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Lecture
Lecture

... The smallest viruses contain only a few genes and can have an RNA or a DNA genome; the largest viruses contain hundreds of genes and have a double-stranded DNA genome. ...
DNA - NIU Department of Biological Sciences
DNA - NIU Department of Biological Sciences

... Involves an enzyme: DNA polymerase. The DNA double helix unwinds into 2 separate strands, and a new strand is build on each old one. Thus, each new DNA molecule consists of 1 old strand plus 1 new strand. This is called “semi-conservative” replication. DNA polymerase makes the new strands, using the ...
From DNA to Protein: Genotype to Phenotype Reading Assignments
From DNA to Protein: Genotype to Phenotype Reading Assignments

... acids, the genetic code is redundant; that is, there is more than one codon for certain amino acids. However, a single codon does not specify more than one amino acid. • The genetic code is degenerate but not ...
Background information on testing of the Y chromosome The
Background information on testing of the Y chromosome The

simple discontinuous buffer system for increased solution and speed
simple discontinuous buffer system for increased solution and speed

Slide 1
Slide 1

... 12.13 Gel electrophoresis sorts DNA molecules by size  Gel electrophoresis can be used to separate DNA molecules based on size as follows: 1. A DNA sample is placed at one end of a porous gel. 2. Current is applied and DNA molecules move from the negative electrode toward the positive electrode. 3 ...
Tracking bacterial DNA replication forks in vivo by pulsed field gel
Tracking bacterial DNA replication forks in vivo by pulsed field gel

... difficult and laborious. For example, the first demonstration that dnaA,s mutations affected DNA initiation required a series of hybridization experiments to show that sequential DNA synthesis occurs from oriC when cells are shifted from the non-permissive to permissive temperature (16). This same k ...
9/11
9/11

... Plus four different bases ...
DNA Fingerprinting and Civil Liberties
DNA Fingerprinting and Civil Liberties

... Congress have answered these developments with incremental expansion of DNA databases. From an intial focus on felony sex offenses in the 1990s, there are now over forty states that collect DNA samples from all felons, while four states and the federal government permit inclusion of DNA profiles from ...
pGLO Plasmid Map
pGLO Plasmid Map

... unneeded proteins, which would put the organism at a competitive disadvantage. The sugar arabinose is both a source of energy and a source of carbon. E. coli bacteria produce three enzymes (proteins) needed to digest arabinose as a food source. The genes that code for these enzymes are not expressed ...
ReeBops
ReeBops

... about all the different parts of a dog. Dogs have fur, eyes, legs, lungs, etc. Dogs are made up of many parts. Think about humans. We have lots of different parts. But do all humans look the same? Do all dogs look the same? Why is there so much variation or differences among species of animals? A ch ...
Efficient whole-genome DNA methylation analysis of the Human
Efficient whole-genome DNA methylation analysis of the Human

Chapter 7: Essentials of Molecular Biology Question: Which strand
Chapter 7: Essentials of Molecular Biology Question: Which strand

... adenine and thymine guanine and cytosine adenine and guanine ...
AP Biology
AP Biology

... Inheritance of metabolic diseases u suggested that genes coded for enzymes u each disease (phenotype) is caused by non-functional gene product Am I just the sum of my proteins? ...
Electronic organizer student example
Electronic organizer student example

... often face a difficult dilemma. In order to explain to the jury that the incriminating DNA match arose from a database search (in which the government had thousands or millions of opportunities to find a matching profile), the defendant must admit that his profile was in the database, which in many ...
Electronic Organizer Student Example
Electronic Organizer Student Example

C tudi - DNA to Darwin
C tudi - DNA to Darwin

... in the wrong place on an evolutionary tree (they may look very different when they are in fact closely-related). b. Any examples of convergent evolution could be suggested here, for example, wings in bats and birds, camera-like eyes in primates and cephalopods, streamlined body shapes in dolphins a ...
Serge Ankri - WordPress.com
Serge Ankri - WordPress.com

gene
gene

... a. Hydrogen bonds break, and the two strands separate. b. Each strand now serves as a template for a new complimentary strand. c. Nucleotides are connected and the daughter DNA molecules are formed. ...
plasmid vector
plasmid vector

... 7. The relaxosome, consisting of Y, M, l and hostencoded lHF bound to the nicked DNA in oriT is shown interacting with the coupling protein, D, which in turn interacts with B. 8. The 5’ end of the nicked strand is shown bound to a tyrosine (Y) in l, and the 3’ end is shown being associated with l in ...
Chapter 7 Notes: DNA Profiling
Chapter 7 Notes: DNA Profiling

Proceedings Template - WORD
Proceedings Template - WORD

... Our test tube now consists of DNA strands of various length with all possible routes among the five cities. But we are only interested in the routes that start with LA and end with NY. To get only the required strands, we use a technique called Polymerase Chain Reaction (PCR). PCR is an iterative pr ...
Genetics Debate - Region 11 Math And Science Teacher Partnership
Genetics Debate - Region 11 Math And Science Teacher Partnership

... Annealing: The mixture is cooled and primers bind to strands (60 °C for 1 minute) Elongation: DNA polymerase adds nucleotides to strands, producing 2 complementary strands (72 °C for 2 minutes) The procedure is repeated again! ...
1 DNA PHENOTYPING: PREDICTING ANCESTRY AND PHYSICAL
1 DNA PHENOTYPING: PREDICTING ANCESTRY AND PHYSICAL

... approaches for ancestry inference, principal component analysis and statistical clustering, both of which are performed at global and regional scales. Both require a database of reference DNA samples with well-defined ancestry, and thousands of subjects have been collected from populations around th ...
Plasmid
Plasmid

... Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype. ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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