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1. Explain what is meant by each of the following terms. Gene
1. Explain what is meant by each of the following terms. Gene

... Suggest one advantage to the parents of knowing whether the unborn child will or will not suffer from cystic fibrosis. ...
A novel environment-sensitive biodegradable polydisulfide with
A novel environment-sensitive biodegradable polydisulfide with

... and polymers [13−16], are non-immunogenic, easy to use and can be readily produced in a large scale at low cost. However, the main drawbacks for catioinic delivery systems are their cytotoxicity [17] and the low delivery efficiency of nucleic acids. Innovative design of novel gene carriers is needed ...
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BIOMONITORING OF GeNOTOxIcITy OF SHALLOW WATeRS

... significant only in the biomonitoring IV, comparative to PPL and YP control sites. Biomonitoring IV was carried out at the end of a period when the EACF research station operated with many people, resulting in an increase of contaminants discharge that caused the effect shown on Figure 4. Difference ...
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Aipotu Part III: Molecular Biology

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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