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Gene Therapy
Gene Therapy

... 4. Gene Therapy  is the replacement of defective genes ...
The Structure of DNA
The Structure of DNA

... • Maurice Wilkins and Rosalind Franklin used Xray crystallography to study the structure of DNA. – In this technique, X-rays are diffracted as they passed through aligned fibers of purified DNA. – The diffraction pattern can be used to deduce the three-dimensional shape of molecules. • James Watson ...
LOCALIZATION OF A MOLECULE
LOCALIZATION OF A MOLECULE

... IMMUNOLOCALIZATION • ANTIBODIES: what are they? what do they look like? where in the antibody does the specificity come from? - EPITOPES: what are they? what do they look like? ...
How DNA Determines Traits - Liberty Union High School District
How DNA Determines Traits - Liberty Union High School District

Protein Synthesis Word Scramble
Protein Synthesis Word Scramble

... Translate the DNA strand in your notebook What does translate mean? Read message and create new message! mRNA to Protein! (the whole goal of PROTEIN synthesis!) ...
forensic science
forensic science

... 1. The enzyme helicase, in DNA breaks the hydrogen bonds between the nitrogen bases that hold the two strands together, unzipping the DNA molecule. 2. As the DNA continues to unzip, free nucleotides from the surroundings in the nucleus bond to the single strands base pairing by DNA polymerase. 3. Th ...
DNA and Protein Synthesis
DNA and Protein Synthesis

... copy of your DNA. Why, then, are some cells nerve cells with dendrites and axons, while others are red blood cells that have lost their nuclei and are packed with hemoglobin? Why are cells so different in structure and function? If the characteristics of a cell depend upon the proteins that are synt ...
LYMPHOCYTE DEVELOPMENT and the REARRANGEMENT and
LYMPHOCYTE DEVELOPMENT and the REARRANGEMENT and

Worksheet for videos below
Worksheet for videos below

... DNA Replication 1. During which cell cycle phase do eukaryotes copy their DNA? ____________________________________ 2. Which theory of DNA replication is the correct theory as determined by the Meselson-Stahl experiment? _______________________________________________________________________________ ...
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Generation of diversity in lymphocyte antigen receptors

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Genetic Engineering
Genetic Engineering

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DNA Extraction from Human Cheek Cells
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Study Guide Genetics Final 2014
Study Guide Genetics Final 2014

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Genetics - Mr. Coleman's Biology

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... Multiple alleles – more than two alleles for a gene are found within a population. Polygenic traits – many genes contribute to a phenotype. ...
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Questions - Vanier College

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Protein Synthesis Notes Review
Protein Synthesis Notes Review

... 2. To make proteins, what does the DNA have to be decoded into? 3. What are the three parts that make up a RNA nucleotide? 4. What are the three differences between DNA and RNA? 5. If a DNA chain had the following sequence, CCGTAATAGCAT, what RNA nucleotides would attach to this sequence? 6. What is ...
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... DNA and amino acid sequences are forms of “chemical” or molecular evidence. Similar skeletal structures and organs are considered “anatomical” forms of evidence (homologous anatomy). Fossil evidence ...
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... 2g) The possible genetic makeup of a zygote can be predicted using Punnett Squares to determine possible allele combinations and their probabilities. 3a) A zygote’s phenotype is determined by its genotype, which is established during fertilization. Dominant alleles (A)can hide the presence of reces ...
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3.5 Genetic modification and biotechnology

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Notes: Introduction to Single Nucleotide Polymorphisms (SNPs)

... What about the nucleotides nearby CG dinucleotides? Conceivably, the effort of the cell to detect and repair CG-induced mutations may lead to errors in nearby bases. This would be the case if repair involved a process that required the extra DNA replication in the region of the dinucleotide. Some ty ...
Studying and Manipulating Genomes
Studying and Manipulating Genomes

... recombinant DNA in 1972  Fused fragments of DNA from one species into the genetic material from another  Allowed them to isolate and replicate subsets of DNA from any organism ...
Self-Assembly at nano-Scale Binary Nanoparticles Superlattices
Self-Assembly at nano-Scale Binary Nanoparticles Superlattices

... before addition of secondary antibodies bound to DNA:Au nanoparticle conjugates. • Unreacted Au nanoparticle conjugates are removed after magnetic separation, then elevated temperature release the barcode DNA for analysis. • Each Au nanosphere carries hundreds of identical barcode DNA strands, provi ...
evaluation of a one-step dna extraction method for “touch”
evaluation of a one-step dna extraction method for “touch”

... Due to advances in DNA typing technologies, it is possible to generate a DNA profile from touched objects or trace amounts of biological material (< 100pg). Therefore, it is important to ensure that sample collection and DNA purification methods recover the maximal amount of DNA from each sample. Th ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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