Tool 1
... PFGE is a typing method that is widely used for foodborne bacterial pathogens such as salmonella, campylobacter, listeria, VTEC and shigella. The principle is that the bacterial genome (DNA) is cut into typically 10-20 fragments that are separated by gel electrophoresis. Different clones of bacteria ...
... PFGE is a typing method that is widely used for foodborne bacterial pathogens such as salmonella, campylobacter, listeria, VTEC and shigella. The principle is that the bacterial genome (DNA) is cut into typically 10-20 fragments that are separated by gel electrophoresis. Different clones of bacteria ...
Chromosome Structure
... Introns - May contain genes expressed independently of the exons they fall between. Many introns code for small nuclear RNAs (snoRNAs). These accumulate in the nucleolus, and may play a role in ribosome assembly. Thus the introns cut out of premRNA, may play a role in producing, or regulating produc ...
... Introns - May contain genes expressed independently of the exons they fall between. Many introns code for small nuclear RNAs (snoRNAs). These accumulate in the nucleolus, and may play a role in ribosome assembly. Thus the introns cut out of premRNA, may play a role in producing, or regulating produc ...
Nucleic Acids
... (1) The type of DNA that contains exons and introns is rRNA. (2) A “free” phosphate group is associated with the 5' end of a nucleic acid. (3) AMP and dAMP nucleotides differ in the sugar subunit that is present. a) All three statements are true. b) Two of the three statements are true. c) Only one ...
... (1) The type of DNA that contains exons and introns is rRNA. (2) A “free” phosphate group is associated with the 5' end of a nucleic acid. (3) AMP and dAMP nucleotides differ in the sugar subunit that is present. a) All three statements are true. b) Two of the three statements are true. c) Only one ...
Experiments Covered by the NIH Guidelines
... Those not in organisms or viruses. Those consisting entirely of DNA segments from a single non-chromosomal or viral DNA source Those consisting entirely of DNA from a prokaryotic host, including its indigenous plasmids or viruses, when propagated in that host (or a closely related strain of the same ...
... Those not in organisms or viruses. Those consisting entirely of DNA segments from a single non-chromosomal or viral DNA source Those consisting entirely of DNA from a prokaryotic host, including its indigenous plasmids or viruses, when propagated in that host (or a closely related strain of the same ...
RFLP Lab Report
... by restriction enzymes, “sticky” or “blunt” ends are formed. Sticky ends occur when singlestranded regions of the ends are complementary, and blunt ends occur when cut are opposite each other. The size of DNA fragments generated depends on the distance between recognition sites. Though RFLP analysis ...
... by restriction enzymes, “sticky” or “blunt” ends are formed. Sticky ends occur when singlestranded regions of the ends are complementary, and blunt ends occur when cut are opposite each other. The size of DNA fragments generated depends on the distance between recognition sites. Though RFLP analysis ...
No Slide Title
... Introns - May contain genes expressed independently of the exons they fall between. Many introns code for small nuclear RNAs (snoRNAs). These accumulate in the nucleolus, and may play a role in ribosome assembly. Thus the introns cut out of premRNA, may play a role in producing, or regulating produc ...
... Introns - May contain genes expressed independently of the exons they fall between. Many introns code for small nuclear RNAs (snoRNAs). These accumulate in the nucleolus, and may play a role in ribosome assembly. Thus the introns cut out of premRNA, may play a role in producing, or regulating produc ...
Deep Insight Section Common fragile sites and genomic instability
... growth. Large protein complexes moving at high speed along the chromosomes, and for long distances, make such processes possible. The RNA polymerase II (Pol II) enzyme, in mammalian cells, transcribes 18-72 nucleotides of DNA per second into RNA (Darzacq et al., 2007). One of the longest human loci, ...
... growth. Large protein complexes moving at high speed along the chromosomes, and for long distances, make such processes possible. The RNA polymerase II (Pol II) enzyme, in mammalian cells, transcribes 18-72 nucleotides of DNA per second into RNA (Darzacq et al., 2007). One of the longest human loci, ...
DNA Content of Nuclei andChromosome
... tinction in sq. ft) was taken to be proportional to the amount of DNA per nucleus.1 Variations in staining from slide to slide were never more than 10 per cent of the mean value for the standard kidney nuclei; such variations were compensated for by a factor of such size as to set equal the mean val ...
... tinction in sq. ft) was taken to be proportional to the amount of DNA per nucleus.1 Variations in staining from slide to slide were never more than 10 per cent of the mean value for the standard kidney nuclei; such variations were compensated for by a factor of such size as to set equal the mean val ...
Direct Evidence for the Radioprotective Effect of Various
... Glucansucrases from L. mesenteroides B-512FMCM and NRRL B-1355 were prepared from culture supernatants of LW liquid cultures [5, 7]. The composition of LW medium was 0.5% yeast extract, 0.5% peptone, 2% K2HPO4, and 2% sucrose. Each 200 mM sucrose or maltose was prepared with 20 mM Na-acetate buffer ...
... Glucansucrases from L. mesenteroides B-512FMCM and NRRL B-1355 were prepared from culture supernatants of LW liquid cultures [5, 7]. The composition of LW medium was 0.5% yeast extract, 0.5% peptone, 2% K2HPO4, and 2% sucrose. Each 200 mM sucrose or maltose was prepared with 20 mM Na-acetate buffer ...
RNA synthesis/Transcription I Biochemistry 302
... specific regions/residues of β/β′ and the growing RNA chain (RBS), heteroduplex (HBS), or “downstream” DNA (DBS). • Core RNAP moves along the DNA template simultaneously unwinding DNA ahead and rewinding the template behind. Zn2+-binding domain of β′ subunit is the sliding clamp. RNAP activity requi ...
... specific regions/residues of β/β′ and the growing RNA chain (RBS), heteroduplex (HBS), or “downstream” DNA (DBS). • Core RNAP moves along the DNA template simultaneously unwinding DNA ahead and rewinding the template behind. Zn2+-binding domain of β′ subunit is the sliding clamp. RNAP activity requi ...
BB30055: Genes and genomes
... True or False All Repetitive DNA elements in the human genome arose through a common mechanism ...
... True or False All Repetitive DNA elements in the human genome arose through a common mechanism ...
MB206_fhs_lnt_007a_AT_Jan09
... content 4. Should not include palendromic sequences (will form hairpin loops) 5. Primer annealing temps within 5o C of each other 6. Should not have inter primer homologies - results in primer dimers 7. Between 40-60% GC content 8. No Poly C or G- result in non specific binding • from high bond ener ...
... content 4. Should not include palendromic sequences (will form hairpin loops) 5. Primer annealing temps within 5o C of each other 6. Should not have inter primer homologies - results in primer dimers 7. Between 40-60% GC content 8. No Poly C or G- result in non specific binding • from high bond ener ...
RF cloning: A restriction-free method for inserting target genes into
... following the primer sequence is rather high (data not shown). However, the high processivity of KOD polymerase might be useful for very long constructs. In contrast to other methods that rely on DNA amplification, the method described here does not need the subsequent cloning of the amplified gene, ...
... following the primer sequence is rather high (data not shown). However, the high processivity of KOD polymerase might be useful for very long constructs. In contrast to other methods that rely on DNA amplification, the method described here does not need the subsequent cloning of the amplified gene, ...
Rapid Method for Extraction of Genomic DNA From Vitex negundo L.
... negundo L. genomic DNA that is free from solubilising polysaccharides and polyphenols. This protocol uses NaCl, PVP, mercaptoethanol, SDS and incubation at 68°C for 1 hour. Mild temperature conditions during extraction and precipitation were also recognized as important parameters. The quantity of i ...
... negundo L. genomic DNA that is free from solubilising polysaccharides and polyphenols. This protocol uses NaCl, PVP, mercaptoethanol, SDS and incubation at 68°C for 1 hour. Mild temperature conditions during extraction and precipitation were also recognized as important parameters. The quantity of i ...
Nerve activates contraction
... 2. Restriction enzymes are used to make recombinant DNA • Gene cloning and genetic engineering were made possible by the discovery of restriction enzymes that cut DNA molecules at specific locations. • In nature, bacteria use restriction enzymes to cut foreign DNA, such as from phages or other bact ...
... 2. Restriction enzymes are used to make recombinant DNA • Gene cloning and genetic engineering were made possible by the discovery of restriction enzymes that cut DNA molecules at specific locations. • In nature, bacteria use restriction enzymes to cut foreign DNA, such as from phages or other bact ...
Chapt 10
... – An RNA molecule is transcribed from a DNA template by a process that resembles the synthesis of a DNA strand during DNA replication. – RNA nucleotides are linked by the transcription enzyme RNA polymerase. – Specific sequences of nucleotides along the DNA mark where transcription begins and ends. ...
... – An RNA molecule is transcribed from a DNA template by a process that resembles the synthesis of a DNA strand during DNA replication. – RNA nucleotides are linked by the transcription enzyme RNA polymerase. – Specific sequences of nucleotides along the DNA mark where transcription begins and ends. ...
Engineering Life: Building a Fab for Biology
... It is a way of thinking about existing biological machines and of constructing new ones. setts Institute of Technology artificial intelligence laboratory groups create the bonds between base pairs as well as between has observed, “The lack of standardization in assembly tech- adjoining nucleotides al ...
... It is a way of thinking about existing biological machines and of constructing new ones. setts Institute of Technology artificial intelligence laboratory groups create the bonds between base pairs as well as between has observed, “The lack of standardization in assembly tech- adjoining nucleotides al ...
Paper Plasmid 2 - dublin.k12.ca.us
... d. Find which RE can be used to cut both Cell DNA and plasmid so that the Cell DNA's gene of interest can be inserted into the plasmid. Remember that the protein coding sequence from the Cell DNA must not be cut nor should the replication origin or one antibiotic resistant gene be cut on the plasmid ...
... d. Find which RE can be used to cut both Cell DNA and plasmid so that the Cell DNA's gene of interest can be inserted into the plasmid. Remember that the protein coding sequence from the Cell DNA must not be cut nor should the replication origin or one antibiotic resistant gene be cut on the plasmid ...
Disclaimer: Not ALL of the questions on the midterm will necessarily
... bacterial gene has a base sequence 5’ ACAGGC. Draw and label a diagram of how this sequence would be copied during transcription. Which enzyme is responsible for transcription? And name the three dif rent molecules made by transcription. ...
... bacterial gene has a base sequence 5’ ACAGGC. Draw and label a diagram of how this sequence would be copied during transcription. Which enzyme is responsible for transcription? And name the three dif rent molecules made by transcription. ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.