Slide 1
... TAP tag consists of (i) calmodulin-binding peptide (ii) TEV protease cleavage site (iii) Protein A -DNA coding the TAP tag is inserted after the DNA for the protein of interest -Organism produces a recombinant protein with the TAP tag -The protein of interest is free to associate with other proteins ...
... TAP tag consists of (i) calmodulin-binding peptide (ii) TEV protease cleavage site (iii) Protein A -DNA coding the TAP tag is inserted after the DNA for the protein of interest -Organism produces a recombinant protein with the TAP tag -The protein of interest is free to associate with other proteins ...
Analysis of Cell Ageing
... Q6 – MS/MS is able to screen for many disorders at the same time, has simple preparations, requires small amount of blood, is cheap and fast and can test a large number of samples at the same time. It can also be used for drug testing and soil testing. Q7 – It is used to test for 20 other amino acid ...
... Q6 – MS/MS is able to screen for many disorders at the same time, has simple preparations, requires small amount of blood, is cheap and fast and can test a large number of samples at the same time. It can also be used for drug testing and soil testing. Q7 – It is used to test for 20 other amino acid ...
Local protein synthesis in neuronal axons: why and
... “proximal” compartment. Neuronal cell bodies and proximal neurites remain in the proximal chamber, and only the long neurites (which are likely to be axons) can pass through the silicone grease-culture dish interface under the Teflon divider into the “distal” compartments. With a complete seal, dece ...
... “proximal” compartment. Neuronal cell bodies and proximal neurites remain in the proximal chamber, and only the long neurites (which are likely to be axons) can pass through the silicone grease-culture dish interface under the Teflon divider into the “distal” compartments. With a complete seal, dece ...
Basic Array Analysis
... normalizes across regions of similar intensity. rma() uses quantile normalization, which makes each array have the same values, just in a different order ...
... normalizes across regions of similar intensity. rma() uses quantile normalization, which makes each array have the same values, just in a different order ...
91.510_ch9_2
... Errors may occur for many reasons [1] Errors in side-chain packing [2] Distortions within correctly aligned regions [3] Errors in regions of target that do not match template [4] errors in sequence alignment [5] use of incorrect templates ...
... Errors may occur for many reasons [1] Errors in side-chain packing [2] Distortions within correctly aligned regions [3] Errors in regions of target that do not match template [4] errors in sequence alignment [5] use of incorrect templates ...
7.1 The lac Operon
... • All 3 genes are transcribed together producing 1 mRNA, a polycistronic message that starts from a single promoter – Each cistron, or gene, has its own ribosome binding site – Each cistron can be translated by separate ribosomes that bind independently of each other ...
... • All 3 genes are transcribed together producing 1 mRNA, a polycistronic message that starts from a single promoter – Each cistron, or gene, has its own ribosome binding site – Each cistron can be translated by separate ribosomes that bind independently of each other ...
Post-translational Modification Learning Objective Post
... Once the protein has been synthesized by the ribosome from its corresponding mRNA in the cytosol, many proteins get directed towards the endoplasmic reticulum for further modification. Certain N and C terminal sequences are often cleaved in the ER after which they are modified by various enzymes at ...
... Once the protein has been synthesized by the ribosome from its corresponding mRNA in the cytosol, many proteins get directed towards the endoplasmic reticulum for further modification. Certain N and C terminal sequences are often cleaved in the ER after which they are modified by various enzymes at ...
extracellular polysaccharide colanic acid. cluster responsible for
... cia) was inserted into the EcoRI site. The primers used for PCR amplifications were as follows: 621 (59-ATGCCATCTAAAAAACTCTC-39) and 668 (59-AAA GAATTCTCAGCATTGCATCAACGG-39) for the left-hand end of the pPR1742 insert, 667 (59-AAAGAATTCACTGGAACGTACTCAGCC-39) and 679 (59-GGTGCATCACTGCATCCG-39) for th ...
... cia) was inserted into the EcoRI site. The primers used for PCR amplifications were as follows: 621 (59-ATGCCATCTAAAAAACTCTC-39) and 668 (59-AAA GAATTCTCAGCATTGCATCAACGG-39) for the left-hand end of the pPR1742 insert, 667 (59-AAAGAATTCACTGGAACGTACTCAGCC-39) and 679 (59-GGTGCATCACTGCATCCG-39) for th ...
AA-tRNA - Studentportalen
... I.) 43S complex includes 40S ribosomal subunit plus initiation factors eIF1, eIF1A, eIF3 and the eIF2*GTP*Met-tRNAi complex. It most probably includes eIF5 and eIF5B as well. eIF2 brings MettRNAi into the initiation complex. 2.) Formation of the complex between mRNA and the initiation factor IF4F. e ...
... I.) 43S complex includes 40S ribosomal subunit plus initiation factors eIF1, eIF1A, eIF3 and the eIF2*GTP*Met-tRNAi complex. It most probably includes eIF5 and eIF5B as well. eIF2 brings MettRNAi into the initiation complex. 2.) Formation of the complex between mRNA and the initiation factor IF4F. e ...
Heat Shock Proteins and Neurodegenerative Disorders
... Heat shock proteins (HSPs), also known as stress proteins, were first discovered in 1962 by Ritossa[1], who observed that a transient increase in temperature induced puffing patterns in the chromosomes of salivary glands of Drosophila melanogaster larvae. Temperature increases were subsequently foun ...
... Heat shock proteins (HSPs), also known as stress proteins, were first discovered in 1962 by Ritossa[1], who observed that a transient increase in temperature induced puffing patterns in the chromosomes of salivary glands of Drosophila melanogaster larvae. Temperature increases were subsequently foun ...
wrzaczek_ptms
... Post-translational modification (PTM) refers to the covalent and generally enzymatic modification of proteins during or after protein biosynthesis. (source: www.wikipedia.org) ...
... Post-translational modification (PTM) refers to the covalent and generally enzymatic modification of proteins during or after protein biosynthesis. (source: www.wikipedia.org) ...
biomolecules
... react to form organic molecules, such as amino acids. A competing theory is the RNA-world hypothesis. This hypothesis states that RNA developed first. It may have self-replicated and also served as a template for making DNA. Today, RNA serves as the intermediate between DNA and proteins. If this hypo ...
... react to form organic molecules, such as amino acids. A competing theory is the RNA-world hypothesis. This hypothesis states that RNA developed first. It may have self-replicated and also served as a template for making DNA. Today, RNA serves as the intermediate between DNA and proteins. If this hypo ...
L -2 Sample preparation Before crystallization (first step
... Does the sample contain free cysteines? Does the sample contain additives such as sodium azide, ligands, inhibitors, or substrates? Is the protein glycosylated? Is the protein phosphorylated? Is the protein N-terminal methylated? At what temperature is the protein stable? How does sample solubility ...
... Does the sample contain free cysteines? Does the sample contain additives such as sodium azide, ligands, inhibitors, or substrates? Is the protein glycosylated? Is the protein phosphorylated? Is the protein N-terminal methylated? At what temperature is the protein stable? How does sample solubility ...
Molecular and General Genetics
... be resistant to phages TC45, TuIa and K20 but sensitive to TC45hrN3. Phage adsorption experiments were performed to determine whether strain CE1265 containing pJP47 can bind irreversibly the phages TC45, TuIa or K20. However, none of these phages was bound by the cells producing the hybrid porin. Th ...
... be resistant to phages TC45, TuIa and K20 but sensitive to TC45hrN3. Phage adsorption experiments were performed to determine whether strain CE1265 containing pJP47 can bind irreversibly the phages TC45, TuIa or K20. However, none of these phages was bound by the cells producing the hybrid porin. Th ...
Food Chemistry
... (1976) method, adapted to micro-assays, uses bovine serum albumin as a standard. For evaluation, diluted samples and the Bradford reactive were placed in a 96-well micro-plate in triplicate. Absorbance was measured at 595 nm in a VersaMaxTM micro-plate reader (Molecular Devises, Sunnyvale, CA, USA). ...
... (1976) method, adapted to micro-assays, uses bovine serum albumin as a standard. For evaluation, diluted samples and the Bradford reactive were placed in a 96-well micro-plate in triplicate. Absorbance was measured at 595 nm in a VersaMaxTM micro-plate reader (Molecular Devises, Sunnyvale, CA, USA). ...
Chapter 22, Proteins
... ¾The tertiary structure of a protein is the overall conformation of a polypeptide chain caused by side-group interaction. ¾The side-groups of proteins project outward from either the helices or the sheets. Side-groups in contact with the aqueous medium tend to cause folding of the helical strands or ...
... ¾The tertiary structure of a protein is the overall conformation of a polypeptide chain caused by side-group interaction. ¾The side-groups of proteins project outward from either the helices or the sheets. Side-groups in contact with the aqueous medium tend to cause folding of the helical strands or ...
Nucleotide sequence of the 3h-terminal two
... Nucleotide sequences were initially analysed using the Genetics Computer Group (GCG) sequence analysis software package (version 7.2). The sequence was assembled with Newgelstart to initialize the GCG fragment assembly system and to support automated fragment assembly. Later, the SeqMan (DNASTAR) co ...
... Nucleotide sequences were initially analysed using the Genetics Computer Group (GCG) sequence analysis software package (version 7.2). The sequence was assembled with Newgelstart to initialize the GCG fragment assembly system and to support automated fragment assembly. Later, the SeqMan (DNASTAR) co ...
Nucleotide sequence of the 3h-terminal two
... Nucleotide sequences were initially analysed using the Genetics Computer Group (GCG) sequence analysis software package (version 7.2). The sequence was assembled with Newgelstart to initialize the GCG fragment assembly system and to support automated fragment assembly. Later, the SeqMan (DNASTAR) co ...
... Nucleotide sequences were initially analysed using the Genetics Computer Group (GCG) sequence analysis software package (version 7.2). The sequence was assembled with Newgelstart to initialize the GCG fragment assembly system and to support automated fragment assembly. Later, the SeqMan (DNASTAR) co ...
ProtocolTargetDiscoveryVDS_Spring13
... Does the enzyme work in a complex of other proteins/macromolecules or does it do its job alone? ESSENTIALITY INFO Ideally we want an enzyme that is crucial for the survival or virulence of the organism. Some of the databases have information on this however, it may be necessary to read into the jour ...
... Does the enzyme work in a complex of other proteins/macromolecules or does it do its job alone? ESSENTIALITY INFO Ideally we want an enzyme that is crucial for the survival or virulence of the organism. Some of the databases have information on this however, it may be necessary to read into the jour ...
post-transcriptional gene silencing by double
... Homology-dependent gene silencing has been recorded in various organisms, ranging from plants to animals to fungi. Because of the different experimental systems used to study this phenomenon, several terms have emerged, often describing potentially similar processes. These terms are summarized below ...
... Homology-dependent gene silencing has been recorded in various organisms, ranging from plants to animals to fungi. Because of the different experimental systems used to study this phenomenon, several terms have emerged, often describing potentially similar processes. These terms are summarized below ...
Functional analysis of an interspecies chimera of acyl carrier
... The nod genes nodF and nodE are the only specialized genes required for the production of these unusual polyunsaturated fatty acids. NodF and NodE are homologous to acyl carrier proteins and b-keto-acyl-ACP synthase (KAS) enzymes, respectively (Shearman et al. 1986; Bibb et al. 1989). KAS enzymes ar ...
... The nod genes nodF and nodE are the only specialized genes required for the production of these unusual polyunsaturated fatty acids. NodF and NodE are homologous to acyl carrier proteins and b-keto-acyl-ACP synthase (KAS) enzymes, respectively (Shearman et al. 1986; Bibb et al. 1989). KAS enzymes ar ...
How Scientists Think
... Not seeing how Sutton’s chromosomal theory could explain this curious result, Morgan suggested that perhaps it reflected uneven gamete production. Recall that Mendel assumed equal proportions of gametes in his model of factor segregation. Alterations in these proportions could, with some tortuous fu ...
... Not seeing how Sutton’s chromosomal theory could explain this curious result, Morgan suggested that perhaps it reflected uneven gamete production. Recall that Mendel assumed equal proportions of gametes in his model of factor segregation. Alterations in these proportions could, with some tortuous fu ...
Gene Finding and Sequence Annotation - Lectures For UG-5
... stop codons – TAA, TAG, TGA start codons - ATG ...
... stop codons – TAA, TAG, TGA start codons - ATG ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.