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Slide 2
Slide 2

... organism has 2 pairs of each chromosome. However, to pass on the information to the next generation, the information has to be “halved”, as the other half has to be provided by the other parent. This process of reduction of the genetic information during the formation of the gametes is called meiosi ...
Transcription of a genome
Transcription of a genome

... The initial product of gene expression is always Ribo Nucleic Acid (RNA) RNA is a single stranded nucleic acid species The pentose sugar in RNA is Ribose Thymine is not a base used in RNA. It is replaced by Uracil. Cells contain three major species of RNA: transfer RNA (tRNA), ribosomal RNA (rRNA) a ...
Transcription
Transcription

... Recognized by small nuclear ribonucleoprotein particles (snRNPs) that catalyze the cutting and splicing reactions. Internal intron sequences are highly variable even between closely related homologous genes. Alternative splicing allows different proteins from a single ...
L-1 - West Ada
L-1 - West Ada

... Is the division of a diploid cell to make 4 New ________ cells. (Diploid, Gamete or ...
Chromosomes, DNA, and Genes
Chromosomes, DNA, and Genes

... • Jack jumper ant, with only 2 chromosomes! (1 pair) ...
Unit V DNA RNA Protein Synthesis
Unit V DNA RNA Protein Synthesis

... of prokaryotic cells; contains genes that code for traits. Each species has its own number of chromosomes. 2. Deoxyribonucleic Acid (DNA) A biological macromolecule that codes for the genetic information for traits (carried in proteins) in living organisms. It can make copies (replication) of itself ...
Transcription and the Central Dogma
Transcription and the Central Dogma

... from many genes averages out to this. – The closer these 2 regions actually are to the consensus sequences, the “stronger” the promoter, meaning the more likely RNA polymerase binding and transcription will occur. ...
Section 7.1 DNA Cloning with Plasmid Vectors
Section 7.1 DNA Cloning with Plasmid Vectors

... numbers of recombinant DNA molecules that include the fragment of DNA originally linked to the vector. Two types of vectors are most commonly used: E. coli plasmid vectors and bacteriophage λ vectors. Plasmid vectors replicate along with their host cells, while λ vectors replicate as lytic viruses, ...
Guidance on the significance of chemical
Guidance on the significance of chemical

... identification of genetic variability since the previous COM guidance through the work on the human genome project and the availability of affordable whole genome scanning 12. The identification of genetic variations, such as single nucleotide polymorphisms (SNPs), where a DNA sequence exists in two ...
Experimental Ecology
Experimental Ecology

... • Advantages: Can be used to detect viable but non-culturable microorganisms; can be used to count microbes; can be automated; can be used in situ in samples • Problems: cross-reactivity, can’t raise antibodies if you don’t have a pure culture and so can’t predict if any other microbe will also rea ...
Chapter 6 From DNA to Protein: How Cell Read the Genome
Chapter 6 From DNA to Protein: How Cell Read the Genome

... RNA splicing might occurred before or after polyadenylation ...
C - mhs
C - mhs

...  Result is the so-called genetically modified organisms (GMOs) • Invaluable tool in modern biotechnology today • Commercial products that are currently much used • Green fluorescent protein (GFP) can be used as a marker – A jellyfish gene codes for GFP – The jellyfish gene is isolated and then tran ...
What is a gene mutation and how do mutations occur Article
What is a gene mutation and how do mutations occur Article

... A gene mutation is a permanent change in the DNA sequence that makes up a gene. Mutations range in size from a single DNA building block (DNA base) to a large segment of a chromosome. Gene mutations occur in two ways: they can be inherited from a parent or acquired during a person’s lifetime. Mutati ...
Unit Plan – DNA, RNA, and Protein Synthesis Honors Biology Ninth
Unit Plan – DNA, RNA, and Protein Synthesis Honors Biology Ninth

... The cell is an amazingly fine-tuned machine, working nonstop to maintain homeostasis of organisms. The most crucial of all maintenance systems of the cell’s processes is protein synthesis. Without proteins, life would not exist as we know it. This unit provides that understanding of the basic cellul ...
9 . What is a gene mutation and how do mutations occur
9 . What is a gene mutation and how do mutations occur

... A gene mutation is a permanent change in the DNA sequence that makes up a gene. Mutations range in size from a single DNA building block (DNA base) to a large segment of a chromosome. Gene mutations occur in two ways: they can be inherited from a parent or acquired during a person’s lifetime. Mutati ...
08MicrobialGenetExamIIAnswers
08MicrobialGenetExamIIAnswers

... origins of replication fire, one plasmid is likely to be replicated more frequently than the other. This may be because its origin has slightly higher affinity for the initiation proteins, it is smaller and therefore replicates faster, or just by chance, over time, each cell will not inherit equal c ...
Misconceptions, misunderstandings and questions students
Misconceptions, misunderstandings and questions students

... In my experience and in discussions with colleagues, there is a consensus that students do not have an understanding of how traits are inherited and expressed. The topics of meiosis and protein synthesis are extremely challenging to teach for a variety of reasons. They are often taught in isolation, ...
Gene_expression
Gene_expression

... complete ladder of bands would be seen on the gel (not shown). (B) An actual footprint used to determine the binding site for a human protein that stimulates the transcription of specific eucaryotic genes. These results locate the binding site about 60 nucleotides upstream from the start site for RN ...
I. DNA, Chromosomes, Chromatin, and Genes II. DNA
I. DNA, Chromosomes, Chromatin, and Genes II. DNA

... 1. __________________________ (enzyme) attaches at a specific location on DNA 2. The enzyme then causes the DNA strands to separate from one another and allow one of the DNA strands to be ________________ 3. mRNA nucleotides are floating around in the nucleus find their complement on the DNA stand a ...
The Basic Process of Evolution
The Basic Process of Evolution

... The DNA strand in E. coli contains about 4 million base pairs, and these base pairs are organized into about 1,000 genes. A gene is simply a template for a protein, and often these proteins are enzymes. An enzyme is a protein that speeds up a particular chemical reaction. For example, one of the 1,0 ...
Genes As Information
Genes As Information

... You have two pairs for each chromosome ...
12_Lecture_Presentation
12_Lecture_Presentation

... 12.9 Genetically modified organisms raise concerns about human and environmental health  Scientists use safety measures to guard against production and release of new pathogens  Concerns related to GM organisms – Can introduce allergens into the food supply – FDA requires evidence of safety befo ...
Exercise 5
Exercise 5

... cellular proteins are translated. At least some of this maternal RNA cannot be translated by polysomes as a message for proteins: translational stop signals have been found in all frames in repeat and single-copy portions of maternal transcripts. In such molecules, the actual message may be interspe ...
Pre-AP Unit 4 Homework
Pre-AP Unit 4 Homework

... Before insulin was discovered, the only treatment for diabetes was a very strict diet that was low in calories and carbohydrates. This type of diet lengthened the lives of diabetic patients, but, without insulin, the patients still died within a few years. In 1921, two Canadian researchers discovere ...
Chapter 17.
Chapter 17.

... Inheritance of metabolic diseases suggested that genes coded for enzymes  each disease (phenotype) is caused by non-functional gene product ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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