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Male Karyotype - WordPress.com
Male Karyotype - WordPress.com

... Female Karyotype ...
Primer Design
Primer Design

Chapter Introduction Lesson 1 Mendel and His Peas Lesson 2
Chapter Introduction Lesson 1 Mendel and His Peas Lesson 2

... What Controls Traits • Mendel concluded that two factors—one from each parent—control each trait. • Mendel’s “factors” are part of chromosomes which exist as pairs—one chromosome from each parent. • Each cell in an offspring organism contains chromosomes from both parents. ...
How DNA Evidence Works The Science of DNA Fingerprinting
How DNA Evidence Works The Science of DNA Fingerprinting

... transfer and permanently affix DNA to a nylon membrane. First, the DNA is denatured from its native double helix into a single-stranded state (this frees up nucleotides to base-pair with DNA probes for step 5 of the process). The positively charged nylon membrane is then placed on top of the agarose ...
H - Images
H - Images

... The glycosidic bond is between the 1C-4C. The chains coil in water and don’t form H bonds, therefore not very soluble in H2O. Only 20% of starch in potatoes is amylose. 80% is amylopectin- short and branched ...
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lab9

... Separation by Size • As DNA is moved through the gel, smaller sized fragments move through faster than larger sized fragments – Ex. A 100 base pair fragment will move through the gel faster than a 500 bp fragment ...
DNA Testing Info
DNA Testing Info

... abbreviated as “R”. Thus, sheep may be referred to by their codon 171 genotype as QQ, QR or RR. Remember, sheep carry two copies of the prion protein gene (one from mother, one from father) and, therefore, the genotype must have two letters. To determine the sheep's genotype, a laboratory will isola ...
Geometrical ordering of DNA in bacteria
Geometrical ordering of DNA in bacteria

... average chromosome configuration as obtained from our theoretical model in which compacted DNA is represented by a chain of blobs. The position on the chromosome is parameterized by the contour length s (measured in units of DNA length L). The configurations shown in (A) are for different blob diame ...
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mv-lect-06-virus-repl-stratigies

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Chapter 12 Molecular Genetics

... DNA separate, serve as templates, and produce DNA molecules that have one strand of parental DNA and one strand of new DNA. ...
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pGLO Transformation SV

... In this lab, you will be using non-pathogenic E. coli bacteria and pGLO, a plasmid modified with two genes. The pGLO plasmid contains the genetic codes for (see Table 2): 1. a green fluorescent protein (GFP) from the bioluminescent jellyfish, Aequorea victoria 2. ampicillin resistance (amp) 3. a spe ...
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- Horizon Discovery

... Fixation using formalin is a critical step in the preparation of histological sections. It ensures the preservation of tissue architecture and cell morphology by cross-linking biomolecules. If fixation is not carried out under optimal conditions a tissue specimen can be irreversibly damaged. Methods ...
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DNA Sequencing Handbook

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Gene Section MRE11A (MRE11 meiotic recombination 11 homolog A (S. cerevisiae))

... domain of each Rad50 unit. As the Zinc-hook of Rad50 is located at the end of a long coiled-coil domain, this provides a flexible structure in which each DNA end is accessible to additional repair enzymes while being held in close proximity to each other in preparation for re-ligation. Cells lacking ...
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- GenoSensor Corporation
- GenoSensor Corporation

... Now, taking a look at the molecular side of things we see that the physical variations in this gene among the population are extremely tiny. How tiny? These variations are due to a single nucleotide difference in the DNA template strand that is thought to affect the protein product when its mRNA is ...
part v - dna extraction of epithelial cells
part v - dna extraction of epithelial cells

... electric field and is usually used to separate different sizes of DNA fragments for DNA analysis. 23. ________________________ to modify the molecular structure of (such as a protein or DNA) especially by heat, acid, alkali, or ultraviolet radiation so as to destroy or diminish some of the original ...
Model of unequal chromosomal crossing over in DNA sequences1
Model of unequal chromosomal crossing over in DNA sequences1

... The expansion of SSR in DNA sequences plays an important role in genome organization and evolution and is associated with many genetic diseases (e.g. see [ 3 – 5]). It was recently discovered [6,7] that the length distribution of a particular type of simple repeats, dimeric tandem repeats (DTR), can ...
The Work of Gregor Mendel
The Work of Gregor Mendel

... pairing and how the two strands of DNA are held together. The model showed the following: The two strands in the double helix run in opposite directions, with the nitrogenous bases in the center. Each strand carries a sequence of nucleotides, arranged almost like the letters in a fourletter alphabet ...
pARA-R Restriction Digest: An Introduction to Plasmids and
pARA-R Restriction Digest: An Introduction to Plasmids and

... pARA-R Restriction Digest: An Introduction to Plasmids and Restriction Enzymes Laboratory 2a ...
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Jeopardy

... $500 Question from H1 Polyploidy creates new types of organisms That are larger and stronger than their diploid Relatives in what organism? ...
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RNA and Protein Synthesis

Stereoselectivity in DNA-Templated Organic
Stereoselectivity in DNA-Templated Organic

... adjacent to either reactant and that the conformation(s) leading to stereoselectivity require g5-6 consecutive aromatic bases. We speculate that this requirement arises from the role of base stacking in stabilizing single-stranded helical conformations. Indeed, singlestranded DNA of mixed sequence h ...
LP - Columbia University
LP - Columbia University

... Sister/Sibling Chromatids: The DNA molecules in sister chromatids are identical because they are the two products of a single semi-conservative DNA replication. How many chromatids per chromosome? Can be 1 or 2; depends on where cell is in the cell cycle. Before S, each chromosome has one chromatid ...
DNA Repair: Its Importance and How to Improve it
DNA Repair: Its Importance and How to Improve it

... molecules are summoned to, and bind at or near the site of damage, inducing other molecules to bind and form a complex that enables the actual repair to take place. The types of molecules involved and the mechanism of repair that is mobilized depend on the type of damage that has occurred and the ph ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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