophil’s oxidative burst. • The bad cell will be detected when it attempts to conjugate with our cell. This ensures it is ph ...
... • Our engineered cells will “explode” when they detect a “bad” cell, killing both itself and the nearby bad cell. • The “explosion” is a sudden burst of H2O2, similar to a neutrophil’s oxidative burst. • The bad cell will be detected when it attempts to conjugate with our cell. This ensures it is ph ...
MEDICAL BIOLOGY AND GENERAL GENETICS
... Peroxisomes are formed in ER. Their enzymes (oxidazes) oxidize amino acids with formation of peroxide (H2O2). Glyoxysomes are formed in Golgi complex, their enzymes transform fats into carbohydrates. Mitochondria have a shape of rods, filaments and granules. The sizes of mitochondria are from 0.5 to ...
... Peroxisomes are formed in ER. Their enzymes (oxidazes) oxidize amino acids with formation of peroxide (H2O2). Glyoxysomes are formed in Golgi complex, their enzymes transform fats into carbohydrates. Mitochondria have a shape of rods, filaments and granules. The sizes of mitochondria are from 0.5 to ...
Human Cytomegalovirus UL34 Early and late Proteins Are Essential
... There is no UL34 binding site within the major immediate early gene, and in studies utilizing transient expression assays, UL34 has no activating or repressing effect on the mIE promoter [9]. Consequently, the reduction in IE2 transcript levels seen in the absence of UL34 proteins suggests that UL34 ...
... There is no UL34 binding site within the major immediate early gene, and in studies utilizing transient expression assays, UL34 has no activating or repressing effect on the mIE promoter [9]. Consequently, the reduction in IE2 transcript levels seen in the absence of UL34 proteins suggests that UL34 ...
BIOLOGY - Learner
... be more complicated than the picture that Woese and his colleagues presented. The complication is lateral gene transfer, where individuals exchange genes between one another. Although not generally exhibited in Eukarya, mechanisms for lateral gene transfer (also known as “horizontal gene transfer”) ...
... be more complicated than the picture that Woese and his colleagues presented. The complication is lateral gene transfer, where individuals exchange genes between one another. Although not generally exhibited in Eukarya, mechanisms for lateral gene transfer (also known as “horizontal gene transfer”) ...
Discovery of MLL1 binding units, their localization to CpG Islands
... interphase stage of cell-cycle [31]. However, how MLL1 bookmarks genes to maintain transcriptional memory has not been addressed. The finding that gene-bookmarking by MLL1 is largely independent of the methylation status of H3K4 on mitotic chromosomes [31] provokes the question of whether interactio ...
... interphase stage of cell-cycle [31]. However, how MLL1 bookmarks genes to maintain transcriptional memory has not been addressed. The finding that gene-bookmarking by MLL1 is largely independent of the methylation status of H3K4 on mitotic chromosomes [31] provokes the question of whether interactio ...
Leukaemia Section t(10;11)(q22;q23) KMT2A/TET1 Atlas of Genetics and Cytogenetics in Oncology and Haematology
... transferase motif, a bromodomain; transcriptional regulatory factor involved in maintenance of Hox gene expression during embryogenesis and during the process of haematopoietic progenitors expansion and differentiation. ...
... transferase motif, a bromodomain; transcriptional regulatory factor involved in maintenance of Hox gene expression during embryogenesis and during the process of haematopoietic progenitors expansion and differentiation. ...
DNA barcoding parasite organisms found in terrestrial
... according to the manufacturer’s recommendations (Qiagen Inc., Valencia, CA). Prior to sequencing these DNA samples, a second PCR amplification was run containing 2 μL DNA sample to 5 μL Master Mix, comprised of 1 μL TAQ Buffer, 0.32 μL HCO or LCO, 2.68 μL H2O, and 1 μL Big Dye. The samples of the se ...
... according to the manufacturer’s recommendations (Qiagen Inc., Valencia, CA). Prior to sequencing these DNA samples, a second PCR amplification was run containing 2 μL DNA sample to 5 μL Master Mix, comprised of 1 μL TAQ Buffer, 0.32 μL HCO or LCO, 2.68 μL H2O, and 1 μL Big Dye. The samples of the se ...
3P Color Buffer
... 10X P-Green Buffer The 10X P-Green Buffer allows you to go directly from the thermal cycler to gel analysis. The buffer contains a compound that increases sample density, so that samples sink easily into the wells of an agarose gel. The 10X P-Green Buffer contains two dyes (yellow and blue) that sep ...
... 10X P-Green Buffer The 10X P-Green Buffer allows you to go directly from the thermal cycler to gel analysis. The buffer contains a compound that increases sample density, so that samples sink easily into the wells of an agarose gel. The 10X P-Green Buffer contains two dyes (yellow and blue) that sep ...
ISOLATE II PCR and Gel Kit
... membrane. The standard elution buffer volume is 15–30μl which is best for high DNA recovery and high DNA concentration for fragments <1000 bp. Large DNA fragments bind much more tightly and are much more difficult to elute than small DNA fragments. The ISOLATE II PCR and Gel Kit is recommended for D ...
... membrane. The standard elution buffer volume is 15–30μl which is best for high DNA recovery and high DNA concentration for fragments <1000 bp. Large DNA fragments bind much more tightly and are much more difficult to elute than small DNA fragments. The ISOLATE II PCR and Gel Kit is recommended for D ...
CS 753 BIOLOGY AND BIOCHEMISTRY
... is often required when studying productivity or fertility of soils. Sometimes the biomass of specific parts of the microbiota is required - for example fungal biomass versus bacterial biomass. If possible, the metdod should yield results comparable to the results used for plant and animal determinat ...
... is often required when studying productivity or fertility of soils. Sometimes the biomass of specific parts of the microbiota is required - for example fungal biomass versus bacterial biomass. If possible, the metdod should yield results comparable to the results used for plant and animal determinat ...
Isolation, Characterization, and Annotation: The Search for Novel
... their DNA into bacteria and cause lysis. As viruses, phages are not living, but they contain genetic material. Upon encountering a host bacterium, a phage attaches to the host, penetrates the cell membrane and injects its DNA. The genetic information can then follow two potential paths. In the lysog ...
... their DNA into bacteria and cause lysis. As viruses, phages are not living, but they contain genetic material. Upon encountering a host bacterium, a phage attaches to the host, penetrates the cell membrane and injects its DNA. The genetic information can then follow two potential paths. In the lysog ...
Supporting Materials S1..
... March 2009 reveals a distinct bacterial population actively assimilating DIC In March 2009 the proportion of the bacterial DIC-assimilating assemblage represented by Alphaproteobacteria, Bacteroidetes, and Gammaproteobacteria decreased. At that time, Alphaproteobacteria comprised approximately 45% o ...
... March 2009 reveals a distinct bacterial population actively assimilating DIC In March 2009 the proportion of the bacterial DIC-assimilating assemblage represented by Alphaproteobacteria, Bacteroidetes, and Gammaproteobacteria decreased. At that time, Alphaproteobacteria comprised approximately 45% o ...
Energetics of protein–DNA interactions
... Protein–DNA interactions are vital for many processes in living cells, especially transcriptional regulation and DNA modification. To further our understanding of these important processes on the microscopic level, it is necessary that theoretical models describe the macromolecular interaction energ ...
... Protein–DNA interactions are vital for many processes in living cells, especially transcriptional regulation and DNA modification. To further our understanding of these important processes on the microscopic level, it is necessary that theoretical models describe the macromolecular interaction energ ...
Chapter 15
... phenotype, there is a 50% chance that each daughter will be a carrier like her mother, and a 50% chance that each son will have the disorder. ...
... phenotype, there is a 50% chance that each daughter will be a carrier like her mother, and a 50% chance that each son will have the disorder. ...
Division 4.qxd
... by demonstrating that the gene had a fine structure consisting of a linear array of subelements. At the time Benzer began his classic work, the concept of the gene was different from what it is today. Genes were thought to be indivisible and to be the smallest units of recombination, mutation, and f ...
... by demonstrating that the gene had a fine structure consisting of a linear array of subelements. At the time Benzer began his classic work, the concept of the gene was different from what it is today. Genes were thought to be indivisible and to be the smallest units of recombination, mutation, and f ...
PDF
... could be because the evolution of eusociality entailed rapid evolution and specialisation of workers [39]. Alternatively, high intra-colony variability in worker phenotypes could be beneficial because it results in a more efficient workforce [40,41]. These factors could lead to increased recombinati ...
... could be because the evolution of eusociality entailed rapid evolution and specialisation of workers [39]. Alternatively, high intra-colony variability in worker phenotypes could be beneficial because it results in a more efficient workforce [40,41]. These factors could lead to increased recombinati ...
Computational Biophysics
... constants and chemical shifts were compared to experimentally derived values to establish a baseline which will be used as a starting point to develop semi-empirical models which may more accurately model theoretical simulations after experimental conditions. I also computationally investigated whet ...
... constants and chemical shifts were compared to experimentally derived values to establish a baseline which will be used as a starting point to develop semi-empirical models which may more accurately model theoretical simulations after experimental conditions. I also computationally investigated whet ...
Bruce Wallace Biotechnology Lab Program Student Guide 5th
... The plasmid pARA is 4058 base pairs (bp) in size. A “base pair” would be adenine:thymine or guanine:cytosine and is the common method used to express the size of DNA molecules. The plasmid carries the ampr gene, which encodes the protein beta lactamase, an enzyme that destroys the antibiotic ampicil ...
... The plasmid pARA is 4058 base pairs (bp) in size. A “base pair” would be adenine:thymine or guanine:cytosine and is the common method used to express the size of DNA molecules. The plasmid carries the ampr gene, which encodes the protein beta lactamase, an enzyme that destroys the antibiotic ampicil ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.