Tag-ItTM Mutation Detection Kit for CFTR 70+6
... • The Tag-It Cystic Fibrosis assay screens for the currently recommended 23 mutations and 4 variants, plus 1078delT, I148T, and 15 of the world’s most common and and North American’s prevalent mutations. The Tag-It Cystic Fibrosis B assay screens for and additional 31 mutations and 1 variant (D1270 ...
... • The Tag-It Cystic Fibrosis assay screens for the currently recommended 23 mutations and 4 variants, plus 1078delT, I148T, and 15 of the world’s most common and and North American’s prevalent mutations. The Tag-It Cystic Fibrosis B assay screens for and additional 31 mutations and 1 variant (D1270 ...
Chapter 5 - Immune Assist Micron
... acids. Polysaccharides present the highest capacity for carrying biological information since they have the greatest potential for structural variability. The amino acids in proteins and the nucleotides in nucleic acids can interconnect in only one way while the monosaccharide units in oligosacchari ...
... acids. Polysaccharides present the highest capacity for carrying biological information since they have the greatest potential for structural variability. The amino acids in proteins and the nucleotides in nucleic acids can interconnect in only one way while the monosaccharide units in oligosacchari ...
Two glucose/xylose transporter genes from the yeast Candida
... be unable to transport either glucose or xylose [9]. Ura+ transformants were first recovered in YNB maltose medium and subsequently transferred by replica plating on to medium containing different concentrations of either xylose or glucose as the sole carbon and energy source. Approx. 40 transforman ...
... be unable to transport either glucose or xylose [9]. Ura+ transformants were first recovered in YNB maltose medium and subsequently transferred by replica plating on to medium containing different concentrations of either xylose or glucose as the sole carbon and energy source. Approx. 40 transforman ...
On Periodicity in the Occurrence of Nucleotides in Protein Coding
... a genome, which are identified by locating the START and the STOP codons in the genome. There may be overlapping ORFs. To keep things simple, let us consider only prokaryotic genomes so that there are no introns ...
... a genome, which are identified by locating the START and the STOP codons in the genome. There may be overlapping ORFs. To keep things simple, let us consider only prokaryotic genomes so that there are no introns ...
Detection of genetically modified plants
... prepared outside the organism including micro-injection, macro-injection and microencapsulation; 3) Cell fusion (including protoplast fusion) or hybridisation techniques where live cells with new combinations of heritable genetic material are formed through the fusion of two or more cells by means o ...
... prepared outside the organism including micro-injection, macro-injection and microencapsulation; 3) Cell fusion (including protoplast fusion) or hybridisation techniques where live cells with new combinations of heritable genetic material are formed through the fusion of two or more cells by means o ...
Nested PCR Assays for Detection of Monilinia fructicola in Stone
... pistachio industry since the late 1980s (Michailides, 1991; Ma et al., 2001). Species identification in Botryosphaeria is complicated and difficult because the teleomorphs of these fungi are rarely encountered in nature and teleomorphic characters vary little between species. Furthermore, morphological ...
... pistachio industry since the late 1980s (Michailides, 1991; Ma et al., 2001). Species identification in Botryosphaeria is complicated and difficult because the teleomorphs of these fungi are rarely encountered in nature and teleomorphic characters vary little between species. Furthermore, morphological ...
Molecular Characterization of Visual Pigments in
... eyes and ocelli for RACE. RNA of T. longicaudatus was extracted from the compound eyes and ocelli and for B. kugenumaensis, from the whole head including the compound eyes and ocelli. Clone development was achieved by PCR using 0.5 U of Platinum Taq DNA polymerase (Invitrogen) at a final concentrati ...
... eyes and ocelli for RACE. RNA of T. longicaudatus was extracted from the compound eyes and ocelli and for B. kugenumaensis, from the whole head including the compound eyes and ocelli. Clone development was achieved by PCR using 0.5 U of Platinum Taq DNA polymerase (Invitrogen) at a final concentrati ...
Chapter 3 Transposon Mutagenesis of Rhodobacter sphaeroides
... transposition happens at a frequency of 10-4 to 10-7, it is important to eliminate the vast majority of organisms that do not have an insertion before proceeding. Finally, transposons cause very clean mutations. When an organism is mutagenized with chemicals or irradiated, it is possible to get more ...
... transposition happens at a frequency of 10-4 to 10-7, it is important to eliminate the vast majority of organisms that do not have an insertion before proceeding. Finally, transposons cause very clean mutations. When an organism is mutagenized with chemicals or irradiated, it is possible to get more ...
The Pros and Cons of Electrostatic Interactions
... At large separations or in a medium of high dipoles can rotate more or less freely, because the dipole-dipole interaction is not strong enough to align the molecules. • attractive orientation are occuring more often than repulsive ones (Boltzmann-distribution) • an angle-averaged attractive potent ...
... At large separations or in a medium of high dipoles can rotate more or less freely, because the dipole-dipole interaction is not strong enough to align the molecules. • attractive orientation are occuring more often than repulsive ones (Boltzmann-distribution) • an angle-averaged attractive potent ...
K 2
... A total of 158 samples were collected from different clinical materials (urine ,sputum, wounds and burns swabs) during the period from January to June 2012, Only 46(29.1%) isolates were identified as Klebsiella pneumonia. The molecular detecting of capsular polysaccharide gene (cps)was investigated ...
... A total of 158 samples were collected from different clinical materials (urine ,sputum, wounds and burns swabs) during the period from January to June 2012, Only 46(29.1%) isolates were identified as Klebsiella pneumonia. The molecular detecting of capsular polysaccharide gene (cps)was investigated ...
Tertiary base pair interactions in slipped loop-DNA
... sequences can assume 'unusual' forms, such as Z-, H-forms, cruciforms, etc. (1,2). As a rule, such forms do not constitute the ground state (lowest energy) conformation, but they can be stabilized either by an appropriate solution environment (high ionic strength or low pH) or superhelical stress in ...
... sequences can assume 'unusual' forms, such as Z-, H-forms, cruciforms, etc. (1,2). As a rule, such forms do not constitute the ground state (lowest energy) conformation, but they can be stabilized either by an appropriate solution environment (high ionic strength or low pH) or superhelical stress in ...
Write on zinc fingers
... a single zinc finger of Zif268 (Rebar and Pabo, 1994; Jamieson et al., 1994; Cho and Klug, 1994). There are two main methods currently used to generate engineered zinc finger arrays, modular assembly, and a bacterial selection system, and there is some debate about which method is best suited for mo ...
... a single zinc finger of Zif268 (Rebar and Pabo, 1994; Jamieson et al., 1994; Cho and Klug, 1994). There are two main methods currently used to generate engineered zinc finger arrays, modular assembly, and a bacterial selection system, and there is some debate about which method is best suited for mo ...
An economic method for the fluorescent labeling of PCR fragments
... dye label, which may be 6-carboxy-fluorescine (FAM), hexachloro-6-carboxy-fluorescine (HEX), 6-carboxy-X-rhodamine (ROX), or tetrachloro-6-carboxy-fluorescine (TET)2. These fluorescent dyes are very expensive. Labeling one primer in the 50 nmol range can cost US $100–130, depending on the dye. In or ...
... dye label, which may be 6-carboxy-fluorescine (FAM), hexachloro-6-carboxy-fluorescine (HEX), 6-carboxy-X-rhodamine (ROX), or tetrachloro-6-carboxy-fluorescine (TET)2. These fluorescent dyes are very expensive. Labeling one primer in the 50 nmol range can cost US $100–130, depending on the dye. In or ...
File S4 – Novel OR alleles in the CAST genome, Related
... implemented in the DESeq2 package (Love et al., 2014) Size factors were calculated with estimateSizeFactorsForMatrix and then used to divide the raw counts. To compare OR expression levels between datasets, normalization to account for the number of OSNs present in the WOM samples was carried out su ...
... implemented in the DESeq2 package (Love et al., 2014) Size factors were calculated with estimateSizeFactorsForMatrix and then used to divide the raw counts. To compare OR expression levels between datasets, normalization to account for the number of OSNs present in the WOM samples was carried out su ...
Unveiling the physiological and molecular basis of Mycobacterium
... non-replicating state of infecting microorganisms that enables evasion from the host’s immune system. The clinical phenotype of TB thus raises the question of whether or not M. tuberculosis latent infections are associated to the biofilm phenotype in vivo and if these multicellular structures may al ...
... non-replicating state of infecting microorganisms that enables evasion from the host’s immune system. The clinical phenotype of TB thus raises the question of whether or not M. tuberculosis latent infections are associated to the biofilm phenotype in vivo and if these multicellular structures may al ...
Pedigree vs. Genomic Inbreeding
... • Adjusting for base level of homozygosity in population • 10% homozygosity ...
... • Adjusting for base level of homozygosity in population • 10% homozygosity ...
