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Archaeal Transcription Initiation - IMBB
Archaeal Transcription Initiation - IMBB

... specific transcription initiation by RNA polymerase II, and eucaryal transcription initiation may be less dependent on TAFs and auxiliary transcription factors than was previously thought (Tyree et al., 1993). The archaeal TBPs have primary sequences that are z40% identical to the sequences of eucar ...
Isolation and identification of viral DNA from
Isolation and identification of viral DNA from

... Bahr, S. M., Tyler, B. C., Wooldridge, N., Butcher, B. D., Burns, T. L., Teesch, L. M., . . . Calarge, C. A. (2015). Use of the second-generation antipsychotic, risperidone, and secondary weight gain are associated with an altered gut microbiota in children. Translational Psychiatry, 5(10). doi:10.1 ...
Chapter 4
Chapter 4

Biology Single Nucleotide Polymorphisms Lab
Biology Single Nucleotide Polymorphisms Lab

... We’ve all seen television shows like CSI where an analyst injects an instrument with some unknown fluid and moments later a printer prints results full of long chemical names only the most sophisticated organic chemist could love. Indeed, these are the contents of the finest prime-time television dr ...
Your Spitting Image Guide DOC - University of Maryland School of
Your Spitting Image Guide DOC - University of Maryland School of

... DNA (deoxyribonucleic acid) is found inside the nucleus of a cell in tight bundles called chromosomes and contains all of our genetic information. This information is necessary to make a complete organism. Every cell in the human body, except red blood cells, has DNA. A person’s genetic information ...
Multiplex PCR NZYTaq 2× Green Master Mix
Multiplex PCR NZYTaq 2× Green Master Mix

DNA Profiling
DNA Profiling

... • Section of DNA on a chromosome that codes for a protein • Average around 3,000 base pairs, but can be composed of many thousands of base pairs • Humans have 30,000 genes in their 46 chromosomes – Human Genome Project: mapping the human genome, where genes are located, what proteins they code for ...
EPICENTRE Revolutionizes Cloning by Introducing CopyControl
EPICENTRE Revolutionizes Cloning by Introducing CopyControl

Gene Sequencing
Gene Sequencing

chapter 1 introduction
chapter 1 introduction

... reveal a pattern difference between DNA fragment sizes in individual organisms. Although two individuals of the same species have almost identical genomes, the differences in DNA sequence may be due to single base-pair substitutions, additions, deletions or gross chromosomal changes such as inversio ...
How to accelerate protein search on DNA: Location and dissociation
How to accelerate protein search on DNA: Location and dissociation

... In biological systems most processes start when some protein molecules bind to specific target sequences on DNA molecules to initiate a cascade of biochemical reactions.1 This fundamental aspect of protein-DNA interactions has been studied extensively by various experimental2–14 and theoretical meth ...
Divergent roles for the two PolI-like organelle DNA polymerases of
Divergent roles for the two PolI-like organelle DNA polymerases of

... These results suggest that in addition to a role in DNA replication, PolIB is also required for the repair of DNA. In that case one can predict that the DSBs induced by ciprofloxacin will accumulate to higher levels in polIb-1 plants than in WT or polIa-1 plants. This was tested by measuring the lev ...
Shark Fin Forensics
Shark Fin Forensics

... Now you can turn to the virtual lab book and compare the 12S sequences of the five unidentified shark fin DNA samples with the 12S sequence of your great white shark DNA sample. Use the following steps to organize and compare the sequences.  Organize the sequences in one place. In the lab book you ...
Electronic Supplemental Information (ESI) for Quantifying mRNA
Electronic Supplemental Information (ESI) for Quantifying mRNA

activators
activators

... Transcription Activators of Eukaryotes • The general transcription factors by themselves dictate the starting point and direction of transcription but they are capable of sponsoring only a low level of transcription or basal transcription • Transcription of active genes in cells rises above the bas ...
Genetics: The Information Broker
Genetics: The Information Broker

... C G C C G GT TAAGAT C C G ...
Biology Ch. 12
Biology Ch. 12

...  The chromatin fibers supercoil to form chromosomes that are visible in the metaphase stage of mitosis. ...
A Comparative Study on the Yield of DNA Extracted from Fresh
A Comparative Study on the Yield of DNA Extracted from Fresh

Corchorus yellow vein virus, a New World geminivirus from the Old
Corchorus yellow vein virus, a New World geminivirus from the Old

... the remaining sequence of DNA A and DNA B from the virus infecting Jute, outwardly extending specific primers (DNA A: 201For 59-TCCTCTTCGAAGAACTCCT-39, 201Rev 59-TGTATGAGCAATATCGTGAC-39; DNA B: 201BFor 59-GAAGGTATGATGTCTTCCTG-39, 201BRev 59-AATCACAATTAGCTCAAGC-39) were used in PCRs comprising a 1 ml ...
Using recombinant Cas9 nuclease to assess locus
Using recombinant Cas9 nuclease to assess locus

RNA polymerase II
RNA polymerase II

A conserved repetitive DNA element located in the centromeres of
A conserved repetitive DNA element located in the centromeres of

... segment (1). Such centromeres bind to a single microtubule and can move chromosomes of 0.26–3 megabases in size. Extensive studies also have been carried on centromeres from fission yeast (Schizosaccharomyces pombe), Drosophila melanogaster, and mammalian species. The centromeres from these species ...
DNA Technology and Genomics  I.
DNA Technology and Genomics I.

... The bacterial clone will make the protein encoded by the foreign gene. The potential uses of cloned genes fall into two general categories. a. To produce a protein product. For example, bacteria carrying the gene for human growth hormone can produce large quantities of the hormone. b. To prepare man ...
Biochemistry - Stryer - Science and Technology
Biochemistry - Stryer - Science and Technology

... sequence complete genomes: first, small genomes from viruses; then, larger genomes from bacteria; and, finally, eukaryotic genomes, including the 3-billion-base-pair human genome. Scientists are just beginning to exploit the enormous information content of these genome sequences. Finally, recombinan ...
Local DNA stretching mimics the distortion caused by - ENS-phys
Local DNA stretching mimics the distortion caused by - ENS-phys

... interstrand separation between the phosphorus atoms at the 39 extremities of the TATA sequence. While the length of the strands (the two intrastrand distances) and the separation between the 59 extremities vary only slightly, the 39–39 distance changes from 13.8 Å in B-DNA to 20.0 Å in A-DNA and fin ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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