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Marine Microplankton Ecology Reading
Marine Microplankton Ecology Reading

... B. Prokaryotes This group is comprised of the Eubacteria (commonly called bacteria) and the Archeabacteria (commonly called archea) (Figure 2). They range in size from 0.3 to 10.0 um. Prokaryotes have four major cell types: spherical, rod-shaped, spiral and comma-shaped. Prokaryotes have an outer me ...
Isolating, Cloning and Sequencing DNA
Isolating, Cloning and Sequencing DNA

... Enzymes produced by bacteria which recognise specific base sequences in double-stranded DNA called restriction sites, and hydrolyse a phosphodiester bond on both strands of the DNA at these sites ...
DNA: The Molecule of Life
DNA: The Molecule of Life

... sequence called the replication origin 2) During replication, weak hydrogen bonds that hold complementary nitrogen bases together are broken (This causes the two edges to “unzip”) with a special group of enzymes called helicases (gyrase breaks the hydrogen bonds) 3) This creates two y-shaped areas ( ...
Biotech Mini-Lab Students will model the process of using restriction
Biotech Mini-Lab Students will model the process of using restriction

... is to cut your DNA strand as closely as possible to the insulin gene sequence without cutting into the gene sequence and splice it into a bacterial plasmid. Background Information The major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes. Each enzyme recognizes a ...
DNA: The Molecule of Life
DNA: The Molecule of Life

... sequence called the replication origin 2) During replication, weak hydrogen bonds that hold complementary nitrogen bases together are broken (This causes the two edges to “unzip”) with a special group of enzymes called helicases (gyrase breaks the hydrogen bonds) 3) This creates two y-shaped areas ( ...
Protein Synthesis Review Worksheet Transcription: DNA to mRNA
Protein Synthesis Review Worksheet Transcription: DNA to mRNA

... 2. If the following were part of a DNA chain, what mRNA bases would pair with it to transcribe the DNA code onto mRNA? G-G-A-T-C-G-C-C-T-T-A-G-A-A-T-C ____________________________________ 3. If DNA is described as a double helix, how should mRNA be described? ____________________ 4. How are the accu ...
X-Sheet 2 Protein Synthesis and DNA Fingerprinting
X-Sheet 2 Protein Synthesis and DNA Fingerprinting

... Proteins are macro molecules that contain the elements carbon, hydrogen, oxygen and nitrogen and some may contain sulphur and phosphorus as well. Amino acids are the building blocks (monomers) of proteins. Amino acids are joined by peptide bonds to form peptide chains. Peptide chains join to form a ...
Review Relay 1 Cell Reproduction 1. How is mitosis and cell
Review Relay 1 Cell Reproduction 1. How is mitosis and cell

... Review Relay 3 Protein Synthesis 1. _________________________ process of making mRNA _________________________ process of copying DNA _________________________ process of assembling amino acids at the ribosome _________________________ place to find DNA in the cell _________________________ place o ...
dna hw packet - Liberty Union High School District
dna hw packet - Liberty Union High School District

... A tRNA has two important areas. The anticodon, which matches the codon on the RNA strand. Remember that codons are sets of three bases that code for a single amino acid. Make sure you color the bases of the anticodon the same color as the bases on your DNA and RNA strand - they are the same molecule ...
DNA Replication
DNA Replication

... primer that initiated the leading strand is not shown because it was replaced with DNA nucleotides earlier in its synthesis. The Okazaki fragment of the lagging strand, however, still have its RNA primer attached, because a primer must initiate each new fragment. ...
Strawberry DNA extraction lab activity
Strawberry DNA extraction lab activity

... Unlike peas, for example, or humans, for that matter, which are diploids (with two sets of chromosomes), a strawberry is an octoploid (with eight sets of chromosomes). How some strawberries evolved from diploids to octoploids is part of the story that people are trying to unravel. Many people are su ...
Chapter 7: DNA and Gel Electrophoresis Extended Objective Checklist
Chapter 7: DNA and Gel Electrophoresis Extended Objective Checklist

... b. Gel Box _____ 23. Explain how to load the digested DNA into a well ...
APDC Unit IX CC DNA Bio
APDC Unit IX CC DNA Bio

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Mitosis - Fort Bend ISD
Mitosis - Fort Bend ISD

... remains closely associated. These are called sister chromatids. Crossing-over can occur during the latter part of this stage. ...
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this lesson

... • Concept of lab on a chip – Preparation step is the only one that hasn’t been automated – Lab on a chip eliminates amplification step and separation step – Labeling and reading happen simultaneously – Requires intense computational ability ...
DNA Structure lab
DNA Structure lab

... What is DNA? DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitocho ...
Ch 8 PP
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Section 1: The Structure of DNA
Section 1: The Structure of DNA

... Section 1: The Structure of DNA ...
Figure 1: The “Central Dogma” of Biology
Figure 1: The “Central Dogma” of Biology

... 11 MotA/MotB protein pairs surround the MS ring. It is believed that these pairs, together with FliG, form an ion channel. As ions pass through the channel, conformational changes cause the MS ring to rotate, much like a waterwheel. A similar “molecular motor” is used in ATP synthesis in a mitochond ...
Document
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... • PCR is used to amplify (copy) specific DNA sequences in a complex mixture when the ends of the sequence are known • Source DNA is denatured into single strands • Two synthetic oligonucleotides complementary to the 3’ ends of the segment of interest are added in great excess to the denatured DNA, t ...
Paper Clip PCR.pub
Paper Clip PCR.pub

... colored paper clips. Tell the students that they are now going to perform two rounds of PCR using paper clips as their DNA. Each color paper clip represents one of the four nucleotides in the DNA code. Students should follow their “Paper Clip PCR” activity, with all members of the group sharing the ...
Summary of lesson
Summary of lesson

... How do sticky ends of DNA help in cloning? Sample Answers: can be repaired by ligase, can connect to other sticky ends The phrase “sticky ends” refers to the unsatisfied hydrogen bonds on the single stranded segment of DNA. This sequence will seek out a sequence to bond to in solution, then the liga ...
End of chapter 14 questions and answers from the text book
End of chapter 14 questions and answers from the text book

... for different proteins. The genetic code, however, is degenerate. Although the base sequence for AGT codes for serine, other sequences may also code for this same amino acid. There are 4 base sequences which code for amino acid glycine. These are GGA, GGC, GGG and GGT Pieces of DNA which have a sequ ...
DNA – Structure and Replication
DNA – Structure and Replication

Chemical organization of cells. Macromolecules
Chemical organization of cells. Macromolecules

... repeats – a region of dyad symmetry (fig. 16). In a double-strand DNA, the complementary sequences on one strand have the opportunity to base pair only if the strand separates from its partner. As a result a hairpin could be formed. The formation of two apposed hairpins creates a cruciform. Palindro ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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