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Journal of Advances In Science and Technology
Journal of Advances In Science and Technology

... has dissolved, and specialized protein fibers called microtubules have formed a spindle apparatus, as also occurs in the metaphase of mitosis. These microtubules then attach to the kinetochore protein disks on the two centromeres of the homologous pair of chromosomes. However, there is an important ...
Motif Finding Problem
Motif Finding Problem

... • Located within the RR are the Transcription Factor Binding Sites (TFBS), also known as motifs, specific for a given transcription factor • TFs influence gene expression by binding to a specific location in the respective gene’s regulatory region - TFBS ...
Your EasyGuide to DNA Polymerases
Your EasyGuide to DNA Polymerases

BlastLecture8
BlastLecture8

... (translated in all six reading frames) – essential for searching EST databases. and in the interests of completeness there is: • tblastx: searches a DNA sequence (translated in all six reading frames) against a DNA database (translated in all six reading frames). finally • Psi-blast an iterative pro ...
Semester 2 Final Exam Study Extravaganza!
Semester 2 Final Exam Study Extravaganza!

... 6. Compared to small cells, large cells have more trouble… a) Dividing. b) Producing daughter cells. ...
PART II
PART II

... First of all it is important to know the source of the gene donor and whether the source is associated with any known toxic or allergenic history which might conceivably be transferred during the transformation process. If such products exist in the donor, analysis should be conducted to assure that ...
Genotyping of Her1 SNP`s in familial breast cancer by restriction
Genotyping of Her1 SNP`s in familial breast cancer by restriction

... Breast cancer is one of the most common malignancies affecting women worldwide. In India, breast cancer is the second most common malignant condition among women. Genetic predisposition for familial early onset of breast cancer accounts for approximately 5-10% of all breast cancers. Mutations in two ...
22 Fungal Genetics Newsletter bimD
22 Fungal Genetics Newsletter bimD

... Such bimD function differs from that of members in the four Uvs epistatic groups of A. nidulans which increasingly is becoming better defined [superceding even recent reviews; e. g., Kafer and May 1998 In Nickoloff and Hoekstra (eds), DNA Damage and Repair, vol.1, Humana, Totowa NJ, p. 477-502]. Cur ...
DNA Replication Replication begins simultaneously on several
DNA Replication Replication begins simultaneously on several

... Replication begins simultaneously on several chromatin threads & continues until all DNA has ...
The geranylgeranyl pyrophosphate synthase gene from Ginkgo
The geranylgeranyl pyrophosphate synthase gene from Ginkgo

LAB 1: Scientific Method/Tools of Scientific Inquiry
LAB 1: Scientific Method/Tools of Scientific Inquiry

... to load, being sure the tip is in the liquid submerging the gel but NOT deep into the well (you don’t want to poke a hole in the gel!) 5. use a finger from your off hand to steady the micropipettor as shown in the picture and slowly depress the plunger to the first stop to expel the liquid into the ...
IN THE UNITED STATES DISTRICT COURT FOR THE
IN THE UNITED STATES DISTRICT COURT FOR THE

... polymorphisms linked to coding region alleles using amplified DNA with a primer pair spanning a non-coding DNA region during the term of the '179 Patent in the provision of its HLA typing services. The provision of these testing services in this manner utilizes the invention as claimed in the '179 P ...
6.1 Mutation
6.1 Mutation

... Your DNA has instructions to make the proteins!!!! Changes in your DNA are copied into your RNA. That faulty RNA is used to pick the amino acids in your protein. What could possibly happen if you have the wrong amino acids???? ...
Slide 1
Slide 1

... 6.The inactivation of repeated genes: The repeated genes in eukaryotic genome can both increase the gene information and protect the gene damage from the gene disappeared. In another hand, the repeated genes can delay the early death of the important cell. If one copy of repeated gene is damaged and ...
Saccharomyces exiguus
Saccharomyces exiguus

... Sourdough bread http://www.recipetips.com/kitchen-tips/t--1125/breads-using-starters.asp DNA – http://www.dnamnd.med.usyd.edu.au/ Screenshot http://www.gen.tcd.ie/cgi-bin/khwolfe/gene.pl?name=SSB1&junk=no Magnifying glass http://blog.labnotes.org/2006/05/31/pr-tools-and-bag-of-tricks/ ...
Novel DNA Polymerase Increases Efficiency of Multiple PCR
Novel DNA Polymerase Increases Efficiency of Multiple PCR

... to SD polymerase in PCR, including amplification of GC-rich and complex secondary structures, ...
Mouse Direct PCR Kit
Mouse Direct PCR Kit

Is this an inducible or repressible operon?
Is this an inducible or repressible operon?

... Deletions and duplications can result from unequal crossing over. The consequences of chromosomal mutation varies widely and are NOT missense, silent, frameshift, nonsense. ...
Bioanalytical chemistry 8. Gel electrophoresis and blotting
Bioanalytical chemistry 8. Gel electrophoresis and blotting

... prepared by PCR and so it would be double stranded. The same could be true for all techniques, including southern, FISH and SKY. Q: Is Eastern blotting or lectin blotting an important part of identifying post translational mods in lipid and carbohydrates or proteins or is it better to just use a com ...
Writing Information into DNA
Writing Information into DNA

Transcription factors Oct-1 and NF-YA regulate the p53
Transcription factors Oct-1 and NF-YA regulate the p53

... Mapping of the DNA damage responsive elements in the GADD45 promoter Our previous reports have demonstrated that the GADD45 promoter is strongly responsive to multiple genotoxic stress, such as MMS and UV radiation. The activation of the GADD45 promoter by DNA damage is independent of normal cellula ...
Dörte Harpke - Lehrstuhl Technische Informatik der MLU Halle
Dörte Harpke - Lehrstuhl Technische Informatik der MLU Halle

... family the ITS region is subjected to concerted evolution, which means the homogenisation among the copies through processes like gene conversion and unequal crossing over. An incomplete concerted or non-concerted evolution caused by e.g. hybridisation, disadvantageous loci or polyploidy results in ...
Z. Naturforsch. 66c
Z. Naturforsch. 66c

... The basis for resistance to glyphosate in RR soybeans is the insertion of a transgene that originates from the Agrobacterium strain CP4 (CP4EPSPS) and specifies a glyphosate-insensitive EPSPS variant (Padgette et al., 1995). Molecular characterization studies determined that the genetic insertion in ...
Module IV Nucleus
Module IV Nucleus

Lab 1 genomic DNA
Lab 1 genomic DNA

... Phenol is a strong denaturing agent for proteins. In phenol extractions, proteins partition into the organic phase (and interface) whereas nucleic acids partition in the aqueous phase. Usually phenol is used in a 1: 1 mixture with chloroform since deproteinization is more effective when two differen ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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