Bacterial community composition in the rhizosphere of a transgenic
... can vary dramatically depending on the chromosomal insertion site [17] and other genes, e.g. those in proximity to the insertion site, may also be a¡ected. Should such unintended e¡ects have any impact on soil microbial activity, it would likely be seen ¢rst in the rhizosphere. The composition of mi ...
... can vary dramatically depending on the chromosomal insertion site [17] and other genes, e.g. those in proximity to the insertion site, may also be a¡ected. Should such unintended e¡ects have any impact on soil microbial activity, it would likely be seen ¢rst in the rhizosphere. The composition of mi ...
1_Standards for the Safety Assessment of Genetically Modified Foods (Seed Plants).pdf
... produced in the recombinant plant, or to synthesize or produce the substance from an alternative source. In this case, the material should be shown to be biochemically, structurally, and functionally equivalent to that produced in the recombinant plant. The safety assessment of the currently used an ...
... produced in the recombinant plant, or to synthesize or produce the substance from an alternative source. In this case, the material should be shown to be biochemically, structurally, and functionally equivalent to that produced in the recombinant plant. The safety assessment of the currently used an ...
Table S2
... Table S2, continued Pds1: Inhibits the onset of anaphase by binding and sequestering the Esp1 protease that cleaves the cohesin complexes that hold sister chromatids together. Binding of Pds1 to Esp1 was reported to depend in Cdc28 phosphorylation[53] Sic1: Inhibitor of Clb-Cdc28. Phosphorylation o ...
... Table S2, continued Pds1: Inhibits the onset of anaphase by binding and sequestering the Esp1 protease that cleaves the cohesin complexes that hold sister chromatids together. Binding of Pds1 to Esp1 was reported to depend in Cdc28 phosphorylation[53] Sic1: Inhibitor of Clb-Cdc28. Phosphorylation o ...
Using the Basic Local Alignment Search Tool (BLAST) - bio-bio-1
... word matches using log odds scores in the BLOSUM62 amino acid substitution matrix. For the BLAST algorithm, the word length is fixed at 3 (formerly 4) for proteins and 11 for nucleic acids (three if the sequences are translated in all six reading frames). These lengths are the minimum needed to achi ...
... word matches using log odds scores in the BLOSUM62 amino acid substitution matrix. For the BLAST algorithm, the word length is fixed at 3 (formerly 4) for proteins and 11 for nucleic acids (three if the sequences are translated in all six reading frames). These lengths are the minimum needed to achi ...
A Symbolic and Graphical Gene Regulation Model of the lac Operon
... Biological research has changed drastically over the last decade. Nowadays, in an effort to minimize the time spent in the laboratory, data gathering and analysis is done primaily on computers. This has heralded new interdisciplinary fields between biology and computer science. Biological computing ...
... Biological research has changed drastically over the last decade. Nowadays, in an effort to minimize the time spent in the laboratory, data gathering and analysis is done primaily on computers. This has heralded new interdisciplinary fields between biology and computer science. Biological computing ...
Sperm Cell in ART
... male infertility problems. In general we can say that traditional semen parameters provide a limited degree of diagnostic information, thus we are aware that these indexes of diagnosis should be revisited, which includes more specific test of sperm assessments, such as DNA tests and sperm proteome. ...
... male infertility problems. In general we can say that traditional semen parameters provide a limited degree of diagnostic information, thus we are aware that these indexes of diagnosis should be revisited, which includes more specific test of sperm assessments, such as DNA tests and sperm proteome. ...
22 August 2002
... the two amino-acid substitutions specific to humans in exon 7. Individuals with disruption of FOXP2 have multiple difficulties with both expressive and receptive aspects of language and grammar, and the nature of the core deficit remains a matter of debate18-20. Nevertheless, a predominant feature o ...
... the two amino-acid substitutions specific to humans in exon 7. Individuals with disruption of FOXP2 have multiple difficulties with both expressive and receptive aspects of language and grammar, and the nature of the core deficit remains a matter of debate18-20. Nevertheless, a predominant feature o ...
Table 7. Summary statistics for the consensus gene set of Haliotis
... 5.31 and 2.02 times larger than genomes size of L.gigantea (0.35 Gb) and A.californica (0.92 Gb) in the same Gastropoda class. In animals, the increase of genome size is commonly driven by transposable element, and this is a known genetic adaption mechanism to stressful environments[22]. Therefore, ...
... 5.31 and 2.02 times larger than genomes size of L.gigantea (0.35 Gb) and A.californica (0.92 Gb) in the same Gastropoda class. In animals, the increase of genome size is commonly driven by transposable element, and this is a known genetic adaption mechanism to stressful environments[22]. Therefore, ...
'This day designing God Hath put into my hand
... erythrocytic parasite including malarial mitogens, toxic proteins, prostaglandins
... erythrocytic parasite including malarial mitogens, toxic proteins, prostaglandins
Milestone2
... Many eukaryotic genes contain a DNA sequence, called a TATA box, in their promoter region, i.e., just upstream of their start codon. The TATA box is so named because it often consists of the following hexamer (i.e., 6 nucleotide) motif: “TATAAA”. Write a Python program named TATA.py, which reads in ...
... Many eukaryotic genes contain a DNA sequence, called a TATA box, in their promoter region, i.e., just upstream of their start codon. The TATA box is so named because it often consists of the following hexamer (i.e., 6 nucleotide) motif: “TATAAA”. Write a Python program named TATA.py, which reads in ...
Reconstructing evolution: Gene transfer from plastids to the nucleus
... revealed that the genomes of plastids have been greatly diminished compared with any possible free-living ancestor. The plastid genome was shown to be far too small to encode the proteome of the organelle and it was deduced that the control of plastid biogenesis and function is massively dependent u ...
... revealed that the genomes of plastids have been greatly diminished compared with any possible free-living ancestor. The plastid genome was shown to be far too small to encode the proteome of the organelle and it was deduced that the control of plastid biogenesis and function is massively dependent u ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... determinant from an R-factor.--Unlike P22Tc-10, however, P22pro-2 and P22pro-3 fail to integrate normally during lysogenizing infections, even when provided with all known integration functions. These results suggest that the proline substitutions have created a defect in the phage attachment site a ...
... determinant from an R-factor.--Unlike P22Tc-10, however, P22pro-2 and P22pro-3 fail to integrate normally during lysogenizing infections, even when provided with all known integration functions. These results suggest that the proline substitutions have created a defect in the phage attachment site a ...
Transcription
... - in chloroplasts: both phage and eubacterial type) • TBP required for promoter recruitment in all 3 ...
... - in chloroplasts: both phage and eubacterial type) • TBP required for promoter recruitment in all 3 ...
Part III: Laboratory – Electrophoresis
... 1. Obtain an Arabidopsis plant and observe and record its phenotype. Take a piece of leaf tissue that is approximately an eighth of an inch in diameter. If the leaves are too small, take tissue from multiple leaves (from the same plant) until you have the equivalent amount of leaf tissue. Note: Plan ...
... 1. Obtain an Arabidopsis plant and observe and record its phenotype. Take a piece of leaf tissue that is approximately an eighth of an inch in diameter. If the leaves are too small, take tissue from multiple leaves (from the same plant) until you have the equivalent amount of leaf tissue. Note: Plan ...
Precise insertion and guided editing of higher plant
... bioRxiv preprint first posted online Feb. 20, 2017; doi: http://dx.doi.org/10.1101/109983. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license. ...
... bioRxiv preprint first posted online Feb. 20, 2017; doi: http://dx.doi.org/10.1101/109983. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license. ...
1548 Tn Gene Is Borne by Composite Transposon Aminoglycoside
... 92296 Châtenay-Malabry,2 France Received 13 January 2005/Returned for modification 25 February 2005/Accepted 23 March 2005 ...
... 92296 Châtenay-Malabry,2 France Received 13 January 2005/Returned for modification 25 February 2005/Accepted 23 March 2005 ...
Cloning, expression, sequence analysis and
... IdentiJication of a Bacillus subtilis 168 autolysin structural gene. A Agtl 1 library containing a partial EcoRI digest of B. subtilis 168 trpC2 genomic DNA (gift from Dr C. Price, University of California, Davis, USA) was used to screen for autolysin activity. E. coli ER1458 was infected with phage ...
... IdentiJication of a Bacillus subtilis 168 autolysin structural gene. A Agtl 1 library containing a partial EcoRI digest of B. subtilis 168 trpC2 genomic DNA (gift from Dr C. Price, University of California, Davis, USA) was used to screen for autolysin activity. E. coli ER1458 was infected with phage ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... 0.1 gin. gelatin, and HC1 to adjust to pH 7.4. Analytical Methods.--Most of the experiments required periodic measures of DNAP~ and mature phage P~. DNA-P 3~ was determined (Schmidt and Thannhauser, 1945) by precipitating a chilled 1 ml. sample of the culture in a conical tube with 9 ml. 0.3 M trich ...
... 0.1 gin. gelatin, and HC1 to adjust to pH 7.4. Analytical Methods.--Most of the experiments required periodic measures of DNAP~ and mature phage P~. DNA-P 3~ was determined (Schmidt and Thannhauser, 1945) by precipitating a chilled 1 ml. sample of the culture in a conical tube with 9 ml. 0.3 M trich ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.