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Nanotechnology
Nanotechnology

... material rather than as a carrier of biological information, to make structures such as two-dimensional periodic lattices (both tile-based as well as using the "DNA origami" method (DNA origami is the nanoscale folding of DNA to create arbitrary two and three dimensional shapes at the nanoscale. The ...


... Science Foundation). - - Department of Biochemistry, Ohio State University, Columbus, OH 43210. ...
Restriction Digests of DNA, Part Two
Restriction Digests of DNA, Part Two

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Strings: Theory, Properties and Applications
Strings: Theory, Properties and Applications

... 2.4.4 Design of Diagnostic Probes The task of designing a string that is able to represent a set of known sequences and, at the same time, is easily distinguishable from another set, is a problem arising in the diagnosis of viruses and bacteria in host organisms. In particular, probes are used to di ...
Gene Section NEIL1 (nei endonuclease VIII-like 1 (E. coli))
Gene Section NEIL1 (nei endonuclease VIII-like 1 (E. coli))

... activity of NEIL1: a) The Werner syndrome protein (WRN), a member of RecQ family of DNA helicases, b) Rad9, Rad1, and Hus1 as individual proteins and as the 9-1-1 complex. (7) The major DNA glycosylase for the excision of 8hydroxyguanine is OGG1. In the repair of 8ydroxyguanine by OGG1, after excisi ...
an agricultural and environmental biotechnology - Moodle
an agricultural and environmental biotechnology - Moodle

... Competency. An ephemeral state, induced by treatment with cold cations, during which bacterial cells are capable of uptaking foreign DNA. Complementary DNA or RNA. The matching strand of a DNA or RNA molecule to which its bases pair. (See DNA, RNA.) Complementary nucleotides. Members of the pairs ad ...
12–1 DNA - Cloudfront.net
12–1 DNA - Cloudfront.net

... The Double Helix ...
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... 60 Suppose an error occurs in the translation phase of gene expression. Will this error affect the DNA sequence of the gene? Why or why not? ...
RNA and Protein Synthesis
RNA and Protein Synthesis

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Structural Consequences of Modification of the Oxygen Atom of

... Although not shown, construction of the minor adduct (Adduct I) in the DNA gave similar results. In its least sterically hindered position, the short axis of the naphthyl ring in Adduct I is also perpendicular to the helical axis. Thermal Stability of NA-DNA. Thus, it was anticipated that DNA modifi ...
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... Enhanced resistance to Fusarium wilt in MCU-5 was found to be due to the action of a single major gene, designated FWRl. AFLP analyses forthe identification of DNA polymorphisms that may distinguish disease-resistant and susceptible parental and progeny plants have identified at least six DNA marker ...
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Structural determinants of DNA recognition by plant MADS

... (TFs). MADS-domain proteins typically contact their cognate binding site, the CArG-box (consensus: CCW6GG) as dimers (1). Structural analysis of animal and yeast MADS-domain protein dimers revealed that central parts of their MADS-domains form an antiparallel coiled-coil, made of two amphipathic a h ...
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... negative supercoiling, not only in relaxed DNA but also in positively supercoiled DNA (Osheroff et al, 1983; Schomburg & Grosse, 1986). As a model for this supercoiling activity, it has been suggested that the DNA is wrapped around the enzyme-DNA complex forming a gate through which the strand passa ...
Vectors and Libraries
Vectors and Libraries

... process is known as “first strand synthesis.” This hybrid molecule, however, is not useful because it is composed of both DNA and RNA. 4) Remove the RNA part of the RNA:DNA hybrids. This can be accomplished in several ways. In one method, the RNA is hydrolyzed by treatment with a strong base (sodium ...
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Cleavage of a model DNA replication fork by a Type I restriction

... hemi-methylated site would generate a hemi-methylated site and an unmethylated site, which is a target for restriction cleavage. Generation of an unmethylated site should be generally rare, but it can occur in certain mutants with replication fork crowding, as described below. Therefore, it is possi ...
Southern Blotting
Southern Blotting

12–1 DNA - cloudfront.net
12–1 DNA - cloudfront.net

... The Hershey-Chase Experiment Alfred Hershey and Martha Chase studied viruses— nonliving particles smaller than a cell that can infect living organisms. ...
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Biology Dictionary

... Competency. An ephemeral state, induced by treatment with cold cations, during which bacterial cells are capable of uptaking foreign DNA. ...
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... synthesis (polysaccharide synthesis). 12. A gene G, containing two small introns, was inserted into a yeast transposon at a position where it did not interfere with transposition. The transposon moved to new locations, and the structure of the transposon in its new locations was studied. It was foun ...
Epigenase™ 5-mC Hydroxylase TET Activity/Inhibition
Epigenase™ 5-mC Hydroxylase TET Activity/Inhibition

LAB 1: Scientific Method/Tools of Scientific Inquiry
LAB 1: Scientific Method/Tools of Scientific Inquiry

... The next exercise you will begin involves a technique you have no doubt seen on crime investigation shows such as “CSI”, though you probably did not realize what the actors were actually doing (or pretending to do!). The technique is called agarose gel electrophoresis and it is used to separate a co ...
Advanced Techniques
Advanced Techniques

... 1. Cut DNA segment into fragments, arrange based on overlapping ...
Liquid Crystal Phases: Chiral Nematic Phase
Liquid Crystal Phases: Chiral Nematic Phase

... RNA Word Hypothesis o Carl R. Woese first presented this independent RNA idea in late 1960s (Woese, C. The Genetic Code, Harper & Row, New York, 1967). o Walter Gilbert first used the phrase "RNA World" in 1986 (Gilbert, Walter, Nature, 1986) o DNA replication need proteins and enzymes while at the ...
Section 4 – Molecules
Section 4 – Molecules

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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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