3rd of 7 Review Packets
... Process is semi conservative (original strand is copied) Steps 1. Enzyme (helicase) unzip strands by breaking hydrogen bonds 2. “Spare” nucleotides are added bidirectionally to bond complementarily with use of DNA polymerases (DNA pol) 3. DNA pol only can add to the 3’ to 5’ side and new DNA is made ...
... Process is semi conservative (original strand is copied) Steps 1. Enzyme (helicase) unzip strands by breaking hydrogen bonds 2. “Spare” nucleotides are added bidirectionally to bond complementarily with use of DNA polymerases (DNA pol) 3. DNA pol only can add to the 3’ to 5’ side and new DNA is made ...
DNA Test Review What are the four nucleotides in DNA? Which
... 12. Why is tRNA important in translation? 13. What is the difference between DNA and RNA? 14. How many amino acids does this DNA sequence represent: TAAAGGCCC? 15. How can only 20 amino acids make thousands of proteins? 16. What is the ratio of A:T and C:G? 17. Why is DNA replication called semicons ...
... 12. Why is tRNA important in translation? 13. What is the difference between DNA and RNA? 14. How many amino acids does this DNA sequence represent: TAAAGGCCC? 15. How can only 20 amino acids make thousands of proteins? 16. What is the ratio of A:T and C:G? 17. Why is DNA replication called semicons ...
Chapter 10 Nucleic Acids and Protein synthesis
... Fork”. NOTE – Helicase enzymes break hydrogen bonds between bases to “unzip” DNA. DNA Polymerases – bind to the separate chains of nucleotides (1 nucleotide at a time). The polymerases build a new complimentary chain of nucleotides. NOTE – New strands are built for BOTH of the unzipped DNA chains. ...
... Fork”. NOTE – Helicase enzymes break hydrogen bonds between bases to “unzip” DNA. DNA Polymerases – bind to the separate chains of nucleotides (1 nucleotide at a time). The polymerases build a new complimentary chain of nucleotides. NOTE – New strands are built for BOTH of the unzipped DNA chains. ...
Biology 20 DNA Replication What do the initials DNA stand for
... What type of molecule is the primer? Synthesis of the new DNA strands: Once the RNA primers are in place, DNA polymerase can go to work. DNA polymerase catalyses the synthesis of the new strands: Direction of synthesis: Why? Leading strand: A different DNA polymerase will come and convert the RNA pr ...
... What type of molecule is the primer? Synthesis of the new DNA strands: Once the RNA primers are in place, DNA polymerase can go to work. DNA polymerase catalyses the synthesis of the new strands: Direction of synthesis: Why? Leading strand: A different DNA polymerase will come and convert the RNA pr ...
Lesson Outline continued
... A. The Structure of DNA 1. Genes provide directions for a cell to assemble molecules that express traits such as eye color or seed shape. ...
... A. The Structure of DNA 1. Genes provide directions for a cell to assemble molecules that express traits such as eye color or seed shape. ...
DNA - TeacherWeb
... He called this transfer “transformation”. He did not yet know about DNA and the prevailing thought of the time was that protein was the more likely culprit. ...
... He called this transfer “transformation”. He did not yet know about DNA and the prevailing thought of the time was that protein was the more likely culprit. ...
Linkage
... 1. _____________: enzyme which catalyze the _____________________________ (breaking H-Bonds) of the parental double helix. 2. Single-Strand Binding Proteins: proteins which attach and help keep the separated strands apart. 3. __________________: enzyme which relieves stress on the DNA molecule by al ...
... 1. _____________: enzyme which catalyze the _____________________________ (breaking H-Bonds) of the parental double helix. 2. Single-Strand Binding Proteins: proteins which attach and help keep the separated strands apart. 3. __________________: enzyme which relieves stress on the DNA molecule by al ...
Making A DNA Model
... 3. How many nucleotides are in one strand of your model? Does the other strand contain the same number? Explain. 4. How many of each TYPE of nucleotide (A, T, C or G) are in your DNA molecule? What do you notice about these numbers (which nucleotides MUS be equal in number)? 5. What kind of bonds ho ...
... 3. How many nucleotides are in one strand of your model? Does the other strand contain the same number? Explain. 4. How many of each TYPE of nucleotide (A, T, C or G) are in your DNA molecule? What do you notice about these numbers (which nucleotides MUS be equal in number)? 5. What kind of bonds ho ...
DNA Cornell notes
... DNA Replication: For DNA to replicate, an enzyme, helicase must first ‘unzip’ the hydrogen bonds between complementary base pairs. The site where the unzipping begins is referred to as a replication fork. The two template strands of DNA are kept separate by single stranded binding proteins. New str ...
... DNA Replication: For DNA to replicate, an enzyme, helicase must first ‘unzip’ the hydrogen bonds between complementary base pairs. The site where the unzipping begins is referred to as a replication fork. The two template strands of DNA are kept separate by single stranded binding proteins. New str ...
DNA Structure and Replication
... Primase Single priming Pol III Helicase Topoisomerase SSB (single strand DNA binding proteins) Lagging strand synthesis-discontinuous Multiple priming & synthesis 5’ -> 3’ Primase-new primer every 1-2 kb Pol III extends primers, making Okazaki fragments Pol I replaces primer Ligase joins Okazaki fra ...
... Primase Single priming Pol III Helicase Topoisomerase SSB (single strand DNA binding proteins) Lagging strand synthesis-discontinuous Multiple priming & synthesis 5’ -> 3’ Primase-new primer every 1-2 kb Pol III extends primers, making Okazaki fragments Pol I replaces primer Ligase joins Okazaki fra ...
The DNA strand that is replicated smoothly and continuously is
... 1. is a site where one DNA strand serves as a template, but the other strand is not replicated. 2. is created by the action of the enzyme RNA polymerase. 3. is only seen in prokaryotic chromosomes. 4. is a Y-shaped structure where both DNA strands are replicated simultaneously. ...
... 1. is a site where one DNA strand serves as a template, but the other strand is not replicated. 2. is created by the action of the enzyme RNA polymerase. 3. is only seen in prokaryotic chromosomes. 4. is a Y-shaped structure where both DNA strands are replicated simultaneously. ...
M220 Lecture 13 DNA is replicated by a process known as semi
... base. This can create an incorrect triplet sequence that places the wrong amino acid into the protein chain. This mutation with the substitute amino acid is called a missense mutation. The effect can be dramatic. For example, the hemoglobin found in Sickle Cell Anemia is altered in this fashion. If ...
... base. This can create an incorrect triplet sequence that places the wrong amino acid into the protein chain. This mutation with the substitute amino acid is called a missense mutation. The effect can be dramatic. For example, the hemoglobin found in Sickle Cell Anemia is altered in this fashion. If ...
DNA-Polymerase
... solution. (roughly 40 seconds-CAUTION: It bubbles quickly so do 10 second intervals) 5. Add 2.5 ml of 10x TAE buffer, then add 20 ml ethidium bromide (EtBr). 6. Gently pour solution into gel tray, remove bubbles and let it sit for 20 minutes. ...
... solution. (roughly 40 seconds-CAUTION: It bubbles quickly so do 10 second intervals) 5. Add 2.5 ml of 10x TAE buffer, then add 20 ml ethidium bromide (EtBr). 6. Gently pour solution into gel tray, remove bubbles and let it sit for 20 minutes. ...
week2
... Deoxyribonucleic Acid It’s the genetic material of organic life 4 different nucleotides They bond together in polynucleotide chain (a sequence) Bonded by a phosphodiester bond ...
... Deoxyribonucleic Acid It’s the genetic material of organic life 4 different nucleotides They bond together in polynucleotide chain (a sequence) Bonded by a phosphodiester bond ...
ecture 3: the building blocks of life
... histones, and condensed to form a structure called chromatin. • However it order for the DNA to carry out its function is must be unwound from the proteins: i.e. chromatin -> long strand of dsDNA • This dsDNA strands are shaped in the form of a “double Helix” • Each DNA strand consists of nucleotide ...
... histones, and condensed to form a structure called chromatin. • However it order for the DNA to carry out its function is must be unwound from the proteins: i.e. chromatin -> long strand of dsDNA • This dsDNA strands are shaped in the form of a “double Helix” • Each DNA strand consists of nucleotide ...
Biology 303 EXAM II 3/14/00 NAME
... the separate answer sheet, please fill-in the single best choice for each question. Please bubble-in your name on the answer sheet, leaving a space between first and last names. Also, bubble-in the last 5 digits of your social security number under “ID NUMBER” beginning in the left-most column. Good ...
... the separate answer sheet, please fill-in the single best choice for each question. Please bubble-in your name on the answer sheet, leaving a space between first and last names. Also, bubble-in the last 5 digits of your social security number under “ID NUMBER” beginning in the left-most column. Good ...
DNA - Moodle
... 4. Living organisms use DNA as their genetic material. Explain how DNA is replicated within the cells of living organisms 8 marks • helix is unwound • two strands are separated • helicase (is the enzyme that unwinds the helix separating the two strands) • by breaking hydrogen bonds between bases • ...
... 4. Living organisms use DNA as their genetic material. Explain how DNA is replicated within the cells of living organisms 8 marks • helix is unwound • two strands are separated • helicase (is the enzyme that unwinds the helix separating the two strands) • by breaking hydrogen bonds between bases • ...
Chapter Objectives:Molecular Genetics
... 38. Describe what determines whether a ribosome will be free in the cytosol or attached to rough ER 39. Explain how proteins can be targeted for specific sites within the cell 40. Describe the difference between prokaryotic and eukaryotic mRNA 41. Explain how eukaryotic mRNA is processed before it l ...
... 38. Describe what determines whether a ribosome will be free in the cytosol or attached to rough ER 39. Explain how proteins can be targeted for specific sites within the cell 40. Describe the difference between prokaryotic and eukaryotic mRNA 41. Explain how eukaryotic mRNA is processed before it l ...
Biology 303 EXAM II 3/14/00 NAME
... Which of the following clusters of terms accurately describes DNA as it is generally viewed to exist in prokaryotes and eukaryotes? A. Double-stranded, parallel, (A+T)/(C+G)= variable, (A+G)/(C+T)=1.0 B. Single-stranded, antiparallel, (A+T)/(C+G)=1.0, (A+G)/C+T)=1.0 C. Double-stranded, antiparallel, ...
... Which of the following clusters of terms accurately describes DNA as it is generally viewed to exist in prokaryotes and eukaryotes? A. Double-stranded, parallel, (A+T)/(C+G)= variable, (A+G)/(C+T)=1.0 B. Single-stranded, antiparallel, (A+T)/(C+G)=1.0, (A+G)/C+T)=1.0 C. Double-stranded, antiparallel, ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.