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Systems-wide Chicken DNA Microarrays, Gene Expression Profiling
... selected for extremes in growth rate (Ricard, 1975; Remignon et al., 1994) [fast-growing line (FGL) or slow-growing line, (SGL)] or body composition at a similar growth rate (Leclercq et al., 1980; Leclercq, 1988) [fat line (FL) or lean line (LL)]. In FL and LL chickens, selection for seven generati ...
... selected for extremes in growth rate (Ricard, 1975; Remignon et al., 1994) [fast-growing line (FGL) or slow-growing line, (SGL)] or body composition at a similar growth rate (Leclercq et al., 1980; Leclercq, 1988) [fat line (FL) or lean line (LL)]. In FL and LL chickens, selection for seven generati ...
A Toc75-like protein import channel is abundant in chloroplasts
... and AY 040053. All peptides obtained by either Edman degradation or mass spectrometry showed sequence similarity to the EST clones (Figure 2). Both AF 360205 and AY 040053 locate to the same gene on chromosome V in Arabidopsis, at5g19620. The polypeptide deduced from the EST sequences has a length o ...
... and AY 040053. All peptides obtained by either Edman degradation or mass spectrometry showed sequence similarity to the EST clones (Figure 2). Both AF 360205 and AY 040053 locate to the same gene on chromosome V in Arabidopsis, at5g19620. The polypeptide deduced from the EST sequences has a length o ...
Heterogeneity of Genome and Proteome Content in Bacteria
... the archaea and eukaryota putatively share many genes and proteins involved in information and cellular activity, whereas archaea and bacteria share many ...
... the archaea and eukaryota putatively share many genes and proteins involved in information and cellular activity, whereas archaea and bacteria share many ...
An NIH funded center led by deCODE biostructures and
... Research Institute has been established to integrate innovative technological advances in structural biology as part of the NIGMS Protein Structure Initiative. The work of the Accelerated Technologies Center for Gene to 3D Structure (www.atcg3d.org) is focused on the development, operation and deplo ...
... Research Institute has been established to integrate innovative technological advances in structural biology as part of the NIGMS Protein Structure Initiative. The work of the Accelerated Technologies Center for Gene to 3D Structure (www.atcg3d.org) is focused on the development, operation and deplo ...
Serial Endosymbiotic Theory
... Chloroplasts originated when symbiosis between small autotrophic bacteria and larger prokaryotes resulted in the incorporation of the smaller autotrophic prokaryote into the biological system of the larger prokaryote (Campbell and Reece 2005). The α-proteobacteria, such as Paracoccus, Rickettsia, an ...
... Chloroplasts originated when symbiosis between small autotrophic bacteria and larger prokaryotes resulted in the incorporation of the smaller autotrophic prokaryote into the biological system of the larger prokaryote (Campbell and Reece 2005). The α-proteobacteria, such as Paracoccus, Rickettsia, an ...
Serial Endosymbiotic Theory
... (mitochondria) to an ancestor (prokaryote) and a feasible mechanism to convert the existing structures from the ancestral state. Ribosomes and SET Within mitochondria are ribosomes that were assumed to be similar to bacterial ribosomes in size and structure. The structure and composition of mitochon ...
... (mitochondria) to an ancestor (prokaryote) and a feasible mechanism to convert the existing structures from the ancestral state. Ribosomes and SET Within mitochondria are ribosomes that were assumed to be similar to bacterial ribosomes in size and structure. The structure and composition of mitochon ...
Recommendations for Riboprobe Synthesis
... Recommendations for Riboprobe Synthesis by In Vitro Transcription Probe contructs should be made of species-specific homologs to match the tissue; mouse on mouse, human on human, etc. When preparing constructs for riboprobe synthesis, select a region of the gene of interest that is 200 500 bases in ...
... Recommendations for Riboprobe Synthesis by In Vitro Transcription Probe contructs should be made of species-specific homologs to match the tissue; mouse on mouse, human on human, etc. When preparing constructs for riboprobe synthesis, select a region of the gene of interest that is 200 500 bases in ...
Periodicities in Sequence Residue Hydropathy and the Implications on Protein Folds
... protein’s structure from its amino acid sequence is becoming increasingly important. Currently, discounting homologies of over 35% identity, there are over 40,000 protein sequences identified, and yet only 4200 experimentally-determined protein structures. Being able to predict proteins structure fr ...
... protein’s structure from its amino acid sequence is becoming increasingly important. Currently, discounting homologies of over 35% identity, there are over 40,000 protein sequences identified, and yet only 4200 experimentally-determined protein structures. Being able to predict proteins structure fr ...
Chromium Incorporated in RNA and DNA
... were significantly greater than bound to RNA and was 5.12 ð 0.30/1000 and 1.80 ð 0.11/1000 nucleotides for 10 µm potassium dichromate or chromium chloride and 7.74 ð 0.44/1000 nucleotides and 3.70 ð 0.25 for 100 µm respectively. Under the same conditions to RNA 3.86 ð 0.45 Cr atoms/ 1000 nucleotides ...
... were significantly greater than bound to RNA and was 5.12 ð 0.30/1000 and 1.80 ð 0.11/1000 nucleotides for 10 µm potassium dichromate or chromium chloride and 7.74 ð 0.44/1000 nucleotides and 3.70 ð 0.25 for 100 µm respectively. Under the same conditions to RNA 3.86 ð 0.45 Cr atoms/ 1000 nucleotides ...
A new type of Hidden Markov Models to predict complex domain
... distinction may be unrelevant from the computational view-point, we term both types of regions with the more general word ”unit”. However, the majority of proteins are multi-domains, i.e., composed of several domains. It is known that the relative order of these units may be altered by domain swapin ...
... distinction may be unrelevant from the computational view-point, we term both types of regions with the more general word ”unit”. However, the majority of proteins are multi-domains, i.e., composed of several domains. It is known that the relative order of these units may be altered by domain swapin ...
INF380 – Proteomics
... There are several reasons for doing mass spectrometry on peptides, and not (solely) on intact proteins. – The absolute error in the measurement increases with the measured ...
... There are several reasons for doing mass spectrometry on peptides, and not (solely) on intact proteins. – The absolute error in the measurement increases with the measured ...
Familial juvenile hyperuricemic nephropathy: Detection of mutations
... and chronic renal failure. More than 50 families in various ethnic groups have been described since Duncan and Dixon first noted the disease in 1960 [1]. Affected family members show the impairment of urate excretion before puberty and usually develop hyperuricemia and gout after adolescence [2]. Re ...
... and chronic renal failure. More than 50 families in various ethnic groups have been described since Duncan and Dixon first noted the disease in 1960 [1]. Affected family members show the impairment of urate excretion before puberty and usually develop hyperuricemia and gout after adolescence [2]. Re ...
Protein Structure
... The hydrophobic effect is central to protein folding. Each protein folds into the shape that maximizes the number of noncovalent bonds that both it and the water around it can form. A key factor in this process is the segregation of nonpolar side chains of the protein away from the polar groups of t ...
... The hydrophobic effect is central to protein folding. Each protein folds into the shape that maximizes the number of noncovalent bonds that both it and the water around it can form. A key factor in this process is the segregation of nonpolar side chains of the protein away from the polar groups of t ...
Ribosomes and Protein Synthesis
... at the same time that the microsomal fraction is the site of polypeptide polymerization (18, 46) . However, the term microsome is only an operational defmition of a high-speed, multicomponent, sedimentable cell fraction, and soon it became clear that the ribonucleoprotein particles of the microsome ...
... at the same time that the microsomal fraction is the site of polypeptide polymerization (18, 46) . However, the term microsome is only an operational defmition of a high-speed, multicomponent, sedimentable cell fraction, and soon it became clear that the ribonucleoprotein particles of the microsome ...
Binding of a Growth Hormone- Inducible Nuclear Factor Is Mediated
... have features in common with activation of gene expressionby these hormones.Thus, the GH-mediated pathway of signal transduction may diverge into p91dependentand -independentpathways. There are other proteinsimmunologicallyrelated to p91 that serve similar roles in signaling pathways for cytokines; ...
... have features in common with activation of gene expressionby these hormones.Thus, the GH-mediated pathway of signal transduction may diverge into p91dependentand -independentpathways. There are other proteinsimmunologicallyrelated to p91 that serve similar roles in signaling pathways for cytokines; ...
TNT SP6 High-Yield Wheat Germ Protein Expression
... prepared from an optimized wheat germ extract and contains all the components (tRNA, ribosomes, amino acids, polymerase, and translation initiation, elongation and termination factors) necessary for protein synthesis directly from DNA templates. The TnT® SP6 High-Yield Wheat Germ Protein Expression ...
... prepared from an optimized wheat germ extract and contains all the components (tRNA, ribosomes, amino acids, polymerase, and translation initiation, elongation and termination factors) necessary for protein synthesis directly from DNA templates. The TnT® SP6 High-Yield Wheat Germ Protein Expression ...
Fatty acid - St John Brebeuf
... Concept 5.4: Proteins include a diversity of structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry mass of most cells • Protein functions include structural support, ...
... Concept 5.4: Proteins include a diversity of structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry mass of most cells • Protein functions include structural support, ...
Transcript
... degraded and replaced by DNA, through the activity of one type of DNA polymerase. Slide 8 Once an RNA primer has formed, synthesis of a new strand of DNA may begin. Remember that in the semi-conservative replication of DNA, each strand of the original DNA molecule acts as a template for the formatio ...
... degraded and replaced by DNA, through the activity of one type of DNA polymerase. Slide 8 Once an RNA primer has formed, synthesis of a new strand of DNA may begin. Remember that in the semi-conservative replication of DNA, each strand of the original DNA molecule acts as a template for the formatio ...
Supplemental Information
... fraction of the resulting RNA strands that contained the NAD modification. We observed that ~3% of the transcripts generated by this procedure were linked to NAD. In Vitro Transcription. In vitro transcription reactions contained E. coli RNA polymerase (Epicentre Biotechnologies) in 50 mM Tris-HCl, ...
... fraction of the resulting RNA strands that contained the NAD modification. We observed that ~3% of the transcripts generated by this procedure were linked to NAD. In Vitro Transcription. In vitro transcription reactions contained E. coli RNA polymerase (Epicentre Biotechnologies) in 50 mM Tris-HCl, ...
Intracellular Distribution of Radioactivity in Nucleic Acid tration of
... report factors of 10—38for similar cases. Brown tensive study of @32 specffic activity-time relation and co-workers (9), using labeled adenine, ne ships in cell fractions of desoxynibonucleic acid (DNA), RNA, phosphoproteins, and inorganic ported an incorporation ratio for liven RNA :DNA phosphate ...
... report factors of 10—38for similar cases. Brown tensive study of @32 specffic activity-time relation and co-workers (9), using labeled adenine, ne ships in cell fractions of desoxynibonucleic acid (DNA), RNA, phosphoproteins, and inorganic ported an incorporation ratio for liven RNA :DNA phosphate ...
Electrophoretic_techniques2003
... sucrose or glycerol that give the sample solution density thus, allowing the sample to settle easily through the electrophoresis buffer to the bottom when injected into loading gel. ...
... sucrose or glycerol that give the sample solution density thus, allowing the sample to settle easily through the electrophoresis buffer to the bottom when injected into loading gel. ...
Signal Transduction
... enzyme, e.g., by promoting a conformational change. Alternatively, altered activity may result from binding another protein that specifically recognizes a phosphorylated domain. E.g., 14-3-3 proteins bind to domains that include phosphorylated Ser or Thr in the sequence RXXX[pS/pT]XP, where X can ...
... enzyme, e.g., by promoting a conformational change. Alternatively, altered activity may result from binding another protein that specifically recognizes a phosphorylated domain. E.g., 14-3-3 proteins bind to domains that include phosphorylated Ser or Thr in the sequence RXXX[pS/pT]XP, where X can ...
BiomedicineandLifeSciencesII_GiuseppeLAROCCA_03282007
... With the advancement of molecular biology techniques a huge amount of information on protein sequences has been made available but less information is available on structure and function of these proteins. The “ab initio” prediction of protein structure is a key instrument to better understand the p ...
... With the advancement of molecular biology techniques a huge amount of information on protein sequences has been made available but less information is available on structure and function of these proteins. The “ab initio” prediction of protein structure is a key instrument to better understand the p ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.