Amino Acid Sequence and Antigenicity of the Amino
... Antigenically active regions of proteins usually contained polar residues which are located on the surface of the native molecule (Walter et al., 1980). The sequence described here contained a combination of hydrophobic and hydrophilic amino acids and its immunogenicity could not be predicted. There ...
... Antigenically active regions of proteins usually contained polar residues which are located on the surface of the native molecule (Walter et al., 1980). The sequence described here contained a combination of hydrophobic and hydrophilic amino acids and its immunogenicity could not be predicted. There ...
Genome-wide analysis of the GRAS gene family in
... (Helariutta et al., 2000). Medicago NSP1 functions as a primary transcriptional regulator mediating all known Nod factor-induced transcriptional responses and thus regulating the rhizobium-legume symbiosis (Smit et al., 2005). The function of the SHR-C proteins is as yet unknown. Physic nut has six ...
... (Helariutta et al., 2000). Medicago NSP1 functions as a primary transcriptional regulator mediating all known Nod factor-induced transcriptional responses and thus regulating the rhizobium-legume symbiosis (Smit et al., 2005). The function of the SHR-C proteins is as yet unknown. Physic nut has six ...
XBRAINоаWhey Protein Isolate Worlds finest Only the Finest Quality
... percent of the protein content. Whey as a group of proteins is the fraction that separates out during cheese making. This fraction is purified to different concentrations depending on the endproduct desired and varies in protein, lactose, immunoglobulins, minerals and fat content. Of all protein ...
... percent of the protein content. Whey as a group of proteins is the fraction that separates out during cheese making. This fraction is purified to different concentrations depending on the endproduct desired and varies in protein, lactose, immunoglobulins, minerals and fat content. Of all protein ...
Anti-c-myc antibody 9E10 - Protein Engineering, Design and Selection
... (framework IV—KLEIK) of the scFv. The gene for the antibody fragment was recloned then into pHEN 1 (Hoogenboom et al., 1991) using SfiI and EcoRI for digestion. The mutant constructs were transformed into Escherichia coli TG 1 cells and screened for expression of the scFv product. The correct nucleo ...
... (framework IV—KLEIK) of the scFv. The gene for the antibody fragment was recloned then into pHEN 1 (Hoogenboom et al., 1991) using SfiI and EcoRI for digestion. The mutant constructs were transformed into Escherichia coli TG 1 cells and screened for expression of the scFv product. The correct nucleo ...
Function and specificity of 14-3-3 proteins in the regulation of
... reduced in vitro by 5¢-AMP which interacts with 14-3-3s and directly reduces their binding to target ligands (Kaiser and Spill, 1991; Athwal et al., 1998b). It is noteworthy that there is evidence for an AMP binding site in a spinach 14-3-3 isoform. Thus 14-3-3 protein activity might also be under m ...
... reduced in vitro by 5¢-AMP which interacts with 14-3-3s and directly reduces their binding to target ligands (Kaiser and Spill, 1991; Athwal et al., 1998b). It is noteworthy that there is evidence for an AMP binding site in a spinach 14-3-3 isoform. Thus 14-3-3 protein activity might also be under m ...
Interpro - European Bioinformatics Institute
... By uniting the member databases, InterPro capitalises on their individual strengths, producing a powerful diagnostic tool & integrated database ...
... By uniting the member databases, InterPro capitalises on their individual strengths, producing a powerful diagnostic tool & integrated database ...
Characterizing the Grape Transcriptome
... We report the analysis and annotation of 146,075 expressed sequence tags from Vitis species. The majority of these sequences were derived from different cultivars of Vitis vinifera, comprising an estimated 25,746 unique contig and singleton sequences that survey transcription in various tissues and ...
... We report the analysis and annotation of 146,075 expressed sequence tags from Vitis species. The majority of these sequences were derived from different cultivars of Vitis vinifera, comprising an estimated 25,746 unique contig and singleton sequences that survey transcription in various tissues and ...
The Feedbag - Hoober Feeds
... amino acid level is what is really important milk it gets from its mother. If you buy a bag and not the protein level. The dairy of cheap milk replacer and do an amino nutrition industry is relatively new to the acid analysis of that milk replacer you will idea but is rapidly adopting it but it has ...
... amino acid level is what is really important milk it gets from its mother. If you buy a bag and not the protein level. The dairy of cheap milk replacer and do an amino nutrition industry is relatively new to the acid analysis of that milk replacer you will idea but is rapidly adopting it but it has ...
Constitutive expression of RyhB regulates the heme biosynthesis
... In this study, we found that the constitutive overexpression of natural small RNA RyhB influenced the transcription of iron-containing enzymes and drove the metabolic flux to ALA production. Interestingly, overexpression of ryhB down-regulated the transcription of hemB and hemH. The presence of poss ...
... In this study, we found that the constitutive overexpression of natural small RNA RyhB influenced the transcription of iron-containing enzymes and drove the metabolic flux to ALA production. Interestingly, overexpression of ryhB down-regulated the transcription of hemB and hemH. The presence of poss ...
Construction of a new cloning vector utilizing a cryptic plasmid and
... Ts-resistance gene on pSY10, was determined and deposited in the DNA Data Bank of Japan ...
... Ts-resistance gene on pSY10, was determined and deposited in the DNA Data Bank of Japan ...
Folie 1 - FLI
... similarities, aligning sequences with structures, modeling of rigid body shifts, distortions, loops and side chains, as well as detecting errors in a model. Despite these problems, it is currently possible to model with useful accuracy significant parts of approximately one third of all known protei ...
... similarities, aligning sequences with structures, modeling of rigid body shifts, distortions, loops and side chains, as well as detecting errors in a model. Despite these problems, it is currently possible to model with useful accuracy significant parts of approximately one third of all known protei ...
doc - DePaul University
... the hydrophobic core and results suggested that the core amino acids are likely responsible for the overall stability of the protein while the non-core amino acids play a significant role in determining the tertiary structure. The results were encouraging, demonstrating that it was possible to repac ...
... the hydrophobic core and results suggested that the core amino acids are likely responsible for the overall stability of the protein while the non-core amino acids play a significant role in determining the tertiary structure. The results were encouraging, demonstrating that it was possible to repac ...
Purification and proteomic characterization of plastids from Brassica
... Plastids are functionally and structurally diverse organelles responsible for numerous biosynthetic reactions within the plant cell. Plastids from embryos have a range of properties depending upon the plant source but compared to other plastid types are poorly understood and therefore, we term them ...
... Plastids are functionally and structurally diverse organelles responsible for numerous biosynthetic reactions within the plant cell. Plastids from embryos have a range of properties depending upon the plant source but compared to other plastid types are poorly understood and therefore, we term them ...
Analysis of Two Genes Encoding Prothrombin Activators in
... can be assisted by the construction of the firefly luciferase expression assay, where intron 2 can be used as the cis-element contributing to the regulation for reporter gene expression. Next, in recognising for any important upstream promoter element, a 5’ deletion study can be performed on the who ...
... can be assisted by the construction of the firefly luciferase expression assay, where intron 2 can be used as the cis-element contributing to the regulation for reporter gene expression. Next, in recognising for any important upstream promoter element, a 5’ deletion study can be performed on the who ...
Biotechnology and bioengineering
... of the valuable long-chain polyunsaturated fatty acid (LCPUFA) arachidonic acid within triacylglycerols in cytoplasmic lipid bodies. Here, we describe cloning and mutagenesis of the P. incisa acetohydroxyacid synthase (PiAHAS) gene for use as an herbicide resistance selection marker for transformati ...
... of the valuable long-chain polyunsaturated fatty acid (LCPUFA) arachidonic acid within triacylglycerols in cytoplasmic lipid bodies. Here, we describe cloning and mutagenesis of the P. incisa acetohydroxyacid synthase (PiAHAS) gene for use as an herbicide resistance selection marker for transformati ...
Proteome of amyloplasts isolated from
... stage. While our understanding of what is considered their primary function, i.e. the biosynthesis and degradation of starch, has increased dramatically in recent years, relatively little is known about other biochemical processes taking place in these organelles. To help fill this gap, a proteomic a ...
... stage. While our understanding of what is considered their primary function, i.e. the biosynthesis and degradation of starch, has increased dramatically in recent years, relatively little is known about other biochemical processes taking place in these organelles. To help fill this gap, a proteomic a ...
C7orf30 is necessary for biogenesis of the large
... cellular respiration. Many mutations in mtDNA-encoded genes for the RNA components of the mitochondrial translation apparatus (mt-rRNAs and mt-tRNAs) have been identified, and detection of yet uncharacterized mutations is relatively straightforward. On the contrary, a group of mutations in nuclear ge ...
... cellular respiration. Many mutations in mtDNA-encoded genes for the RNA components of the mitochondrial translation apparatus (mt-rRNAs and mt-tRNAs) have been identified, and detection of yet uncharacterized mutations is relatively straightforward. On the contrary, a group of mutations in nuclear ge ...
Synaptonemal complex proteins - Journal of Cell Science
... logues, it may provide a mechanism for positive recombination interference. Immunogold epitope mapping of antibodies to four Syn1 fusion proteins places the amino terminus of Syn1 towards the centre of the synaptonemal complex while the carboxyl terminus extends well into the lateral domain of the s ...
... logues, it may provide a mechanism for positive recombination interference. Immunogold epitope mapping of antibodies to four Syn1 fusion proteins places the amino terminus of Syn1 towards the centre of the synaptonemal complex while the carboxyl terminus extends well into the lateral domain of the s ...
MALDI Imaging of Formalin-Fixed Paraffin
... humans, R-enolase, β-Actin, Lasp-1, and neurofilament triplet L and M are also down-regulated.3–6 In contrast, human peroxiredoxin 2, complexin I, fatty acid binding protein, L type calcium channel d subunit, mitochondrial complex III, and ATP synthase D chain are up-regulated.5 These results have b ...
... humans, R-enolase, β-Actin, Lasp-1, and neurofilament triplet L and M are also down-regulated.3–6 In contrast, human peroxiredoxin 2, complexin I, fatty acid binding protein, L type calcium channel d subunit, mitochondrial complex III, and ATP synthase D chain are up-regulated.5 These results have b ...
Lecture 5 PP
... The selection of the correct amino acid must be highly accurate or the polypeptides may be nonfunctional Error rate is less than one in every 100,000 Sequences throughout the tRNA including but not limited to the anticodon are used as recognition sites ...
... The selection of the correct amino acid must be highly accurate or the polypeptides may be nonfunctional Error rate is less than one in every 100,000 Sequences throughout the tRNA including but not limited to the anticodon are used as recognition sites ...
The Antagonist Function of Arabidopsis WRKY53 and
... npr1-1 treated with SA but completely blocked in the jar1-1 mutant and constitutively high in coil-1 (Figure 2B). These results demonstrate that ESR is a target of the JAR1-mediated and COI1-mediated JA signaling pathways and is downstream of JAR1 and COI1. SA signaling on ESR and WRKY53 expression ...
... npr1-1 treated with SA but completely blocked in the jar1-1 mutant and constitutively high in coil-1 (Figure 2B). These results demonstrate that ESR is a target of the JAR1-mediated and COI1-mediated JA signaling pathways and is downstream of JAR1 and COI1. SA signaling on ESR and WRKY53 expression ...
440-kD Ankyrins: Structure of the Major
... KYRINS are a family of spectrin-binding proteins that link the spectrin/actin network to cytoplasmic domains of integral proteins that include ion channels and cell adhesion molecules (Bennett, 1992; Bennett and Giliigan, 1993; Davis et al., 1993). Ankyrins contain three structural domains: (a) an N ...
... KYRINS are a family of spectrin-binding proteins that link the spectrin/actin network to cytoplasmic domains of integral proteins that include ion channels and cell adhesion molecules (Bennett, 1992; Bennett and Giliigan, 1993; Davis et al., 1993). Ankyrins contain three structural domains: (a) an N ...
Translation Tutorial
... A process called transcription starts in the nucleus, where an enzyme called RNA polymerase splits the DNA molecule. Next, free floating mRNA nucleotides bond to the open DNA molecule. next Once finished, the mRNA breaks away and exits the nucleus. The mRNA will then join a ribosome. Now, the proces ...
... A process called transcription starts in the nucleus, where an enzyme called RNA polymerase splits the DNA molecule. Next, free floating mRNA nucleotides bond to the open DNA molecule. next Once finished, the mRNA breaks away and exits the nucleus. The mRNA will then join a ribosome. Now, the proces ...
A novel approach for protein subcellular location prediction using
... the protein as a proxy for location based on the hypothesis that the physicochemical properties of the residues of a protein must be somehow coupled to the physicochemical properties of the environment where the protein performs its function; therefore the differences between environments will be im ...
... the protein as a proxy for location based on the hypothesis that the physicochemical properties of the residues of a protein must be somehow coupled to the physicochemical properties of the environment where the protein performs its function; therefore the differences between environments will be im ...
TAK1-binding protein 1 is a pseudophosphatase
... kinase cascades. In the current study we report the crystal structure of the N-terminal domain of TAB1. Surprisingly, TAB1 possesses a fold closely related to that of the PPM (Mg2+ - or Mn2+ dependent protein phosphatase) family as demonstrated by the close structural similarity with protein phospha ...
... kinase cascades. In the current study we report the crystal structure of the N-terminal domain of TAB1. Surprisingly, TAB1 possesses a fold closely related to that of the PPM (Mg2+ - or Mn2+ dependent protein phosphatase) family as demonstrated by the close structural similarity with protein phospha ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.