... A recombinant fusion protein is a protein created through genetic engineering of a fusion gene. This typically involves removing the stop codon from a DNA sequence coding for the first protein, then appending the DNA sequence of the second protein in frame through ligation or overlap extension PCR. ...
NMR-driven secondary and tertiary structure model of Ca
... (Fig. 4). Both of the fingerprint regions also contain acidic residues, which, in other EF-hand proteins, are known to interact with basic residues on the target [16]. Lastly, the fingerprint region within EF-III contains one or more Met residues, which may also be important in target interaction and ...
... (Fig. 4). Both of the fingerprint regions also contain acidic residues, which, in other EF-hand proteins, are known to interact with basic residues on the target [16]. Lastly, the fingerprint region within EF-III contains one or more Met residues, which may also be important in target interaction and ...
Biological Complexity - The University of Auckland
... One feature of biological networks that is especially important in genetic networks is the distribution of control among several different mechanisms. This is known as degeneracy. It is the ability of elements that are structurally different to perform the same function or yield the same output. Deg ...
... One feature of biological networks that is especially important in genetic networks is the distribution of control among several different mechanisms. This is known as degeneracy. It is the ability of elements that are structurally different to perform the same function or yield the same output. Deg ...
NOTIFICATION FOR CONTAINED USE ACTIVITIES INVOLVING
... Donor organism refers to the source of the genetic sequences used for modification. Vector should include all vectors and method (s) used. Modified trait can be stated as “unknown” if for example building a genomic library, Identity and function of gene(s) of donor organism responsible for the modif ...
... Donor organism refers to the source of the genetic sequences used for modification. Vector should include all vectors and method (s) used. Modified trait can be stated as “unknown” if for example building a genomic library, Identity and function of gene(s) of donor organism responsible for the modif ...
pdf
... Only tRNA’s with the amino acid esterified to the 3´ hydroxyl group of the 3´ terminal adenosine residue are utilized by the translation apparatus. The tRNA’s charged by the Class I aminoacyl tRNA synthetases must be modified, the amino acid must be moved from the 2´ hydroxyl to the 3´ hydroxyl gro ...
... Only tRNA’s with the amino acid esterified to the 3´ hydroxyl group of the 3´ terminal adenosine residue are utilized by the translation apparatus. The tRNA’s charged by the Class I aminoacyl tRNA synthetases must be modified, the amino acid must be moved from the 2´ hydroxyl to the 3´ hydroxyl gro ...
Lecture 12 Proteins Classification of protein Proteins are classified
... These are formed by the action of acids and alkalies upon protein. They are insoluble in neutral solvents. Coagulated proteins Coagulated proteins are insoluble products formed by the action of heat or alcohol on natural proteins e.g., cooked meat and cooked albumin. Secondary-derived protei ...
... These are formed by the action of acids and alkalies upon protein. They are insoluble in neutral solvents. Coagulated proteins Coagulated proteins are insoluble products formed by the action of heat or alcohol on natural proteins e.g., cooked meat and cooked albumin. Secondary-derived protei ...
Drafting Patent Claims for Filing in the United States
... A method for screening a chemical for the ability to inhibit X enzyme activity, comprising: (a) combining an enzyme according to claim 1 in a first reaction mixture with [the enzyme’s substrate(s)] under conditions in which the enzyme is capable of catalyzing the synthesis of [product]; (b) combinin ...
... A method for screening a chemical for the ability to inhibit X enzyme activity, comprising: (a) combining an enzyme according to claim 1 in a first reaction mixture with [the enzyme’s substrate(s)] under conditions in which the enzyme is capable of catalyzing the synthesis of [product]; (b) combinin ...
loading control antibodies for western blotting
... Western blotting results, and check that any increases and decreases in target proteins are actually due to experimental manipulations and not, for example, because the sample went wandering during gel loading. Internal control proteins — i.e. those with constant, unchanging levels — are usually det ...
... Western blotting results, and check that any increases and decreases in target proteins are actually due to experimental manipulations and not, for example, because the sample went wandering during gel loading. Internal control proteins — i.e. those with constant, unchanging levels — are usually det ...
Recognition of Metal Ion Binding Proteins
... Arya et al. An optimal algorithm for approximate nearest neighbor searching fixed dimensions. Journal of the ACM (JACM) (1998) vol. 45 (6) pp. 891-923 Bairoch and Apweiler. The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000. Nucleic Acids Research (2000) vol. 28 (1) pp. 45 Ca ...
... Arya et al. An optimal algorithm for approximate nearest neighbor searching fixed dimensions. Journal of the ACM (JACM) (1998) vol. 45 (6) pp. 891-923 Bairoch and Apweiler. The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000. Nucleic Acids Research (2000) vol. 28 (1) pp. 45 Ca ...
Additional file 3
... Aside from the CRD, the presence of a transmembrane region and its location along the sequence, as well as the protein orientation are also relevant to analyze those C- type lectins that function as membrane receptors. C-type lectins can be loosely classified as Type I or Type II, depending on thei ...
... Aside from the CRD, the presence of a transmembrane region and its location along the sequence, as well as the protein orientation are also relevant to analyze those C- type lectins that function as membrane receptors. C-type lectins can be loosely classified as Type I or Type II, depending on thei ...
Full Text
... and blue, respectively, indicating that all these components are glycosylated and may have cation-binding potential. PAGE under non-denaturing conditions revealed a similar gel pattern as in SDS PAGE, confirming that these proteins are highly acidic. N-terminal sequencing of the three major componen ...
... and blue, respectively, indicating that all these components are glycosylated and may have cation-binding potential. PAGE under non-denaturing conditions revealed a similar gel pattern as in SDS PAGE, confirming that these proteins are highly acidic. N-terminal sequencing of the three major componen ...
Protein Function
... variety of shapes, generally consisting of several distinct protein domains—structural units that fold more or less independently of each other. ...
... variety of shapes, generally consisting of several distinct protein domains—structural units that fold more or less independently of each other. ...
Chapter 10 - People Server at UNCW
... • A terminator sequence in the DNA indicates where the gene’s RNA-encoding region ends ...
... • A terminator sequence in the DNA indicates where the gene’s RNA-encoding region ends ...
Novel eukaryotic enzymes modifying cell
... microbial eukaryotes, and cell walls and waxes of plants and fungi. Not only do these play a major structural role in both unicellular and multicellular eukaryotes, but they are also important in the defense against parasites or interactions of parasites with their hosts. The enzymatic components of ...
... microbial eukaryotes, and cell walls and waxes of plants and fungi. Not only do these play a major structural role in both unicellular and multicellular eukaryotes, but they are also important in the defense against parasites or interactions of parasites with their hosts. The enzymatic components of ...
3`-UTR SIRF: A database for identifying clusters of short interspersed
... Background: Short (~5 nucleotides) interspersed repeats regulate several aspects of post-transcriptional gene expression. Previously we developed an algorithm (REPFIND) that assigns P-values to all repeated motifs in a given nucleic acid sequence and reliably identifies clusters of short CAC-contain ...
... Background: Short (~5 nucleotides) interspersed repeats regulate several aspects of post-transcriptional gene expression. Previously we developed an algorithm (REPFIND) that assigns P-values to all repeated motifs in a given nucleic acid sequence and reliably identifies clusters of short CAC-contain ...
Cells in culture.
... of genes simultaneously To prepare the microarray, DNA fragments —each corresponding to a gene—are spotted onto a slide by a robot. In this example, mRNA is collected from two different cell samples for a direct comparison of their relative levels of gene expression. These samples are converted to c ...
... of genes simultaneously To prepare the microarray, DNA fragments —each corresponding to a gene—are spotted onto a slide by a robot. In this example, mRNA is collected from two different cell samples for a direct comparison of their relative levels of gene expression. These samples are converted to c ...
Retroposed New Genes Out of the X in Drosophila
... from the X chromosome, it is unclear whether or not this observation is limited to the identified new genes in the group defined by 70% amino acid identity. Thus, we extended a similar analysis (see Methods) to the new retrogenes of 50% or higher identity at the amino acid level with their parental ...
... from the X chromosome, it is unclear whether or not this observation is limited to the identified new genes in the group defined by 70% amino acid identity. Thus, we extended a similar analysis (see Methods) to the new retrogenes of 50% or higher identity at the amino acid level with their parental ...
VMD training material
... Representations” box. Do you see all possible H-bonds? Why? Increase “Angle Cutoff” stepwise to 34. Why do new H-bonds appear? Discussion in class. Do you see any special order of orientation of the H-bonds? What kind of amino acids does the protein contain? What kind of function could a protein lik ...
... Representations” box. Do you see all possible H-bonds? Why? Increase “Angle Cutoff” stepwise to 34. Why do new H-bonds appear? Discussion in class. Do you see any special order of orientation of the H-bonds? What kind of amino acids does the protein contain? What kind of function could a protein lik ...
evCode-proposals - Gene Ontology Consortium
... * overexpression or ectopic expression of wild-type or mutant gene that results in aberrant behavior of the system or aberrant expression where the resulting mutant phenotype is used to make a judgment about the normal of that gene product. The IMP evidence code covers those cases when the function, ...
... * overexpression or ectopic expression of wild-type or mutant gene that results in aberrant behavior of the system or aberrant expression where the resulting mutant phenotype is used to make a judgment about the normal of that gene product. The IMP evidence code covers those cases when the function, ...
2-Oxoacid dehydrogenase multienzyme complexes
... the proposed H. volcanii operon. The corresponding number of amino acids of the protein products are given. The sequence contained within clone pNAT82 is indicated. The DNA sequence at the 5’ end of ORF1, plus the upstream region, is shown to illustrate the proposed promoter sequences (underlined), ...
... the proposed H. volcanii operon. The corresponding number of amino acids of the protein products are given. The sequence contained within clone pNAT82 is indicated. The DNA sequence at the 5’ end of ORF1, plus the upstream region, is shown to illustrate the proposed promoter sequences (underlined), ...
Enzymes - SAVE MY EXAMS!
... (a) (i) Place a cross in the box next to the letter that shows the DNA sequence which is complementary to the first four of these bases. A ...
... (a) (i) Place a cross in the box next to the letter that shows the DNA sequence which is complementary to the first four of these bases. A ...
Protein Supplements
... Nuts - 100g - 100g bag Seeds - 100g - 100g bag (6tbsps) Tofu, steamed - 250g The safest and simplest way to maintain an adequate protein intake is to eat a wide variety of foods. Many sources of vegetable protein (peas, beans, lentils and nuts) contain large amounts of carbohydrate, dietary fibre, v ...
... Nuts - 100g - 100g bag Seeds - 100g - 100g bag (6tbsps) Tofu, steamed - 250g The safest and simplest way to maintain an adequate protein intake is to eat a wide variety of foods. Many sources of vegetable protein (peas, beans, lentils and nuts) contain large amounts of carbohydrate, dietary fibre, v ...
Changes in gene expression associated with acclimation to constant
... temperatures and daily temperature cycling. Genes temperature on daily and seasonal bases. The roles played important for the maintenance of membrane integrity are by alterations in gene expression in enabling eurytherms highly responsive to temperature change. Changes in to adjust to these two temp ...
... temperatures and daily temperature cycling. Genes temperature on daily and seasonal bases. The roles played important for the maintenance of membrane integrity are by alterations in gene expression in enabling eurytherms highly responsive to temperature change. Changes in to adjust to these two temp ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.