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Bio 101 General Biology 1
Bio 101 General Biology 1

... Departmental policy dictates that instructors do not allow students to keep tests. ...
MBG305_LS_01
MBG305_LS_01

... body (an exception is, for example, red blood cells which have no nucleus and therefore no DNA) – a total of ~1022 nucleotides! • Many DNA regions code for proteins, and are called genes (1 gene codes for 1 protein as a base rule, but the reality is a lot more complicated) – Name examples ...
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Ch. 7 (part 2)

... Nail-Patella Syndrome – Autosomal dominant: abnormal fingernails and absent (underdeveloped kneecaps) ...
Barbara McClintock
Barbara McClintock

... encouraged — at least until adolescence. Then Barbara’s mother became concerned that her children fit into adult society. By this time World War I had begun and Thomas was serving overseas as a military surgeon. Career guidance for their children was left to Sara. Barbara’s older sisters followed th ...
homologous structures
homologous structures

... composed of proteins B)A chromosome contains many genes which are composed of DNA C)A gene is composed of proteins which are found on the chromosomes D)A chromosome contains many genes which are composed of proteins ...
Protein Synthesis – Level 1
Protein Synthesis – Level 1

... 3. Prior to leaving the nucleus, what will be added to the mature mRNA? What will the mRNA look like after this occurs? What is the purpose of this processing? The 5’ end will get a “cap” and the 3’ end will get a poly-A tail (AAAAAAA). These will help prevent the mRNA from degrading too quickly in ...
Engineering Life: Building a Fab for Biology
Engineering Life: Building a Fab for Biology

... see which combinations worked most effectively. ManufacWhat we mean by that term is well illustrated by Elowitz turing just two variants of each gene would mean synthesizing and Leibler’s ring oscillator, which they began as an attempt to 29, or 512, constructs, for a total of about six million nucl ...
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Transpeptidase, or PBP

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... Additional experiments reveal that the dre1 and dre2 mutants are not defective in postreplication mismatch repair. 2C (6 points). What repair protein is most likely to be defective in the dre1 mutant? Briefly explain your answer. The most likely possibility is that the dre1 mutant is uracil glycosyl ...
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... enzyme adenosine deaminase (ADA) In this protocol, a cloned complementary DNA (cDNA) for ADA was introduced into lymphocytes that had been removed from each patient After culturing, each subject received transfusion with her own genetically engineered lymphocytes, which synthesized ADA, at regular i ...
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... swallowed it. – mitochondria make their own ribosomes, which are of the prokaryotic 70S type, not the eukaryotic 80S type. – mitochondria are sensitive to many bacterial inhibitors that don’t affect the rest of the eukaryotic cell, such as streptomycin, chloramphenicol, rifampicin. – mitochondrial p ...
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GENETIC BASICS OF VARIATIONS IN BACTERIA

... D. Genetic changes occurring within a single cell. Point mutations, single nucleotide changes in the DNA, can have a number of consequences. In coding regions they may alter an amino acid in a polypeptide. The effect may be deleterious (inactivation or lower activity) or beneficial (enhanced or new ...
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Sample newsletter January 2017

... In schools, safer, cheaper dye solutions are used to stain the entire gel, including the DNA, after electrophoresis. Suitable stains include Azure A and Azure B, Toluidine blue O and Nile blue sulphate. This type of stain is not thought to intercalate within the DNA double helix, but instead binds i ...
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pGLO Bacterial Transformation- Pre-Lab

... 2. Scientists often want to know if the genetically transformed organism can pass its new traits on to its offspring and future generations. To get this information, which would be a better candidate for your investigation, an organism in which each new generation develops and reproduces quickly, or ...
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crispr - UNM Biology

... byproduct. However, The spacer between each repeat varied between organisms. • Jansen et al, 2002 Four Cas genes near the repeats with helicase, and nuclease protein domains. • Together coined the term CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats describing the loci ...
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... The four types of histone are homologous and similar in structure An histone octomer consists of a (H3)2(H4)2 tetramer and a pair of H2A-H2B dimers to form a left-handed superhelical ramp around which the DNA wraps. Each histone has an flexible N-terminal tail rich in Lys and Arg that extends out fr ...
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... suggested that genes coded for enzymes  each disease (phenotype) is caused by non-functional gene product ...
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pGLO Bacterial Transformation- Pre-Lab

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Comparative Study of DNA Isolated from Increasing

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DNA databases Rape in the US Victims States with Offender Laws

... In the early 90s, the DNA community realized that DNA types could be used to do more than just “confirm” an individual was present or involved in a criminal act. DNA types (RFLP profiles), stored in a database, could be used to proactively link and solve crimes just like fingerprints were being used ...
pGLO Transformation Lab - Tamalpais Union High School District
pGLO Transformation Lab - Tamalpais Union High School District

... resistance can be genetically transformed into plants. In bio-remediation, bacteria can be genetically transformed with genes enabling them to digest oil spills. In medicine, diseases caused by defective genes are beginning to be treated by gene therapy; that is, by genetically transforming a sick p ...
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Botany LBC-101 Unit-II lect

... cytoplasm. They consist of ribosomal RNA (rRNA). Their DNA contains a single, circular molecule, which is compact and tightly wound. No protein is associated with DNA. 7. The archaebacterial cell may contain plasmids, which are small, circular pieces of DNA. They can duplicate independent of a large ...
pGLO Transformation Lab - Tamalpais Union High School District
pGLO Transformation Lab - Tamalpais Union High School District

... resistance can be genetically transformed into plants. In bio-remediation, bacteria can be genetically transformed with genes enabling them to digest oil spills. In medicine, diseases caused by defective genes are beginning to be treated by gene therapy; that is, by genetically transforming a sick p ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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