Staphylococcus haemolyticus lipase
... temperature range. In fact, the lipase L62 showed at 4³C as much as 30% of the activity shown by it at 28³C. In the range over 28³C, its inactivation energy was determined to be 316.4 kcal mol31 . The lipase L62 showed Ca2 -dependent stability at elevated temperatures. In the presence of Ca2 , it ...
... temperature range. In fact, the lipase L62 showed at 4³C as much as 30% of the activity shown by it at 28³C. In the range over 28³C, its inactivation energy was determined to be 316.4 kcal mol31 . The lipase L62 showed Ca2 -dependent stability at elevated temperatures. In the presence of Ca2 , it ...
Locked Nucleic Acid (LNA™)
... the oligonucleotide and great care must be taken to find the right design for your experimental purpose. While you will want to take advantage of the properties of LNA™ in order to achieve high target specificity it is important not to use too much LNA™ since this can result in a very “sticky” oligo ...
... the oligonucleotide and great care must be taken to find the right design for your experimental purpose. While you will want to take advantage of the properties of LNA™ in order to achieve high target specificity it is important not to use too much LNA™ since this can result in a very “sticky” oligo ...
ref. #28 of the TIBS article
... relative to the membrane but not in overall conformation. In the mutants with Arg or Lys residues in position 13, the charged residue is about halfway through the membrane and the charged sidechains might start to interact with the lipid head groups on the other side of the membrane. Effects of Asp ...
... relative to the membrane but not in overall conformation. In the mutants with Arg or Lys residues in position 13, the charged residue is about halfway through the membrane and the charged sidechains might start to interact with the lipid head groups on the other side of the membrane. Effects of Asp ...
Computational Biology - Bioinformatik
... have now yielded many thousands of miRNAs. This process continues with new miRNA appearing daily in various databases. miRNA sequences and annotations are compiled in the online repository miRBase (http://www.mirbase.org/). Each entry in the database represents a predicted hairpin portion of a miRNA ...
... have now yielded many thousands of miRNAs. This process continues with new miRNA appearing daily in various databases. miRNA sequences and annotations are compiled in the online repository miRBase (http://www.mirbase.org/). Each entry in the database represents a predicted hairpin portion of a miRNA ...
Identiflcation and typing of grapevine phytoplasma amplified by graft
... plants were determined by electron microscopy to be mycoplasma-like organisms (MLOs; CAUDWELL et al. 1971; MEIGNOZ et al. 1992). The name 'Phytoplasma' was later assigned to the group of plant MLOs which belong to the dass Mollicutes, characterized by their small genome size (680 to 1,600 kbp) and I ...
... plants were determined by electron microscopy to be mycoplasma-like organisms (MLOs; CAUDWELL et al. 1971; MEIGNOZ et al. 1992). The name 'Phytoplasma' was later assigned to the group of plant MLOs which belong to the dass Mollicutes, characterized by their small genome size (680 to 1,600 kbp) and I ...
PeptidePicker: a Tool for Determining Most Appropriate Peptides for
... triple quadrupole mass spectrometers. The first quadrupole is used to isolate a specific precursor peptide ion, the second quadrupole is used for collision-induced dissociation, and the third is used to isolate a characteristic fragment ion. In multiplexed analysis, specific precursor/product ion pa ...
... triple quadrupole mass spectrometers. The first quadrupole is used to isolate a specific precursor peptide ion, the second quadrupole is used for collision-induced dissociation, and the third is used to isolate a characteristic fragment ion. In multiplexed analysis, specific precursor/product ion pa ...
PDF - Oxford Academic - Oxford University Press
... SDPpred does not require information about the 3D protein structure, which instead can be used at the post-processing step to validate the predictions. There are also other approaches for comparative analysis of protein subfamilies. One of these methods, called DIVERGE (17) takes into account the di ...
... SDPpred does not require information about the 3D protein structure, which instead can be used at the post-processing step to validate the predictions. There are also other approaches for comparative analysis of protein subfamilies. One of these methods, called DIVERGE (17) takes into account the di ...
Comparative Analysis of ,Multiple Protein
... alignment of protein sequences: one attempts to align determined? Among highly conserved sequences(>50% homologous (ancestrally related) features,while the identity) it is very difficultto deduce which residues of other attempts to align functionally or spatially equiv- a pr0tei.nare necessary for f ...
... alignment of protein sequences: one attempts to align determined? Among highly conserved sequences(>50% homologous (ancestrally related) features,while the identity) it is very difficultto deduce which residues of other attempts to align functionally or spatially equiv- a pr0tei.nare necessary for f ...
The Thr to Met substitution of amino acid 118 in hepatitis B virus
... result, there is a high mutation rate of HBV reverse transcriptase due to the lacking of proofreading activity, which is the main cause of HBV mutation (Deng & Tang, 2011). HBV is a very compact virus with highly efficient usage of its genome. The small S gene of HBV is fully embedded in the polymer ...
... result, there is a high mutation rate of HBV reverse transcriptase due to the lacking of proofreading activity, which is the main cause of HBV mutation (Deng & Tang, 2011). HBV is a very compact virus with highly efficient usage of its genome. The small S gene of HBV is fully embedded in the polymer ...
ppt - Chair of Computational Biology
... play a variety of roles in biology. Most notably, siRNA is involved in the RNA interference (RNAi) pathway, where it interferes with the expression of a specific gene. In addition to their role in the RNAi pathway, siRNAs also act in RNAi-related pathways, e.g., as an antiviral mechanism or in shapi ...
... play a variety of roles in biology. Most notably, siRNA is involved in the RNA interference (RNAi) pathway, where it interferes with the expression of a specific gene. In addition to their role in the RNAi pathway, siRNAs also act in RNAi-related pathways, e.g., as an antiviral mechanism or in shapi ...
Lecture 13 Electrophoresis (Part-I)
... charged molecule moves to their counter charge electrodes but electric field is removed before it reaches the electrode. Movement of charged species in an electric field gives differential mobility to the sample based on the charge and consequently resolve them. Movement of the charged particle is r ...
... charged molecule moves to their counter charge electrodes but electric field is removed before it reaches the electrode. Movement of charged species in an electric field gives differential mobility to the sample based on the charge and consequently resolve them. Movement of the charged particle is r ...
DNA sentences How are proteins coded for by DNA?
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a doublestranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribon ...
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a doublestranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribon ...
DNA sentences - seed2stem.org
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a double-stranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribo ...
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a double-stranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribo ...
Molecular signatures-based prediction of enzyme
... families and superfamilies (Khersonsky et al., 2006). Namely, the conservation of structural and catalytic features observed in enzyme superfamilies such as tautomerases (Poelarends et al., 2008), enolases (Babbitt et al., 1996) and many others, suggests that enzyme specialization arose via divergen ...
... families and superfamilies (Khersonsky et al., 2006). Namely, the conservation of structural and catalytic features observed in enzyme superfamilies such as tautomerases (Poelarends et al., 2008), enolases (Babbitt et al., 1996) and many others, suggests that enzyme specialization arose via divergen ...
Learn more by reading original document
... The method used is the NJ (Neighbour Joining) method of Saitou and Nei. First you calculate distances (percent divergence) between all pairs of sequence from a multiple alignment; second you apply the NJ method to the distance matrix. 2) EXCLUDE POSITIONS WITH GAPS? With this option, any alignment p ...
... The method used is the NJ (Neighbour Joining) method of Saitou and Nei. First you calculate distances (percent divergence) between all pairs of sequence from a multiple alignment; second you apply the NJ method to the distance matrix. 2) EXCLUDE POSITIONS WITH GAPS? With this option, any alignment p ...
Cambridge Workshop November 2008
... – Authors/researchers are the experts in their fields – Limited number of FlyBase curators, and increasing amount and variety of data being published – Improves FlyBase utility for everyone ...
... – Authors/researchers are the experts in their fields – Limited number of FlyBase curators, and increasing amount and variety of data being published – Improves FlyBase utility for everyone ...
Supplementary Text S1: Detailed PCR Protocols
... Lightcycler Probe Master Mix, 0.2μM final concentration of allele specific primer set IFNB1 MF/R, and 0.1μM final concentration of Roche LNA probe #79]. The second PCR amplification was carried out using a polymerase activation at 95°C for 10 minutes, followed for 50 cycles at 94ºC for 10s, 62ºC for ...
... Lightcycler Probe Master Mix, 0.2μM final concentration of allele specific primer set IFNB1 MF/R, and 0.1μM final concentration of Roche LNA probe #79]. The second PCR amplification was carried out using a polymerase activation at 95°C for 10 minutes, followed for 50 cycles at 94ºC for 10s, 62ºC for ...
Chpt10_TxnRNAPol.doc
... accurate and efficient transcription that are not subunits of purified RNA polymerase. We will focus primarily on the general transcription initiation factors (GTIFs), which are proteins needed for accurate initiation of transcription. They are required for RNA polymerase to bind avidly and specific ...
... accurate and efficient transcription that are not subunits of purified RNA polymerase. We will focus primarily on the general transcription initiation factors (GTIFs), which are proteins needed for accurate initiation of transcription. They are required for RNA polymerase to bind avidly and specific ...