Identification of the Minus-Dominance Gene Ortholog in
... Full-length cDNA synthesis from the total RNA was carried out with the CapFishing full-length cDNA premix kit (Seegene, Seoul, Korea). Nested RT–PCR using this cDNA yielded the partial fragment of GpMID; the primers used in the first PCR were the Seegene kit’s 39-rapid amplification of the cDNA end ...
... Full-length cDNA synthesis from the total RNA was carried out with the CapFishing full-length cDNA premix kit (Seegene, Seoul, Korea). Nested RT–PCR using this cDNA yielded the partial fragment of GpMID; the primers used in the first PCR were the Seegene kit’s 39-rapid amplification of the cDNA end ...
Biocatalytic potential of thermophilic bacteria and actinomycetes
... The polymerase chain reaction (PCR) process has led to a huge advance in molecular biology. Developments in this process have been to a large extent facilitated by the availability of thermostable DNA polymerases, which catalyze the elongation of the primer DNA strand [69]. Taq polymerase from Therm ...
... The polymerase chain reaction (PCR) process has led to a huge advance in molecular biology. Developments in this process have been to a large extent facilitated by the availability of thermostable DNA polymerases, which catalyze the elongation of the primer DNA strand [69]. Taq polymerase from Therm ...
2000 Genome Biology paper
... large-scale genome-changing events were studied through genetic or cytological studies. With the availability of many complete genome sequences it is now possible to study such events through comparative genomics. The publication of the yeast genome has led to much better insight into the duplicatio ...
... large-scale genome-changing events were studied through genetic or cytological studies. With the availability of many complete genome sequences it is now possible to study such events through comparative genomics. The publication of the yeast genome has led to much better insight into the duplicatio ...
Glycolysis, Krebs Cycle, Electron Transport Chain
... 4. How are the accuracy of DNA and mRNA codes assured? Enzymes such as DNA and RNA polymerase check for the correct bonding pattern. ...
... 4. How are the accuracy of DNA and mRNA codes assured? Enzymes such as DNA and RNA polymerase check for the correct bonding pattern. ...
Application for rDNA Review/Registration for
... RECOMBINANT DNA Please identify the type of experiment described in this application/registration form by checking the appropriate box(s) below. ...
... RECOMBINANT DNA Please identify the type of experiment described in this application/registration form by checking the appropriate box(s) below. ...
Gene Section JUN (V-Jun sarcoma virus 17 oncogene homolog (avian))
... deficiency of Jun gene causes massive hepatocyte apoptosis. The potential oncogenic transformation of JUN has been revealed by overexpression experiments. ...
... deficiency of Jun gene causes massive hepatocyte apoptosis. The potential oncogenic transformation of JUN has been revealed by overexpression experiments. ...
Gill: Gene Regulation II
... • DNA can be bent into 3D shape preventing enhancer – promoter interactions. • Activator and co-activator proteins can be modified into inactive states. Note: repressor thus can relate to specific DNA sequences or proteins. http://cs273a.stanford.edu [Bejerano Fall16/17] ...
... • DNA can be bent into 3D shape preventing enhancer – promoter interactions. • Activator and co-activator proteins can be modified into inactive states. Note: repressor thus can relate to specific DNA sequences or proteins. http://cs273a.stanford.edu [Bejerano Fall16/17] ...
iGenetics: A Molecular Approach, 3e (Russell/Bose)
... 43) The nucleus separates the chromosomes from the rest of the cell. How is the information in the DNA communicated to the cell? Answer: The nuclear membrane has pores and is permeable to certain molecules. This allows selected materials, like RNA and proteins, to move in and out of the nucleus. Ski ...
... 43) The nucleus separates the chromosomes from the rest of the cell. How is the information in the DNA communicated to the cell? Answer: The nuclear membrane has pores and is permeable to certain molecules. This allows selected materials, like RNA and proteins, to move in and out of the nucleus. Ski ...
The evolution of sex chromosomes: similarities and differences
... Z chromosomes of both taxa share several markers Thus they probably had the same ancestral sex chromosome Recombination has been suppressed only in the chicken lineage (including other neognathae), and not in palaeognathous birds ...
... Z chromosomes of both taxa share several markers Thus they probably had the same ancestral sex chromosome Recombination has been suppressed only in the chicken lineage (including other neognathae), and not in palaeognathous birds ...
this PDF file
... acetate as its sole carbon source or photo autotrophically using atmospheric carbon. Its haplontic life cycle has a quick replication time of 8-10 hours and its genome is completely sequenced. In contrast to angiosperms, Chlamydomonas also has two pathways for chlorophyll (Chl) biosynthesis, which i ...
... acetate as its sole carbon source or photo autotrophically using atmospheric carbon. Its haplontic life cycle has a quick replication time of 8-10 hours and its genome is completely sequenced. In contrast to angiosperms, Chlamydomonas also has two pathways for chlorophyll (Chl) biosynthesis, which i ...
Chapter 29 Slides
... • RecBCD initiates recombination in E.coli • RecA forms nucleoprotein filament for strand invasion and homologous pairing • RuvA, RuvB, RuvC drive branch migration and process the Holliday junction into recombination products • Eukaryotic systems are probably similar ...
... • RecBCD initiates recombination in E.coli • RecA forms nucleoprotein filament for strand invasion and homologous pairing • RuvA, RuvB, RuvC drive branch migration and process the Holliday junction into recombination products • Eukaryotic systems are probably similar ...
Epigenetic Inactivation of Chalcone Synthase-A
... of DNA methylation in the affected promoters, as well as changes in histone modification. The siRNAs may also direct DNA methylation in the nucleus (for a review, see Matzke et al. 2004). Like siRNAs, small RNAs called micro RNAs also negatively regulate the expression of endogenous genes through ei ...
... of DNA methylation in the affected promoters, as well as changes in histone modification. The siRNAs may also direct DNA methylation in the nucleus (for a review, see Matzke et al. 2004). Like siRNAs, small RNAs called micro RNAs also negatively regulate the expression of endogenous genes through ei ...
iGenetics: A Molecular Approach, 3e (Russell/Bose)
... 45) For geneticists, why is it important that genetic variability exist in the population under study? Answer: Genetic variation in individuals of a population is important for studying the inheritance pattern of those characteristics. If all the members of a population were identical for the trait ...
... 45) For geneticists, why is it important that genetic variability exist in the population under study? Answer: Genetic variation in individuals of a population is important for studying the inheritance pattern of those characteristics. If all the members of a population were identical for the trait ...
Lecture-Mic 623-Plasmids-Listeria - Home
... (pLSV1) was constructed which carried the temperature-sensitive gram-positive replication origin from plasmid pTV32(Ts). ...
... (pLSV1) was constructed which carried the temperature-sensitive gram-positive replication origin from plasmid pTV32(Ts). ...
Aalborg Universitet Characterization of Lipoxygenases from Potato Tuber (cv. kuras)
... The three Lox isoforms from leaf plus leaf and root are longer at their N-termini, corresponding to chloroplast targeting signals. Tuber StLoxs are found in vacuoles, but the transport mechanism is unknown due to no known targeting signal. Tuber Loxs are more closely related to the pathogen-induced ...
... The three Lox isoforms from leaf plus leaf and root are longer at their N-termini, corresponding to chloroplast targeting signals. Tuber StLoxs are found in vacuoles, but the transport mechanism is unknown due to no known targeting signal. Tuber Loxs are more closely related to the pathogen-induced ...
Genome organization of Magnaporthe grisea
... fungus Magnaporthe grisea (Guy11]2539) was constructed by adding 87 cosmid-derived RFLP markers to previously generated maps. The new map consists of 203 markers representing 132 independently segregating loci and spans approximately 900 cM with an average resolution of 4.5 cM. Mapping of 33 cosmid ...
... fungus Magnaporthe grisea (Guy11]2539) was constructed by adding 87 cosmid-derived RFLP markers to previously generated maps. The new map consists of 203 markers representing 132 independently segregating loci and spans approximately 900 cM with an average resolution of 4.5 cM. Mapping of 33 cosmid ...
Gene Conversion as a Source of Nucleotide Diversity in
... falcipain A and B are both AAA, but isolate HB3 has codon AGA (empty oval in figure 1). The possibility that PCR artifacts account for the sequence polymorphisms in figure 1 can be ruled out for three reasons. First, the primers are specific to the divergent flanking sequences and hence specific to ...
... falcipain A and B are both AAA, but isolate HB3 has codon AGA (empty oval in figure 1). The possibility that PCR artifacts account for the sequence polymorphisms in figure 1 can be ruled out for three reasons. First, the primers are specific to the divergent flanking sequences and hence specific to ...
Contig annotation tool CAT robustly classifies assembled
... bioRxiv preprint first posted online Sep. 1, 2016; doi: http://dx.doi.org/10.1101/072868. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
... bioRxiv preprint first posted online Sep. 1, 2016; doi: http://dx.doi.org/10.1101/072868. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
Chapter 18
... Chapter 18 - Genetics of Viruses and Bacteria NEW AIM: Viruses: Packaged Genes… Influenza looks different…it has an envelope. What’s up with ...
... Chapter 18 - Genetics of Viruses and Bacteria NEW AIM: Viruses: Packaged Genes… Influenza looks different…it has an envelope. What’s up with ...
Plasma Nucleic Acids in the Diagnosis and Management
... diagnosis. Approximately one-fourth of patients with colorectal cancer and one-half of those with pancreatic cancer have mutant K-ras sequences detectable in plasma. If the analysis is confined to those in whom the relevant mutation is confirmed in the tissue, in both instances approximately one-hal ...
... diagnosis. Approximately one-fourth of patients with colorectal cancer and one-half of those with pancreatic cancer have mutant K-ras sequences detectable in plasma. If the analysis is confined to those in whom the relevant mutation is confirmed in the tissue, in both instances approximately one-hal ...
5 The Genetics of Bacteria and Their Viruses
... Answer: The high rate of integration and the preference for the same site originally occupied by the F factor suggest that the F´ contains some homology with the original site. The source of homology could be a fragment of the F factor, or more likely, it is homology with the chromosomal copy of the ...
... Answer: The high rate of integration and the preference for the same site originally occupied by the F factor suggest that the F´ contains some homology with the original site. The source of homology could be a fragment of the F factor, or more likely, it is homology with the chromosomal copy of the ...
SUMMARY The steady state kinetics of initiation of T7 DNA transcrip
... Nucleic Acids Research schema it is possible that one of the routes if favored under normal conditions, but all of them are principally possible. This would be called a kinetically ordered mechanism. One could also think about "mixed-type" mechanisms, in which substrates bind randomly, while produc ...
... Nucleic Acids Research schema it is possible that one of the routes if favored under normal conditions, but all of them are principally possible. This would be called a kinetically ordered mechanism. One could also think about "mixed-type" mechanisms, in which substrates bind randomly, while produc ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.