UNIT 1: DNA and the Genome
... In order to synthesise (build) a protein, a copy of the information carried on the DNA (in the nucleus) must be made and transferred to a ribosome (in the cytoplasm). This is done by a process known as transcription, using an enzyme called RNA polymerase. ...
... In order to synthesise (build) a protein, a copy of the information carried on the DNA (in the nucleus) must be made and transferred to a ribosome (in the cytoplasm). This is done by a process known as transcription, using an enzyme called RNA polymerase. ...
Leukaemia Section t(3;11)(q28;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... A complex karyotype was found in the only available case, with +8 and other anomalies. ...
... A complex karyotype was found in the only available case, with +8 and other anomalies. ...
2.6-7 and 3.1-3 DNA and intro to Genetics
... $500 Answer 4.4 Biotechnology A small amount of a suspect’s DNA is obtained from a crime scene. What techniques would be used to carry out DNA profiling? C. Polymerase chain reaction (PCR) and gel electrophoresis ...
... $500 Answer 4.4 Biotechnology A small amount of a suspect’s DNA is obtained from a crime scene. What techniques would be used to carry out DNA profiling? C. Polymerase chain reaction (PCR) and gel electrophoresis ...
Enhancing and Evolving to “Perfection”? Unit Study Guid e PART I
... 5. Use the WORD BANK below to compare bacteria and viruses in the Venn diagram. Write each word or phrase within the area that shows whether the characteristic belongs to both, only bacteria, or only viruses (pages 320-321 and 200-203) WORD BANK: Can be killed by antibiotics Single-celled organisms ...
... 5. Use the WORD BANK below to compare bacteria and viruses in the Venn diagram. Write each word or phrase within the area that shows whether the characteristic belongs to both, only bacteria, or only viruses (pages 320-321 and 200-203) WORD BANK: Can be killed by antibiotics Single-celled organisms ...
DNA RESTRICTION ANALYSIS
... DNA fragments will migrate towards the + anode end. 7. Fill box with TAE buffer, to level that just covers entire surface of gel by about 2mm. 8. Make certain that sample wells left by comb are completely submerged by buffer. 9. The gel is now ready to load with DNA. Loading gel with DNA 1. Your tab ...
... DNA fragments will migrate towards the + anode end. 7. Fill box with TAE buffer, to level that just covers entire surface of gel by about 2mm. 8. Make certain that sample wells left by comb are completely submerged by buffer. 9. The gel is now ready to load with DNA. Loading gel with DNA 1. Your tab ...
Interaction of a Nuclear Protein with 5` Flanking Region of
... To determine whether the two distinct fragments 4 and 8' share a common DNA-binding protein, competit~ve binding assays were carried out, where radiolabeled DNA fragment 4 was reacted with endosperm nuclea r proteins in the presence of increasing amounts of cold DNA fragments 4 and 8' as competitors ...
... To determine whether the two distinct fragments 4 and 8' share a common DNA-binding protein, competit~ve binding assays were carried out, where radiolabeled DNA fragment 4 was reacted with endosperm nuclea r proteins in the presence of increasing amounts of cold DNA fragments 4 and 8' as competitors ...
Protein Synthesis
... The Polypeptide chain is put together. Ribosome's moves along the chain and decodes the mRNA and attaches the amino acids together by peptide bonds. The tRNA will bring in the next amino acid, pair up with the codon on the mRNA and attach the next amino acid together ...
... The Polypeptide chain is put together. Ribosome's moves along the chain and decodes the mRNA and attaches the amino acids together by peptide bonds. The tRNA will bring in the next amino acid, pair up with the codon on the mRNA and attach the next amino acid together ...
Homework 1 - Berkeley MCB
... 300 amino acids, there will be a huge number of different possible sequences. (a) How many different sequences are there for a 300 amino acid protein? But that wasn’t the provocative remark. The provocative remark was that if we took only one molecule of each of these different possible proteins, it ...
... 300 amino acids, there will be a huge number of different possible sequences. (a) How many different sequences are there for a 300 amino acid protein? But that wasn’t the provocative remark. The provocative remark was that if we took only one molecule of each of these different possible proteins, it ...
DNA Structure and Function
... Sequence of nucleotide pairs dictates the sequence of amino acids in the protein specified by the gene. o The genetic material must be able to change. Watson and Crick discovered the double helix model through “model building” (assembly of previous and ongoing experiments) process in 1953. Rosalin ...
... Sequence of nucleotide pairs dictates the sequence of amino acids in the protein specified by the gene. o The genetic material must be able to change. Watson and Crick discovered the double helix model through “model building” (assembly of previous and ongoing experiments) process in 1953. Rosalin ...
It`s Alive! ...Or Not!
... • Carbohydrates come in two basic forms: complex and simple. • Simple carbs are one, two, or at most three units of sugar linked together in single molecules. • Complex carbs are hundreds or thousands of sugar units linked together in single molecules. • Simple sugars are easily identified by their ...
... • Carbohydrates come in two basic forms: complex and simple. • Simple carbs are one, two, or at most three units of sugar linked together in single molecules. • Complex carbs are hundreds or thousands of sugar units linked together in single molecules. • Simple sugars are easily identified by their ...
Transcription and Translation
... regulatory gene is not transcribed but has control elements, one of which is the promoter. A promoter is unique to each gene. • There is always a sequence of bases on the DNA strand called an initiation signal. • Promoters also contain consensus sequences, such as the TATA box, in which the two nucl ...
... regulatory gene is not transcribed but has control elements, one of which is the promoter. A promoter is unique to each gene. • There is always a sequence of bases on the DNA strand called an initiation signal. • Promoters also contain consensus sequences, such as the TATA box, in which the two nucl ...
The Big Picture: an outline of the concepts covered to date
... A. Each diploid individual contains two copies of a given gene B. Each Gene can have different forms called alleles. There are two alleles in a diploid individual The form that is expressed phenotypically in the heterozygote is known as the dominant allele. It is an operational definition C. These c ...
... A. Each diploid individual contains two copies of a given gene B. Each Gene can have different forms called alleles. There are two alleles in a diploid individual The form that is expressed phenotypically in the heterozygote is known as the dominant allele. It is an operational definition C. These c ...
Repressing Integrase attachment site operation
... extracted from the host’s genome [1]–[3]. These enzymes are particularly interesting and useful to synthetic biologists because they are inherently directional. A serine integrase tetramer will catalyze a recombination event between two heterotypic DNA “attachment” sites (attP and attB) [4]. Without ...
... extracted from the host’s genome [1]–[3]. These enzymes are particularly interesting and useful to synthetic biologists because they are inherently directional. A serine integrase tetramer will catalyze a recombination event between two heterotypic DNA “attachment” sites (attP and attB) [4]. Without ...
Library screening
... genomic clone, use of PCR product to isolate cDNA or genomic clones, use of a partial gene or cDNA sequence to isolate a full-length sequence Identification of closely related gene in a gene family Isolation of related genes from other species Isolation of genes encoding proteins which have been com ...
... genomic clone, use of PCR product to isolate cDNA or genomic clones, use of a partial gene or cDNA sequence to isolate a full-length sequence Identification of closely related gene in a gene family Isolation of related genes from other species Isolation of genes encoding proteins which have been com ...
Meiosis Chapeter 11 section #4
... Chromosome Number •Homologous Different # for different species Chromosomes • Full set = 2N=Diploid are the sets of • N= pair •each # pairs • 1 pair from mother • 1 pair from father • Humans= 23 pairs or • 46 total ...
... Chromosome Number •Homologous Different # for different species Chromosomes • Full set = 2N=Diploid are the sets of • N= pair •each # pairs • 1 pair from mother • 1 pair from father • Humans= 23 pairs or • 46 total ...
Test-Questions to Lab Exam 1 on the Autumn Semester of 2015
... B. DNA polymerase enzyme C. Introns D. Maternal (template) chain E. RNA polymerase enzyme 45. An experiment proved that UV-irradiated skin cells of patients with xeroderma pigmentosum restore the native structure of DNA slower than the cells of healthy people due to the defect in repair enzyme. What ...
... B. DNA polymerase enzyme C. Introns D. Maternal (template) chain E. RNA polymerase enzyme 45. An experiment proved that UV-irradiated skin cells of patients with xeroderma pigmentosum restore the native structure of DNA slower than the cells of healthy people due to the defect in repair enzyme. What ...
Chapter 9
... The Meaning of the Names of Some Microorganisms • Escherichia coli: Named after Theodore Escherich in 1888; found in the colon • Entamoeba histolytica: Ent, intestinal; amoebae, shape/movement; histo, tissue; lytic, lysing or digesting tissue ...
... The Meaning of the Names of Some Microorganisms • Escherichia coli: Named after Theodore Escherich in 1888; found in the colon • Entamoeba histolytica: Ent, intestinal; amoebae, shape/movement; histo, tissue; lytic, lysing or digesting tissue ...
Transposons
... therefore there are few problems with homology to existing sequences in the chromosome; in contrast to most other transposons Mu does not need a separate vector system since it is itself a vector A wide variety of useful mutants of Mu have been ...
... therefore there are few problems with homology to existing sequences in the chromosome; in contrast to most other transposons Mu does not need a separate vector system since it is itself a vector A wide variety of useful mutants of Mu have been ...
Gene Section DNMT3B (DNA (cytosine-5-)-methyltransferase 3 beta) Atlas of Genetics and Cytogenetics
... Figure 2. A: The general structure of DNMT3B protein. The N-terminal domain contains a proliferating cell nuclear antigen-binding domain, a nuclear localization signal, a tetrapeptide PWWP, essential for DNMT binding to chromatin, an ATRX cysteine-rich zinc finger DNA-binding motif and a polybromo h ...
... Figure 2. A: The general structure of DNMT3B protein. The N-terminal domain contains a proliferating cell nuclear antigen-binding domain, a nuclear localization signal, a tetrapeptide PWWP, essential for DNMT binding to chromatin, an ATRX cysteine-rich zinc finger DNA-binding motif and a polybromo h ...
CPS 302 Society, Science, and Technology
... down sugar. There are tens of thousands of proteins in the body and they all have different forms and functions. They build and control life at the chemical level. An understanding of the ribosome's innermost workings is important for a scientific understanding of life. This knowledge can be put to ...
... down sugar. There are tens of thousands of proteins in the body and they all have different forms and functions. They build and control life at the chemical level. An understanding of the ribosome's innermost workings is important for a scientific understanding of life. This knowledge can be put to ...
Poster PDF - Urban Barcode Project
... of these products with claims of a plethora of probiotic bacteria in these yogurts. Yogurt F Probiotics have been used to both advertise and treat a wide range of diseases and ailments that affect humans and animals, including digestive, gastrointestinal, and immune system problems. The most common ...
... of these products with claims of a plethora of probiotic bacteria in these yogurts. Yogurt F Probiotics have been used to both advertise and treat a wide range of diseases and ailments that affect humans and animals, including digestive, gastrointestinal, and immune system problems. The most common ...
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses
... expression of different genes by cells with the same genome. 6. Describe at what level gene expression is generally controlled. Fig. 19.3 Other examples are places where expression can be turned on or off, accelerated, or slowed down. Activity: “Control of Transcription”, “PostTranscriptional Contro ...
... expression of different genes by cells with the same genome. 6. Describe at what level gene expression is generally controlled. Fig. 19.3 Other examples are places where expression can be turned on or off, accelerated, or slowed down. Activity: “Control of Transcription”, “PostTranscriptional Contro ...
File
... • After two DNA strands separate, under proper conditions the strands can come back together • Process is called annealing or renaturation • Three most important factors: – Temperature – best at about 25C below Tm – DNA Concentration – within limits higher concentration better likelihood that 2 com ...
... • After two DNA strands separate, under proper conditions the strands can come back together • Process is called annealing or renaturation • Three most important factors: – Temperature – best at about 25C below Tm – DNA Concentration – within limits higher concentration better likelihood that 2 com ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.