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Vocabulary 7
Vocabulary 7

... 1) DNA – made of subunits known as nucleotides – made of: • sugar • phosphate • base • Shape: Double Helix • Found in the nucleus; chromosomes ...
Slide 1
Slide 1

... into a plant cell? ...
A1985ATY5200001
A1985ATY5200001

... some of these ideas with one of my PhD oral examiners, John Fincham, at lunch after the examination! The models depended on the unwinding of two parental DNA molecules and the reciprocal annealing of single strands with complementary partners. When the hDNA in the model spanned a heterozygous site i ...
Who am I?
Who am I?

... What is cloning? Clones are identical copies of living things. Humans have cloned a lot of things already. ...
Site-specific recombination mechanisms exploit DNA
Site-specific recombination mechanisms exploit DNA

... Through a series of elegant genetic experiments in the early eighties Piet van de Putte (Laboratory of Molecular Genetics, Leiden University) determined that bacteriophage (Mu) changes its host range through expression of different tail fibers by changing the orientation of a specific DNA segment, t ...
5. Protein Synthesis
5. Protein Synthesis

... 5. Information flows from DNA to ________ to proteins. 6. What holds base pairs together? 7. What is the process of a cells making an exact copy of its DNA called? 8. What is a codon? 9. What is an anticodon and where is it found? 10. Briefly describe transcription. 11. Briefly describe translation. ...
WS 12 - Department of Chemistry | Oregon State University
WS 12 - Department of Chemistry | Oregon State University

... Why is dATP one of the four precursors of DNA, but dAMP is not? ...
All Living things pass on their genetic heritage by common processes.
All Living things pass on their genetic heritage by common processes.

... DNA is the genetic material 1. “One gene-one polypeptide” theory (see “one gene-one enzyme” theory). George Beadle and Edward Tatum (late 40’s to early 50’s) used X-rays to induce mutations in Neurospora crassa, which were unable to synthesize amino acid and vitamins. They traced the defect to the e ...
DNA Technology Study Guide Be able to identify and define these
DNA Technology Study Guide Be able to identify and define these

Project Title: Characterization of new genes mediating exchange of
Project Title: Characterization of new genes mediating exchange of

... experiments and many more during this past year. They screened over 100 genetic mutants that we previously found to be sensitive to killing by gamma radiation and chemical DNA damaging agents to identify which of them were specifically defective in DNA double-strand break repair. Rachel used the pla ...
The DNA Connection
The DNA Connection

... Genes and DNA A ...
Slide 1
Slide 1

... • mitochondria, chloroplast (the endosymbiont theory) • What form does DNA take in the nucleus? • chromosome • How do the 150 million base pairs that make up the human genome fit into the nucleus? • wrapped around histones • coiled and supercoiled chromatin condenses into chromosomes ...
Activity 4.4.1 Translating the DNA code
Activity 4.4.1 Translating the DNA code

... ...
The Human Genome Project CH 13 Sec 3 notes
The Human Genome Project CH 13 Sec 3 notes

... •Store large amount of information Steps in DNA Microarray •Use ________ from 2 different cells •Convert to complimentary _________________ •Label DNA with _________________________ –Ex: Green = normal, red = cancer •Place in microarray slide –incubate •Examine colors for gene expression –Yellow = s ...
Players in the protein game
Players in the protein game

... • Are tightly wound coils of DNA. Chromosomes can be seen in a light microscope but in order to see the DNA you have to have a high powered mircroscope ...
Genetic Engineering
Genetic Engineering

... DNA Fingerprinting Gel Electrophoresis separates pieces of DNA based on size (after being cut up with restriction enzymes) Different people will ...
Chapter 27 Bacteria
Chapter 27 Bacteria

... What was Frederick Griffith’s contribution to our understanding of DNA? (Refer back to Ch. 16) ...
Bacteria - sandsbiochem
Bacteria - sandsbiochem

... What was Frederick Griffith’s contribution to our understanding of DNA? (Refer back to Ch. 16) ...
Genetic Engineering
Genetic Engineering

... Leaves single stranded “sticky” ends that can become incorporated into other DNA sequences with COMPLIMENTARY BASES ...
notes for mondays lab
notes for mondays lab

... 1. Phosphate Buffered Saline (PBS): a salty solution of constant pH to keep tissues, cells, and proteins intact during maceration 2. Proteinase K: an enzyme that catalyzes the breakdown of cellular proteins by splitting them into smaller peptides and amino acids 3. Buffer AL: a cell lysis solution t ...
Microorganisms in Biotechnology
Microorganisms in Biotechnology

... depositing the new gene in the chromosome of that cell • The gene is then passed on to daughter cells as the cell divides ...
Chem*4570 Applied Biochemistry Lecture 11 Conjugation and
Chem*4570 Applied Biochemistry Lecture 11 Conjugation and

... Recombination is the process whereby sequences from one DNA molecule can exchange with sequences in another molecule. Homologous recombination may occur where where there are regions of sequnce match between the incoming and the target DNA. Incoming DNA may be DNA transferred by conjugation, by tran ...
DNA-notes
DNA-notes

... nucleus of a cell  *Genes within the chromosomes are considered the basic unit of heredity (Each gene has its own specific location called a LOCUS)  *Double stranded, double helix shape ...
Transformation
Transformation

... Pathogenic strain (smooth)- polysaccharide coating Non-pathogenic (rough) ...
Mendel`s work
Mendel`s work

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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