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Warm up
Warm up

... Checkpoint question: Characteristics of Life (example: fish)(5 min) Video: Viruses and Bacteria…10 minutes -discuss differences between viruses and bacteria Cool down question : (5 min) Viruses / Work on assignment (7 min) -students write answer…due by end of the period Assignment: Describe at least ...
human genetic disorders part 2–diagnosis and treatment
human genetic disorders part 2–diagnosis and treatment

... is blood sample which is a very good source of DNA or RNA for molecular diagnostics. Alternative to blood samples are Mouthwashes or buccal scrapes that may be collected very noninvasively and these samples are useful in population screening studies. To obtain fetal DNA, chorionic villous biopsy or ...
Automated Targeted Locus Amplification (TLA) Technology for
Automated Targeted Locus Amplification (TLA) Technology for

... the basis of selection. DNA is cross-linked, fragmented and ligated. Only one to a few primer pairs specific for a genetic locus of interest are required for the amplification of an entire locus. Any gene of interest can be amplified by TLA using a primer pair specific for the gene of interest. Gene ...
Automated Targeted Locus Amplification for Targeted
Automated Targeted Locus Amplification for Targeted

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... you lose a gene, it starts to grow uncontrollable, another genetic mistake causes it to move further down the pathway towards cancer. This happens several times until it becomes a carcinoma and then with other mutations it begins to metastasize to other locations. ...
Practice exam 2 key
Practice exam 2 key

... (4 pts) [see figure] 2 pts for each chromosome. No centromeres indicated (-1 pt). No deduction if non-recombinant chromosomes shown and ABDEA indicated as bridge between. d) Will the recombinant chromatids segregate normally during cell division? Explain. (5 pts) No (1 pt); decentric will be pulled ...
Beads on a string Bowater Biochem Soc Trans 2012
Beads on a string Bowater Biochem Soc Trans 2012

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Chapter 2. Structures of Nucleic Acids
Chapter 2. Structures of Nucleic Acids

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... • Long linear polymers of molecules called amino acids • Information for synthesis of proteins is contained in the nucleic acid • Proteins have a variety of functions – Catalysis of chemical reactions (enzymes) – Structural (histones, cytoskeletal proteins) – Regulatory (transcription factors, growt ...
Every Biological Molecules Question
Every Biological Molecules Question

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Lab 17. Chromosomes and Karyotypes: How Do Two Physically

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Hybrid Plasmids Containing the Pyruvate
Hybrid Plasmids Containing the Pyruvate

... division = 1 kb) and the coordinates of specific targets relative to the HindIII target (H,) are listed in the text. The abbreviations for restriction sites are: A, AuaI; Ac, AccI; As, AspAI; B, BurnHI; Bg, BglII; C, CluI; H, HindIII; K, KpnI; P,PstI; Pv, PvuI; R, EcoRI; S , SmaI; Sa, Sun; Sp, SphI; ...
Unit H: Heredity and Reproduction
Unit H: Heredity and Reproduction

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Evolutionary population genomics

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DNA Duplications and Deletions Help Determine Health

... Each human’s genome is distinguished by extra, and sometimes missing, DNA that can powerfully impact everything from development to disease In 1991, both Science and Nature turned down James Lupski’s submission that described an unprecedented link between an inherited human disease and a novel chrom ...
method, a successful experiment must be verified by Southern blots
method, a successful experiment must be verified by Southern blots

... purified DNA has provided simple, rapid methods for the molecular cloning of mutant forms of genes ("eviction" of mutant genes) and for the introduction into yeast of mutant genes constructed in vitro ("transplacement" of mutant genes). This chapter will place these techniques in a conceptual framew ...
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Nucleic Acids Research

... an N-mer is added to the list described above, the positions in K or more sequences of the last N-mers to have differed from it at M or fewer positions are read from the new arrays and listed with it. Moreover, this set of positions is compared with all sets of positions already on the list, and if ...
Full Paper - Biotechniques.org
Full Paper - Biotechniques.org

... have been used to barcode algae, including 23S plastid rRNA (Sherwood and Presting 2007), 18S rRNA (Zimmerman et al. 2011), and rbcL (Paul et al. 2000). The rbcL gene is of particular interest because it codes for the large subunit of the protein ribulose-1,5-bisphosphate carboxylase oxygenase (RuB ...
Section D - Prokaryotic and Eukaryotic Chromosome Structure
Section D - Prokaryotic and Eukaryotic Chromosome Structure

... • Some genes contain terminator sequences requiring an accessory factor, the rho protein (ρ) to mediated transcription termination. • Rho binds to specific sites in the singlestranded RNA. • Rho protein (hexameric protein) binds to certain RNA structure (72bp) • Rho hydrolyses ATP and moves along th ...
Genetics - Stjosephcs.org
Genetics - Stjosephcs.org

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Introduction to Molecular Markers and their

Chapter 20 Regulation of Gene Expression in Eukaryotes
Chapter 20 Regulation of Gene Expression in Eukaryotes

... Activators are proteins that recognize specific short DNA sequences inducing the efficiency of the promoters. Co-activators are proteins required for a more efficient transcription. They do not bind DNA. Regulators of chromatin structure Figure 25.2 ...
At One Hundred: The Living Legacy of Francis Crick
At One Hundred: The Living Legacy of Francis Crick

... in the original). As Joshua Lederberg had already noted in 1956, information provided a new theory of specificity different from Pauling’s theory based on shape and size. The 1958 paper also proposed the existence of an adaptor molecule (i.e., tRNA) that mediated protein synthesis. Crick made many m ...
SAMPLE LITERATURE Please refer to included weblink for correct
SAMPLE LITERATURE Please refer to included weblink for correct

... Scientists document everything that happens during an experiment, including experimental conditions, thoughts and observations while conducting the experiment, and, of course, any data collected. Today, you'll be documenting your experiment in a laboratory notebook or on a separate worksheet. Before ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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