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Green Fluorescent Protein (GFP) Purification Kit
... One of the basic tools of modern biotechnology is DNA splicing, cutting DNA and linking it to other DNA molecules. The basic concept behind DNA splicing is to remove a functional DNA fragment—let’s say a gene—from one organism and combine it with the DNA of another organism in order to make the prot ...
... One of the basic tools of modern biotechnology is DNA splicing, cutting DNA and linking it to other DNA molecules. The basic concept behind DNA splicing is to remove a functional DNA fragment—let’s say a gene—from one organism and combine it with the DNA of another organism in order to make the prot ...
The Escherichia coli SlyD Is a Metal Ion-regulated Peptidyl
... Independently, the protein SlyD (there called WHP, wonderous histidine-rich protein) was discovered by binding to nickel ions immobilized on nitriloacetic acid-agarose (NTA) resin (9). Derived from the amino acid sequence, SlyD consists of two sequence regions, an N-terminal stretch of 146 amino aci ...
... Independently, the protein SlyD (there called WHP, wonderous histidine-rich protein) was discovered by binding to nickel ions immobilized on nitriloacetic acid-agarose (NTA) resin (9). Derived from the amino acid sequence, SlyD consists of two sequence regions, an N-terminal stretch of 146 amino aci ...
Molecular evolution of the major chemosensory gene families in
... differences in amino acid sequence and three-dimensional structure, CSPs and OBPs might be homologous (derived from a common ancestor). Alternatively, the current OBP–CSP similarity might result from convergent evolution. Although CSPs have been identified in insect chemosensory sensilla, there is n ...
... differences in amino acid sequence and three-dimensional structure, CSPs and OBPs might be homologous (derived from a common ancestor). Alternatively, the current OBP–CSP similarity might result from convergent evolution. Although CSPs have been identified in insect chemosensory sensilla, there is n ...
chapter9
... complementary copy of DNA • Transfer RNA (tRNA) contains a binding site for mRNA codon and a binding site for a specific amino acid • Ribosomal RNA (rRNA) – component of the ribosome Chapter 9 Human Heredity by Michael Cummings ©2006 Brooks/Cole-Thomson Learning ...
... complementary copy of DNA • Transfer RNA (tRNA) contains a binding site for mRNA codon and a binding site for a specific amino acid • Ribosomal RNA (rRNA) – component of the ribosome Chapter 9 Human Heredity by Michael Cummings ©2006 Brooks/Cole-Thomson Learning ...
Discovery, Structural Determination, and Putative
... protein data bank; r.m.s.d., root mean square deviation. ...
... protein data bank; r.m.s.d., root mean square deviation. ...
Felis domesticus papillomavirus, isolated from a skin lesion, is
... NCR-1 regions have a discernible E1 binding site (Lu et al., 1993 ; Sun et al., 1996). Papillomavirus NCRs also contain multiple binding sites for transcriptional regulatory factors, such as AP-1 (Chan et al., 1990), NF-1 (Apt et al., 1993), SP-1 (Gloss & Bernard, 1990), transcriptional enhancer fac ...
... NCR-1 regions have a discernible E1 binding site (Lu et al., 1993 ; Sun et al., 1996). Papillomavirus NCRs also contain multiple binding sites for transcriptional regulatory factors, such as AP-1 (Chan et al., 1990), NF-1 (Apt et al., 1993), SP-1 (Gloss & Bernard, 1990), transcriptional enhancer fac ...
3-1 Cyclin-Dependent Kinases
... in the primary sequence, changes structure to become a beta strand upon cyclin binding, also contributing to reconfiguration of the active site and T-loop. We discuss the structural basis of Cdk activation in more detail in section 3-4. First, we will describe the cyclins and other regulators that i ...
... in the primary sequence, changes structure to become a beta strand upon cyclin binding, also contributing to reconfiguration of the active site and T-loop. We discuss the structural basis of Cdk activation in more detail in section 3-4. First, we will describe the cyclins and other regulators that i ...
WW Domains Provide a Platform for the
... during 25 min with a total run time of 45 min. Data were analyzed in batch using the Mascot search engine (57), and proteins were considered “hits” if two independent peptides or a single peptide with a Mascot score of 50 or higher was found. Protein hits were converted to gene identifiers (GeneIDs) ...
... during 25 min with a total run time of 45 min. Data were analyzed in batch using the Mascot search engine (57), and proteins were considered “hits” if two independent peptides or a single peptide with a Mascot score of 50 or higher was found. Protein hits were converted to gene identifiers (GeneIDs) ...
Protein Folding Cell and Mol Biology Lab
... (see end of this file for selected text pages on CDK2/cyclin, Calmodulin, protein folding diseases, prions and chaperones Protein Structure Sites: Protein Folding (Chapt. 3 of World of the Cell) and Enzymes (Chapt. 6): How a protein folds in 3D space is important for protein function. If a protein d ...
... (see end of this file for selected text pages on CDK2/cyclin, Calmodulin, protein folding diseases, prions and chaperones Protein Structure Sites: Protein Folding (Chapt. 3 of World of the Cell) and Enzymes (Chapt. 6): How a protein folds in 3D space is important for protein function. If a protein d ...
Protein Interaction Analysis - Bio-Rad
... Even if a protein and its ligand exhibit strong binding affinity for each other and are expressed at sufficient levels in a particular tissue to interact functionally, other factors influence the nature of their interaction. Certain molecules, such as ions or cofactors, may be critical for binding, ...
... Even if a protein and its ligand exhibit strong binding affinity for each other and are expressed at sufficient levels in a particular tissue to interact functionally, other factors influence the nature of their interaction. Certain molecules, such as ions or cofactors, may be critical for binding, ...
A Guide to Finding - University of Utah
... Expasy entries provide other useful descriptive information about your POI in the “CrossReferences” section (e.g. gene ontology listings and genome annotations) and “Comments” section (e.g. function and subcellular localization). ...
... Expasy entries provide other useful descriptive information about your POI in the “CrossReferences” section (e.g. gene ontology listings and genome annotations) and “Comments” section (e.g. function and subcellular localization). ...
The ACT Domain: A Small Molecule Binding Domain
... domains. As will be seen later in this review, recently determined structures demonstrate that the ACT domain shows an increasing diversity in tertiary and quaternary architecture as well as ligand binding interactions. A novel type of ACT domain-containing protein family whose members contain ACT d ...
... domains. As will be seen later in this review, recently determined structures demonstrate that the ACT domain shows an increasing diversity in tertiary and quaternary architecture as well as ligand binding interactions. A novel type of ACT domain-containing protein family whose members contain ACT d ...
Trends in Genetics 9:375. [pdf reprint 109 kb]
... p\�1529 (Ref. 2). In our experience, the method failed with pUC8-1, pUC8-2, pUC9-2, pUC12 and pUC18 (Ref. 2). The differ ent results obtained with very similar vectors, such as pUC18 and pUC19, are probably the result of sequence differences in the amino termini of the Lacla proteins they produce; ...
... p\�1529 (Ref. 2). In our experience, the method failed with pUC8-1, pUC8-2, pUC9-2, pUC12 and pUC18 (Ref. 2). The differ ent results obtained with very similar vectors, such as pUC18 and pUC19, are probably the result of sequence differences in the amino termini of the Lacla proteins they produce; ...
inhibition of protein synthesis in cell-free systems by
... significant deacylation occurred during the incubation, then the specific activity or the total counts incorporated into the protein should be greatly lowered. From the results of this experiment, it can be seen that HCAA does inhibit the overall conversion from s-RNA to ribosomal protein, and the o ...
... significant deacylation occurred during the incubation, then the specific activity or the total counts incorporated into the protein should be greatly lowered. From the results of this experiment, it can be seen that HCAA does inhibit the overall conversion from s-RNA to ribosomal protein, and the o ...
The Diversity of Lysine-Acetylated Proteins in Escherichia coli
... modifying or regulating the activities of various enzymes involved in critical metabolic processes and the synthesis of building blocks in response to environmental changes. Keywords: Escherichia coli ...
... modifying or regulating the activities of various enzymes involved in critical metabolic processes and the synthesis of building blocks in response to environmental changes. Keywords: Escherichia coli ...
Abstract - Plant Sulfur Network
... and metG genes. In these mutants a conserved N-terminal phenylalanine (F48) of MetR is changed to serine or leucine. Mutated MetR(F48S) may be more resistant to degradation. Thus, the efficient synthesis of homocysteine (via homocysteine synthase) can be achieved by three different classes of mutati ...
... and metG genes. In these mutants a conserved N-terminal phenylalanine (F48) of MetR is changed to serine or leucine. Mutated MetR(F48S) may be more resistant to degradation. Thus, the efficient synthesis of homocysteine (via homocysteine synthase) can be achieved by three different classes of mutati ...
Document
... highly polymorphic S-RNase gene. To date, the mechanism generating the allelic diversity of this gene is largely unknown. Natural populations offer a good opportunity to address this question because they likely contain different alleles that share recent common progenitors. We identified 19 S haplo ...
... highly polymorphic S-RNase gene. To date, the mechanism generating the allelic diversity of this gene is largely unknown. Natural populations offer a good opportunity to address this question because they likely contain different alleles that share recent common progenitors. We identified 19 S haplo ...
DNA
... located on chromosomes, the two components of chromosomes—DNA and protein—became candidates for the genetic material. The role of DNA in heredity was first discovered by studying bacteria and the viruses that infect them. The discovery of the genetic role of DNA began with research by Frederick ...
... located on chromosomes, the two components of chromosomes—DNA and protein—became candidates for the genetic material. The role of DNA in heredity was first discovered by studying bacteria and the viruses that infect them. The discovery of the genetic role of DNA began with research by Frederick ...
Trends in Plant Science
... that regulates SAR by identifying mutants per- tion of the reporter after INA treatment, which interact directly with NIM1/NPR1. Xinnian turbed in this response. The recent description they called npr1 mutants (non-inducer of PR Dong’s group recently described their success of a suppressor mutation ...
... that regulates SAR by identifying mutants per- tion of the reporter after INA treatment, which interact directly with NIM1/NPR1. Xinnian turbed in this response. The recent description they called npr1 mutants (non-inducer of PR Dong’s group recently described their success of a suppressor mutation ...
Chapter 1 The Framework of Biology
... The genetic material of prokaryotes is in the form of a circular DNA along with smaller pieces called plasmids. Many prokaryotes have flagella which propel them through their environment. Some prokaryotes have a slimy sheath on the outside of their capsule. Being small solves many problems. Gas diff ...
... The genetic material of prokaryotes is in the form of a circular DNA along with smaller pieces called plasmids. Many prokaryotes have flagella which propel them through their environment. Some prokaryotes have a slimy sheath on the outside of their capsule. Being small solves many problems. Gas diff ...
Metabolism: the Degradation and Synthesis of Living Cells
... phosphorylated intermediates (Nobel Prize in 1929). ...
... phosphorylated intermediates (Nobel Prize in 1929). ...
Expressway™ Cell-Free E. coli Expression System
... Successful use of the Expressway™ Cell-Free E. coli Expression System to synthesize recombinant protein requires the addition of a DNA template containing the gene of interest placed within the proper context of transcription and translation regulatory elements including a bacteriophage T7 RNA polym ...
... Successful use of the Expressway™ Cell-Free E. coli Expression System to synthesize recombinant protein requires the addition of a DNA template containing the gene of interest placed within the proper context of transcription and translation regulatory elements including a bacteriophage T7 RNA polym ...
The Genetic Code: Yesterday, Today, and Tomorrow
... (AARS). The resulting aminoacyl-tRNAs (aatRNA) are then delivered by an elongation factor (EF-Tu) to the ribosome, where by matching the anticodon of the tRNA with the triplet codon on the mRNA, the correct amino acid is added into the elongating polypeptide chain. For further details refer to S F A ...
... (AARS). The resulting aminoacyl-tRNAs (aatRNA) are then delivered by an elongation factor (EF-Tu) to the ribosome, where by matching the anticodon of the tRNA with the triplet codon on the mRNA, the correct amino acid is added into the elongating polypeptide chain. For further details refer to S F A ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.