Single Cell Biosensor coupled to Capillary Electrophoresis
... Asymmetric cyanine dyes have achieved much interest recently due to their excellent properties as non-covalent labels for nucleic acids. Upon binding to nucleic acids these dyes exhibit a very large enhancement in fluorescence intensity and have been used as fluorescent markers for DNA in various co ...
... Asymmetric cyanine dyes have achieved much interest recently due to their excellent properties as non-covalent labels for nucleic acids. Upon binding to nucleic acids these dyes exhibit a very large enhancement in fluorescence intensity and have been used as fluorescent markers for DNA in various co ...
recombinant dna lab
... the genes they contain can be activated. For example, DNA fragments may be combined with bacterial DNA so that they can later be inserted into a bacterial cell. Bacteria often contain small circular DNA molecules known as plasmids in addition to their chromosome. Scientists use restriction enzymes t ...
... the genes they contain can be activated. For example, DNA fragments may be combined with bacterial DNA so that they can later be inserted into a bacterial cell. Bacteria often contain small circular DNA molecules known as plasmids in addition to their chromosome. Scientists use restriction enzymes t ...
Lesson Plan
... make it up. Every DNA molecule is paired to another with weak hydrogen bonds. Molecules with be A will bind to those with T, and those with G will bind to C. Thus, the result is two strands of DNA bound together in a helical structure, giving DNA its nickname of double helix. What is DNA structure? ...
... make it up. Every DNA molecule is paired to another with weak hydrogen bonds. Molecules with be A will bind to those with T, and those with G will bind to C. Thus, the result is two strands of DNA bound together in a helical structure, giving DNA its nickname of double helix. What is DNA structure? ...
RECOMBINANT DNA TECHNOLOGY
... The term ‘clone’ means, exact copy of the parent. A duplicate or a look alike carrying the same genetic signature or genetic map. Cloning is the best application of recombinant DNA technology and could be applied to something as simple as DNA fragment or a larger, sophisticated mammalian specie s ...
... The term ‘clone’ means, exact copy of the parent. A duplicate or a look alike carrying the same genetic signature or genetic map. Cloning is the best application of recombinant DNA technology and could be applied to something as simple as DNA fragment or a larger, sophisticated mammalian specie s ...
Biology
... I. If the genetic material of a virus has 21% adenine, 21% guanine, 29% uracil, 29% cytosine, it is likely a single‐stranded RNA virus. II. If a double‐stranded DNA contains 32% cytosine, then the proportion of adenine in the double‐stranded DNA is 18%. III. If an mRNA has 28% adenine and it is tran ...
... I. If the genetic material of a virus has 21% adenine, 21% guanine, 29% uracil, 29% cytosine, it is likely a single‐stranded RNA virus. II. If a double‐stranded DNA contains 32% cytosine, then the proportion of adenine in the double‐stranded DNA is 18%. III. If an mRNA has 28% adenine and it is tran ...
From DNA to proteins to genetic engineering
... • DNA replicates during interphase, so that when the cell splits into two, each will have the right number of chromosomes and amount of DNA • In meiosis, the cell splits again, without re-doubling the DNA, so that eggs and sperm have only ½ the normal amount of DNA. ...
... • DNA replicates during interphase, so that when the cell splits into two, each will have the right number of chromosomes and amount of DNA • In meiosis, the cell splits again, without re-doubling the DNA, so that eggs and sperm have only ½ the normal amount of DNA. ...
Document
... cytosine, guanine • Double helix associated with proteins • "Backbone" is deoxyribose-phosphate • Strands held together by hydrogen bonds between base pairs (A-T and C-G) • Strands are antiparallel ...
... cytosine, guanine • Double helix associated with proteins • "Backbone" is deoxyribose-phosphate • Strands held together by hydrogen bonds between base pairs (A-T and C-G) • Strands are antiparallel ...
Unit 5: Hypercholesterolemia Section 1: Cholesterol A lipid that
... A lipid that forms an essential component of animal cell membranes & acts as a precursor molecule for the synthesis of other biologically important steroids. A long carbon chain with the end carbon double bonded to oxygen & to a hydroxyl (OH) to form a carboxylic acid. Fatty acids vary in length & i ...
... A lipid that forms an essential component of animal cell membranes & acts as a precursor molecule for the synthesis of other biologically important steroids. A long carbon chain with the end carbon double bonded to oxygen & to a hydroxyl (OH) to form a carboxylic acid. Fatty acids vary in length & i ...
Biopolymers
... the major and the minor grooves. In DNA the two strands are wound around each other, joined by base-pairing between each strand. The key feature of DNA is that each base can only be paired with its “complementary” base: A with G, C with T. Note that the bases are joined by hydrogen bonds (discussed ...
... the major and the minor grooves. In DNA the two strands are wound around each other, joined by base-pairing between each strand. The key feature of DNA is that each base can only be paired with its “complementary” base: A with G, C with T. Note that the bases are joined by hydrogen bonds (discussed ...
DNA to Protein - byrdistheword
... cause a genetic disorder (a point mutation where one base is exchanged for another is called a substitution) The change of a single nucleotide in a DNA template strand can lead to production of an abnormal protein ...
... cause a genetic disorder (a point mutation where one base is exchanged for another is called a substitution) The change of a single nucleotide in a DNA template strand can lead to production of an abnormal protein ...
frontiers of genetics chap13
... c) Then, the recombinant DNA is put back into a bacterial cell, where it can replicate many times as the cell reproduces, making many copies of the desired gene. This is called gene cloning. ...
... c) Then, the recombinant DNA is put back into a bacterial cell, where it can replicate many times as the cell reproduces, making many copies of the desired gene. This is called gene cloning. ...
Ways Cells Divide
... – Reproduction is clonal (no variation in offspring) • Single, circular bacterial chromosome is replicated • Replication begins at the origin of replication and proceeds in two directions to site of termination • New chromosomes are partitioned to opposite ends of the cell • Septum forms to divide t ...
... – Reproduction is clonal (no variation in offspring) • Single, circular bacterial chromosome is replicated • Replication begins at the origin of replication and proceeds in two directions to site of termination • New chromosomes are partitioned to opposite ends of the cell • Septum forms to divide t ...
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR
... In all cases DNA segments are amplified and subsequently subjected to various analyzes and studies. In a PCR reaction, the first step is the preparation of the DNA sample that is extracted from various biological sources or tissues. In PCR, the DNA or gene to be amplified is defined as "target" and ...
... In all cases DNA segments are amplified and subsequently subjected to various analyzes and studies. In a PCR reaction, the first step is the preparation of the DNA sample that is extracted from various biological sources or tissues. In PCR, the DNA or gene to be amplified is defined as "target" and ...
Written Transcript of this video lesson in English (PDF
... The cell is a fundamental unit of structure and function in living organisms, all living things are made of one or more cells, surrounded by a membrane contains the cytoplasm inside in which cell ...
... The cell is a fundamental unit of structure and function in living organisms, all living things are made of one or more cells, surrounded by a membrane contains the cytoplasm inside in which cell ...
DNA to Protein - Seabreeze High School
... Things to think About & Discuss 1. What if a mutation occurs in the DNA? Explain how could that affect the organism’s protein? 2. What if a mutation occurs in 3rd base of the codon? Will it always code for a different amino acid? Explain. ...
... Things to think About & Discuss 1. What if a mutation occurs in the DNA? Explain how could that affect the organism’s protein? 2. What if a mutation occurs in 3rd base of the codon? Will it always code for a different amino acid? Explain. ...
Genetic recombination 2012-05-09
... homologous sequences, e.g. during meiosis, DNA repair RecA dependent or similar protein Driven by DNA homology * Site-specific recombination occurs between non-homologous sequences of limitited homology, specific 15 bp sequences recognized by specific enzymes, e.g. integrase Driven by DNA-protein in ...
... homologous sequences, e.g. during meiosis, DNA repair RecA dependent or similar protein Driven by DNA homology * Site-specific recombination occurs between non-homologous sequences of limitited homology, specific 15 bp sequences recognized by specific enzymes, e.g. integrase Driven by DNA-protein in ...
Lecture
... that are spec. 7 and 9 repeat, one from mom and dad, on chrom. 1nowadays use pcr- but flanking sequence that is unique to chromo1)). Jeffreys almost ident. Typing. Now use PCR. • 1985 - first paper on PCR (Kerry Mullis) • 1988 - FBI starts DNA casework • 1991 - first STR paper ( renaming of VNTR– co ...
... that are spec. 7 and 9 repeat, one from mom and dad, on chrom. 1nowadays use pcr- but flanking sequence that is unique to chromo1)). Jeffreys almost ident. Typing. Now use PCR. • 1985 - first paper on PCR (Kerry Mullis) • 1988 - FBI starts DNA casework • 1991 - first STR paper ( renaming of VNTR– co ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.