• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Ivy Mead 24 February 2011 Bioinformatics Lab report 3 The analysis
Ivy Mead 24 February 2011 Bioinformatics Lab report 3 The analysis

protein synthesis overview
protein synthesis overview

... PROTEIN SYNTHESIS OVERVIEW • RNA LINKS DNA’S GENETIC INSTRUCTIONS FOR MAKING PROTINS TO THE PROCESS OF PROTEIN SYNTHESIS • RNA COPIES (TRANSCRIBES) THE MESSAGE FROM DNA AND THEN TRANSLATES THAT MESSAGE INTO A PROTEIN • THE LINEAR SEQUENCE OF NUCLEOTIDES IN DNA DETERMINES THE LINEAR SEQUENCE OF AMIN ...
blueprint of life
blueprint of life

... evolutionary relationships is DNA hybridization. DNA hybridization is used to identify the similarities between two different organisms by comparing their DNA. The process of DNA hybridization is outlined below.  2 strands of DNA are collected. One from one species the other from another species.  ...
LiMA overview
LiMA overview

... • LiMA is generic – all bacteria tested contain NAD-dependent DNA ligase. It is difficult to ensure that direct PCR is generic. • LiMA is more sensitive than direct PCR. LiMA involves lysis of the bacilli and release of many ligase molecules which amplify the target prior to sampling for PCR. This i ...
Educational Items Section Telomeres Atlas of Genetics and Cytogenetics in Oncology and Haematology
Educational Items Section Telomeres Atlas of Genetics and Cytogenetics in Oncology and Haematology

... I.3.7. WRN (Werner syndrome gene product): - RecQ subfamily of helicases. - Required for DNA replication. - Involved in control of genomic stability. N.B. Therefore, telomere function can be compromised by affecting telomere-binding protein function(s). ...
A Novel Method to Detect Identities in tRNA Genes Using Sequence
A Novel Method to Detect Identities in tRNA Genes Using Sequence

... We applied the method to Class I tRNAs to detect characteristic sites. We found that about 40% of characteristic sites that we detected are identities that have been detected experimentally, and that the remaining characteristic sites are in T and D domains which are the elbow regions of tRNAs. This ...
Functional Photonics for Single Bioentities a biophotonics Platform
Functional Photonics for Single Bioentities a biophotonics Platform

... Metallic nanoparticles (NP) (from 100nm to 102 nm) can be used for nonfluorescent labels. Interaction with probe light is through light scattering, plasmon resonance or local enhancement of nonlinear optical response. We will functionalise a number of NP configurations (e.g. dots, shells, rods, ...) ...
Topic 10 (From Genotype to Phenotype)
Topic 10 (From Genotype to Phenotype)

... • As the single-stranded messenger RNA (mRNA) peels away from the gene – The DNA strands rejoin ...
Detecting multiple DNA human profile from a mosquito blood meal
Detecting multiple DNA human profile from a mosquito blood meal

... The quantification analysis showed that 0.019 ng/µL human DNA was obtained from one individual; 0.010 ng/µL male DNA when three individuals were used, and 0.256 ng/µL human DNA (0.007 ng/µL of male DNA) when four individuals were used. The value of statistical analysis of likelihood ratio (LR) was d ...
PDF - The Journal of General Physiology
PDF - The Journal of General Physiology

... three different dates corresponding to 0, 40, and 50 per cent of the incorporated p3~ decayed. A total of nine different emulsions was counted; the average star size extended from 7.4 to 15.4 rays per star. The average of these determinations for the average number of p~2 atoms per phage particle, N ...
2: Enzymes
2: Enzymes

... the outside of the protein, whilst non-polar, hydrophobic groups tend to lie on the inside, insulated from water. ...
ConjugationSlides
ConjugationSlides

... Bacterial Conjugation • Conjugation will be used as a means to transfer genetic information from one bacteria to another • Cells with a F+ plasmid or a R plasmid can transfer their DNA to other bacteria • On the F and R plasmids, DNA transfer begins at a specific sequence, OriT, the Origin of Transf ...
Linkage, Crossing Over, and Chromosome Mapping
Linkage, Crossing Over, and Chromosome Mapping

... 2-strand DCO yields 0 recombinants 3-strand DCO yields 2 recombinants 4-strand DCO yields 4 recombinants ...
Common DNA sequences with potential for detection of genetically
Common DNA sequences with potential for detection of genetically

... These borders are present on all T-DNA vectors and enable random transfer of foreign DNA to the plant genome. A promoter and a terminator are required to express genes in plant cells. A large number of promoters can be used including the TR1? promoter, the ocs promoter, and the nos promoter from Agr ...
Microbial Genetics (Kroening)
Microbial Genetics (Kroening)

... that microorganisms have genes, the structure of DNA, and the role of DNA in heredity (1940’s and 50’s), microbes have been used extensively to explore the structure, function, regulation, and evolution of genes. In addition, microbial genetics is essential for molecular biological studies and manip ...
Integration of experimental evidence
Integration of experimental evidence

... machines at figuring out what is true, and the combination of humans and machines is virtually unbeatable. Our challenge is to figure out ways to combine those two forces to propagate evidencebased annotation and to make it easy for researchers to distinguish assertions that have experimental eviden ...
agrico.rakesh_linkage
agrico.rakesh_linkage

... Two-point crosses are good but not adequate; they have disadvantages. • Underestimate map distance when it is > 10 cM (double crossovers cancel each other). • Provide no information about relative positions of two linked genes. • Do not allow detection of double crossovers. Cure: Three-point cross ...
Chapter 11 from book
Chapter 11 from book

... RNA polymerase and direct it to specific promoters Global gene regulation: Genes that encode proteins with related functions may have a different location but have the same promoter sequence—they are turned on at the same time. Sporulation occurs when nutrients are depleted—genes are expressed seque ...
The Ancestry of a Gene - 2009
The Ancestry of a Gene - 2009

... does not become fixed in the population, rather crossing over during the fixation process entails that at every locus the genes have an ancestral pool rather than a common ancestor. If one wants to think of mutations becoming fixed, mutations must be viewed as the base pair which mutates, not the ge ...
human lymphoblastoid cells. and unbalanced translocation in p53
human lymphoblastoid cells. and unbalanced translocation in p53

... times that of the wild-type allele, which corresponds to about two copies of the tk gene, the LOH mutant was classified as homozygous (Table 1). We previously reported the mutational spectra of TK mutants from TK6 and WTK-1 cells (17). Almost all TK mutants spontaneously arising and induced by X-ray ...
AQA(B) AS Module 2 - heckgrammar.co.uk
AQA(B) AS Module 2 - heckgrammar.co.uk

... Structure. DNA is a stable polynucleotide. The double-helix structure of the DNA molecule in terms of: the components of DNA nucleotides; the sugarphosphate backbone; specific base pairing and hydrogen bonding between polynucleotide strands (only simple diagrams of DNA structure are needed; structur ...
Unit 10.1.4 - Measuring Genetic Variation using Molecular Markers
Unit 10.1.4 - Measuring Genetic Variation using Molecular Markers

... Allozymes are controlled by codominant alleles which means that it is possible to distinguish between homozygotes and heterozygotes. For monomeric enzymes (i.e. consisting of a single polypeptide), plants homozygous for that locus will produce one band whereas heterozygous individuals will produce t ...
Gene sequences useful for predicting relatedness of whole
Gene sequences useful for predicting relatedness of whole

... Prediction intervals were calculated and the upper/lower limits of these intervals were used to determine the cut-point for the desired 70 % alignment. ...
Recurring Themes in the Study of Biology
Recurring Themes in the Study of Biology

... remain ordered and complex so life requires energy.  Energy can be stored in different forms, for example, light, chemical, kinetic, or thermal  The energy exchange between an organism and its environment often involves energy transformations  Energy flows through an ecosystem, usually entering a ...
ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... Typically, unmethylated clusters of CpG pairs are located in tissue-specific genes and in essential housekeeping genes. (House-keeping genes are involved in routine maintenance roles and are expressed in most tissues.) ...
< 1 ... 145 146 147 148 149 150 151 152 153 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report