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Out-of-Africa Theory: The Origin Of Modern Humans
Out-of-Africa Theory: The Origin Of Modern Humans

...  Mitochondria are structures within cells that convert the energy from food into a form that cells can use. Although most DNA is packaged in chromosomes within the nucleus, mitochondria also have a small amount of their own DNA. This genetic material is known as mitochondrial DNA or mtDNA. In human ...
RNA Structure and Function
RNA Structure and Function

... 1. The RNA represents a copy of a small segment of the information contained within the DNA. It is convenient to view the DNA as a giant “recipe book” that contains all of the information necessary to “make” the organism in which it is found. The “recipes” each specify the ingredients and their orde ...
Document
Document

... How much data storage does 1 human genome require? About 1.5 GB (2 CDs) if your stored only one copy of each letter. For the raw format containing image files and base quality data 2-30 ...
DNA, RNA, and Protein Synthesis Note Packet
DNA, RNA, and Protein Synthesis Note Packet

... 3. The DNA inherited by an organism dictates the synthesis of certain proteins. Proteins are the link between ___________________________. The proteins that are made will determine what _____________ show up in the offspring. 4. _______________________: The process by which DNA directs the synthesi ...
Basic Bioinformatics
Basic Bioinformatics

... – the lengths of the query and subject sequences should be within 20% of each other – There should be at least 30% identical amino acids – In this case we can be quite sure we have a good match BLAST also returns a fourth value, the bit score, which we are going to ignore. ...
Biology Mitosis / Meiosis 2012 – 2013 #3
Biology Mitosis / Meiosis 2012 – 2013 #3

... A. Deletion = part of the chromosome is broken off and lost B. Duplication = part of the chromosome breaks off and attaches to its homologous chromosome C. Inversion = part of a chromosome breaks off and reattaches backwards D. Translocation = part of a chromosome breaks off and attaches to a Non-ho ...
BDOL Interactive Chalkboard
BDOL Interactive Chalkboard

... From DNA to Protein • Genes are small sections of DNA. Most sequences of three bases in the DNA of a gene code for a single amino acid in a protein. • Messenger RNA is made in a process called transcription. The order of nucleotides in DNA determines the order of nucleotides in ...
Chapter 12
Chapter 12

... – DNA unwinds at the origin to produce a “bubble,” – replication proceeds in both directions from the origin, and ...
Basic Bioinformatics - NIU Department of Biological Sciences
Basic Bioinformatics - NIU Department of Biological Sciences

... – the lengths of the query and subject sequences should be within 20% of each other – There should be at least 30% identical amino acids – In this case we can be quite sure we have a good match BLAST also returns a fourth value, the bit score, which we are going to ignore. ...
Basic Bioinformatics
Basic Bioinformatics

... – the lengths of the query and subject sequences should be within 20% of each other – There should be at least 30% identical amino acids – In this case we can be quite sure we have a good match BLAST also returns a fourth value, the bit score, which we are going to ignore. ...
Mechanism, and Role in Recombination Type-1
Mechanism, and Role in Recombination Type-1

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20_Lecture_Presentation_PC

... Problems Associated with Animal Cloning • In most nuclear transplantation studies, only a small percentage of cloned embryos have developed normally to birth, and many cloned animals exhibit defects • Many epigenetic changes, such as acetylation of histones or methylation of DNA, must be reversed i ...
Dangerous Ideas and Forbidden Knowledge, Spring 2005 Lab 2
Dangerous Ideas and Forbidden Knowledge, Spring 2005 Lab 2

... drop of blood, a single hair follicle, or a cheek cell and make enough to study. Prior to PCR, this would have been impossible! This dramatic amplification is possible because of the structure of DNA, and the way in which cells naturally copy their own DNA. DNA in our cells exists as a double-strand ...
Demo notes update - UK Association for Science and Discovery
Demo notes update - UK Association for Science and Discovery

... Explain that everyone will have a chance to practice gel loading before loading their samples and that you will come around and demonstrate before they get started. While they are waiting for their demonstration they should use their worksheet to plan where they are going to load their three samples ...
Normal pairing
Normal pairing

... The UV photoproducts significantly perturb the local structure of the double helix. These lesions interfere with normal base pairing. The C to T transition is the most frequent mutation , but UV light also induces other base substitutions (transversions) and frameshifts, as well as larger duplicatio ...
Presentation453.12
Presentation453.12

... radioactivity by using ddNTP analogues that carry a fluorescent group, a different chemical group fluorescing at a different color for each of the 4 bases, so that a single reaction and single lane (instead of 4) can be used to completely sequence a DNA molecule ...
Chapter 18
Chapter 18

... 18.4 What Other Tools Are Used to Study DNA Function? 18.5 What Is Biotechnology? 18.6 How Is Biotechnology Changing Medicine and Agriculture? ...
Presentation 1 Guidelines
Presentation 1 Guidelines

... compared to the one in part B. GC base pairs have three hydrogen bonds compared with AT base pairs, which only have two. C18. Its nucleotide base sequence. C19. Complementarity is important in several ways. First, it is needed to copy genetic information. This occurs during replication, when new DNA ...
Molecular Genetics S Brown 30th May 2014
Molecular Genetics S Brown 30th May 2014

... • Researchers from the UK are taking part in a global study of the link between genetic variation and diseases. • Scientists from the UK, US and China will work together to create the largest DNA database in the world. • The 1000 Genomes Project will map the DNA make-up of 1000 people from different ...
Integrated Programme Sec 2 SBGE, LSS Biology Module Topic
Integrated Programme Sec 2 SBGE, LSS Biology Module Topic

... Another gene that confers antibiotic resistance can also be inserted Culture the bacteria on agar plates containing the antibiotic  Bacteria with the modified plasmid will survive and grow Colonies of recombinant bacteria can be identified Pure cultures of the recombinant bacteria can be cultivated ...
Mutation
Mutation

... copied exactly during DNA replication. Rarely, however, errors occur, giving rise to novel sequences. These errors are called replication-dependent mutations. ...
ASE FS21 GM handout (DOC 756Kb)
ASE FS21 GM handout (DOC 756Kb)

... Click on the chromosome column, you will be able to zoom in (and out) until you can clearly see individual genes, Surf around the genome for a few minutes and get a feel for the genome Can you identify Gene structure, specifically Introns and Exons A gene sequence with introns and exons is the genom ...
Class4_Synthetic_Genetics
Class4_Synthetic_Genetics

... -30 different mutants X TET-promoter collection -found many interactions between dissimilar genes -claimed that there are five times as many “negative” genetic interactions for essential genes when compared to non-essential genes -however, the cause of this may be due to the fact that the TET strain ...
Genetics and Genomics in Medicine Chapter 6 Questions Multiple
Genetics and Genomics in Medicine Chapter 6 Questions Multiple

... a) A microRNA normally works by binding to perfectly complementary sequences within an RNA transcript, usually an mRNA. b) Like the great majority of mRNAs an miRNA is usually produced as a larger precursor RNA that is capped and has a 3’ poly(A) tail. c) The precursor miRNA undergoes different type ...
Genomes 3/e
Genomes 3/e

... They can be multiallelic i.e. each SSLP can have a number of different length variant. They are Minisatellite or variable number of tandem repeats (VNTRs) • Repeat unit is up to 25bp in length (not evenly distributed found at ends) ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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