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pruitt_ppt_ch07
pruitt_ppt_ch07

... • Points in DNA sequence where the sequences are not identical between two or more individuals are called single nucleotide polymorphisms (SNPs) ...
Ch 20 Lecture
Ch 20 Lecture

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Ch 26 Guided Reading Key

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... – In the 1940s Erwin Chargaff, a biochemist at Columbia University, analyzed the amounts of the four bases in DNA from diverse organisms – He discovered a consistency in the equal amounts of adenine and thymine, and equal amounts of guanine and cytosine for a given species, although there was a diff ...
No Slide Title
No Slide Title

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Isolation and characterization of a functional promoter from
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... fragment in the pKA 16 clone is indicated in Fig. I. To confirm the origin of the DNA fragment and to rule out a possible major reorganization of the DNA fragment during the cloning process, the DNA fragment was amplified by PCR from genomic DNA isolated from an axenic N. europuea culture. Primers L ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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