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ppt
ppt

... •Chromatin organized: •DNA •Histones ...
DNA - hudson.edu
DNA - hudson.edu

... DNA Geek • Linus Pauling was a Caltech chemist (USA), who in 1951 had discovered the alpha helical nature of protein structure. I remember his books: Vitamin C and the Common Cold and Cancer and Vitamin C also The Nature of the Chemical Bond and protested nuclear testing ...
DNA, RNA, AND PROTEIN SYNTHESIS
DNA, RNA, AND PROTEIN SYNTHESIS

... forks form on the DNA and the process continues until all of the DNA has been replicated. • If only 1 was formed it would take too long to replicate DNA (53 days for humans!) • When replication is finished, there are 2 DNA molecules, each has one old strand and one new strand • This is called semi-c ...
DON”T KNOW
DON”T KNOW

The Molecular Genetics of Gene Expression
The Molecular Genetics of Gene Expression

... Transcription Initiation • Promoter = nucleotide sequence 20-200 bp long—is the initial binding site of RNA polymerase and transcription initiation factors ...
Genetics Test 2
Genetics Test 2

... 38) The diagram that shows how traits are passed down generations of a family is called a PEDIGREE. 39) TRANSGENIC organisms are produced when DNA of one organism is inserted into a different organism. 40) Gel electrophoresis is used to make an organism’s DNA ...
DNA notes
DNA notes

... simple to enable it to carry genetic information. (proposed by Phoebus Levene) ...
Chapter 11: Gene Technology
Chapter 11: Gene Technology

...  Bacterial enzymes that recognize and bind to specific short sequences of DNA and then cut the DNA between specific nucleotides within the sequences  Also cut is the vector – agent used to carry the gene of interest into another cell  Commonly used vectors include viruses, yeast, and plasmids – c ...
BASIC DNA
BASIC DNA

... – Regions of DNA which differ from person to person • Locus (plural = loci) – Site or location on a chromosome • Allele – Different variants which can exist at a locus • DNA Profile – The combination of alleles for an individual ...
Bio 181: Weekly Internet
Bio 181: Weekly Internet

... Go to http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/double_digests.asp Sometimes, you want to cut a DNA sample with more than one restriction enzyme. Different enzymes, however, may have different reaction conditions (temperature, [salt], etc.). 12. You want to cut your DNA with bot ...
Gene Technology
Gene Technology

... mammary cell • Dolly’s premature death in 2003, as well as her arthritis, led to speculation that her cells were not as healthy as those of a normal sheep, possibly reflecting incomplete reprogramming of the ...
Both the packaging of DNA around histone proteins, as
Both the packaging of DNA around histone proteins, as

... histones are tightly coiled and transcriptionally inactive. These changes to DNA are inherited from parent to offspring, such that while the DNA sequence is not altered, the pattern of gene expression is passed to the next generation. This type of gene regulation is called epigenetic regulation. Epi ...
Intimate Strangers
Intimate Strangers

... somewhere between molecular complexes and very simple biological entities. Viruses contain some of the structures and exhibit some of the activities that are common to organic life, but they are missing many of the others. In general, viruses are entirely composed of a single strand of genetic infor ...
DNA TECHNOLOGY AND THE HUMAN GENOME
DNA TECHNOLOGY AND THE HUMAN GENOME

... • VECTOR  A PLASMID CARRYING EXTRA GENES OTHER THAN THOSE NEEDED FOR REPLICATION AND CONJUGATION • “F” PLASMID NOT THE ONLY PLASMID • “R” PLASMID = RESISTANCE TO ANTI-BIOTICS • PRESENCE OF “R” PLASMID MAY LEAD TO “SUPERBACTERIA” ...
Unit 6 Protein Synthesis
Unit 6 Protein Synthesis

... RECAP Transcription- means to copy mRNA  nucleus  copies DNA code for one gene ...
Supplementary information (SI) Description of technique The
Supplementary information (SI) Description of technique The

... stringency washes. The enriched library DNA was subsequently eluted from the stable probe fixed to magnetic beads using a strand displacing enzyme at optimum temperature. The targeted enrichment of complex adaptor-ligated DNA libraries can be performed with multiple samples in parallel, when a sampl ...
Structure Meets Function: Quantum Prediction of Arsenic
Structure Meets Function: Quantum Prediction of Arsenic

... a6-31+G* basis set, then on a 6-311+G* basis set, and finally on aa 6-311++G** basis set. • During the optimization process, bond length constraints awere imposed to preserve the rigidity of the protein backbone aand prevent unrealistic geometry changes. • The bonds between Zn/As and its four direct ...
AP Biology (An Introduction)
AP Biology (An Introduction)

...  Detects the gene of interest  Uses a short, single stranded DNA or RNA called a ...
Lecture Notes
Lecture Notes

... considerably more complex than those of prokaryotes – eukaryotic cells have organelles → a variety of chemical environments can exist within a cell – each cell type typically has a distinct pattern of gene expression (even though the same DNA) – there is a significant portion of introns and intergen ...
You Asked for it….. - Brownsville Independent School
You Asked for it….. - Brownsville Independent School

... In transcription segments of DNA serve as template to produce complementary mRNA molecules. In translation the code carried by mRNA is fed into a ribosome "machine" and causes specific amino acids to be linked together to form a protein. ...
Unit E - Images
Unit E - Images

... affect entire body - process reverses after 36 hours ...
Media Release
Media Release

Recombinant DNA technology article
Recombinant DNA technology article

Note 7.1 - Gene to Protein
Note 7.1 - Gene to Protein

... gene found in a strand of DNA. RNA has an alphabet made up of four letters representing nitrogenous bases; A (adenine), G (guanine), U (uracil), and C (cytosine). Once again only three letters are used to code for an amino acid. There are sixty four (43) different combinations of three nitrogenous b ...
7.1-BIO-CHEM-QUIZ-NucleicAcidsIntroduction
7.1-BIO-CHEM-QUIZ-NucleicAcidsIntroduction

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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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